• Korean Journal of Animal Reproduction
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• v.25 no.1
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• pp.71-77
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• 2001

This experiment was designed to improve the production efficiency of germ-line chimeric mice from phospholipase C (PLC)-$\beta$3 or peroxiredoxin (Prx) E -targeted ($\Delta$) ES cells by the investigating the manipulation conditions and characteristics of Jl ES cells. Four targeting clones were isolated to investigate the karyotypical and morphological stability prior to injection. All clones ($\Delta$PLC$\beta$-3 C3, $\Delta$Prx II C3, C10 and I5) showed more than 80% euploidism, however, most of $\Delta$PLC$\beta$-3 C3 clones were extensively differentiated compared to the other clones. Nine of 13 $\Delta$Prx II chimeras appeared to have at least 80% chimerism, whereas $\Delta$PLC$\beta$-3 C3 chimeras had 20% chimerism at most. Therefore, the morphological stability of ES cells under stable euploidism might mainly affect the production rate of high-coat chimeric mice. To increase the collection rate of injectable blastocysts (IBs), 5 to 10 week -aged C57BL/6J female mice were sacrificed at 3.5 days post-coitum. Ten week-aged mice were the most optimal IB donors by showing the highest collection rate (2.94/mouse) of injectable blastocysts without increase of non-injectable embryos (0.29/mouse). Foster mothers might be another factor because ICR x C57BL/6J F1 foster mother showed more increased productivity in litter size (2.8 vs. 5.6) and chimera (0 vs. 35.3%) than those of ICR foster mothers. In conclusion, the efficient production of germ-line chimeras mainly depends on the maintenance of ES cell morphology during targeting procedure, and the establishment of manipulation conditions might be a key point to maximize it.

• Journal of Korean Academy of Nursing
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• v.16 no.1
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• pp.89-104
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• 1986

복잡한 사회문제해결에 유용한 접근방법의 하나인 계층분석과정은 수학자인 사티(Saaty)가 개발한 것으로서 이미 사회과학의 여러 분야에 적용되고 있다. 본 논문의 목적은 이 계층분석과정을 간호교육과 관련된 문제에 적용해 봄으로써 이 방법이 실제 문제해결을 위한 유용한 접근방법이 될 수 있는가를 고찰해 보는데 있다. .현대사회문제들은 다양한 관련요소, 그 요소들간이나 다른 문제들과의 복잡한 상호관계, 다수의 의사결정자의 참여, 무한한 대안, 문제의 결과에 대한 불확실성 등으로 인하여 단순한 모델이나 간단한 논리적 혹은 직관적인 판단에 준하여 일시에 해결하기 어려운 경우가 많다. 이러한 상황 하에서 문제와 관련된 요소들을 찾아내고 그 요소들을 계층적으로 분석하여 단계적으로 문제해결에 접근하는 계층분석과정을 활용함으로써 사회과학분야의 문제해결에 유용한 수단이 될 수 있다. 또한 문제와 관련된 요소를 규명하고 계층적으로 분화하는 과정에서 그 문제에 대한 보다 명확한 이해, 분석 및 현상의 흐름을 더 잘 인해하게 된다. 계층분석과정은 1) 계층구조의 설정 2) 구성요소들의 중요도 측정 3) 중요도에 따른 우선 순위의 산정 4) 우선 순위의 일관성 검토과정으로 요약된다. 본 논문에서는 이러한 과정을 통하여 미국 간호계에서 심각한 문제로 대두되어온 기본교육제도상의 쟁점(Issue of Entry into Practice）에 대한 문제 해결책이 마련되는지를 시도하였다. 즉, 기본교육 제도에 영향을 미칠 수 있는 요인들을 규명하여 계층화하고 계층내에 위치하는 구성요소들의 수평적 관계와 계층간의 수직적 관계를 체계적으로 검토하여 간호교육의 미래를 예측해 보았다. 간호교육제도에 영향을 미칠 수 있는 주요 요인들의 계층화는 거시적 환경차원(factors), 관련 이해자 집단차원(actors), 이해자 집단의 목표차원(objectives), 목표달성을 위한 정책차원(Policies), 그리고 간호교육제도의 미래를 예견한 시나리오(scenario)로 이루어졌다. 각 계층을 따라 단계적으로 중요도를 비교하여 최종적으로 각 시나리오의 우선순위 중요도(priority weight)를 구하였다. 각 시나리오의 우선순위 중요도를 간호교육과 관련된 4가지 차원들(학생, 교수, 교육기관, 직업)을 구성하고 있는 특성들에 가중하여 결과를 해석 하였다. 간호교육의 미래에 정치적(55%)과 경제적(25%) 요인이 가장 강한 영향을 미치고, 관련자중에서는 병원(38%) 의사(33%) 그리고 정부(23%)의 순으로 영향을 미친다고 분석되었다. 의외로 간호원 자신들은 큰 영향력이 없는 것으로 보여졌다. 4개의 시나리오 중에서는 그 우선순위 중요도가 현상유지(33%) 자발적4년제(32%), 강제 4년제(19%), 그리고 2~3년제의 우위(18%) 순으로 나타났다. 최종 시나리오 결과를 요약하면 1) 전체 학생수는 감소하나 양질의 간호학생수는 증가하며 2) 박사학위를 소지한 간호학 교수 및 전체 교수의 수가 증가할 것이다. 3) 전체 간호교육기여의 수는 약간 줄 것이며 그중 4년제 기관이 증가하고 반면에 2~3년제의 기관은 감소할 것이다. 4) 전문간호원이 되기 위한 입학용이도에는 별차이가 없겠으나 간호원들의 보수, 지위 및 자율성이 증가하면서 전반적인 간호의 질이 향상될 것이다. 계층분석과정의 적용으로 미국 간호교육계의 가까운 미래를 위와 같이 예견하여 보았으나 이 과정은 예견 뿐 만 아니라 일반적인 의사결정이나 문제 해결의 도구이외에도 복잡한 사회문제의 본질을 분석, 이해함으로써 보다 정화한 정책문제를 규정하는 데에도 유용할 것이다.

• Journal of Food Hygiene and Safety
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• v.21 no.3
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• pp.171-180
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• 2006

Peroxisome proliferatorr-activated receptor-gamma $(PPAR{\gamma})$ must form a heterodimer with the retinoid-X receptor (RXR) to bind DNA, and its transcriptional activity is thought to be maximized by ligands specific for either receptor. Activated $(PPAR{\gamma})$ and $(PPAR{\gamma})$ ligands may influence tumor growth through regulation of the tumor suppressor PTEN. Our aim in this study was to determine whether co-stimulation with the $(PPAR{\gamma})$ ligand, ciglitazone, and RXR ligand can synergistically upregulate PTEN in human acute promyelocytic leukemia (APL) cells and consequently potentate the inhibition of cell growth and cell cycle progression of these cells. Human leukemia cell line, HL-60 cells were exposed to all-trans-retinol and ciglutazone. The PTEN expression was measured as the level of PTEN mRNA expression by RT-PCR and as the level of PTEN expression by western blot analysis. Cell cycle analysis was carried out by a propidium iodide (PI) staining method and analyzed with a FACScan. The $(PPAR{\gamma})$ ligand, ciglitazone, and the RXR ligand, retinoic acid, upregulated PTEN expression by HL-60 cells in time- and dose-dependent manners, respectively. This was significantly enhanced by a combination of both ciglitazone and retinoic acid. Moreover, these compounds synergistically induced arrests of both cell growth and the $G_l$ phase of the cell cycle. Thus, the activation of the $(PPAR{\gamma})$:RXR heterodimer may represent a regulatory pathway for human leukemia cells and there may be important roles for $(PPAR{\gamma})$ and RXR ligands in prophylactic and therapeutic approaches fur controlling leukemia through the upregulation of PTEN.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.7
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• pp.426-439
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• 2017

This review summarizes advantage and limitation in infrared spectroscopy and computational chemistry to understand rhizospheric interaction among organic acids, oxyanions and metal oxides. Since organic acids and metal oxides determine dynamics of oxyanions in the soil environment, knowledge of fundamental mechanisms is a prerequisite for understanding the interactions at soil-water interface. Attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) is a powerful tool to measure the interfacial reactions. However, the ATR-FTIR measurements are abstruse, because the optical characteristics for measurements are variable depending on the experimental setup. In addition, spectral overlapping is a primary obstacle to the analysis of the interfacial reaction; thus, it is essential to detect and to deconvolute bands for signal interpretation. In this review, we expained the fundamental principle for spectrum processing, and four band identification methods, such as derivative spectroscopy, two-dimension correlation spectroscopy, multivariate curve resolution, and computational chemistry with example of aqueous phosphate speciation. As a result, spectrum processing and computational chemistry improved interpretation and spectral deconvolution of overlapped spectra in relatively simple systems, but it was still unsatisfactory for the problems in more complexed system like nature. Nevertheless, we believed that our challenge would contribute practically to develop adequate analytical procedure, signal processing and protocols that could help to improve interpretation and to understand the interfacial interactions of oxyanions in natural systems.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.4
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• pp.446-453
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• 2018

The study examines corporate research & development (R&D) expenditure in modern finance. Firms may face one of the essential issues to maintain their optimal levels of R&D expenditures in order to increase corporate profit. Accordingly, financial determinants that may influence R&D spending are statistically tested for firms listed on the KOSPI stock market during the period from 2010 to 2015. Financial determinants which may discriminate between firms in high-growth and low-growth industries are examined on a relative basis. Explanatory variables including one-period lagged R&D expenses (Lag_RD), cross-product term between the Lag_RD and type of industry (as a dummy variable), and advertising expenses (ADVERTISE) significantly influenced corporate R&D intensity. Moreover, high-growth firms in domestic capital markets showed higher Lag_RD, profitability (PROF) and foreign equity ownership (FOS) than their counterparts in low-growth sectors, whereas low-growth firms had higher market-value based leverage (MLEVER) and ADVERTISE. Overall, these results are expected to influence decision-making of firms concerning the optimal level of R&D expenditure, which may in turn enhance shareholder wealth.

• Proceedings of the KSRS Conference
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• 2007.03a
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• pp.43-48
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• 2007

본 연구는 벼 군락의 분광반사율 지표를 측정할 수 있는 인공광원을 사용하는 능동형 광학 센서인 GNDVI 를 활용하여 생육시기별 식생지수와 엽 질소함량과의 관계를 구명하여 벼 군락의 엽 질소함량을 추정하고자 하였다. 농업과학기술원 답작 포장에서 공시품 종인 추청벼를 이용하여 난괴법 3반복으로 시험구 배치를 하고 질소 4수준 3반복으로 실험을 수행하였다. 벼 생육시기별 GNDVI 와 엽 질소함량과의 관계를 2년 간 (2005, 2006)의 자료를 통하여 분석해 보았다. 2005년의 경우 벼 생육시기동안 9시기의 GNDVI값과 그 당시 시료를 샘플링하여 분석한 엽 질소함량과의 관계를 벼 출수전과 출수후로 구분하여 분석해 본 결과 GNDVI 값은 출수전 ($r=0.78^{***}$ n=60) 보다 출수후 ($r=0.89^{***}$, n=59) 가 엽 질소함량과의 상관계수가 높았다. 2006년은 20시기동 안 생육시기별 식생지수와 엽 질소함량과의 상관 분석한 결과 착근기 (6월 5일) $r=0.84^{***}$, 유수분화기 (7월 11일) $r=0.95^{***}$, 출수기 (8 월 16일) $r=0.87^{***}$, 수확기 (10월 13일) $r=0.90^{***}$ 으로 출수전의 경우 7월 11일이 상관계수가 가장 높았고， 이 결과는 2005년 동일시기 (7월 11일) 식생지수와 벼 엽 질소함량과의 상관계수가 가장 높았던 ($r=0.91^{***}$) 것과 일치하였다. 벼 생육시기 변화에 따른 식생지수와 엽 질소 흡수량과의 관계를 살펴보았는데 벼 출수전의 경우 GNDVI는 7월 11일 에 엽 질소 흡수량과의 상관계수 ($r=93^{***}$)가 가장 높은 결과를 보였고 출수후의 경우에는 시기에 따라 상관계수가 고르게 높게 나타났다. 엽 질소함량과의 상관관계가 높았던 2005년，2006년 7월 11일 식생지수 데이터를 함께 이용하여 엽 질소함량과의 관계를 추정식으로 작성하였다. GNDVI를 이용하여 2005년과 2006년 실측한 엽 질소함량 값과 추정 값을 비교해 본 결과 2005년과 2006년의 결정계수가 각각 0.88, 0.94로 2006년이 더 예측률이 높게 나타났다. GNDVI 값을 이용하여 엽 질소함량 추정값과 실측값을 비교해 본 결과 결정계수가 0.86으로 추정값과 실측값이 근접하게 분포하였다.

• The Journal of the Petrological Society of Korea
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• v.7 no.3
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• pp.161-176
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• 1998

There are exposed Samrangjin Tuff and intracaldera intrusions, of which rhyolitic rocks emplaced as postcollapsed central and ring intrusions within the Samrangjin caldera, and fine-grained granodiorite and biotite granite as regional tectonic intrusions nearby. The Samrangjin Tuff and the rhyolitic rocks are of a single Samrangjin magmatic system. Flow-banded rhyolite among rhyolitic rocks was emplaced in the outer part of the ring intrusions, rhyodacite in the inner part of the eastern ring, and porphyritic dacite and dacite porphyry in the inner part of the northwestern ring. Totally the Samrangjin Tuff and the rhyolitic rocks range from rhyolite to dacite in chemical composition. The Rb-Sr isotopic data of the Samrangjin Tuff and the rhyolitic rocks yield an age of $80.8{\pm}1.5(2{\sigma})$ Ma with the initial $^{87}Sr/^{86}Sr$ ratio of $0.70521{\pm}0.00010(2{\sigma})$. The continuous compositional zonations generally define a large stratified magma system in the postcollapse magma chamber. The Sr isotopic data suggest that the compositional zonations might have resulted from the fractional crystallization of a parental dacitic magma.

• The Journal of the Petrological Society of Korea
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• v.4 no.2
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• pp.153-167
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• 1995

Emplacement ages for the granite plutons of the Jurassic and the Cretaceous times in the central Ogcheom Fold Belt were determined by Rb-Sr whole rock and mineral isocheon methods. In addition mineral ages for the plutons were determined by K-Ar and fission track methods. In turn, thermal histories and uplifting rates of the granitic bodies are elucidated from the isotopic ages. The Jecheon(~203 Ma) and Mungyeong(at lest~200 Ma) granites of the Jurassic and the Muamsa, Wolagsan and Daeyasan granites(~110 Ma) of the Cretaceous show high strontium initial ratios [$(^{87}Sr/^{86}Sr)_1$0.7100],suggesting that the granitic magmas have been generated by partial melting of crustal materials (S-type), or by mixing of mantle and crustal materials. Only mineral ages of the Sogrisan and Hyeongjebong granites (~90 Ma) were determined by K-Ar method, and petrogenesis of them were not defined yet. The two Jurassic granite plutons were cooled rapidly down to $300^{\circ}C$, right after the plutons were slowly cooled down since then, due to their deep emplacment. During the Middle Cretaceous period, the Jurassic Mungyeong granitic pluton was intruded and thermally affected much by the surrounding Wolagsan and Daeyasan granites. Accordingly the Rb-Sr mineral age, K-Ar hornblende and biotite ages of the Mungyeong granite appear to be reduced or reset due to the thermal effects above their blocking temperatures. All the cretaceous granites have been cooled much ore simply and rapidly down than the Jurassic ones below $300^{\circ}C$, owing to their shallow emplacement.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.22 no.3
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• pp.139-146
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• 2012

Natural hydroxyapatite powder was obtained from the calcination of bovine bones and its porous compacts were fabricated by pressureless sintering at 1100 and $1200^{\circ}C$ for 1h. To evaluate and compare their biocompatibility with porosity, we investigated the support of osteoblast cells growth and cytotoxicity using the MG-63 cell line model in vitro. Sintered hydroxyapatite ceramics have a porous microstructure with a relative density of 65 % at $1100^{\circ}C$ and 82 % at $1200^{\circ}C$. Cells adherence to the surface of hydroxyapatite ceramics was observed in a day after the cell culture, and the spreading of cytoplasm around the nucleus was shown after 3 day cell culture. Most of cells were extended to the surface of hydroxyapatite through the wide area. Cell viability was nearly the same till 3 days culturing. But the rate of cell growth is higher in the specimen sintered at $1100^{\circ}C$ than that of $1200^{\circ}C$. It indicates that the porosity is an important factor to enhance the cell viability in the porous hydroxyapatite ceramics derived from bovine bones.

• The Journal of the Petrological Society of Korea
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• v.19 no.1
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• pp.67-87
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• 2010

This article deal with petrotectonic setting and petrogenesis from petrography and chemical analyses of the Cretaceous volcanic and intrusive rocks in the Cheolwon basin. The volcanic rocks are composed of basalts in Gungpyeong Formation, Geumhaksan Andesite, and rhyolitic rocks (Dongmakgol Tuff, Rhyolite and Jijangbong Tuff), and intrusive rocks, Bojangsan Andesite, granite porphyry and dikes. According to petrochemistry, these rocks represent medium-K to high-K basalt, andesite and rhyolite series that belong to calc-alkaline series, and generally show linear compositional variations of major and trace elements with increase in $SiO_2$ contents, on many Harker diagrams. The incompatible and rare earth elements are characterized by high enrichments than MORB, and gradually high LREE/HREE fractionation and sharp Eu negative anomaly with late strata, on spider diagram and REE pattern. Some trace elements exhibit a continental arc of various volcanic arcs or orogenic suites among destructive plate margins on tectonic discriminant diagrams. These petrochemical data suggest that the basalts may have originated from basaltic calc-alkaline magma of continental arc that produced from a partial melt of upper mantle by supplying some aqueous fluids from a oceanic crust slab under the subduction environment. The andesites and rhyolites may have been evolved from the basaltic magma with fractional crystallization with contamination of some crustal materials. Each volcanic rock may have been respectively erupted from the chamber that differentiated magmas rose sequentially into shallower levels equivalenced at their densities.