Aspergillus spp. is widely spread in the world on feeds and foods. They have been known to produce aflatoxins, which are mutagenic and carcinogenic to humans. The objective of this study was to determine the optimum gamma-irradiation dose for controling the growth of Aspergillus spp. to ensure safety of feeds. Four species fungal spore ($10^7$ spore/mL) exposed to 0, 1, 3, 5, 7 and 10 kGy of gamma radiation were inhibited in their growth and $AFB_1$ productivity by ${\geq}5\;kGy$. Meantime, the growth of fungal inoculated on feeds was inhibited at ${\geq}3\;kGy$. However, $AFB_1$ presented in aqueous solution was not be inactivated completely by ${\leq}10\;kGy$ irradiation. These results indicate that Aspergillus spp. on feeds could be controlled by 5 kGy gamma-irradiation but detoxification of $AFB_1$ demands a higher dose of gamma-irradiation (${\geq}10\;kGy$).
Ha, Ji-Hye;Jeong, Hyang-Suk;Jeong, Myoung-Hoon;Kim, Seung-Seop;Jin, Ling;Nam, Jong-Hyun;Hwang, Baik;Ma, Choong-Je;Lee, Hyeon-Yong
Korean Journal of Food Science and Technology
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v.41
no.5
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pp.552-559
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2009
In this study, the anticancer activity of the water extract at $100^{\circ}C$ was compared to that of the callus extracts via a ultrasonification extraction process. All the extracts were utilized to evaluate cytotoxicity, antioxidant and immune activities. The callus extracted via ultrasonification extraction showed relatively low cytotoxicity on normal human cell lines, HEK293 and HEL299, showing 13.17% and 21.78%, respectively. The callus extract has 59.82% which was similar to 61.70% for water extracts. It was also found that callus extract yielded higher nitric oxide secretion form macrophage than other extracts. The growths of both human stomach adenocarcinoma (AGS) cell and human lung carcinoma (A549) were inhibited up to 70% by adding 1.0 mg/mL of the callus extracts with ultrasonification extraction. This inhibition ratio (70%) was almost close to that of water extract. Human hepatoma carcinoma (HEP3B) cell growth was most significantly inhibited up to 75% by adding 1.0 mg/mL of callus extracts, and its selectivity was highest compared to other extracts. It indicates that the callus extracts could selectively inhibit growth of digestive system-related cancer cells. It can be also concluded from the results of this study that the callus extracts associated with ultrasonification extraction process have the potential for anticancer activity.
Oh, Sung Hwan;Choi, Kyung-Jin;Kim, Sang Yeol;Seo, Woo Duck;Han, Sang Ik;Cho, Jun Hyun;Song, You Chun;Nam, Min Hee;Lee, Chung Keun;Woo, Sunhee;Lee, Chulwon
KOREAN JOURNAL OF CROP SCIENCE
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v.60
no.2
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pp.153-166
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2015
Production of high quality pigmented rice contained with high content of anthocyanin, and polyphenol was significantly influenced by cultivation environments like transplanting date and cultivation location. This study was carried out to establish an optimum transplanting date and cultivation region to produce maximum content of anthocyanin, polyphenol and antioxidant activity of pigmented rice varieties (black, red colored). Three transplanting times (May 20, June 5, June 20) and four different sites (Miryang, Uiseong, Sangju, Bonghwa) were evaluated with five pigmented rice cultivars. Anthocyanin and total polyphenol index to average temperature during 30 days after heading (DAH) of black, red pigmented rice varieties showed that anthocyanin and total polyphenol contents were decreased by 10% and 9%, respectively, with increasing average temperature of $1^{\circ}C$. The optimum ripening temperature of the 30 DAH for the production of high anthocyanin and total polyphenol was 22 to $23^{\circ}C$ for early maturity black rice, 21 to $22^{\circ}C$ for mid-late maturity of black and red rices, respectively. On the other hand, an estimated heading date of pigmented rices in return according to the optimum ripening temperature of the 30 DAH was ranged Aug. 11 to 17 for early maturity black rice, Aug. 25 for mid-late maturity black, red rice variety in Jecheon, Aug. 27 to Sep. 2 for early maturity type, Sep. 3 to 6 for mid-late type in Daegu. It seemed that Jecheon, Boeun, Mungyeong, and Yeongju were optimum regions for cultivation of pigmented rices. The estimated sowing date of pigmented rices for high anthocyanin and total polyphenol production based on the optimum heading date was May 18 to 26 for early maturity black rice variety, April 11 for mid-late black, red variety in Jecheon, May 23 to 28 for early type, April 9 to 26 for mid-late type in Boeun, respectively.
In this study, I was focusing on LED (Light Emitting Diode) light effect in growth of chrysanthemum. For this reason, I formed six monochromatic lights (red 650 nm, 647 nm, 622 nm, blue 463 nm, 450 nm, white), six mixed lights sources red : blue (9 : 1, 8 : 2, 7 : 3, 6 : 4, 5 : 5) and 3 control beds in light sources ratio between rad : blue (8 : 2) including sun light. It was totally 15 control beds. Depending on light investigation time in growth, 6/6 (on/off) was highest in the length of plant, the number of leaves, the fresh dry and leaf area. But statistical significance wasn't accepted in general. In case of monochromatic lights, length of plant and leaf area is biggest in the Blue 450 mm and the length of root is highest in RED 650 mm. Except for this 3 measuring points (length of plant, the number of leaves and fresh weight), sun light and white was highest. Besides there are monochromatic light effect but various wavelength range in light sources are needed to crop growth. In terms of mixed light resources, except for sun light, It turned out the length of plant is highest in the highest red light rate red : blue (9 : 1), and Red : white (7 : 3) is highest in fresh weight and dry weight. The sun light is the highest one in the leaf area. The results from LED light effect in growth of chrysanthemum are obviously effect on growth and building up the shape. We need to choose suitable light sources in the monochromatic lights and mixed lights for growing high quality of chrysanthemum or Supplemental Lighting.
The core questions for determining nitrogen topdress rate (Npi) at panicle initiation stage (PIS) are 'how much nitrogen accumulation during the reproductive stage (PNup) is required for the target rice yield or protein content depending on the growth and nitrogen nutrition status at PIS?' and 'how can we diagnose the growth and nitrogen nutrition status easily at real time basis?'. To address these questions, two years experiments from 2001 to 2002 were done under various rates of basal, tillering, and panicle nitrogen fertilizer by employing a rice cultivar, Hwaseongbyeo. The response of grain yield and milled-rice protein content was quantified in relation to RVIgreen (green ratio vegetation index) and SPAD reading measured around PIS as indirect estimators for growth and nitrogen nutrition status, the regression models were formulated to predict PNup based on the growth and nitrogen nutrition status and Npi at PIS. Grain yield showed quadratic response to PNup, RVIgreen around PIS, and SPAD reading around PIS. The regression models to predict grain yield had a high determination coefficient of above 0.95. PNup for the maximum grain yield was estimated to be 9 to 13.5 kgN/10a within the range of RVIgreen around PIS of this experiment. decreasing with increasing RVIgreen and also to be 10 to 11 kgN/10a regardless of SPAD readings around PIS. At these PNup's the protein content of milled rice was estimated to rise above 9% that might degrade eating quality seriously Milled-rice protein content showed curve-linear increase with the increase of PNup, RVIgreen around PIS, and SPAD reading around PIS. The regression models to predict protein content had a high determination coefficient of above 0.91. PNup to control the milled-rice protein content below 7% was estimated as 6 to 8 kgN/10a within the range of RVIgreen and SPAD reading of this experiment, showing much lower values than those for the maximum grain yield. The recovery of the Npi applied at PIS ranged from 53 to 83%, increasing with the increased growth amount while decreasing with the increasing Npi. The natural nitrogen supply from PIS to harvest ranged from 2.5 to 4 kg/10a, showing quadratic relationship with the shoot dry weight or shoot nitrogen content at PIS. The regression models to estimate PNup was formulated using Npi and anyone of RVIgreen, shoot dry weight, and shoot nitrogen content at PIS as predictor variables. These models showed good fitness with determination coefficients of 0.86 to 0.95 The prescription method based on the above models predicting grain yield, protein content and PNup and its constraints were discussed.
These days, agricultural products cultivated in facilities occupy the highest percentage of agricultural output price. Specifically cucumbers have been one of the crops that farmers prefer to growing, because their prices were high. However, cucumber crop is sensitive to the soil and environments and it requires the exact crop management. In order to establish cultivation techniques for cucumbers, the current situation of cucumber cultivation was surveyed from ten cucumber farmlands; five farmlands of cucumber cultivation in forcing and five of semi-forcing practicing systems, respectably. The soil conditions were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy in soil texture family. Soil was managed with deep plowing combined with application of basal fertilizers such as compost, rice straw, oil cake, wood chip and chemical fertilizer. The whole soil was prepared in uniformly with rotary. Three major nutrients ($N-P_2O_5-K_2O$)of basal application were 815-464-529 kg $ha^{-1}$ in forcing and 197-135-151 kg $ha^{-1}$ in semi-forcing cultivation. Top dressing of fertilizer was supplied in fertigation system of macro and micro elements in 2~3 day interval with water irrigation. The average yields of cucumbers were $168t\;ha^{-1}$ with 381,000 thousand won $ha^{-1}$ in average gross profit (AGP) in forcing cultivation and $115t\;ha^{-1}$ with 177,000 thousand won $ha^{-1}$ in AGP in semi-forcing cultivation. Cucumber production during the winter season was considered to increase the gross profit because cucumber price tends to stay in high level during this time. The accumulation of soil chemicals like EC, available $P_2O_5$ and exchangeable cations could be controlled by rice straw application. The rice straw application increased soil temperature during the winter season, in exchange of soil air, and in extension of plant roots. In addition, the rice straw application somewhat affected decrease of salts accumulation.
Ha, Jae Jung;Oh, Dong Yep;Lee, Jea Young;Yang, Ka Young;Kim, Jong Bok;Ohh, Sang Jip;Song, Young Han
Journal of Animal Science and Technology
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v.55
no.5
/
pp.381-388
/
2013
This study examined the effects of sex in different group sizes of Hanwoo calves. The growth performance including the mean daily gain, feed conversion rate, behavioral characteristics, and blood parameters in Hanwoo calves were investigated. A total of 54 heads of Hanwoo calves were housed separately at 4 heads per group, 3 heads per group, and 2 heads per group per pen. In the heifer calves, except for those 10 months of age, the growth performance was improved by increasing the group size, which was similar to steer calves (p<0.05). The heifer calves housed in 3 heads group spent more time lying down (p<0.05). The steer calves spent more time standing in the 3 heads group, lying down (2 heads group), and walking (4 heads group). The heifer calves housed in 2 heads group had a lower frequency of drinking, self-grooming, pairwise grooming, and fighting (p<0.05). On the other hand, the steer calves in the 4 heads group had a higher frequency of drinking, rubbing, and fighting (p<0.05). The white blood cell and cortisol were highest in the heifer calves in the 2 heads group (p<0.05).
This study was carried out to investigate the effect of several organic fertilizers on the growth characteristics, root yield and the levels of the efficacious constitutents saikosaponins in Bupleurum falcatum L. in the field of Chonnam RDA. Naju, 1994. Five organic fertilizers were used ; rice straw manure(RSM), rice straw(byproduct of mushroom)manure[RS(BM)M], pig-dropping sawdust manure(PSM),poultry manure(PM)and sawdust(byproduct of mushroom) manure[S(BM)M]. The results were summarized as follows ; The chemical properties, such as organic matter, available phosphate, $K_2O$, CaO, and MgO of soil were increased on all plots with the addition of organic fertilizers, specifically in PM+RS(BM)M application. PM+RS(BM)M treatment was higher in emergence rate and better in the growth charaters of shoot, root parts of plants than those of conventional fertilization and the highest dried root yield resulted from using 39% PM+RS(BM)M treatment. Thus, the organic constituents of the plant leaves were affected in the same ways by all treatments, but the T-N, $P_2O_5$, $K_2O$, CaO and MgO contents of the roots were highly increased in PM+RS(BM)M treatment, also the total saikosaponin levels in the roots increased with different organic fertilizers in the following order : 1.70% in PM+RS(BM) treatment, 1.66% in RS(BM) treatment, and 1.57% in RSM+N-P-K treatment. There were significant positive correlation observed between the growth characters of shoot, root parts of plants, yield and contents of T-N, $P_2O_5$, $K_2O$, total-saikosaponin in B. falcatum roots.
Yoo, Young Bok;Lee, Sang Cheol;Jung, Won Soon;Jang, Kab Yeul;Kong, Won Sik;Cheong, Jong Chun;Oh, Se Jong;Jhune, Chang Sung
Journal of Mushroom
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v.6
no.2
/
pp.47-51
/
2008
The Oyster mushroom is saprophytic fungus. The pileus is stemmed at the side and later depressed. It grows to 5-15 cm, and is of grey, grey-lilac, blackish-grey, steel grey, grey-brown, and blue-blackish. Various kinds of Oyster mushrooms such as golden, pink, brown, grey, white, and blue make marketing an interesting challenge depending upon the market niche. A new commercial strain "Chung" of oyster mushroom was developed by hyphal anastomosis. It was improved with hybridization between monokaryotic strain derived from Pleurotus ostreatus ASI 2194 and ASI 2487. The pileus of parental strain ASI 2194 and ASI 2487 was grey and light blue-grey, respectively. Most of intra-specific hybrids between strain ASI 2194 and ASI 2487 were showed similar pileus color of parents. By the way, the pileus color of variety 'Chung' was blue to bluish grey. The optimum temperatures of mycelial growth and fruiting body development was $25{\sim}30^{\circ}C$ and $12{\sim}16^{\circ}C$, respectively.
It is very important to make shorter and healthier pot plants with increased numbers of branch at a growing stage before short-day exposure. Especially light and nutrient conditions directly affect the growth and quality of the plants as described above. In this study, the effects of nutrient strength and light intensity on the nutrient uptake and growth of young Kalanchoe plants (Kalanchoe blossfeldiana 'Marlene') during this growth stage were investigated. The plants were grown under two radiation integral (15.8 and 7.9 $mol{\cdot}m^{-2}{\cdot}d^{-1}$, PPF) and three EC (0.8, 1.6 and 2.4 $dS{\cdot}m^{-1}$) conditions. Leaf area, fresh weight, dry weight and number of branch were higher at a higher PPF, and this tendency was more evident at an EC above 1.6$dS{\cdot}m^{-1}$. The plants became higher at a lower PPF. When the EC was at 0.8 $dS{\cdot}m^{-1}$, the plants did not grow so healthy regardless of PPF conditions. EC decrement in the nutrient solution was increased with increase of nutrient strength. With growth stage, the nutrient uptake was increased with increases of nutrient strength and PPF. At a higher PPF, $NO_3-N,\;K^{+}\;and\;Ca^{2+}$ were much more absorbed, and especially the uptake of $K^{+}$ was 1.1 to 1.5 times greater than that or $NO_3-N$. From the results, the EC needed above 1.6 $dS{\cdot}m^{-1}$ during the seedling stage in order to make more healthy Kalanchoe plants having more leaf area, fresh weight, dry weight and number of branches under adequate light conditions.
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