• The Korean Journal of Nuclear Medicine
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• v.34 no.2
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• pp.144-153
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• 2000

Purpose: The purpose of this study was to evaluate the relationship between radiation-induced activation of DNA repair genes and radiation induced apoptosis in A431 cell line. Materials and Methods: Five and 25 Gys of gamma radiation were given to A431 cells by a Cs-137 cell irradiator. Apoptosis was evaluated by flow cytometry using annexin V-fluorescein isothiocyanate and propidium iodide staining. The expression of DNA repair genes was evaluated by both Northern and Western blot analyses. Results: The number of apoptotic cells increased with the increased radiation dose. It increased most significantly at 12 hours after irradiation. Expression of p53, p21, and hRAD50 reached the highest level at 12 hours after 5 Gy irradiation. In response to 25 Gy irradiation, hRAD50 and p21 were expressed maximally at 12 hours, but p53 and GADD45 genes showed the highest expression level after 12 hours. Conclusion: Induction of apoptosis and DNA repair by ionizing radiation were closely correlated. The peak time of inducing apoptosis and DNA repair was 12 hours in this study model. hRAD50, a recently discovered DNA repair gene, was also associated with radiation-induced apoptosis.

• Korean journal of applied entomology
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• v.23 no.3 s.60
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• pp.153-157
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• 1984

The present work was designed to study the yield loss on rice at different appearance days of hopperburn caused by the brown planthopper(BPH), Nilaparvata lugens Stal, when rice plants were infested with BPH on booting and heading stages. Dead plants were colleted from 23 days after heading stage with intervals of 5 days. Yield losses by the initation of BPH infestation was greater at tooting than at heading stage. Compared to the uninfested plot, there was a reduction in 1,000 grain weight and filled grain percentage at both stages. Positive relationship was observed between rice yield(Y) and the number of days from heading to the appearance of the hopper-burn. Regression equations calculated were; for BPH feeding from booting $\hat{Y}(g)=10.145X-16.374(r=0.9726^{**})$, and for BPH feeding from heading $\hat{Y}(g)=9.792X+26.936(r=0.8850^{**})$.

• (The)Study of the Eastern Classic
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• no.54
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• pp.183-211
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• 2014

This article is to search the aspects of realization and the characteristics of Gasa, Sijo, Japga among the Korean poetry genre based on Chohan-gosa. Chohan-gosa is a basic literary and historic discourse for the medieval intellectuals. The story had been realized its own literary value at almost every genres such as Chinese literature, novels, Gasa, Japga and Sijo etc. I focused on Gasa, Sijo and Japga among the genres in this article. First of all, I subjected to Wumiin-ga enjoyed in a area of boudoir culture from Gasa based on Chohan-gosa. I catch the meaning that the text has a characteristic that popularized history and normative ideology are mingled in through it. In the case of Sijo, I focused on the Sijo which Xiang Yu is appeared in because Xiang Yu has been most quoted person from people of Chohan-gosa. Xiang Yu was described as a strong man[hero] or a man who part with the beauty Wu. I understand that this point is caused by the theatricalized characteristic of the place where Sijo have been played. For the last time, Japga is played with the characteristic of stimulative reediting of the familiar normative ideology. Chohan-ga is based on contexts of former discourse eohan-yeonui but it was not plagiarized. Chohan-ga was organized for the way which attracted the interest of the public and stimulated the emotion of the public through using popular imagery or intensifying the sorrow.

• Clinical and Experimental Pediatrics
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• v.46 no.7
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• pp.702-709
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• 2003

Purpose : $IFN{\gamma}$ sentitizes many tumor cells to $TNF{\alpha}$ and FASL-mediated apoptosis by enhancing the expression of TNF or FAS/CD95 receptor and modulating the activation of caspase and Bcl-2 family. It has been reported that $IFN{\gamma}$ and $TNF{\alpha}$ synergistically caused differentiation and growth inhibition of neuroblastoma cells. Even though some neuroblastoma cell express FASR/FASL on the cell surface, they could not induce apoptosis by ligation of the FAS/CD95 receptor. But the treatment of $IFN{\gamma}$ is reported to induce apoptosis in some neuroblastoma cell lines through the CD95/CD95L autocrine circuit. In this study, we examined whether $IFN{\gamma}$ could affect $TNF{\alpha}$ and agonistic FAS/CD95 antibody(CH-11)-induced apoptosis against neuroblastoma cell lines that had shown diverse drug sensitivity and resistance. Methods : CHLA-15, CHLA-90 and LA-N-2 neuroblastoma cells were cultured in IMDM and treated with recombinant $IFN{\gamma}$, $TNF{\alpha}$ and CH-11 antibody. Cell viability was measured by DIMSCAN with a fluorescent calcein-AM. Apoptosis was analyzed through flow cytometry using Annexin V-PE and 7-ADD staining and confirmed by pancaspase and caspase-8 blocking experiments. The expression of TNF RI and FAS/CD95 receptor was evaluated by flow cytometry using the corresponding antibody and PE-conjugated secondary antibody. Results : $IFN{\gamma}$ or $TNF{\alpha}$ alone had no demonstrable cytotoxic effects, whereas both cytokines in combination induced apoptosis synergistically in CHLA-15 and CHLA-90 cells. Although there was no cytotoxicity with the ligation of CH-11 alone in CHLA-90 cells, pretreatment of $IFN{\gamma}$ increased the sensitivity of CH-11-mediated apoptosis. The expression of TNFRI and FAS/CD95R were non-specifically enhanced after treatment of $IFN{\gamma}$ without relation to sensitivity to $TNF{\alpha}$ and CH-11. This finding suggest up-regulation of both receptors may contribute to sensitization of $TNF{\alpha}$ and CH-11-mediated apoptosis by $IFN{\gamma}$ in only sensitive cell lines. Conclusion : $IFN{\gamma}$ induced sensitization of $TNF{\alpha}$ and agonistic FAS/CD95 antibody-mediated apoptosis on some neuroblastoma cells through up-regulation of TNFRI and FAS/CD95 receptor.

• The Korean Journal of Nuclear Medicine
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• v.33 no.3
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• pp.306-315
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• 1999

Purpose: We performed this study to evaluate the process of radiation induced apoptosis in A431 skin epithelial cancer cell line. Materials and Methods: Low to high dose radiation (0, 2, 5, 10, 25 Gy) was given to A431 cells by Cs-137 cell irradiator. Apoptosis was evaluated by cell morphology, dye exclusion test, and DNA laddering. Results: Cell viability decreased as the radiation dose increased. Number of apoptotic bodies increased as radiation dose increased. It increased most significantly at 12 hours after irradiation. Lactate dehydrogenase activity in culture medium increased according to radiation dose and time after irradiation. DNA ladders could be identified in irradiated cells, but, it had no correlation with radiation dose or time after irradiation. Conclusion: Radiation-induced apoptosis which was the main course of cell death in A431 cells could be analyzed quantitatively by counting apoptotic bodies under microscope. Apoptosis increased as radiation dose increased.

• The Journal of the Korea Contents Association
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• v.12 no.2
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• pp.339-348
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• 2012

Acute promyelocytic leukemia (APL) is a cancer of the blood and bone marrow. Although all-trans retionic acid (ATRA) is the agents for ALP therapy, there are various side effects. For overcome this problem, we need the development of new therapeutic agents for APL. A number of bacteria produce various virulence factors with cytotoxic effects on human cancer cells. To understand the anti-cancer effect of Listeria monocytogenes on APL, we examined alteration of the cell viability, apoptosis and cell cycle arrest of the human promyelocytic leukemia cell line, HL-60 cells. The cell supernatant (LmSup) and the extract of L. monocytogenes (LmE) inhibited the cell viability and induced apoptosis of HL-60 cells. These cytotoxic effect of LmSup and LmE mediated by modulation of cell cycle and ROS production. These results indicate that released or included bacterial molecules from L. monocytogenes have a cytotoxicity in HL-60 cells. Therefore, LmSup and LmE may be used as the potential target for the treatment of cancer induced by HL-60 cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.252-261
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• 2003

Although the young spike of barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.) is known as the most susceptible part to spring cold injury, the risk of cold injury is apt to be ignored in most breeding program due to the importance of early maturity. Based on these aspects, the types and inducing time, temperature conditions for induction and effects of cold injury on growth and yield in this study were investigated under greenhouse and field conditions through three years (1997-1999). In natural condition, low temperature around -2.4∼$-10.2^{\circ}C$ caused the death of plant. Several cold injury types such as partial degeneration of spike, partial discoloration of leaf, spike and awn, discoloration of culm and white spike were observed at low temperature around $-3.1^{\circ}C$. Low temperature around -2.4∼$-8.6^{\circ}C$ and 1.3-$7.6^{\circ}C$ caused degeneration and sterility of spike, respectively. Most materials were prepared to the spikelet foundation stage, spikelet differentiation stage, development stage of flower organ, booting stage and heading stage, which were known having risk for cold injury in field condition. Although most of the controlled stages were sensitive to the induced low temperature, booting stage was the most sensitive stage for cold injury. All of growth stages which were treated-heading stage, booting stage, development stage of flower organ, spikelet differentiation stage, spikelet foundation stage-were responded to low temperature treatment but the symptoms revealed were very specific according to the growth stages. Ears of plant in heading stage were discolored to white. Ears of plant in booting stage were degenerated in all or part of one. Plants in spikelet differentiation stage were sterile in all or part of one. When tried to detect the specific differences between normal and cold injured plants in appearance, spike length, distance between spike and flag leaf and the first internode length could be the critical points for occurrence of spike death caused by cold injury. In barley, the elongation of spike was stopped on 3.2cm after occurrence of spike degeneration, 4.7cm after occurrence of partial degeneration of spike, 5.0cm after occurrence of white spike. In wheat, it was stopped on 1.6cm after occurrence of stem death, 3.3cm after occurrence of spike degeneration, 8.3cm after occurrence of partial degeneration of spike, 8.1cm after occurrence of white spike, 7.5cm after partial discoloration of leaf and 9.3cm after partial discoloration of spike. The obtained results from low temperature treatment induced in growth chamber were similar to the field experiment, Beacuse the death of spikes was more when low temperature was treated two times than one times, the temperature should be upgrade to -3$^{\circ}C$ in order to get the same condition with field test.

• Korean Journal of Social Welfare Studies
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• v.48 no.2
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• pp.303-329
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• 2017

This study aimed to explore the trajectories of computer game use of school age children and to identify the related predictors. The data for this study used Korean Children and Youth Panel data covering from the second year to the sixth year of elementary school. A total of 1,959 participants were analyzed. Latent class growth model was employed to explore the trajectories of computer game use and multinomial logistic regression was conducted to identify the significant predictors. Main results indicated that three types of trajectories were identified: low game using group, high initial using-fluctuating group, and high increasing game using group. Each group was found to be associated deferentially with sex, aggression, attention deficit, main caregiver's education, siblings, parent absence after-school, neglecting, family income, family trip, school grades, and peer relationship. Based on these findings, this study emphasized the importance of predictive intervention for the game user among early school age children and suggested useful practical strategies.

• Korean Journal of Poultry Science
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• v.15 no.4
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• pp.309-328
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• 1988

우리나라 양계업도 기초단계에서 발전단계를 거쳐 완전한 한 개의 산업으로서 정립할 단계에 와있다. 모든 산업이 다 이런 과정을 거처 도약하고 있으니 양계산업도 예외는 될 수 없을 것이다. 다시 말하면 정비상완기에 돌입하였다는 말이요 만약 촌시라도 정비보완을 게을리 한다면 차기도약은 불가망일 것이며 아깝게도 우리의 땀과 열성으로 길러온 양계업은 빛도 못 본체 고사하고 마는 중대한 전환기라 하겠다. 보라！(중략)

• Journal of Educational Research in Mathematics
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• v.24 no.4
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• pp.573-594
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• 2014

Capacity of making assessment items among teacher's assessment competency is essential element in validity and reliability of assessment. Errors in test items on the formal and content aspects can be a big stumbling block to derive a correct evaluation result. Through this assessment, the positive feedback can not be derived. In this study, By analyzing midterm and final 60 exam papers for geometry and vector in 45 high Schools of Seoul GyeonggiIncheon area, We argue error items and improvements on the formal and content aspect the paper-based assessment of mathematics. Through this, Our intention is to improve the skills of teachers for making assessment items.