• Journal of Veterinary Clinics
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• v.30 no.2
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• pp.131-133
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• 2013

A 9-year-old, intact female Jindo dog weighing 23 kg was presented to the Veterinary Medical Teaching Hospital of Chungnam National University with a history of two months of ocular discharge. Initial ocular examination showed a 2 cm elongated mass arising from the ventral bulbar conjunctiva of the left eye. Ocular ultrasound, performed to rule out any orbital and intraocular involvement, was negative. Thoracic radiographs and abdominal ultrasonography revealed no abnormalities. Fine needle aspiration (FNA) of mass was performed. Aspiration cytology showed the presence of a round cell population mostly characterized. A moderate amount of eosinophils was present among round cells. The mass was excised and identified hitologically as a mast cell tumor. Six months after surgical treatment, thoracic radiographs and abdominal ultrasonography did not reveal any clinical signs of local recurrence or metastatic disease.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.3
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• pp.95-101
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• 2008

Purpose: Hydrogen peroxide which is one of the reactive oxygen species has been seen to cause various diseases, various cellular disinfections, gene transformation and cell death. The goals of this study were to determine the protective effect of EGCG against $H_2O_2$-induced apoptotic death in conjunctival cell lines. Methods: We measured cell viability by MTT assay and analyzed DNA fragmentation to check up a distinctive feature in cell death and measured the removal ability of free radicals by DPPH free radical scavenging assay and evaluated the oxygen free radical's quantity in the cell by DCFH-DA assay. The mRNA expression in the cell were examined by RT-PCR. Results: Cell viability and free radical scavening activites was significantly increased in dose dependently after cell was exposed to EGCG. And DNA fragmentation and intracellular ROS was decreased. It was showed the mRNA expression which increase of bcl-2, bcl-xL expression and decrease of bax expression. Conclusions: From these results, it suggests that EGCG has an antioxidant effect and protects conjunctival cell lines from the $H_2O_2$-mediated apoptosis through the modulation of the mRNA expression.

• 건강소식
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• v.36 no.3
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• pp.20-22
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• 2012

겨울잠을 자던 개구리가 깨어나는 경칩이 지나고 밤과 낮의 길이가 같아지는 춘분이 오면 기다리고 기다리던 봄이다. 하지만 오는 봄이 고통스러운 사람이 있다. 꽃가루, 황사 등으로 알레르기성 비염을 앓고 있는 사람과 알레르기성 결막염을 앓고 있는 사람이 그 주인공이다.

• The Journal of Korean Society for Radiation Therapy
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• v.14 no.1
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• pp.95-99
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• 2002

I. 목적 : 결막 림프종 환자의 전자선 치료를 위해 Lens shielding block의 제작시 외부 의뢰 제작과 SMC 자체 제작의 장${\cdot}$단점을 비교해보고 제작 과정에 있어 업무의 효율성을 평가하고자 한다. II. 대상 및 방법: Lens shielding block의 제작시 외부의뢰제작과정과 자체 제작 과정을 비교하여 업무의 효율성, 인력의 변화 및 두방법의 장?단점을 분석하였다. 모의치료후 두 방법의 제작 시간을 비교하였으며, 재료의 차이점과 이용방법을 비교하여 개선된 사항을 알아보았다. III. 결과 : Lens shielding block의 자체 제작시 치료 시작시기는 모의치료 실시 후 7일에서 3일로 단축되어 업무의 효율성과 그에 따른 환자의 불편함이 줄어들게 되었다. 또한, 외부의뢰 제작 과정에서 실시되었던 치과와 치과 기공실에 의뢰하던 과정이 없어지고 과내 모의치료실과 공작실에서 작업하게 되어 모든 작업을 과내에서 일률적으로 실시할 수 있게 되었다. 또한, 모의치료를 실시하면서 실시간으로 shielding block을 수정 할 수 있어 정확성이 재고되었고, 반복되는 제작 작업으로 인해 제작 공정이 보편화되고, 손쉬어졌으며, 재료 활용의 효율성이 증대되었다. IV. 결론 : Lens shieiding block의 자체 제작으로 시간이 단축되어 업무의 효율성이 증대되었고 그에 따른 인력소모와 환자의 불편함이 줄어들게 되었으며, 제작자가 모의치료를 통해 바로 block의 오차를 수정할 수 있어 치료의 안정성 및 정확성이 높아졌으므로 결론적으로 의료의 질 개선과 서비스 향상이 이루어졌다.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.2
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• pp.247-253
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• 2015

Purpose: The purpose of this study was to compare and analyze the ocular surface and the palpebral conjunctiva of categorized subjects, which were divided into normal eye group and dry eye group, by using a thermal camera. Methods: Subjects were 144 eyes of 72 normal university students, who didn't have any corneal disease, abnormal lacrimal ducts, medical records regarding ocular surgeries, or experience of using contact lens. Subjects were divided into two groups, which were normal eye group and dry eye group, based on the results of TBUT, Schirmer I test, and McMonnies test. After categorizing the subjects, the temperature of the subjects' ocular surface and the palpebral conjunctiva were measured and analyzed by using a thermal camera (Cox CX series, Answer co., Korea). Results: In the normal eye group's Central Ar.1, Nasal Ar.2, Temporal Ar.3, Superior Ar.4, Inferior Ar.5, the measured amount of temperature change on each area was $-0.13{\pm}0.08$, $-0.14{\pm}0.08$, $-0.12{\pm}0.08$, $-0.14{\pm}0.08$, $-0.10{\pm}0.09(^{\circ}C/sec)$. The dry eye group's results were $-0.17{\pm}0.08$, $-0.16{\pm}0.07$, $-0.16{\pm}0.08$, $-0.17{\pm}0.09$, $-0.15{\pm}0.08(^{\circ}C/sec)$. When compared with the normal eye group, the values of Ar.1, Ar.3, Ar.5 were significantly different in the dry eye group(p<0.05). The amount of temperature change, which was observed on the palpebral conjunctiva(Ar.1:central, Ar.2: nasal, Ar.3: temporal) of the normal eyes, measured by thermography, was $34.36{\pm}1.12$, $34.17{\pm}1.10$, $34.07{\pm}1.12^{\circ}C$ on each area. Same values taken from the dry eye group was $33.55{\pm}0.94$, $33.43{\pm}0.97$, $33.51{\pm}1.06^{\circ}C$ on each area. The values of Ar.1, taken from the dry eye group, had a significant difference, compared to the values of the normal eye group(p=0.05). Conclusion: The temperature of the ocular surface decreased faster on the dry eyes, compared to the normal eyes. The temperature measured on the palpebral conjunctiva of the dry eyes were also lower than the normal eyes. The temperature changes on the ocular surface, observed with a thermal camera, were objective values to assess the stability of tear films, and might provide useful data for studies related to dry eye syndrome.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.3
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• pp.19-25
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• 2007

It was studied how using soft contact lens multi-purpose solution (MPS), often used for medical treatment, effects the inhibition on cell growth, and how using the MPS demages eye cells, on rabbit eye's corneal epithelium and endothelium tissue. $ReNu^{(R)}$ (Baush & Lomb, USA), Opti-free $express^{(R)}$ (Alcon, USA), Free-sol $plus^{(R)}$ (Hanamedicon, Korea) were used as MPS. After culturing Clone 1-5C-4 cell lines (Human conjunctival cell lines), cell growth inhibition rate was measured by MIT assay. By making Hematoxylin and Eosin stain specimen, the morphology was observed by optical microscope. In the In vivo experiment, 9 white rabbit eyes (18 eyes) were classified into 3 groups. The experiment group is left eyes (9 eyes) of rabbit, and MPS were dropped; however the control group, the right eyes (9 eyes), were only used a saline solution without a preservatives. After the dropping within the period, the cornea surface of rabbit endothelium tissue was observed using scanning electron microscopy (SEM).

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.4
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• pp.525-531
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• 2013

Purpose: To identify the apoptosis caused by Proparacaine hydrochloride (PPC), a topical anesthesia, applied to conjunctival cell lines and determine whether pigallocatechin-gallate (EGCG), has protective effects on. Methods: The conjunctival cell lines were treated with 0.5% of Alcaine$^{(R)}$, 0.5% of PPC and 0.01% of Benzalkonium chloride (BAC) for 15 minutes, respectively in order to investigate the effects of topical anesthesia on cells, and followed by cultured for 12 and 24 hours. The recovery effects were investigated by measuring level of cellular proliferation inhibiting using MTT assay and LDH assay. The conjunctival cell lines were pre-treated with EGCG $10{\mu}M$ for 3 hrs and post-treated with 0.5% PPC for 15 mins in order to investigate whether EGCG has protective effects, flow cytometry were performed in order to observe apoptosis. Results: A result of the additional culture of 12 and 24 hours and again immediately after the treatment for 15 minutes 0.5% of Alcaine$^{(R)}$, 0.5% of PPC, the 0.01% of BAC, cell viability was not increased in all groups (p<0.05). The cell viabilities were higher than in cells 3 hours post-treated with $10{\mu}M$ of EGCG and pre-treated PPC 0.5% (68.2%), compared to cells ($32.2{\pm}2.0%$) treated only with 0.5% of PPC. PPC 0.5% also induced apoptosis in the treated group was reduced by the addition of EGCG. Conclusions: It is considered that the EGCG has cell protective effects when it is added to PPC, a topical anesthesia, by improving cell viability and inhibiting apoptosis.

• Clinical and Experimental Pediatrics
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• v.45 no.3
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• pp.376-382
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• 2002

Purpose : To identify clinical and laboratory features of atypical Kawasaki disease(KD), and to develop criteria for early diagnosis of atypical KD patients. Methods : All patients with KD treated at our hospital from January 1998 to June 2000 were reviewed retrospectively. Results : Among a total of 167 patients, 28(16.8%) were atypical KD of which seven(25%) were infants. Among the five cardinal symptoms, oral mucosal change(96.4%) occurred most frequently, followed by conjuntivitis(57.1%) and rash(46.4%). Most notable laboratory findings were anemia, and increased erythrocyte sedementation rate(ESR) or C-reactive protein(CRP). Coronary artery abnormalities developed in seven(25.8%) atypical KD patients, compared with 14.4% in typical KD patients. We considered oral mucosal change as major criterion, and conjunctivitis, rash, hematocrit <35% and ESR >30 mm/hr or CRP >3.1 mg/dL as minor criteria. Proposed modification in diagnostic criteria for atypical KD include fever of ${\geq}5$ days＋major criterion＋${\geq}2$ minor criteria, or fever of ${\geq}5$ days＋4 minor criteria. Conclusion : The modified diagnostic criteria has yielded a sensitivity 89.3%. Our diagnostic criteria may be used for early diagnosis of atypical KD.

• Journal of Life Science
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• v.14 no.4
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• pp.620-626
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• 2004

Viruses causing acute conjuntivitis were isolated from 675 patients carrying eye infections for year 2001 to 2003 in Busan reagion and their antigenic properties characterized by a serological survey. In 2001, adenoviruses (serotype 8) were found in 5 of 48 cases. In 2002, the isolated viruses were 7 adenoviruses (serotype 8 and 37), 8 coxsakieviruses (serotype A24 and B3) and 1 echoviruses (serotype 6) from 324 specimens that are known as the causative agents of acute hemorrhagic conjuctivitis (AHC). In 2003, 25 case of 303 specimens were 7 adenoviruses (serotype 3, 4, 8 and 37), 7 echoviruses (serotype 6 and 7) and 4 untypable enteroviruses. Although coxsakievirus (serotype B3) and echoviruses (serotype 6 and 7) were generally known as causative agent of aseptic meningitis, it hasn't been reported until now that they were isolated from the conjunctival swabs. The out break of AC was observed from April to October in Busan. These isolated viruses showed a strong cytophatic effects on HEp-2, RD, Vero and BGM cell strains. Analysis of electron micrograph of those viruses showed that adenovirus consists of a 80 nm diameter and nonenvloped icosahedron and then echovirus and coxsackievirus were small nonenveloped and isometric-shaped viruses. Adenovirus showing a cytophatic effect was resulted in a 458 bp single band by PCR and echovirus, coxsackievirus and untypable enterovirus were detected a 437 bp products by RT-PCR.

• 방재와보험
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• s.113
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• pp.70-71
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• 2006

언제부터인가 우리는 도시 공해와 더불어 결코 유쾌하지만은 않은 바다 건너온 손님들에 의해 건강을 위협받고 있다. 바로 중국, 내몽골 지역의 사막화로 인해 겨우내 얼어 붙었던 흙이 봄바람을 만나 생기는 황사가 그 주범이다. 비록 황사가 토지의 산성화를 막아 농작물이 잘 자라는 데 도움을 준다는 말도 있지만, 요재 불어오는 황사는 중국의 산업화에 힘입어 공해물질을 다량 함유한 먼지이기에 더욱 그 피해가 심각하다. 대표적으로 인체의 호흡기에 침입하여 여러 문제를 일으키고 눈이나 피부에도 질환을 일으킬 수 있다. 즉 황사는 눈을 직접 자극하여 결막염의 증세를 일으킬 뿐만 아니라, 함유된 여러 중금속과 같은 물질들에 의해 알레르기성 질환도 유발할 수 있다는 뜻이다.