• Title/Summary/Keyword: 고정-고정 보

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Production of Chitosna Oligosaccharides Using Chitin-Immobilized Enzyme (키틴 고정화 효소를 이용한 키토산 올리고당의 생산)

  • 전유진;박표잠;변희국;송병권;김세권
    • KSBB Journal
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    • v.13 no.2
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    • pp.147-154
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    • 1998
  • Enzymatic hydrolysis using an immobilized enzyme was carried out to produce chitosan oligosaccharides (COSs) from chitosan effectively. Chitosanase was immobilized on eight different carriers by physical adsorption. The enzyme immobilized on chitin had higher activity than those immobilized on the other carriers in spite of its lower adsorption. The activity of chitin-immobilized enzyme was more than 90% of the original activity. Optimal temperature of the immobilized enzyme increased by about $15^{\circ}C$ and its thermostability was excellent in relatively wide range of temperature. But its effects of pH did not improve compared to the free enzyme. The immobilized enzyme produced 153 mg/g chitosan of the reducing sugar for 3hrs of hydrolytic incubation time. The total content of higher oligomers, tetramer to hexamer, among amount of total COSs obtained for 2hrs was more than 90%. In kinetic parameters for both enzymes, immobilized enzyme showed lower affinity for substrate and reaction rate than free enzyme, however, no reduction of the rate for high substrate concentrations. Consequently, chitin-immobilized could effectively hydrolyse chitosan and produce the higher COSs without activity decrease in comparison with the free enzyme.

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Effect of Stereotype Threat on Spatial Working Memory and Emotion Recognition in Korean elderly (노화에 대한 고정관념 위협이 노인의 공간 작업기억 및 정서인식에 미치는 영향)

  • Lee, Kyoung eun;Lee, Wanjeoung;Choi, Kee-hong;Kim, Hyun Taek;Choi, June-seek
    • 한국노년학
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    • v.36 no.4
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    • pp.1109-1124
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    • 2016
  • We examined the effect of stereotype threat (STT) on spatial working memory and facial emotion recognition in Korean elderly. In addition, we investigated the role of expected moderator such as self-perception of aging. Seventeen seniors (male=7) received basic cognitive tests including K-WMS-IV, MMSE and answered self-report questionnaires including self-perception of aging, anxiety of aging, attitude toward aging and age identity on the first visit. On the second visit, they were exposed to negative stereotype by reading a script detailing cognitive decline related to aging while a control group was exposed to a neutral content. Following the exposure, they were tested on a spatial-working memory task (Corsi-block tapping task) and emotion recognition task (facial expression identification task). The results showed that the seniors exposed to STT showed significantly lower performance on emotion recognition task (p < .05) (i.e., especially on the more difficult facial stimuli). In addition, there was a significant interaction between STT and self-perception of aging (p< .05), indicating that those who have positive self-perception of aging did not show impairment in emotion recognition task and difficult spatial working memory task under STT. On the other hand, those with negative self-perception of aging showed impaired performance under STT. Taken together, the current study suggests that being exposed to STT could negatively influence cognitive and emotional functioning of elderly. Interestingly, having a positive self-perception of aging could protect the underperformance caused by STT.

Clinical Implementation of an Eye Fixing and Monitoring System with Head Mount Display (Head Mount Display (HMD)를 이용한 안구의 고정 및 감시장치의 임상사용 가능성 확인)

  • Ko, Young-Eun;Park, Seoung-HO;Yi, Byong-Yong;Ahn, Seung-Do;Lim, Sang-Wook;Lee, Sang-Wook;Shin, Seong-Soo;Kim, Jong-Hoon;Choi, Eun-Kyung;Noh, Young-Ju
    • Progress in Medical Physics
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    • v.18 no.1
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    • pp.1-6
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    • 2007
  • A system to non-invasively fix and monitor eye by a head mounted display (HMD) with a CCD camera for stereotactic radiotherapy (SRS) of uveal melanoma has been developed and implemented clinically. The eye fixing and monitoring system consists of a HMD showing patient a screen for fixing eyeball, a CCD camera monitoring patient's eyeball, and an immobilization mask. At flrst, patient's head was immobilized with a mask. Then, patient was Instructed to wear HMD, to which CCD camera was attached, on the mask and see the given reference point on its screen. While patient stared at the given point in order to fix eyeball, the camera monitored Its motion. Four volunteers and one patient of uveal melanoma for SRS came into this study. For the volunteers, setup errors and the motion of eyeball were analyzed. For the patient, CT scans were peformed, with patient's wearing HMD and fixing the eye to the given point. To treat patient under the same condition, daily CT scans were also peformed before every treatment and the motion of lens was compared to the planning CT Setup errors for four volunteers were within 1mm and the motion of eyeball was fixed within the clinically acceptable ranges. For the patient with uveal melanoma, the motion of lens was fixed within 2mm from daily CT scans. An eye fixing and monitoring system allowed Immobilizing patient as well as monitoring eyeball and was successfully implemented in the treatment of uveal melanoma for SRS.

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Long-term teaching Characteristics of Hexavalent Chromium Contaminated Soils Treated with Phosphate and Chromium Reducing Agent (크롬환원제와 인산염으로 처리된 6가 크롬 오염토양의 장기용출 특성)

  • Lee Eui-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.3
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    • pp.448-452
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    • 2006
  • This study was performed to evaluate the long-term teachability of the stabilized hexavalent chromium contaminated soils treated with chromium reducing agent and soluble phosphate. The study shows that $Cr^{6+}$ in the leachate was little detected and the remaining $PO_4-P$ concentration kept below 0.1 mg/L due to the formation of geochemically stable hexavalent chromium phosphate minerals from the reactions of labile soil $Cr^{6+}$ forms with the added soluble phosphate salt and chromium reducing agent. The results provide evidence for the potential use of the immobilization technique for the hexavalent chromium contaminated soil remediation in the near future.

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In-plane buckling strength of fixed parabolic arch (고정지점 포물선 아치의 면내 좌굴강도)

  • Moon, Ji Ho;Yoon, Ki Yong;Cho, Yong Rae;Lee, Hak Eun
    • Journal of Korean Society of Steel Construction
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    • v.18 no.3
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    • pp.301-310
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    • 2006
  • If arches are braced by lateral restraints, the ultimate strength of arches is determined by in-plane buckling and plastic bending collapse. This paper is conducted to investigate the in-plane nonlinear elastic and inelastic buckling behavior and the strength of fixed parabolic arches in uniform compresion, as well as to study arch behaviors against non-uniform in-plane compression and bending. As shown by the results, the limit slenderness ratio is suggested to classify the bucklingmode. Buckling strength of fixed parabolic arches under uniform compresion are evaluated using buckling curve for a straight column. Finally, an interaction e quation for arches under combined axial compresion and bending action is proposed.

Enhancing Fixed Priority Scheduling Algorithms for Real-Time Tasks on Multiprocessors (다중처리기 상의 실시간 태스크를 위한 고정 우선순위 스케줄링 알고리즘의 성능 향상)

  • Park Minkyu;Han Sangchul;Kim HeeHeon;Cho Seongje;Cho Yookun
    • Journal of KIISE:Computing Practices and Letters
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    • v.11 no.1
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    • pp.62-68
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    • 2005
  • This paper presents a scheme to enhance fixed priority scheduling algorithms on multiprocessors. This scheme gives the highest priority to jobs with zero laxity and schedules them Prior to other jobs. A fixed priority algorithm employing this scheme strictly dominates the original one; it can schedule all task sets schedulable by the fixed priority algorithm and some task sets not schedulable by the fixed priority algorithm. Simulation results show that the proposed scheme improves fixed priority algorithms in terms of the number of schedulable task sets and schedulable utilization bound.

Communication Protocol for Mobile Sensor Networks (이동 센서 네트워크를 위한 통신 프로토콜)

  • Kim, Hyoung-Jin;Kim, Lae-Young;Song, Joo-Seok
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.10d
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    • pp.395-398
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    • 2006
  • 최근 Robomote, Robotic Sensor Agents(RSA)와 같은 이동 센서의 등장으로 인해 이동 센서네트워크(MSN: Mobile Sensor Network)에 대한 연구가 활발히 진행되고 있다. 하지만 기존의 이동 센서네트워크에 대한 연구는 주로 기존의 고정 센서네트워크(SSN: Stationary Sensor Network)에서 발생하는 문제점인 coverage hole을 해결하는데 초점을 맞추고 있다. 이러한 연구들에서는 이동 센서들에게 부여된 이동 능력을 최대한 활용하지 못하는 단점을 안고 있다. 이를 해결하기 위해 이동 센서에게 지속적인 이동성을 부여함으로써 고정 센서네트워크에 비해 더 넓은 영역을 센싱하도록 제안한 연구가 있으나, 그 연구가 아직 초기 단계로써 이동 센서의 지속적인 이동으로 인한 싱크 노드로의 통신 경로 설정 및 데이터 전송 문제에 대해서는 논하고 있지 않다. 이에 본 논문에서는 지속적인 이동성을 갖는 이동 센서로 구성된 이동 센서네트워크 환경에서 효율적으로 경로 설정 및 데이터 전송을 가능하게 하는 통신 프로토콜을 제안한다. 제안하는 프로토콜에서는 이동 센서와 함께 고정 센서를 배치함으로써 고정 센서가 이동 센서를 대신하여 싱크 노드로 센싱 데이터를 전송하도록 한다. 시뮬레이션을 이용한 성능 평가를 통해 제안한 통신 프로토콜이 기존의 고정 센서네트워크에 비해 센싱 영역 성능에서 우수함을 보여준다.

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Analytical Structural Stability Evaluation for H-section Beams Made of Ordinary Structural Steels Based on Boundary Conditions at High Temperatures (일반 구조용 강재 적용 정정 및 부정정 보부재의 고온 시 해석적 내력 평가 연구)

  • Kwon, In-Kyu
    • Fire Science and Engineering
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    • v.29 no.4
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    • pp.33-38
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    • 2015
  • Loads applied on the floor are transferred through beams to columns. The beams can be designed as both end fixed or simple beams. The load bearing capacity of a beam depends on each boundary condition. However, when the load bearing capacity of a beam is evaluated in fire tests, all kinds of beams are tested using simple beam conditions. In this study, an analytical method performed using heat transfer theory and heat stress analysis based on the mechanical and thermal properties of SS-400 steel at high temperature. This method was used to clarify the differences between the two types of boundary conditions at normal and high temperature. The results show that the load bearing capacity of a both-end fixed beam at high temperature is superior to that of a simple beam. Therefore, the application of simple beam conditions in fire tests for evaluation of load bearing capacity is conservatively safe compared to fixed boundary conditions.

Out of Plane Free Vibrations of Circular Curved Beams (원호형 곡선보의 면외 자유진동에 관한 수치해석적 연구)

  • 이병구;오상진
    • Computational Structural Engineering
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    • v.9 no.1
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    • pp.133-139
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    • 1996
  • In this paper, an approximate method is developed to obtain the natural frequencies of the out of plane vibration of circular curved beams. The governing differential equations are derived using the dynamic equilibrium equations with the Timoshenko theory, and solved numerically. The Runge-Kutta method and Regula-Falsi method are used to integrate the differential equations and to determine the natural frequencies, respectively. In numerical examples, the hinged-hinged and clamped-clamped end constraints are considered. For each case, the four lowest natural frequencies are reported as functions of four non-dimensional system parameters.

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Studies on the Immobilization of Enzymes and Microoganism Part 1. Immobilizing Method of Glucose Oxidase by Gamma Radiation (효소 및 미생물의 고정화에 관한 연구 제1보. 방사선조사에 의한 Glucose Oxidase의 고정화법)

  • Kim, Sung-Kih
    • Microbiology and Biotechnology Letters
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    • v.7 no.1
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    • pp.1-8
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    • 1979
  • A new method for immobilization of glucose oxidate by the aerobic gamma radiation of synthetic monomers was developed. The radiocopolymerization was conducted aerobically at -70 to -8$0^{\circ}C$ with the mixture of several polyfunctional esters, acrylates and native enzyme. The retained activity of immobilized glucose oxidase was about 50 to 55% when a NK 23G ester, acrylamide-bis and water mixture (1:1:2) in cold toluene treated with 450 krad of gam-ma radiation. The radiation dose did not influence significantly to the enzyme activity. The solvents used to prepare the beads of glucose oxidase and monomers were toluene, n-hexane, petoleum ether and chloroform. 0.05M tris-gycerol (pH 7.0) was a more suitable bugger solution for immobilizing the enzyme than was 0.02M phosphate. Immobilization of glucose oxidase shifted the optimum pH for its reaction from 6.0 to 6.5. The pH profile for the immobilized enzyme showed a broad range of optimum activity while the native enzyme gave a sharp pick for its optimum pH value. The immobilized enzyme reaction temperature was at the range of 30~4$0^{\circ}C$.

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