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Development of cordycepin fortified milk production in Holstein cows I. Effects of various levels of Cordyceps militaris mycelia from grains supplement on cordycepin content in milk in dairy cows (Cordycepin 강화 우유 생산에 관한 연구 I. 동충하초 균사체의 적정사용량 결정을 위한 사양연구)

  • Yeo, J.M.;Lee, S.H.;Kim, D.H.;Hwang, J.H.;Kim, W.Y.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.103-111
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    • 2009
  • This study was carried out to determine effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows. Ten Holstein cows in the early stages of lactation were divided into two groups. Control group received no supplement whereas treatment group received 6% of C. militaris mycelia of their dry matter intake for 6 months. Feed intake, milk urea nitrogen and somatic cell counts were not affected by long-term supply of C. militaris mycelia for the whole period. In addition, milk yield and milk composition were not affected by long-term supply of C. militaris mycelia at any time of the periods with the exception of milk protein content and yield. The average of milk protein content and yield from the whole period was higher for C. militaris mycelia supplement group than for the control group. As expected, cordycepin in whole blood and milk was not detected in the control group. The range of cordycepin content in the treatment was 0.31~0.38µ/ml and 0.18~0.26(µ/ml for whole blood and milk, respectively. Individual variation was found to be very high and, furthermore cordycepin was undetected in some milk samples. Thus, no clear pattern could be seen in cordycepin content in milk throughout the whole period. Overall, the results of the present study suggest that the transfer efficiency of cordycepin to milk by supplementing C. militaris mycelia in dairy cows was unpredictable and low.

Development of cordycepin fortified milk production in Holstein cows II. Effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows (Cordycepin 강화 우유 생산에 관한 연구 II. 장기간 동충하초 균사체급여가 우유 중의 cordycepin 함량 변화에 미치는 영향)

  • Yeo, J.M.;Lee, S.H.;Kim, D.H.;Hwang, J.H.;Kim, W.Y.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.113-123
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    • 2009
  • This study was carried out to determine effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows. Ten Holstein cows in the early stages of lactation were divided into two groups. Control group received no supplement whereas treatment group received 6% of C. militaris mycelia of their dry matter intake for 6 months. Feed intake, milk urea nitrogen and somatic cell counts were not affected by long-term supply of C. militaris mycelia for the whole period. In addition, milk yield and milk composition were not affected by long-term supply of C. militaris mycelia at any time of the periods with the exception of milk protein content and yield. The average of milk protein content and yield from the whole period was higher for C. militaris mycelia supplement group than for the control group. As expected, cordycepin in whole blood and milk was not detected in the control group. The range of cordycepin content in the treatment was 0.31~0.38µ/ml and 0.18~0.26(µ/ml for whole blood and milk, respectively. Individual variation was found to be very high and, furthermore cordycepin was undetected in some milk samples. Thus, no clear pattern could be seen in cordycepin content in milk throughout the whole period. Overall, the results of the present study suggest that the transfer efficiency of cordycepin to milk by supplementing C. militaris mycelia in dairy cows was unpredictable and low.

Development of cordycepin fortified meat production in Hanwoo steers I. Determination of the chemical composition and safety of Cordyceps militaris from grain or pupae and Paecilomyces tenuipes (Cordycepin 강화 한우고기 생산에 관한 연구 I. 곡립기주 동충하초 균사체의 화학적 성분 및 안전성 조사)

  • Kim, W.Y.;Lee, S.H.;Kim, D.H.;Lee, J.H.;Nho, W.G.;Hwang, J.H.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.35-51
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    • 2009
  • Three different Cordyceps militaris(Paecilomyces tenuipes and Cordyceps militaris from grain or pupae) were analysed for their chemical composition, fatty acids composition and the concentration of amino acids, cordycepin and heavy metal to obtain basic information on them as feed supplements for livestock. The crude protein content of C. militaris from grain and pupae was 76.16 and 75.45%, respectively, being higher than that of P. tenuipes(57.21%). The concentration of linoleic acid was much higher but that of linolenic acid was much lower for C. militaris from grain than for the others. The cordycepin content was significantly higher for C. militaris from grain than for that from pupae (1.64% vs 0.68% on a DM basis). But cordycepin was not detected in P. tenuipes. Heavy metal contents(Cr, Pb, Cd, Hg) for all C. militaris were below the allowance levels recommended by Ministry of Agriculture and Forestry in Republic of Korea.

Studies on the MSM (Methyl Sulfonyl Methane) Treatment Method of Soybeans (콩의 MSM (Methyl Sulfonyl Methane) 처리 방법에 관한 연구)

  • Se Eun Chae;Seung Ka Oh;Young-Son Cho;Doobo Shim;Dong-Kyung Yoon;Seung Ho Jeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.69 no.1
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    • pp.25-33
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    • 2024
  • This experiment was conducted at the affiliated farm of the Suncheon University from 2022 to 2023 to investigate the growth, yield, and quality characteristics of soybeans based on the treatment method of Methyl Sulfony Methane (MSM) for the establishment of stable production practices. In the initial investigation of growth characteristics in 2022, an increasing trend in characteristics such as plant height, stem thickness, and branching index was observed as the treatment concentration increased from 50% to 200%. Yield components also followed the same trend, with the basal fertilization + top dressing 3 times at 200% treatment showing the highest yield at 355 kg·10a-1, with the highest number of pods. In the subsequent study to determine the optimal concentration exceeding 200% in 2023, growth characteristics showed a trend of 400% > 200% > 800%. The basal fertilization + top dressing 3 times at 400% treatment exhibited the longest plant height (106.7 cm) and the most branches (6). In terms of seed quality, this treatment also had the highest proportion (66.9%) of seeds with a diameter over 6.7 mm. Additionally, in yield components such as pods, seeds per pod, and 100-seed weight, the basal fertilization + top dressing 3 times at 400% treatment showed the highest values, resulting in a maximum yield of 374 kg·10a-1, representing a 23.4% increase compared to the control. Therefore, for the optimal production of high-quality soybeans, it is recommended to apply the treatment of basal fertilization + top dressing 3 times at 400% concentration, with top dressing occurring at 30-day intervals before harvest.

Adaptability of the high first pod height, shattering-resistant soybean cultivar 'Saegeum' to mechanized harvesting (고착협 내탈립 기계수확 적응 장류·두부용 콩 품종 '새금')

  • Kim, Hyun Tae;Han, Won Young;Lee, Byung Won;Ko, Jong Min;Lee, Yeong Hoon;Baek, In Youl;Yun, Hong Tai;Ha, Tae Joung;Choi, Man Soo;Kang, Beom Kyu;Kim, Hyun Yeong;Seo, Jeong Hyun;Kim, Hong Sik;Shin, Sang Ouk;Oh, Jae Hyun;Kwak, Do Yeon;Seo, Min Jeong;Song, Yoon Ho;Jang, Eun Kyu;Yun, Geon Sik;Kang, Yeong Sik;Lee, Ji Yun;Shin, Jeong Ho;Choi, Kyu Hwan;Kim, Dong Kwan;Yang, Woo Sam
    • Korean Journal of Breeding Science
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    • v.51 no.4
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    • pp.496-503
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    • 2019
  • The soybean cultivar, 'Saegeum', has been developed for preparing soy-paste and tofu. The soybean cultivars 'Daepung' and 'SS98207-3SSD-168' were crossed in 2003 to obtain 'Saegeum'. Single seed descent method was used to advance the generation from F3 to F5, and the plant lines with promising traits were selected from F6 to F7 by pedigree method. The preliminary yield (PYT) and advanced yield trials (AYT) were conducted from 2009 to 2010, and the regional yield trial (RYT) was conducted in 12 regions between 2011 and 2013. The morphological characteristics of 'Saegeum' were as follows: determinate plant type, white flower, tawny pubescence color, and brown pod color. Flowering and maturity dates were August 2, XXXX and October 17, XXXX, respectively. Plant height, first pod height, number of nodes, number of branches, and number of pods were 79 cm, 18 cm, 16, 2.3, and 44, respectively. The seed characteristics of 'Saegeum' were as follows: yellow spherical shape, yellow hilum, and the 100-seed weight was 25.4 g. 'Saegeum' was resistant to bacterial pustule and SMV in the field test, and its lodging resistance was mildly strong, whereas its shattering resistance was excellent. The ability of this cultivar to be processed into tofu, soybean malt, and other fermented products was comparable with that of 'Daewonkong'. The yield of 'Saegeum' in the adaptable regions was 3.02 ton ha-1. Thus, 'Saegeum' is adaptable to mechanized harvesting because of its high first pod height, as well as lodging and shattering resistance. (Registration number: 5929)

Simulation of Groundwater Flow and Sensitivity Analysis for a Riverbank Filtration Site in Koryeong, Korea (경북 고령군 강변여과 취수 지역의 지하수 유동 모사 및 민감도 분석)

  • Won, Lee-Jung;Koo, Min-Ho;Kim, Hyoung-Su
    • Journal of Soil and Groundwater Environment
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    • v.11 no.2
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    • pp.45-55
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    • 2006
  • A 2-D unconfined flow model is developed to analyze annual variations of groundwater level and bank filtration rate (BFR) for an experimental riverbank filtration site in Koryeong, Korea. Two types of boundary conditions are examined for the river boundary in the conceptual model: the static head condition that uses the average water level of the river and the dynamic cyclic condition that incorporates annual fluctuation of water level. Simulations show that the estimated BFR ranges $74.3{\sim}87.0%$ annually with the mean of 82.4% for the static head boundary condition and $52.7{\sim}98.1%$ with the mean of 78.5% for the dynamic cyclic condition. The results illustrate that the dynamic cyclic condition should be used for accurate evaluation of BFR. Simulations also show that increase of the distance between the river and the pumping wells slightly decreases BFR up to 4%, and thereby indicate that it is not a critical factor to be accounted for in designing BFR of the bank filtration system. A sensitivity analysis is performed to examine the effects of model parameters such as hydraulic conductivity and specific yield of the aquifer, recharge rate, and pumping rate. The results demonstrate that the average groundwater level and BFR are most sensitive to both the pumping rate and the recharge rate, while the water level of the pumping wells is sensitive to the hydraulic conductivity and the pumping rate.

The Early Life History of the Rockfish, Sebastes inermis -2. Morphological and Skeletal Development of Larvae and Juveniles- (볼락, Sebastes inermis의 초기생활사에 관한 연구 -2. 산출 자치어의 외부형태 및 골격발달-)

  • KIM Yong-Uk;HAN Kyeong-Ho;BYUN Sun-kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.5
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    • pp.465-476
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    • 1993
  • Rockfish, Sebastes inermis is a ovoviviparous fish distributed around the coastal waters of Korea and Japan. Naturally beared larvae obtained from female fish kept in the aquarium were reared for three months in the laboratory. The naturally healed larvae were 6.05mm in average total length(TL) with $8+18{\sim}19=26{\sim}27$ myomeres. Melanophores were distributed on the top of head, around the intestine tube and on the dorsal and ventral margins of the tail, but none on the pectoral fins. The larva(7.1mm in TL) consumed all the yolk and oil globule, in 7 days after bearing, and jaw bones were more rapidly ossified than vertebrae and cranium. In 21 days after bearing, the larvae averaged 8.50mm in TL and the caudal notochord flex $45^{\circ}$. Fin development in S. inermis larvae proceeds in the following sequence: caudal, pectoral, dorsal, anal and ventral fin. Individuals $17.6{\sim}20.8mm$ in TL($65{\sim}69$ days after bearing) are regarded as the juvenile stage. All fins bones was nearly completed in this stage.

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K-Ar ages and Geochemistry for Granitic and Volcanic Rocks in the Euiseong and Shinryeong Area, Korea (의성-신령지역의 화강암류 및 화산암류에 대한 K-Ar 연대)

  • Kim, Sang Jung;Lee, Hyun Koo;Itaya, Tetsumaru
    • Economic and Environmental Geology
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    • v.30 no.6
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    • pp.603-612
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    • 1997
  • Cretaceous sedimentary-volcanoclastic formations of the Kyeongsang Supergroup were intruded by granitic rocks in the late Cretaceous and early Tertiary. In the Euiseong and Shinryeong area, these intrusives have various compositions including gabbro, diorite,biotite granite and feldspar porphyry. Associated volcanic rocks consist of two chemically distinct types: the bimodal suite of basalt and rhyolite in the Keumseongsan caldera, and the felsic suite of andesite and rhyolite in the Sunamsan-Hwasan calderas. Most rocks are subalkaline, and follow a typical differentiation path of the calc-alkaline magma. The granitic rocks can be distinguished chemically from the volcanics by high Zr/Y ratios. Differences in Zr/Y and K/Y ratios between the two volcanic suites can be accounted for by mantle source and fractionation. Chondrite-normalized trace element abundances of granitic rocks are depleted in Th and K, whereas those of the Keumseongsan rhyolites are depleted in Sr and Ti. Rb, La and Ce is enriched in rhyolites of the Sunamsan-Hwasan calderas. $Rb-SiO_2$ and Rb-Y+Nb discrimination diagrams suggest that the intrusives and volcanics have a volcanic arc setting. K-Ar ages indicate four plutonic episodes : diorite (89 Ma), granite (66~62 Ma), granite and porphyry (55~52 Ma) and gabbro (52~45 Ma), and two volcanisms : bimodal basaltic and rhyolitic volcanism (71~66 Ma) in the Keumseongsan caldera, and felsic andesitic and rhyolitic volcanism (61~54 Ma) in the Sunamsan-Hwasan calderas. Geochemical and age data thus suggest that the igneous rocks are related to several geologic episodes during the late Cretaceous to early Tertiary.

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A Study on Chemical Compositions of Sediment and Surface Water in Nakdong River for Tracing Contaminants from Mining Activities (광해오염원 추적을 위한 낙동강 지역 퇴적물 및 하천수의 화학조성 연구)

  • Kim, Jiyun;Choi, Uikyu;Baek, Seung-Han;Choi, Hye-Bin;Lee, Jeonghoon
    • Journal of the Korean earth science society
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    • v.37 no.4
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    • pp.211-217
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    • 2016
  • There have been found mine tailings, wastes, and mining drainage scattered in the area of Nakdong River due to the improper maintenance of the abandoned mines. These contaminants can flow into rivers during the heavy rain periods in summer. Along the study area beginning Seokpo-myeon, Bonghwa-gun of Gyeongsangbuk-do untill Dosan-myeon, Andong-si, there are one hundred five mines including sixty metalliferous mines and forty-five nonmetal mines, which can adversely affect the adjacent rivers. To verify the contamination, we collected sediments, seepage water and surface water for a year both in rainy season and dry season. This study found that sediments, containing high concentrations of heavy metals caused by mining activities, are dispersed throughout the entire river basin (68 sample points with pollution index, based on the concentration of trace element, (PI) >10 among the total of 101 samples). The results of river water analysis indicated the increased concentrations of arsenic and cadmium at branches from Seungbu, Sambo, Okbang and Janggun mine, which concerns that the river water may be contaminated by mining drainage and tailing sediments. However, it is difficult to sort out the exact sources of contamination in sediments and waters only by using the chemical compositions. Thus the control of mining pollution is challenging. To prevent water from being contaminated by mining activities, we should be able to divide inflow rates from each origin of the mines. Therefore, there should be a continued study about how to trace the source of contaminants from mining activities by analyzing stable isotopes.

Evaluation of Microleakage and Penetration Ability of Flowable Resin in Occlusal Fissure (유동성 레진의 교합면 열구내 침투도와 미세누출 평가)

  • Cheong, Hyeran;Im, Sungok;Lee, Sangho;Lee, Nanyoung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.44 no.1
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    • pp.89-98
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    • 2017
  • The aim of this study was to compared the penetration scores and microleakage levels of flowable resin in comparison to resin-based pit and fissure sealant. A total 120 extracted premolars were used and classified with group I (resin-based pit and fissure sealant), group II (flowable resin), group III (bonding agent + flowable resin), IV (fissurotomy + bonding agent + flowable resin) depending on the materials and the application methods. The penetration scores of the group treated with flowable resin following fissurotomy were similar to those of the group treated with resin-based sealant, but higher compared to those of the other groups treated with flowable resin without fissurotomy or bonding agent. The group treated with flowable resin following fissurotomy also exhibited the lowest microleakage levels. The group treated with resin-based sealant and the one treated with a bonding agent prior to the application of flowable resin showed similar microleakage levels. In conclusion, flowable resin may be applied as a pit and fissure sealant, and its application along with fissurotomy could increase the penetration scores.