• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.38-38
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• 2018

최근 호두 기내 소비량은 급증하나 대부분 실생묘로 조림되어 결실까지 장기간 소요되며, 무성 번식률이 낮아 보급확대가 어렵다. 본 연구는 호두나무 관행 묘목 번식법 개선이 가능한 우량 국산묘 대량증식 기술 개발로 최적 배양조건을 구명하고자 수행하였디. 호두나무 등 목본류는 식물 조직배양 기본배지에서 산화방지제를 첨가하여 사용하는 경우, 배양체에서 생기는 페놀 화합물(Phenolic compound)의 작용을 억제하여 배양 식물체의 갈변(Browning) 또는 흑변(Blackening) 현상을 감소시키는 효과가 있다. 페놀 추출물은 배지로 빠져나와 갈변시키며 생존율을 저하시키는 원인이 되고 이를 제거하는 산화방지제는 배양 식물체의 생산효율을 높이는 효과를 나타내는 강력한 항산화제(Antioxidant) 역할을 한다. 호두나무의 액아를 배양하게 되면 조직 및 배지의 갈변화가 심하게 일어나는데, 휴면 정도가 깊을 수록 정단에서 추출되는 페놀성 물질의 함량이 증가하며, 식물 조직 내 페놀성 물질의 함량 정도와 배양의 고사율과는 상관관계가 있다. 이러한 식물 배양체에서 생기는 페놀 화합물에 의해 발생되는 배양상의 문제점을 해결하기 위하여 계대배양을 자주해 주거나 활성탄(Activated charcoal), Ascorbic Acid 등의 첨가물을 배지에 추가하는 방법이 있다. 이에 따라 호두 '신령' 품종의 액아배양의 페놀집적을 제거하기 위해 배지 내에 산화방지제 첨가물질 등을 농도별로 처리하였다. 호두나무 액아 배양 후 8주차 산화화방지제 첨가물질의 농도별 처리 효과는 생존율은 85.3%를 보였으며, 신초 형성율 76.4%, 신초수 1.9개/주, 신초장 27mm/주의 양호한 결과를 나타냈으며, 페놀 제거 및 유식물체 고사 억제 효과를 보였다.

• Journal of Bio-Environment Control
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• v.11 no.2
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• pp.61-66
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• 2002

Double cropping system in a year in Kyoho grapes (Vitis labruscana L.) has currently been attempted in the plastic greenhouse. One of the problems in double cropping system is the promotion of bud break in summer season and shoot fertility. Effects of the control of soil moisture tension near the root zone and treatments of bud dormancy breaking agents on bud breaking in summer were examined to promote the bud break for the second fruiting. The lignification of shoots was induced in July or August by the control of soil moisture tension in root zone environment. The first shoot growth was almost the same as that in common plastic greenhouses. The highest bud break value appeared in the plot of cyanamide chemicals mixed with merit blue as over 75% bud break rate. The bud break rate in the discontinuing plot in irrigation showed significantly higher in bud break than that in the continuing plot in irrigation. Despite of the final high bud break rate, the time of bud break was irregular.

• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.290-294
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• 2007

Interspecific hybrid seedlings by artificial crossing of Genus Quercus (Q. serrata, Q. dentata, Q. mongolica var. crispula, Q. aliena) were planted at nursery in Tottori University. Seedlings of hybrids by natural crossing(Q. fabri and $Q.{\times}mccormickii$) were selected and planted at Hiruzen district in Tottori University. Artificial interspecific hybrid $F_1$ and natural hybrid $F_1$ bloomed when they were 4 years old and 3 years old, respectively. The pollen fertility and seed viability were investigated from the bloomed individuals in 2001. The germination percentages of the pollens of artificial interspecific hybrid were more than 84% except one individual, and the extension of pollen tubes was normal. The normal seed percentages of artificial interspecific hybrid were more than 90% similar to parents. Germination percentages of normal seed of natural crossing family were more than 64%, respectively, and selfed offsprings of Q. fabri, and $Q.{\times}mccormickii$ hade high reproductive ability.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.380-387
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• 2013

The objective of this study is to investigate the effect of elevated $CO_2$ and temperature on the phenology and reproductive ecological characteristics of Bupleurum latissimum. We measured characteristics of 4-year-old B. latissimum grown under control (ambient $CO_2$+ambient temperature, 340~370 ppm) and treatment (elevated $CO_2$+elevated temperature, 690~770 ppm) conditions in a glass house. The period of flowering, fruit appearance and fruit maturing was earlier by 4, 1, 2 days, respectively, under elevated $CO_2$ and temperature. The comparative weight of a single ripe fruit, all ripe fruits and total ripe and unripe fruits was higher per control than treated plants. However the other characteristics, such as no. of peduncle, matured fruits, total fruits per plant and fruit setting rate per plant of B. latissimum were not significantly affected by elevated $CO_2$ and temperature. The germination rate of B. latissimum was higher in control than in treatment plants. These results showed that the phenology of B. latissimuin might be well suited to an earlier growing season, and the response of reproductive characteristics of B. latissimuin is negatively influenced by global warming.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.291-297
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• 1984

The peanut, unlike other plants, has three distinct steps in the reproductive process. These are the flowers, the peg and the fruit. This research was designed in the field to determine the difference of fruiting habit for each of plant types of peanut (Arachis hypogaea L.) in peanut culture limiting region. Eight cultivars representing four plant types, i.e., Virginia, Spanish, Valencia and Shinpung types. Number of pegs per plant produced in order of Spanish, Valencia, Virginia, Shinpung, Virginia type. The Virginia small seed type produced the most pods per plant. The shinpung type showed more percentage of matured pod than other types and early pot-set habit. Distribution of mature pods for each of branch positions to total mature pods occupied 63% on two cotyledonary branchs, 34% on 3rd to 5th branchs, 3% on over 6th branchs. Shinpung type was considered more favorable plant type than other types for breeding of early maturing high yielding variety because of early pot-set and high percentage of mature pod.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.205-210
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• 1994

This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.3
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• pp.285-293
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• 2017

Globally, 1.3 million people develop colon cancer every year, and 600,000 people die each year it. In Korea, colorectal carcinoma was associated with the highest death rate, accounting for 8,380 people, among solid cancers in 2015. Among the various methods for the diagnosis and study of colorectal carcinoma, the results obtained by cytogenetic and molecular genetic methods were compared. Detection rate was 47% in 18q, 40% in 17p, 27% in 22q, and 17% in 10q via CGH; detection rate was 57% in D18S59, 50% in D18S68, 50% in TP53CA, 47% in D18S6940% in D22S274, 37% in D22S283, 27% in D10S187, and 23% in D10S541 with LOH. Microsatellite marker matching rates were 100% in D22S274, 100% in D22S283, 100% in D10S186, 100% in D10S187, 100% in D10S541, 93% in D18S69, 93% in D18S68, 92% in TP53CA, and 89% in D18S59. The agreement rate between the two methods was 94.4% based on positive results using CGH. Based on the advantages of CGH, which was the ability to obtain information regarding the entire tumor genome at once, this experiment could identify the region with significant deletion using CGH and the more limited region LOH, with a completely different approach. LOH in the recurrent high-risk group, 18q21, was helpful in the selection of treatment modalities and in prognostic estimation as well as making the most appropriate decision for treatment. Therefore, it is suggested that LOH with surgical site tissues could be one of the treatment methods for recurrent high-risk group among patients with colorectal carcinoma.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.136-142
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• 2013

Optimal condition for pollen germination was suggested as a basic research of flowering physiology in order to identify the characteristics of flowering and seed setting of rare and endangered Forsythia saxatilis Nakai. Pollen samples were collected during flowering time from the end of March to the beginning of April. First, a suitable germination temperature, medium sucrose concentration and germination time were determined for pollen germination and pollen tube elongation in vitro, and then an optimal pH of culture medium. Pollen germination and tube elongation were significantly different among the levels of germination temperature, sucrose concentration and germination time. Interactive effects were observed between germination temperature and time, germination temperature and sucrose concentration, germination time and sucrose concentration. Pollen germination was the highest at $10^{\circ}C$ and increased with the increase of sucrose concentration, whereas it had no relation with germination time. In addition, pollen germination and tube elongation did not increase at more than 15% of sucrose concentration and 24 hours later. Pollen germination was the highest at pH 5 (20.8%) and the lowest at pH 6 (3.8%). In conclusion, $10^{\circ}C$, 15% sucrose and pH 5 were proposed as the optimal condition for pollen germination 24 hours later of pollen culture.

• Proceedings of the Ginseng society Conference
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• 2005.11a
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• pp.3-18
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• 2005

This paper reports the characteristics of 8 new cultivars for selected from Korean ginseng. The occurance of multi stems were the highest in Yunpoong(45%) and the lowest in Gumpoong(7%), but growth of aerial parts were the highest in Gumpoong and the lowest In Yunpoong among new cultiyars. The ratio of seeds harvest were the highest in Gumpoong(85.4%) and the lowest in Chunpoong(69.1%), but number of seeds per plant were the highest in Yunpoong(108.3ea) and the lowest in Chunpoong(77.5ea) among new cultivars. The ratio of leaf burning were the highest in Chunpoong but the lowest in Yunpoong among new cultivars. In weight distribution of the different parts of the ginseng roots, the ratio of main root were high in Jakyungjong(63.1%) but low in new cultivars(49%-55.9%), but the ratio of lateral root were high in new cultivars(19.3-23.3%), but low in Jakyungjong(13.2%), the ratio of fine root were not different. Root yield declined in the order of Yunpoong, Gumpoong, Gopoong, Chunpoong, Sunpoong, Jakyungjong. The length of main root were the longest in Chunpoong(8,0cm) but the shortest in Yunpoong(6,4cm), The ratio of rusty-root was low in new cultivars(0,2-9,5%), but high in Jakyungiong(16,3%). The grade of red ginseng roots decreased in the order of Chunpoong, Gumpoong, Gopoong, Sunpoong, Yunpoong, Cheongsun, Jakyungjong. The total ginsenoside contents per dry weight in main roots was high in Gumpoong(8.53mg), Yunpoong(8.13mg), Gopoong(7,47mg), but low in Chunpoong(5.73mg), Sunpoong(4.87mg).

• Korean journal of applied entomology
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• v.60 no.1
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• pp.99-103
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• 2021

This study was conducted to investigate the hatching rate of Pochazia shantungensis according to the treatment method of machine oil and the effect on apple quality by spawning. In the effect of machine oil treatment, when 20 times of the machine oil was spread by brush, the average hatching rate was 0.57%, and the sprayed branches showed an average hatching rate of 1%. When the machine oil agent was applied 50 times, the hatching rate was about 35% to spread by brush, whereas the spray treatment showed about 77%, so there were no difference from the non-treatment considering the deviation. There was no statistically significant difference in the quality of fruit even when apple fruits were fruited in the following year in the results of spawning and non-spawning of P. shantungensis in Hongro and Fuji varieties. In addition, there was no statistical significance in the difference between branch breakage and growth caused by spawning of P. shantungensis.