This study aim to investigate fundamentally the growth and physiological responses of tomato plants in responses to two different levels of water deficit, a weak drought stress (-25 kPa) and a severe drought stress (-100 kPa) in soil. The two levels of water deficit were maintained using a micro-irrigation system consisted of soil sensors for the real-time monitoring of soil water content and irrigation modules in a greenhouse experiment. Soil water contents were fluctuated throughout the 30 days treatment period but differed between the two treatments with the average -47 kPa in -25 kPa set treatment and the -119 kPa in -100 kPa set treatment. There were significant differences in plant height between the two different soil water statuses in plant height without differences of the number of nodes. The plants grown in the severe water-deficit treatment had greater accumulation of biomass than the plants in the weak water-deficit treatment. The severe water-deficit treatment (-119 kPa) also induced greater leaf area and leaf dry weight of the plants than the weak water-deficit treatment did, even though there was no difference in leaf area per unit dry weight. These results of growth parameters tested in this study indicate that the severe drought could cause an adaptation of tomato plants to the drought stress with the enhancement of biomass and leaf expansion without changes of leaf thickness. Greater relative water content of leaves and lower osmotic potential of sap expressed from turgid leaves were recorded in the severe water deficit treatment than in the weak water deficit treatment. This finding also postulated physiological adaptation to be better water status under drought stress. The drought imposition affected significantly on photosynthesis, water use efficiency and stomatal conductance of tomato plants. The severe water-deficit treatment increased PSII activities and water use efficiency, but decreased stomatal conductance than the weak water-deficit treatment. However, there were no differences between the two treatments in total photosynthetic capacity. Finally, there were no differences in the number and biomass of fruits. These results suggested that tomato plants have an ability to make adaptation to water deficit conditions through changes in leaf morphology, osmotic potentials, and water use efficiency as well as PSII activity. These adaptation responses should be considered in the screening of drought tolerance of tomato plants.
Choi, Gyeong Lee;Yeo, Kyung Hwan;Choi, Su Hyun;Jeong, Ho Jeong;Kang, Nam Jun;Choi, Hyo Gil
Journal of Bio-Environment Control
/
v.26
no.4
/
pp.418-423
/
2017
In hydroponics, the nutrient solution is supplied considering the water and nutrient uptake characteristics of crops. However, as the ionic uptake characteristics are changed as a result of the weather conditions or the growth response of the crops, the root zone can not be maintained in optimal condition. In addition, the coir substrate has been used mainly for the tomato cultivation in place of the inorganic substrate, there are few studies on long-term cultivation using coir substrate. Therefore, this study was conducted to investigate the effect of EC level of irrigation solution on tomato growth and inorganic ions of root zone in soilless culture using coir. Coir substrate mixed with 5 : 5 chip and dust was used. EC level of irrigation solution was 1.0, 1.5, 2.0, and $3.0dS{\cdot}m^{-1}$. At the initial stage, $NO_3-N$, P, Ca and Mg in the drainage were lower than the irrigation level at 1.0 and $1.5dS{\cdot}m^{-1}$. However, EC $2.0dS{\cdot}m^{-1}$ or higher, all the ions except P were highly concentrated in the drainage. The average fruit weight was not significantly different between 1.0 and $1.5dS{\cdot}m^{-1}$ until 3th cluster, but from the next cluster, the higher the EC level, the smaller the weight. The number of fruit and yield to 6th cluster was the highest at $1.5dS{\cdot}m^{-1}$. From the next cluster, The yield was decreased with the higher EC level. At the early stage of growth, BER occurred only in EC $3.0dS{\cdot}m^{-1}$, but increased in all treatments with increasing irradiation. The incidence rate of EC $3.0dS{\cdot}m^{-1}$ was higher than that of the lower EC level treatment.
The purpose of this study was to investigate the extraction yield and quality stability as to the oleoresin process with large amount of onion at one time. The first mixed-product is raw onion juice which was reduced the compression and concentrated by Brix 70% mixed together wit the residue which was extracted and concentrated by ethanol, the second product manufactured by the same method above after the autoclaving with onion, and the other product is made by grinding by 50mesh to freeze-dried onion. Each of yields were 7.3, 9.1 and 0.8% and each of total sugar content was 616.4, 712.3 and 150.3mg/g. Therefore the product extracted by ethanol from freeze-dried onion was very low in yield and total sugar content. By the index of the overall odor intensity, contents of total pyruvate were 1,733.7, 520.6, and 2,716.5$\mu\textrm{g}$/g for each product. As a result, oleoresin onion processing that desired to use raw onion was remarkable for odor recovery. For the homogenous mixture with concentrate of onion juice and ethanol extract were emulsified by the addition of 2% of PGDR(polyglycerol condensed ricinoleate) and agitation(10,000rpm, 30 minutes). At this time, interfacial tension was 1.9 dyne/cm and the formation of emulsion was for 96.2% when left over 24hours in 6$0^{\circ}C$. When it was to be centrifuged(2,000$\times$G, 80 minutes) after emulsification, the volume of emulsion level without seperation was 92.6%, and very high in emulsification stability. The induced heating-oxidize with soy bean oil and sesame oil added to 1% of onion oleoresin, induction-time extension effect appeared with antioxidant activity that was applicable for 80.8~82.2% as to the effect of addition of 0.02% BHA.
Park, Hong-Kyu;Kim, Sang-Su;Choi, Won-Yong;Lee, Ki-Sang;Lee, Jae-Kil
KOREAN JOURNAL OF CROP SCIENCE
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v.47
no.3
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pp.167-173
/
2002
This experiment was conducted to figure out the change of soil physical properties, rice growth and yield with the years of continuous cultivation in direct dry-seeding and no-tillage machine transplanting. Experiments were conducted at NHAES(National Honam Agricultural Experiment Station, RDA, Iksan, Cheon Buk Province, South Korea) with a rice variety "Dongjinbyeo" from 1995 to 2000. In no-tillage machine transplanting cultivation, organic matter in soil was higher than that on direct dry-seeding and was significantly high in topsoil. Problematic weed species were E. crus-galli B., A. keisak H., and L. japonica M. Plant height and tiller number m-2 were higher in common-tillage during the total growth duration. The highest weedy rice occurrence of 27.5% was observed in live years' continuous direct dry-seeding and followed by 6.2%, in four years', and 3.7%, in three years'. The highest yield reduction of 38% was observed in five years' continuous direct dry-seeding. The reduction may resulted from the competition between weedy rice and cultivated rice.
Lee, Jundae;Park, Seok Jin;Do, Jae Wahng;Han, Jung-Heon;Choi, Doil;Yoon, Jae Bok
Horticultural Science & Technology
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v.31
no.4
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pp.473-482
/
2013
Molecular markers, as an efficient selection tool, have been and is being used for practical breeding program in chili pepper (Capsicum annuum L.). Recently, a lot of researches on inheritance and genetic analysis for quantitative traits including capsaicinoids, carotenoids, and sugar content in pepper are being performed worldwide. It has been also reported that QTL mapping is a necessary tool to develop molecular markers associated with the quantitative traits. In this study, we suggested a new method to construct a pepper genetic map using SNP (HRM) markers generated from NGS resequencing of female and male parents. Plant materials were C. annuum 'NB1' (female parent), C. chinense 'Jolokia' (male parent), and their $F_2$ population consisting of 94 progenies. Sequences of 4.6 Gbp and 6.2 Gbp were obtained from NGS resequencing of 'NB1' and 'Jolokia', respectively. Totally, 4.29 million SNPs between 'NB1' and 'Jolokia' were detected and the 1.76 million SNPs were clearly identified. Among them, total 145 SNP (HRM) primer pairs covering pepper genetic map were selected, and the 116 SNP (HRM) markers of them were located on this map. Total distance of the map, which consisted of 12 linkage groups and matched with basic chromosome numbers of pepper, was 1,167.9 cM. According to the mapping result, we concluded that our mapping method was suitable to construct a pepper genetic map fast and accurately. In addition, the genetic map could be directly used for QTL analysis of traits different between both parents.
Kim, JunHyeok;Park, YoSup;Kwon, YongHee;Jung, MyungHee;Park, Hee-Seung
Horticultural Science & Technology
/
v.35
no.2
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pp.170-177
/
2017
This study was conducted to estimate the light pollution damage caused by night-break lighting in 'Kyoho' grapes. Night irradiation was performed every night for four hours (10 p.m. to 2 a.m.) from the full bloom to the harvest stage using red, blue, and white lamps as artificial light. Fruit yield, soluble solid content, anthocyanin content, and chlorophyll a content were affected by night irradiation. The soluble solid content of the control was $17.5^{\circ}Brix$, approximately $1^{\circ}Brix$ higher than the red ($16.4^{\circ}Brix$), blue ($16.2^{\circ}Brix$), and white light treated grapes ($16.3^{\circ}Brix$). The anthocyanin content of the skin was also higher in the control at $4.08{\mu}g{\cdot}cm^{-2}$ compared to the red ($3.14{\mu}g{\cdot}cm^{-2}$), blue ($2.47{\mu}g{\cdot}cm^{-2}$), and white ($2.82{\mu}g{\cdot}cm^{-2}$) light treated samples. On the other hand, the chlorophyll a content of the control was the lowest at $0.268{\mu}g{\cdot}cm^{-2}$ as compared with the red ($0.339{\mu}g{\cdot}cm^{-2}$), blue ($0.345{\mu}g{\cdot}cm^{-2}$), and white ($0.372{\mu}g{\cdot}cm^{-2}$) light treated samples. Considering that higher soluble solid contents, higher accumulation of anthocyanin, and lower chlorophyll a contents are factors involved in fruit maturation, night irradiation may delay fruit maturation and red light treatment may result in decreased yield. Our results confirmed that night-break lighting regardless of the wavelength provoked light pollution in 'Kyoho' grapes. Therefore, fruit maturation may be poor in the presence of artificial light, including streetlamps, in neighboring vineyards.
The purpose of this study was to evaluate the filtering-feeding effect of a freshwater mussel (Corbicula leana) on the phytoplankton communities in two lakes with different trophic conditions between June and September, 2000. Manipulation experiments were conducted with two treatments (the control and mussel addition), and each established in duplicate 10 l chambers. Both ambient nutrient (TN, TP) and chlorophyll-a concentrations were significantly (p<0.01) higher in Lake Ilgam than Lake Soyang. Cyanophytes (Microcystis, Oscillatoria, Lyngbya and Dactylococcopis) consistently dominated algal community in Lake llgam, while flagellated algae (Dinobryon divergence, Mallomonas, Rhodomonas) and cyanophytes (Microcystis)dominated in Lake Soyang. The net exponential death rate ($R\;=\;day^{-1}$) of total phytoplankton in the mussel treatment ranged $1.70{\sim}7.39$ and $0.38{\sim}1.64$ in Lakes Soyang and Ilgam, respectively. Mean filtering rate standardized by mussel AFDW ($ml\;mgAFDW^{1}\;h^{-1}$) was much higher in Lake Soyang ($1.70{\sim}3.06$) than in Lake Ilgam($0.24{\sim}0.88$0.24~o.88). Estimating FR per mussel, 1 mussel filtered $1.6{\sim}7.8\;l$ per day and $1.7{\sim}3.0\;l$ per day in Lakes Soyang and Ilgam, respectively. Based on tile C-flux tobiomass ratio, Corbicula leana consumed $0.8{\sim}4.4$ fold of phytoplankton standing stock in Lake Soyang, and $0.4{\sim}1.6$ fold in Lake Ilgam per day. Mussel feeding resulted in increase of SRP concentration by $30{\sim}50%$, compared with the control. The results of this study suggest that filter-feeding activity of Corbicula leana varies depending on the phytoplankton density and community composition. The high seston consumption rate of Corsicuja Jeaua even in a eutrophic lake suggests that biomanipulation approach using filter-feeding mussels can be used far wate rquality management in small eutrophic reservoirs.
Reusing livestock manure have various advantages in securing soil organic resources. In this study, the N-loading and recycling capacity assessment of animal manure was analyzed by comparing between the cultivated areas of crops and the amount of manure units that are generated from livestock manure. From this assessment, the possibility of recycling resources of livestock manure was evaluated. The amount generated of livestock manure in Gyeonggi-do were evaluated by applying the manure units to the number of livestock manure. The analysis of manure unit per ha shows that the N loading by MU is quite different by region. When it comes to nitrogen loading, the MU per ha of cultivated land in excess of the N-amount was the highest in the Gyeonggi-do province with 2.70 MU/ha, which is higher than the appropriate level. The Chungcheongnam-do province came next with 2.31 MU. So the recycling capacity assessment was carried out mainly based on areas of forage crops, rice that can be provided by recycling livestock manure. The recycling capacity ware highest in Jeollanam-do and Jeollabuk-do. In order to properly apply the livestock manure into organic resources, the seasonal situation that effects the nitrogen demands of crops along with the regional effects crop cultivation should be seriously analyzed. In addition, a system that can effectively produce and manage fertilizer should be established.
There are around 7,500 manure tanks to treat the manures from pigs in Korea. In the tank, there are too much sediments deposited on the base and wall, which causes low efficiency of stock capacity and manure fermentation. In order to minimize sediments and to ferment manure effectively, we developed a 2-fluid jet mixer for mixing sediments in liquid livestock manure tank. For developing the prototype, we tested a factorial experimental system with various nozzles, and simulated CFD models with two kinds of nozzle arrangement. From the results of factorial experiment and CFD simulation, we concluded the dia. ratio of primary : secondary nozzle should be 1:2 and the nozzles should be arranged at the same distances toward to the circumferential direction. With this results, we manufactured a 2-fluid jet mixer which is consists of four 2-phase nozzles, centrifugal slurry pump and root's type air blower. And, we carried out the performance test of the prototype in the round shaped liquid manure tank in the farm. The performance test results showed that the uniformity of TS (Total Solid) and VS (Volatile Solid) was raised from 21.3 g/L, 13.3 g/L In steady state to TS and VS to 23.0 g/L, 14.1 g/L in the mixing operation. Therefore, we could conclude that the prototype of 2-fluid mixer could make the solid material which could be sediments in the tank not to be deposited in the tank and to be contacted to air bubbles which could enhance the efficiency of the fermentation of livestock manure.
Purpose: Presently, any exact standard of radiopharmaceutical doses in pediatric nuclear medicine doesn't exist in the universe. So hospitals are following by manual of vial kit or guidelines of America and Europe based on recommended adult doses adjusted for body mass (MBq/kg) or body surface area (MBq/$m^2$). However, especially for children younger than 1 year and heavier than 50 kg, it's hard to estimate exact dosage for those children. Materials and Methods: In order to obtain objective data of multipliers for pediatric studies, we surveyed 4 major hospitals in Korea. After receiving feedbacks, we changed dosage to multiplier. And we compared multipliers of Korea to America's and Europe's. Results: Most hospitals in Korea are following by body mass formula (MBq/kg). On the other hand, standards don't include proper factors for a child younger than 1 year and heavier than 50 kg. Multipliers for 3 kg children who are injected lower doses than needed are America:0.12, Europe:0.09, Korea:0.05, multipliers for 30 kg children who are injected proper doses are America:0.58, Europe:0.51, Korea:0.45 and multipliers for 60 kg children who are injected more doses than needed are America:0.95, Europe:0.95, Korea:0.91. Conclusions : Through the survey, when calculating doses for children, usually output doses are based on adult doses adjusted for body mass (MBq/kg) but research has shown that standards of all of the compared standards don't reflect exact multipliers for children younger than 1 year and heavier than 50 kg. Therefore, we should give an effort to reduce needless radiation exposure in children by establishing a proper doses standard and also developing better image reconstruction software.
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