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Comparison of Anticancer Activities of Ultrasonification Extracts of Callus and Roots from Rhodiola sachalinensis A. Bor  

Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University)
Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National University)
Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National University)
Kim, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National University)
Jin, Ling (College of Bioscience and Biotechnology, Kangwon National University)
Nam, Jong-Hyun (Grami Co., Ltd.)
Hwang, Baik (Department of Biology, Chonnam National University)
Ma, Choong-Je (College of Bioscience and Biotechnology, Kangwon National University)
Lee, Hyeon-Yong (Research Institute of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.41, no.5, 2009 , pp. 552-559 More about this Journal
Abstract
In this study, the anticancer activity of the water extract at $100^{\circ}C$ was compared to that of the callus extracts via a ultrasonification extraction process. All the extracts were utilized to evaluate cytotoxicity, antioxidant and immune activities. The callus extracted via ultrasonification extraction showed relatively low cytotoxicity on normal human cell lines, HEK293 and HEL299, showing 13.17% and 21.78%, respectively. The callus extract has 59.82% which was similar to 61.70% for water extracts. It was also found that callus extract yielded higher nitric oxide secretion form macrophage than other extracts. The growths of both human stomach adenocarcinoma (AGS) cell and human lung carcinoma (A549) were inhibited up to 70% by adding 1.0 mg/mL of the callus extracts with ultrasonification extraction. This inhibition ratio (70%) was almost close to that of water extract. Human hepatoma carcinoma (HEP3B) cell growth was most significantly inhibited up to 75% by adding 1.0 mg/mL of callus extracts, and its selectivity was highest compared to other extracts. It indicates that the callus extracts could selectively inhibit growth of digestive system-related cancer cells. It can be also concluded from the results of this study that the callus extracts associated with ultrasonification extraction process have the potential for anticancer activity.
Keywords
Rhodiola sachalinensis A. Bor; callus; ultrasonification extraction; anticancer activity; cytotoxicity;
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1 Lee KJ, Um BH. Extraction of useful component from natural plants using ultrasound system. Korean J. Biotechnol. Bioeng. 23: 101-108 (2008)   과학기술학회마을   ScienceOn
2 Lee EJ, Im JS, Park CK, Jeon BS, Kim SC. Food components and volatile flavors in Rhodiola sachalinensis roots. Food Indus. Nutr. 9: 53-57 (2004)   과학기술학회마을   ScienceOn
3 Kim JY, Seong NS, Lee YJ. A study on the effects of Rhodiola rosea root on the cancers. Korean J. Herbology. 21: 79-87 (2006)   과학기술학회마을   ScienceOn
4 Bu YM, Baek SK, Jin ZH, Kang TH, Rho SJ, Kim HC. Neuroprotective effect of Rhodiola sachalinensis extracts on transient focal cerebral ischemia in rats. Korean J. Herbol. 19: 191-197 (2004)
5 Oh IS, So SS, Xu MZ. Growth and utility of Rhodiola sachalinensis in Baekdu mountain I. induction of callus and composition of free amino acids. Korean J. Environ. Biol. 22: 148-152 (2004)   과학기술학회마을
6 Doll R, Peto R. The causes of cancer, quantitative extimates of avoidable risks of cancer in the United States today. J. Nat. Cancer Inst. 66: 1192 (1981)
7 Jung WK, Lee DY, Kim JH, Choi IH, Park SG, Seo SK, Lee SW, Lee CM, Park YM, Jeon YJ, Lee CH, Jeon BT, Qian ZJ, Kim SK, Choi IW. Anti-inflammatory activity of caffeic acid phenethyl ester (CAPE) extracted from Rhodiola sacra against lipopolysaccaride-induced inflammatory responses in mice. Process Biochem. 43: 783-787 (2008)   DOI   ScienceOn
8 Jung HJ, Choi YJ, Won CW, Seo YR. The Chemopreventive effects of antioxidant enzyme. Environ. Mutagen. Carcinogen. 26: 45-47 (2006)   과학기술학회마을   ScienceOn
9 Ha H, Yoon SH, Takeru FJ, Hitoshi HR. MTT assay using primary cultured rat hepatocytes. Korean J. Environ. Toxicol. 9: 19-23 (1994)   과학기술학회마을   ScienceOn
10 Cui SB, Lee DS, Ham SS. Antioxidative, antimutagenic, and cytotoxic effects of Rhodiola sachalinensis extract. J. Korean Soc. Food Sci. Nutr. 32: 211-261 (2004)
11 Park JG, Kim SW. Molecular study of aging by free radical. J. Basic Sci. 9: 103-114 (1998)
12 Yu CY, Kim SH, Lim JD, Kim MJ, Chung IM. Intraspecific relationship analysis by DNA markers and in vitro cvtotoxic and antioxidant activity in Eleutherococcus senticosus. Toxicol. In vitro 17: 229-236 (2003)   DOI   ScienceOn
13 Kim BS. The management of lung cancer. Family Physician 6: 8 (1985)
14 Kim JY, Lee YJ. A study on the effects of Rhodiola rasea root on the immune system. Korean J. Herbol. 23: 179-189 (2008)
15 Park KU, Yoon JH, Kim JY, Jeong CH, Park CK, Song WS, Seo KI. Biological activity of the fractions extracted from Rhodiola dumulosa. Korean J. Food Preserv. 12: 496-500 (2005)   과학기술학회마을   ScienceOn
16 Lee JJ. Shin DH, Park SE, Kim WI, Park DI, Choi YH, Hong SH. Euphorbiae humifusae sensitizes apoptosis of TRAIL-resistant human gastric adenocarcinoma AGS. J. Life Sci. 18: 120-128 (2008)
17 Kim SJ, Hwang B, Hwang SJ, Ahn JC. Production of salidroside from callus culture of Rhodiola sachalinensis A. Bor. Korean J. Plant Biotechnol. 31: 89-94 (2004)   과학기술학회마을   DOI
18 Bae SJ. Anticarcinogenic and antioxidant effects of Rhodiola sachalinensis. J. Korean Soc. Food Sci. Nutr. 34: 1302-1307 (2005)   과학기술학회마을   DOI   ScienceOn
19 Choi BK, Kim SW. Studies on the regulation of nitric oxide synthesis in murine mononuclear phagocytes. Korean J. Environ. Toxicol. 15: 69-80 (2000)   과학기술학회마을   ScienceOn
20 Wang HB, Ding YY, Zhou J, Sun XL, Wang SW. The in vitro and in vivo antiviral effects of salidroside from Rhodiola rosea L. against coxsackievirus B3. Phytomedicine 16: 146-155 (2009)   DOI   ScienceOn
21 Park JW. New targeted agents for hepatocellular carcinoma. Korean J. Liver Cancer Study Group 8: 16-17 (2008)
22 Kim CH, Kwon MC, Han JG, Ha JH, Jeong HS, Choi GP, Park UY, Nam JH, Hwang B, Lee HY. Immune activities of Rhodiola sachalinensis A. Bor. extracts isolated with various extraction process. Korean J. Med. Crop Sci. 16: 383-389 (2008)
23 Lee DI, Kim HK, Lee MW, Choi YW, Kim HH, Kim EJ. Augmentation of macrophage cytotoxicity and NO production by pedunculagin. Yakhak Hoeji 44: 174-181 (2000)
24 Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ. Antioxidant activity of black garlic (Allium sativum L.). J. Korean Soc. Food Sci. Nutr. 37: 965-971 (2008)   DOI   ScienceOn
25 Chen JH, Ho CT. Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. J. Agr. Food Chem. 45: 2374-2378 (1997)   DOI   ScienceOn
26 Kim SJ, Kim KS, Hwang SJ, Chon SU, Kim YH, Ahn JC, Hwang B. Identification of salidroside from Rhodiola sachalinensis A. Bor. and its production through cell suspension culture. Korean J. Med. Crop Sci. 12: 203-208 (2004)   과학기술학회마을   ScienceOn
27 Hong EY, Kim IH, Yun JS, Yun T, Lee CH. Growth characteristics and search for eligible cultivation area of Rhodiola sachalinensis A. Boriss. Korean J. Plant Res. 16: 212-217 (2003)
28 Choi BK, Kim SW. Studies on the regulation of nitric oxide synthesis in murine mononuclear phagocytes. Korean J. Environ. Toxicol. 15: 69-80 (2000)   과학기술학회마을   ScienceOn
29 Ma L, Cai D, Li HX, Tong BB. Song LH, Wang Y. Anti-fatigue effects of salidroside in mice. J. Med. Coll. PLA 23: 88-93 (2008)   DOI   ScienceOn
30 Kang MY, Lee YR, Koh HJ, Nam SH. Antioxidative and antimutagenic activity of ethanolic extracts from Glant embroynic rices. J. Korean Soc. Appl. Biol. Chem. 47: 61-66 (2004)
31 Kwon MC, Kim CH, Kim HS, Lee SH, Choi GP, Park UY, You SG, Lee HY. Optimal extract condition for the enhancement of anticancer activities of Aftemisia prinsceps Pampanini. Korean J. Medical Crop Sci. 15: 233-240 (2007)