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Vitellogenin ELISA System Based on Monoclonal and Polyclonal Antibodies against Vitellin of Floating Goby (Chaenogobius annularis) (꾹저구(Chaenogobius annularis)의 난황단백질에 대한 다클론 항체와 단글론 항체을 이용한 Vitellogenin ELISA System)

  • Kang, Bong-Jung;Jung, Jee-Hyun;Lee, Je-Yong;Kim, Myung-Hee;Han, Chang-Hee
    • Development and Reproduction
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    • v.9 no.2
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    • pp.135-142
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    • 2005
  • Vitellogenins(VTGs) are the precursor of egg-yolk proteins in most oviparous species from invertebrates to vertebrates. In oviparose vertebrates, VTGs are synthesized in the liver and transported through the blood to oocytes. In female fish, concentrations of plasma VTG increase rapidly at onset of vitellogenesis in the normal reproductive cycle. Male fishes also possess the gene for VTG, but plasma concentrations of the protein typically remain small, presumably due to low levels of endogenous estrogens. However, exposure of males to exogenous estrogenic mimics can result elevated. Therefore, the VTG in fish can be used as a useful biomarker for appropriate tools of endocrine disrupting compounds effects. In this studies, we prepared the test methods that can measure the plasma VTG level in the gobies that live in polluted area with mimic estrogen. For the purpose, we purified VTG of floating goby(Chaenogobius annularis) and prepared specific monoclonal and polyclonal antisera to yolk protein, then developed a sandwich competitive ELISA system for measurement of plasma VTG levels. Validation for the ELISA system using monoclonal and polyclonal antibodies against VTG was tested. The absorbance curve of serial dilutions of serum from vitellogenic female was paralleled to the standard curve of VTG, but normal male was not paralleled. The developed sandwich ELISA system was measured for VTG levels in plasma of common goby(Acanthogobius flaviman) and javeline goby(A. hasta) as well as in plasma of floating goby(C. annularis).

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The Interaction Index Between Grasses and Weeds in the Grassland Composed with Mainly Perennial Ryegrass (페레니얼 라이그라스 위주 초지에서 목초, 잡초 식생 군락간 상호작용)

  • Lim Keun-Bal;Sung Byung-Ryeol;Lee Hyun-Jun;Ahn Byung-Suk;Seo Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.2
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    • pp.105-112
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    • 2006
  • This experiment was carried out to investigate the distribution, function of dry matter and competition in the grassland composed with mainly perennial ryegrass. Co-functions between grasses and weeds were evaluated by calculating methods; expected yield (P), agressivity, relative yield total (RYT), compensation index (CI) and morphological index (MI). Each values obtained were influenced by seeding method, grassland management and seasonal changes of co-functional index were identical. Expected yields of spring were lower than those of fall and improved by additional seeding to grassland in early spring time. In the relative yield total (RYT), all were under 1.0 which ranged from 0.17 to 0.41. Compensation index (CI) ranged from -0.3 to -0.6 and all values showed 'under compensation(+, --)' This means that the conditions of 'under compensation' could be improved through weed control by management of grassland in perennial ryegrass grassland. The results indicated that productivity of grassland mainly composed with perennial ryegrass is negatively affected by weeds like shepherd's purse, crabgrass and barnyard grass. As major weeds, shepherd's purse in spring, crabgrass in summer and barnyard grass in summer and fall were negatively functioned to productivity in the grassland composed with mainly perennial ryegrass.

Effect of Herbicide 'Kerb' on Growth of Perennial Ryegrass (Lolium perenne L. 'Accent') and Zoysiagrass (Zoysia japonca 'Zenith') under Shading Treatments (차광조절하에서 제초제 Kerb가 퍼레니얼 라이그래스와 한국잔디의 생육에 미치는 영향)

  • Ham, Tae-Hwan;Yun, Jong-Jin;Lee, Sang-Gi;Ku, Ja-Hyung
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.52-58
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    • 2011
  • In order to investigate an artificially transition method of perennial ryegrass(Lolium perenne L. 'Accent') overseeded on zoysiagrass (Zoysia japonca Zenith') with Kerb (3,5-jichloro-N (1,1-dimethyl-2-propynyl). We observed turfgrass growth and physiological responses of the two turfgrasses under the shading treatments. The experiment was conducted in greenhouse during April- August. Shading was generally using a black shade net product whose the shade level was done into 0, 35 and 75%, actual light intensities were 589, 378 and $135\;{\mu}mol{\cdot}m^{-2}{\cdot}s$ PAR, respectively. The four levels of Kerb treatment were 0, 0.1 0.2, and $0.4\;g{\cdot}m^{-2}$. As shading was getting increased, growth of perennial ryegrass was increased slowly, but zoysiagrass was reduced and almost all of experiments showed significant differences statistically. Perennial ryegrass exhibited inclination that growth was reduced in more than Kerb $0.4\;g{\cdot}m^{-2}$ by shades. On the other hand, it was shown that the growth of zoysigrass was reduced by shades largely and it was not effected by Kerb. Through this study, it was determined that Kerb is effective for elimination of overseeded perennial ryegrass on zoysiagrass. Moreover, when excluding perennial ryegrass under the condition like shade, it is desirable for treating high concentration more than the basic processing amount.

The Change of Geographical Names' Territory and Representation of Place Identity with Place Names : A Case Study of Chungju Geographical Names (지명을 통한 장소정체성 재현과 지명영역의 변화 : 충주지역 지명을 사례로)

  • Lee, Young-Hee
    • Journal of the Korean association of regional geographers
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    • v.16 no.2
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    • pp.110-122
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    • 2010
  • This article is to study the characteristics of Chungju geographical names using the concepts which are the place identity, the politics of scale, and the competition of place names' territory for the diverse study methods of geographical names. According to this results, the new name of 'Suanbo-Myun' revealed the place identity, because it has not only the property of 'Suanbo hot spring' but also the three conditions called 'the numerical solitary', 'the qualitative identity', and 'the self-identity' which are the conditions for the place identity. In relation to the politics of scale through place names, the example of scaling up is 'Yian-Myun' which is former name of 'Chungju city Yiru-Myun', and the cases of scaling down are 'the up and down of Chungju Up Ho', the reductional change from 'Chungju-Gun' to 'Chungju-Myun' and the change of Chinese name of the 'Wolak Mountain'. Lastly, the examples of place names' territory change are two types. One is 'Chungju Yongdu-Dong' and 'Yiru-Myune Geumgok-Ri' for the cases that the place name and its territory were changed. The other is 'Dalchon river' that the place name's territory was only changed. In conclusion, this study suggested that place names are useful in order to represent and construct the place identity.

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Handoff Improvement Method of an AP Choice for Guarantee of Mobile Node Network Performance according to Speed in Wireless LAN System (무선랜 시스템에서 속도에 따른 이동노드 네트워크 성능 보장을 위한 AP 선택 핸드오프 개선기법)

  • Kim, Dong-Geun;Park, Jae-Heung;Kim, Sang-Bok
    • The Journal of the Korea Contents Association
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    • v.10 no.6
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    • pp.64-71
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    • 2010
  • When Handoff occurs at wireless network, existing AP search chooses one of various APs according to signal strength information. However, IEEE 802.11 uses Medium Access method with CSMA/CA that competes to obtain a channel by sharing medium. Therefore, network performance is heavily affected by the number of nodes and network congestion aside from signal strength. This scheme presented an additional AP selection indicator and a new handoff algorithm to realize handoff guaranteeing the network performance of mobile node in a process selecting new AP. This indicator is includes a handoff cost function reflecting the network information of mobile node, and the mobile node made handoff guaranteeing the network performance using network information possible by using this. also we classified the nodes into high-speed node and low-speed node. Then, when Handoff occurs, high-speed node was made itself choose AP by signal strength information. We also made the low-speed node choose its AP by using AP network information which is applied area. Hence, we demonstrate through simulation that gets an improvement in performance, even in the wireless network which many users concurrently access to, and it has considerable effects on aspects of resources and network management by distribution of users.

Some Seaweed Deseases Occurred at Seaweed Farms along the South-Eastern Coast of Korea (동해남부연안 미역양식장의 병충해)

  • KANG Jae-Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.165-170
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    • 1981
  • Recently an unknown microbial desease and some parasitic crustaceans are prevailing in the sea-weed farms of Uudaria pinnatifida along the south-eastern coast of Korea.(1) Green spots probably caused by the microbial pathogens were found on the fronds of Undaira Pinnatifide. Particularly they were densely distributed on the distal half of the fronds. The tissues of the green spot area rot off, and small holes with green colored margin are formed. The holes at the distal part of the fronds are enlarged and they finally coalesced each other. Then this process accelerates decaying of the distal ends of the fronds.. The fronds growing in the central part of the farms are usually severely damaged, whereas in the marginal area of the farm toward the open sea side the damage is less serious. An examination revealed that the deseased fronds bore a number of viable bacteria, $6.8\times10^5\;to\;1.2\times10^6$ per gram at $15^{\circ}C$, whereas the healthy fronds $1.1\times10^4$. Twenty-six kinds of colonies, 247 strains of bacteria, were isolated from deseased fronds, belonging to Moraxella, Achromobacter, Vibrio, Flavobacterium, Acinetobacter, Pseudomonas, etc. (2) Pinholes occurred in one series on the frond. They were probably caused by a harpacticoid copepod, Thalestris sp. Seven years ago when the disease was first found to occur the copepod was observed on the fronds from March of the year. Recently, however, they have been found as early as December of the previous year. (3) A gammarid amphipod, Ceinina iaponica, invades the pith of the midrib through holdfast of thalli. This rarely causes the longitudinal seperation of the entire frond through the midrib as they bore a tunnel in the pith. Sometimes holdfasts of tile heavy damaged thalli make the frond departed from the substrate.

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Sprouting and Emergence Properties of Eleocharis kuroguwai Ohwi (올방개 괴경(塊莖)의 맹아(萌芽) 및 출현(出現) 특성(特性))

  • Lee, H.K.;Lee, I.Y.;Ryu, G.H.;Lee, J.O.;Lee, E.J.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.233-238
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    • 1994
  • The experiment on sprouting and emergence properties of Eleocharis kuroguwai was conducted at laboratory and greenhouse conditions in 1993. Most of tubers had the apical dominance of buds at sprouting and had the competitive relationship for mesocotyl elongation. When pyrazosulfuron was applied on soil surface, all of apical buds were killed, but the first and second lateral buds survived to 50% and 90%, respectively, and the third lateral buds were almost at presprouted state. The tubers at 10-15cm deep soil emerged 1 week late and 35% low in emergence rate compared with the tubers at 2-5cm deep. As the plants emerged from the deep soil, the mesocotyl and the roots were distributed at deep soil more or less. Among the tubers buried at 10cm and 15cm deep soil, 25% and 30% respectively were not emergerd due to the suspension of mesocotyl elongation. Although the roots and shoots were removed from the tubers at the stage of 20cm high, all of mother tubers emerged again even 2 weeks late.

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Change in Weed Problems as Influenced by Transition of Cultural Pattern into Direct Seeding (직파재배(直播栽培)로의 양식(樣式) 전환(轉換)에 따른 논 잡초문제(雜草問題)의 변화(變化))

  • Guh, Ja-Ock;Im, Il-Bin
    • Korean Journal of Weed Science
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    • v.12 no.3
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    • pp.223-229
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    • 1992
  • Very recently, the cultural pattern of paddy rice in Korea was transitted in a short period, and resulted as earlier in season and more in fertillizer application among others. Specially the weed emergence in paddy fields was drastically increased as affected by change of cultural pattern into early planting and into direct seeding methods. Of course, the direct-seeding in row seeding induced more weeds than in broad-cast direct -seeding. By maintaining the water level with saturation of the paddy surface induced more weeds, relatively more annual weeds than by deep in water level, respectively. Relatively more annuals in number of species and in emerged quantity comparing with perennials was caused by the shifting of cultural methods from transplanting to direct seeding. The severe weed competition damages were mainly resulted due to the shading by taller plant heights of weeds than crop during the whole growing seasons. Conclusively, accepting of direct seeding methods in paddy rice culture in Korea, the present bases for weed control methods ought to be naturally and effectively improved to meet the newly made weed problems.

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The Discursive Topography in Maker Culture A Critical Discourse Analysis of 'Maker Movement' (메이커 문화를 둘러싼 담론적 지형 메이커 운동(maker movement)에 대한 비판적 담론 분석)

  • Choi, Hyuk Kyoo
    • Korean journal of communication and information
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    • v.82
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    • pp.73-103
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    • 2017
  • With the introduction and expansion of 'maker movement', maker culture captured attention and saw itself as an emerging culture. This study aims to analyze published books, policy report, columns and news articles related to maker culture through the perspective of critical discourse analysis. Maker movement led by the government gives meaning to the maker culture as the force of 'creative economy' that can overcome the economic crisis. Following this meaning making, one-man digital fabrication start-ups have been actively promoted by government policies. In the case of Seoul, it criticizes government led maker movement that only focuses on economy and institutionalizes maker movement by focusing on the maker culture's aspect as 'digital social innovation' that can resolve social problems. In the world of art, it tries to rediscover the value craft, that is, 'creative craftsman'. Moreover, resistance movement that tries to fight against dominant technology structure through constructing 'critical making' was also spotted. Nonetheless, it is rather untimely to definitely find dominant discourse's power effect in reality and sign of rupture in dominant structure as the result of resisting discourse's struggle. Thus, maker movement is the field of struggle where an ongoing clash can be found: between discourse strategy that tries to make maker culture a social or economic asset by combining with dominant power structure, and alternating or resisting practice of signification that focuses on its cultural techno-political potential.

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An Exploration on Personal Information Regulation Factors and Data Combination Factors Affecting Big Data Utilization (빅데이터 활용에 영향을 미치는 개인정보 규제요인과 데이터 결합요인의 탐색)

  • Kim, Sang-Gwang;Kim, Sun-Kyung
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.30 no.2
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    • pp.287-304
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    • 2020
  • There have been a number of legal & policy studies on the affecting factors of big data utilization, but empirical research on the composition factors of personal information regulation or data combination, which acts as a constraint, has been hardly done due to the lack of relevant statistics. Therefore, this study empirically explores the priority of personal information regulation factors and data combination factors that influence big data utilization through Delphi Analysis. As a result of Delphi analysis, personal information regulation factors include in order of the introduction of pseudonymous information, evidence clarity of personal information de-identification, clarity of data combination regulation, clarity of personal information definition, ease of personal information consent, integration of personal information supervisory authority, consistency among personal information protection acts, adequacy punishment intensity in case of violation of law, and proper penalty level when comparing EU GDPR. Next, data combination factors were examined in order of de-identification of data combination, standardization of combined data, responsibility of data combination, type of data combination institute, data combination experience, and technical value of data combination. These findings provide implications for which policy tasks should be prioritized when designing personal information regulations and data combination policies to utilize big data.