• Journal of Life Science
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• v.30 no.3
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• pp.313-319
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• 2020

Recently, cattle epidemic diseases are caused by a pathogen such as a virus or bacterium. Such diseases can spread through various pathways, such as feed intake, respiration, and contact between livestock. Diagnosis based on the ELISA (Enzyme-linked immunosorbent assay) and PCR (Polymerase chain reaction) methods has limitations because these traditional diagnostic methods are time consuming assays that require multiple steps and dedicated equipment. In this review, we propose the use of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas system based on DNA and RNA levels for early point-of-care diagnosis in cattle. In the CRISPR/Cas system, Cas effectors are classified into two classes and six subtypes. The Cas effectors included in class 2 are typically Cas9 in type II, Cas12 in type V (Cas12a and Cas12b) and Cas13 in type VI (Cas13a and Cas13b). The CRISPR/Cas system uses reporter molecules that are attached to the ssDNA strands. When the Cas enzyme cuts the ssDNA, these reporters either fluoresce or change color, indicating the presence of a specific disease marker. There are several steps in the development of a CRISPR/Cas system. The first is to select the Cas enzyme depending on DNA or RNA from pathogens (viruses or bacteria). Based on that, the next step is to integrate the optimal amplification, transducing method, and signal reporter. The CRISPR/Cas system is a powerful diagnostic tool using a gene-editing method, which is faster, better, and cheaper than traditional methods. This system could be used for early diagnosis of epidemic cattle diseases and help to control their spread.

• Journal of Bio-Environment Control
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• v.20 no.2
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• pp.93-100
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• 2011

Maintenance of adequate soil water potential during the period of crop growth is necessary to support optimum plant growth and yields. A better understanding of soil water movement within and below the rooting zone can facilitate optimal irrigation scheduling aimed at minimizing the adverse effects of water stress on crop growth and development and the leaching of water below the root zone which can have adverse environmental effects. The objective of this study was to evaluate the feasibility of using a portable irrigation controller with an Watermark sensor for the cultivation of drip-irrigated vegetable crops in a greenhouse. The control capability of the irrigation controller for a soil water potential of -20 kPa was evaluated under summer conditions by cultivating 45-day-old tomato plants grown in three differently textured soils (sandy loam, loam, and loamy sands). Water contents through each soil profile were continuously monitored using three Sentek probes, each consisting of three capacitance sensors at 10, 20, and 30 cm depths. Even though a repeatable cycling of soil water potential occurred for the potential treatment, the lower limit of the Watermark (about 0 kPa) obtained in this study presented a limitation of using the Watermark sensor for optimal irrigation of tomato plants where -20 kPa was used as a point for triggering irrigations. This problem might be related to the slow response time and inadequate soil-sensor interface of the Watermark sensor as compared to a porous and ceramic cup-based tensiometer with a sensitive pressure transducer. In addition, the irrigation time of 50 to 60 min at each of the irrigation operation gave a rapid drop of the potential to zero, resulting in over irrigation of tomatoes. There were differences in water content among the three different soil types under the variable rate irrigation, showing a range of water contents of 16 to 24%, 17 to 28%, and 24 to 32% for loamy sand, sandy loam, and loam soils, respectively. The greatest rate increase in water content was observed in the top of 10 cm depth of sandy loam soil within almost 60 min from the start of irrigation.

• Journal of Sericultural and Entomological Science
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• v.17 no.2
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• pp.141-149
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• 1975

Silkworm is known to be damaged under the unfavorable environmental conditions. Recently, it has been often argued that nicotine is a main factor to affect the silkworm nutritional balance and to result in decrease of quantitative characters, and unbalance of larval nutrition. Especially in spring season, there is tendency to produce nicotine damage to silkworm fed with the mulberry leaf near to tobacco field early cultivated in mulching system. In these connections, the trial was made in the different pots placing by the different distance 10rn, 20m, 40m, and 60m in the direction of east-north from the tobacco field. Location of pot near to the tobacco fold is 40m below the tobacco Held and gradually higher than the tobacco field by 10 degree of slope. In addition, when the mulberry field is divided into two areas from the east-south to west-north, southern east zone is lowering down below 10 degree of slope and has 36％ of east-south wind, 21％ of south-east wind and 16％ of south wind, etc. Pots are left on the mulberry field from the completion of lower part leaf harvest to just before upper part leaf harvest is completed for 32 days. Then silkworm at 4th-5th stage was fed with the leaf grown during the period and larval characters are statistically analyzed. The results are summarized as follows： 1. 10-40m distant pot statistically at the same level has the longest larval duration and 60m distant pot is. not significant at 5％ level. Whole larval duration has same tendency as in the 5th larval duration. 2. Percentage of delayed molting larvae at the 5th stage is increased in 10m distant pot and there is no significance statistically in other pots, as compared with that of control. 3. It is not showed that there is no damage to mortality of the old larvae in 60m distant pot. 4. 10-20m distant pot produces a low pupation and others are at the same level statistically at 5％ significance, 5. It is showed that nicotine damage is occurred in single cocoon freight and cocoon shell weight up to 60m distant pot, rather higher damage in cocoon shell weight than in single cocoon weight. 6. It is showed that cocoon yield from 10, 000 molted larvae including double cocoon is decreased up to 40m distant pot far from the tobacco field in which 40％ of cocoon yield in 10m distant pot by 7kg, 26％ of cocoon yield in 20m distant pot by 3.9kg, 24％ of cocoon yield in 40m distant pot by 3.6kg, and 12％ of cocoon yield in 60m distant pot by 1.8kg, as compared with control. However, there is no significance statistically at 5％ level in 60m distant pot. With these above results, it is concluded that nicotine da mage could be occurred in cocoon yield and cocoon quality up to 60m distant far from the tobacco field.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.349-355
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• 2022

In this study, we sought to identify a soil stabilizer that can be applied to paddy fields vulnerable to arsenic (As) pollution. To this end, we conducted a pot experiment in which we evaluated the effects of different stabilizers on the bioavailability of As in paddy soil. As candidate stabilizers, we assessed calcium superphosphate (CSP), sulfur, and steel slag, which were applied at the rates of 0.7 and 1.4, 0.1 and 0.2, and 7.0 and 14.0 Ma ha^-1, respectively. On day 67 after rice transplantation, we detected significantly lower concentrations of As in the solutions of paddy soil treated with 1.4 Ma ha^-1 CSP (96.9 ㎍ L^-1) and 0.2 Ma ha^-1 sulfur (207.2 ㎍ L^-1) compared with the As concentrations in control (314.5 ㎍ L^-1) and steel slag-treated (268.6-342.4 ㎍ L^-1) soil. Compared with the As concentrations in control brown rice (0.16 mg kg^-1), concentrations in brown rice were lowest in the pots treated with 1.4 Ma ha^-1 CSP (0.09 mg kg^-1). Furthermore, in response to CSP treatments, we detected increases in the weight of rice grains (50.0-50.4 g/pot) compared with the control (40.4 g/pot) and other treatments (26.9-48.1 g/pot), which we speculate could be attributed to the reduction in As toxicity to rice owing to a decline in soil solution As contents and the fertilization effect of the CSP treatment. Collectively, our findings indicate a high-level application of CSP (1.4 Ma ha^-1) to paddy soil has a comparatively beneficial effect in mitigating the bioavailability of As.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.591-597
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• 2015

We determined the total C and N stocks in trees and soils after 1 year of fertilization in an experimental orchard with 16-year-old 'Niitaka' pear (Pyrus pyrifolia Nakai cv. Niitaka) trees planted at $5.0m{\times}3.0m$ spacing on a Tatura trellis system. Pear trees were fertilized at the rate of 200 kg N, 130 kg P and $180kg\;K\;ha^{-1}$. At the sampling time (August 2013), trees were uprooted, separated into six fractions [trunk, main branches, lateral branches (including shoots), leaves, fruit, and roots] and analyzed for their total C and N concentrations and dry masses. Soil samples were collected from 0 to 0.6 m in 0.1 m intervals at 0.5 m from the trunk, air-dried, passed through a 2-mm sieve, and analyzed for total C and N concentrations. Undisturbed soil core samples were also taken to determine the bulk density. Dry mass per tree was 5.6 kg for trunk, 12.0 kg f or m ain branches, 15.7 kg for lateral branches, 5.7 kg for leaves, 9.8 kg for fruits, and 10.5 kg for roots. Total amounts of C and N per tree were respectively 2.6 and 0.02 kg for trunk, 5.5 and 0.04 kg for main branches, 7.2 and 0.07 kg for lateral branches, 2.6 and 0.11 kg for leaves, 4.0 and 0.03 kg for fruit, and 4.8 and 0.05 kg for roots. Carbon and N stocks stored in the soil per hectare were 155.7 and 14.0 Mg, respectively, while those contained in pear trees were 17.8 and $0.2Mg{\cdot}ha^{-1}$ based on a tree density of 667 trees/ha. Overall, C and N stocks per hectare stored in the pear orchard were 173.6 and 14.2 Mg, respectively. Compared with results obtained in 2012, the amounts of C stocks have increased by $17.7Mg{\cdot}ha^{-1}$, while those of N stocks remained virtually unchanged ($0.66Mg{\cdot}ha^{-1}$).

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.289-296
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• 2001

The main objective of this topic is to establish strategies and to plan biotechnology researches which are related to the agricultural improvements especially focusing on the crop breeding in Korea. From 1960's to 1980's government policy had been emphasized to develope high yielding cultivars for the self sufficient supply of the staple food crops. As a result, considerable increase of rice production has been made with accumulating technology and man's powers. Recently genetically modified crops harboring useful characteristics have been developed using biotechnology and released in the developed countries. National research institutes and private companies have been developed biotechnology researches to establish competitive capabilities, however they have not been successfully used in commercialization. Therefore it is necessary to promote the practical. application by connecting molecular technology with conventional breeding. Proposed research projects are; (1) basic researches including plant genome studies, (2) developing new cultivars through gene transformation, (3) screening and producing antioxidants, secondary metabolite substances and edible vaccines. To set a government policy, both domestic and international research trends were reviewed and possibility of success based on the economic view point were discussed. The intellectual property and preservation of environment play a key role to decide the research priority. It is also necessary for us to make one step system for the distribution of research resources such as microorganisms, genes cloned, plant seeds and research informations for promoting research activities.

• Korea journal of population studies
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• v.30 no.2
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• pp.71-95
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• 2007

This paper attempts to identify the principle of family succession and family patterns of yangban in the late Chosun period through an analysis of male adaptation cases found in family registration records. The primary source of analysis is the family registration documents of Uiseong Kim's from the late 17th century to the early 20th century. As a result, it is found that there is a substantial change in the patterns of family from the early and mid Chosun period to the late Chosun period. The change is the strengthening of the principle of patriarchy succession through male adoption. Looking at the data as a whole, the average number of household members is increased and the membership of kinship also expanded. In contrast to the family patterns of the early Chosun period, not only the patterns of Uiseong Kim's family are predominately immediate family or collateral family but also the majority is extended family in the 18th and 19th centuries. The male adoption cases recorded in Uiseong Kim's family registration documents take up 33.8% of the male adoption cases in the entire family registration documents. This goes to show that the strengthening of the principle of primogeniture succession at a time when child mortality rate is very high resulted in the increase of male adoption. In conclusion, the late Chosun society was a society where the seat of primogeniture was much more important than immediate hereditary members in the family succession.

• Archives of design research
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• v.17 no.1
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• pp.77-88
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• 2004

It was 1988 when people pay attention to an importance of the facilities for the handicapped, with Seoul Paralympics. In early 1990s, regular efforts were made to improve a physical environment for the handicapped, which results in reforming the Welfare Law for the Handicapped all over the surface. However, keeping pace with the expansion of the western universal design concept, it is required to evaluate a new concept in which way the facilities for the handicapped must be installed. That is, any physical environment, which regards the handicapped as an independent subject, tend to isolate them from normal people rather than satisfy their needs. making the handicapped feel more discriminated. All the facilities for the handicapped has to reflect a general idea that the facilities are opened for all the people including the handicapped. Being recently reformed and enacted in order to reflect this essential point, 'the raw of contributing to the convenience of the handicapped, aged men, pregnant women and nursing mother(1998)' even covers the aged men, pregnant women and nursing mother, not to mention of the handicapped. In addition, the law states clearly that all the handicapped can share all the facilities with the normal people, inducing the handicapped to more actively participate in society. Consequently, the handicapped are acquiring their rights by directly demanding for correction of varied discrimination. Though even more facilities have been provided, most of them are deficient in the quality, setting limits to the handicapped person's living field as well as social activities. Especially, most public buildings in our country rarely provide the adequate facilities for the handicapped, aged men, pregnant women and nursing mother. Therefore, this study is focused on developing the list of actual condition research that can effectively detect the inadequacy of facilities in public buildings. Then it will find the aspects to be improved in a systematic and scientific way to propose a practical method to improve the facilities in public buildings.