13). Analysis of a purified preparation of eCG revealed that its $\beta$ -subunit consists of 149 amino acids, which was confirmed by the molecular cloning of its cDNA. There seem to be at least four to six, or even as many as 11, O-glycosylation sites on the extended C-tenninal region of the eCG $\beta$-subunit. Interestingly, eCG is a unique member of this family, as it appear to be a single molecule that possesses both LH- and FSH-like activities. Using the cDNA prepared from mRNA extracted from equine placental and pituitary tissues, we cloned the cDNA of eCG $\alpha$- and $\beta$ -subunits and eFSH $\beta$ -subunit. The mRNA expression of each subunit seems to be independently regulated, which may account for differences in the quantities of $\alpha$ - and $\beta$ -subunits in the placenta and pituitary. Thus, eCG is a distinct molecule from the view points of its biological function and glycoresidue structures. Recombinant eCGs including the mutants which lack oligosaccharides will be useful tools for analyzing the structure-function relationships of gonadotropins in the horse as well as other species. Similar experiments will also clarify the proposed structure and biological functions for the glycoprotein hormones. These experimental are now possible, and hopefully a resolution of the existing controversy will be forthcoming in the near future.
This paper presents a quantitative evaluation of water permeability in concrete with cold joint considering mineral admixture and loading conditions. Concrete samples with OPC (Ordinary Portland Cement) and GGBFS(Ground Granulated Blast Furnace Slag) are prepared considering 0.6 of W/C ratio and 40% of replacement. 30% and 60% loading levels for compression and 60% loading level for tension are induced to concrete samples. In compression conditions, the permeability in control case shows $2.41{\times}10^{-11}m/s$ in OPC concrete, and it changes to $2.07{\times}10^{-11}m/s$ (30% of peak) and $2.36{\times}10^{-11}m/s$ (60% of peak). The results in GGBFS concrete shows the same trend, which yields $2.17{\times}10^{-11}m/s$ (control), $1.65{\times}10^{-11}m/s$ (30% of peak), and $1.96{\times}10^{-11}m/s$ (60% of peak), respectively. In tensile conditions, the permeability increases from $2.37{\times}10^{-11}m/s$ (control) to $2.67{\times}10^{-11}m/s$ (60% of peak) while that in GGBFS concrete increases from $2.17{\times}10^{-11}m/s$ (control) to $2.24{\times}10^{-11}m/s$ (60% of peak). Permeability coefficients decreases in 30% of compressive level but increases in 60% level, while results in tensile level increases rapidly. This shows pore structure in concrete is condensed and with loading and permeability increases due to micro-cracking. Permeability evaluation considering the effects of loading conditions, cold joint, and GGBFS is verified to be important since water permeability greatly changes due to their effects.
Purpose: The aim was to investigate the effect of implant thread designs on the stress dissipation of the implant. Materials and methods: The threads evaluated in this study included the V-shaped, buttress, reverse buttress, and square-shaped threads, which were of the same size (depth). Building four different implant/bone complexes each consisting of an implant with one of the 4 different threads on its cylindrical body ($4.1mm{\times}10mm$), a force of 100 N was applied onto the top of implant abutment at $30^{\circ}$ with the implant axis. In order to simulate different osseointegration stages at the implant/bone interfaces, a nonlinear contact condition was used to simulate immature osseointegration and a bonding condition for mature osseointegration states. Results: Stress distribution pattern around the implant differed depending on the osseointegration states. Stress levels as well as the differences in the stress between the analysis models (with different threads) were higher in the case of the immature osseointegration state. Both the stress levels and the differences between analysis models became lower at the completely osseointegrated state. Stress dissipation characteristics of the V-shape thread was in the middle of the four threads in both the immature and mature states of osseointegration. These results indicated that implant thread design may have biomechanical impact on the implant bed bone until the osseointegration process has been finished. Conclusion: The stress dissipation characteristics of V-shape thread was in the middle of the four threads in both the immature and mature states of osseointegration.
In this study, we isolated, characterized, and compared the vasa homologous genes of diploid and triploid Paragonimus westermani and localized VASA homologous proteins in both lung fluke types. Open reading frames of Pw-vasa-2n and Pw-vasa-3n were of 1812 bp, and encoded deduced proteins of 622 amino acids with calculated molecular weights of 69.0 kDa and 68.9 kDa and pI's of 9.11 and 9.03, respectively. A comparison of these two VASA deduced protein sequences showed that only 6 of the 622 amino acids differed. The deduced sequences of Pw-VASA-2n and Pw-VASA-3n contained eight consensus sequences characteristic of the DEAD-box protein family and their N-terminal regions contained four arginine-glycine-glycine (RGG) motifs. These two lung fluke VASA-like proteins were more similar to those of other VASA proteins than to those of other DEAD-family proteins isolated from several organisms (planarian, zebra fish, mouse, and human). vasa homologous gene transcription and VASA protein expressions in triploid type lung flukes was slightly stronger than in the diploid type. Immunostaining showed that testes and a portion of the ovaries of both diploid and triploid lung flukes reacted strongly to anti-Pw-VASA antibody.
There is a rapid increase in the use of digital video information in recent years, it becomes more important to manage video databases efficiently. The development of high speed data network and digital techniques has emerged new multimedia applications such as internet broadcasting, Video On Demand(VOD) combined with video data processing and computer. Video database should be construct for searching fast, efficient video be extract the accurate feature information of video with more massive and more complex characteristics. Video database are essential differences between video databases and traditional databases. These differences lead to interesting new issues in searching of video, data modeling. So, cause us to consider new generation method of database, efficient retrieval method of video. In this paper, We propose the construction and generation method of the video database based on contents which is able to accumulate the meaningful structure of video and the prior production information. And by the proposed the construction and generation method of the video database implemented the video database which can produce the new contents for the internet broadcasting centralized on the video database. For this production, We proposed the video indexing method which integrates the annotation-based retrieval and the content-based retrieval in order to extract and retrieval the feature information of the video data using the relationship between the meaningful structure and the prior production information on the process of the video parsing and extracting the representative key frame. We can improve the performance of the video contents retrieval, because the integrated video indexing method is using the content-based metadata type represented in the low level of video and the annotation-based metadata type impressed in the high level which is difficult to extract the feature information of the video at he same time.
The use of laser in the treatment of soft tissue minimizes hemorrhage, provides better view of the operating field, and thereby minimizes operating time. Also, there will be far less post-operative swelling, pain and scar formation, and sterilizing effect are shown in some portions of the wound site. All these advantages of laser therapy contribute to its widespread use in the field of medicine and dentistry. Regarding such facts, we used CO2 laser of different watts in gingivectomy for white rats to compare initial healing process. For the control group, the least amount of output in performing gingivectomy(4watts) was offered, and for the experimental group, 6watts was given. Animals were sacrificed on the second, third days, 1 weeks, 2 weeks, and 3 weeks after operation, and their specimens were histologically analyzed. The following results were obtained: 1. Blood clot of small size was observed in both the control and experimental groups after two days, and no more thereafter. 2. In both the control and experimental groups, the inflammation zone size was the greatest after two days, and it decreased gradually to become almost invisble by the second week. The experimental group showed larger size of inflammation zone during second and third days: however, there was no difference after one week. 3. Granulation tissue in both the control and experimental groups showed gradual maturation with time, and by the second week, it was almost replaced by normal connective tissue. By the third week, complete healing pattern was observed. The experimental group showed larger granulation tissue than the control group until the third day, but there was no significant difference after one week. 4. In both the control and experimental groups, gingival epithelialization began on the second day. After one week, regeneration of rete peg and partial formation of junctional epithelium were observed: by the second week, keratinization of oral sulcular epithelium began, and it was completed by the third week.
Kim, Hyeon-Tae;Lee, Min-HO;qasim, Waqas;Lee, Yong-Jin;Kim, Won-Joong;Yoon, Yong-Cheol
Journal of agriculture & life science
/
v.52
no.6
/
pp.81-88
/
2018
This study analyzed the characteristics of 6 recyclable bulking agents which can replace sawdust and rice hulls that are commonly used in livestock manure composting. The content of all the hazardous chemicals found in the bulking agents used in this experiment was between 0.0 and 34.1 ppm, which was noticeably lesser than the maximum content levels of hazardous substances 5.0~900.0 ppm prescribed by the Rural Development Administration(RDA), which means that the bulking agents would be able to replace sawdusts and rice husks. The water content of the bulking agents ranged from a minimum of 12.4% to a maximum of 16.6% in the original state, which was much less than 60%, the optimal moisture content. These results indicate that they would be able to function fully as bulking agents. Their water absorption rate was in the range of minimum 31.9% ~ maximum 600.0%, which showed huge differences among the types of bulking agents. The most appropriate bulking agent in terms of water absorption rate was wood pellets. It was the highest in the wood briquette manufactured by A, which was followed by C and B in the order. The shear strength of the 5 types of bulking agents in the original state was 271.7 N on an average, the highest figure of all, except for the oak briquette whose shear strength could not be measured and the wood pellets in the absorption state. The shear strength in the dry and absorption states then followed, and were 78.0 N and 27.7 N on an average, respectively. The wood briquette of A recorded considerably lower shear strength than that of B and C. Overall, shear strength tended to increase according to lower water absorption rates. Since pine wood chips and oak cuts have relatively greater shear strength even in the absorbing state than the other materials, they will be able to endure some power in the case of stirring with pig droppings.
Organic farming practices including loach based ecosystem-farming have been demonstrated to be effective in conjunction with rice farming to increase yield and quality. This new form of farming combines agriculture and fishery and is quickly developing into a new industry. The current study investigated the effect of rice-fish mixed farming system on the vegetation-diversity function. Vegetation within the four study sites was surveyed and analyzed based on plant taxonomy. The vegetation survey demonstrated that 127 taxa of 38 families, 100 genera, 107 species, and 20 varieties occurred within the study sites. A total of 15 plant species taxa occurred in the rice-fish mixed paddy fields with a fish habitat and did not occur in the conventional paddy field lacking fish habitat. This difference is thought to arise from differences in moisture requirements for vegetation. Life form analysis demonstrated differences in hemicryptophytes, therophytes, and hydrophytes according to fish habitat. The naturalized plants identified were also determined to be species widely distributed throughout Korea. Frequency analysis demonstrated that the rice-fish mixed paddy fields with a fish habitat had a high ratio of both obligate and facultative wetland plants relative to the conventional paddy field. Based on the study results, it is likely that vegetation-diversity will increase with environment diversity. However, no statistical significance was observed according to paddy types. Future research should aim to identify additional environmental factors, including the existence of fish habitat, habitat area, depth of fish habitat, hydrological parameters, water quality, and paddy soil environment, to enhance vegetation-diversity and biocultural diversity.
Korean Journal of Agricultural and Forest Meteorology
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v.24
no.1
/
pp.48-61
/
2022
South Korea is quite vegetation rich country which has 63% forests and 16% cropland area. Massive NOx emissions from megacities, therefore, are easily combined with BVOCs emitted from the forest and cropland area, then produce high ozone concentration. BVOCs emissions have been estimated using well-known emission models, such as BEIS (Biogenic Emission Inventory System) or MEGAN (Model of Emission of Gases and Aerosol from Nature) which were developed using non-Korean emission factors. In this study, we ran MEGAN v2.1 model to estimate BVO Cs emissions in Korea. The MO DIS Land Cover and LAI (Leaf Area Index) products over Korea were used to run the MEGAN model for June 2012. Isoprene and Monoterpenes emissions from the model were inter-compared against the enclosure chamber measurements from Taehwa research forest in Korea, during June 11 and 12, 2012. For estimating emission from the enclosed chamber measurement data. The initial results show that isoprene emissions from the MEGAN model were up to 6.4 times higher than those from the enclosure chamber measurement. Monoterpenes from enclosure chamber measurement were up to 5.6 times higher than MEGAN emission. The differences between two datasets, however, were much smaller during the time of high emissions. More inter-comparison results and the possibilities of improving the MEGAN modeling performance using local measurement data over Korea will be presented and discussed.
Volatility plays a central role in both academic and practical applications, especially in pricing financial derivative products and trading volatility strategies. This study presents a novel mechanism based on generalized autoregressive conditional heteroskedasticity (GARCH) models that is able to enhance the performance of intelligent volatility trading systems by predicting Korean stock market volatility more accurately. In particular, we embedded the concept of the volatility asymmetry documented widely in the literature into our model. The newly developed Korean stock market volatility index of KOSPI 200, VKOSPI, is used as a volatility proxy. It is the price of a linear portfolio of the KOSPI 200 index options and measures the effect of the expectations of dealers and option traders on stock market volatility for 30 calendar days. The KOSPI 200 index options market started in 1997 and has become the most actively traded market in the world. Its trading volume is more than 10 million contracts a day and records the highest of all the stock index option markets. Therefore, analyzing the VKOSPI has great importance in understanding volatility inherent in option prices and can afford some trading ideas for futures and option dealers. Use of the VKOSPI as volatility proxy avoids statistical estimation problems associated with other measures of volatility since the VKOSPI is model-free expected volatility of market participants calculated directly from the transacted option prices. This study estimates the symmetric and asymmetric GARCH models for the KOSPI 200 index from January 2003 to December 2006 by the maximum likelihood procedure. Asymmetric GARCH models include GJR-GARCH model of Glosten, Jagannathan and Runke, exponential GARCH model of Nelson and power autoregressive conditional heteroskedasticity (ARCH) of Ding, Granger and Engle. Symmetric GARCH model indicates basic GARCH (1, 1). Tomorrow's forecasted value and change direction of stock market volatility are obtained by recursive GARCH specifications from January 2007 to December 2009 and are compared with the VKOSPI. Empirical results indicate that negative unanticipated returns increase volatility more than positive return shocks of equal magnitude decrease volatility, indicating the existence of volatility asymmetry in the Korean stock market. The point value and change direction of tomorrow VKOSPI are estimated and forecasted by GARCH models. Volatility trading system is developed using the forecasted change direction of the VKOSPI, that is, if tomorrow VKOSPI is expected to rise, a long straddle or strangle position is established. A short straddle or strangle position is taken if VKOSPI is expected to fall tomorrow. Total profit is calculated as the cumulative sum of the VKOSPI percentage change. If forecasted direction is correct, the absolute value of the VKOSPI percentage changes is added to trading profit. It is subtracted from the trading profit if forecasted direction is not correct. For the in-sample period, the power ARCH model best fits in a statistical metric, Mean Squared Prediction Error (MSPE), and the exponential GARCH model shows the highest Mean Correct Prediction (MCP). The power ARCH model best fits also for the out-of-sample period and provides the highest probability for the VKOSPI change direction tomorrow. Generally, the power ARCH model shows the best fit for the VKOSPI. All the GARCH models provide trading profits for volatility trading system and the exponential GARCH model shows the best performance, annual profit of 197.56%, during the in-sample period. The GARCH models present trading profits during the out-of-sample period except for the exponential GARCH model. During the out-of-sample period, the power ARCH model shows the largest annual trading profit of 38%. The volatility clustering and asymmetry found in this research are the reflection of volatility non-linearity. This further suggests that combining the asymmetric GARCH models and artificial neural networks can significantly enhance the performance of the suggested volatility trading system, since artificial neural networks have been shown to effectively model nonlinear relationships.
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