• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.8
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• pp.1009-1017
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• 2008

For the effective use of exopeptidase from squid viscera as food processing aids, the viscera of Argentina shortfin squid (Illex argentinus) were fractionated by various methods such as acetone treatment, ammonium sulfate treatment, anion exchange chromatography, and gel filtration. The positive exopeptidase fractions were obtained from the fraction II treated by cold acetone ($30{\sim}40%$, w/w), the fraction V by ammonium sulfate ($60{\sim}70%$ saturation), the fraction II (0.2 M NaCl) by anion exchange chromatography, and the fraction I ($30{\sim}50\;kDa$) by gel filtration. The specific activities of positive fractions from viscera of I llex argentinus against substrates were higher to LeuPNA than to ArgPNA. Total activity and recovery against LeuPNA of positive fraction by gel filtration were 1,867 U and 30.69%, respectively, which were the highest among those of positive fraction. The results suggested that the gel filtration chromatography method was the most efficient method for the fractionation of exopeptidase from viscera of Illex argentinus.

• Applied Biological Chemistry
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• v.33 no.1
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• pp.34-38
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• 1990

The molecular structural properties of amylopectins of Chunmabyeo(Japonica), Yongmunbyeo($Indica{\times}Japonica$) and Mahatma(Indica) rice were investigated. The intrinsic viscosity of Chunmabyeo, Yongmunbyeo and Mahatma amylopectin were 148.3 ml/g, 156.6 ml/g and 167.5 ml/g, and ${\beta}-amylolysis$ limit(%) were 54.6, 55.4 and 52.9 respectively. Average unit chain length(${\overline{CL}}$) and average inner chain length(${\overline{ICL}}$) of Mahatma amylopectin were longer than other varieties. Elution profiles by Sephadex G-50 chromatography of debranched amylopectins with ${\beta}-amylase$ showed two peaks (void volume, ${\overline{DP}}$3) and the elution profiles of debranched amylopectins with pullulanase showed three peaks(void volume, ${\overline{DP}}$35-45, ${\overline{DP}}$10-20). The ratio of Peak III(${\overline{DP}}$10-20) to Peak II (${\overline{DP}}$ 35-45) of Chunmbyeo, Yongmunbyeo and Mahatma amylopectin were 3.9, 3.4 and 3.3, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.198-204
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• 2000

In order to utilize by-products which would normally be discarded in marine processing plants, cod teiset protein was hydrolyzed and antioxidative actiTity of the hydrolysate was investigated. AntioxidatiTe peptide was isolated using ultrafiltration membrane, ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, high performance liquid chromatography on an ODS column, and capillary electrophoresis chromatography. Antioxidative activities of the cod teiset hydrolysate were compared with ${\alpha}-tocopherol$, one of the commercial antioxidant. The hydrolysate passed through a membrane with molecular weight cut-off (MWCO) 1 kDa was shown the strongest antioxidative activity, and the activity was higher $10{\%}$ as compared with ${\alpha}-tocopherol$. In addition, the peptide isolated by ion-exchange chromatography, gel filtration, and HPLC, respectively, was higher $53{\%}$ as compared with ${\alpha}-tocopherol$, and the amino acid sequence was Ser-Asn-Pro-Glu-Trp-Ser-Trp-Asn.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.199-203
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• 2016

Apolipophorin-III (apoLp-III) was isolated and purified from the last larval hemolymph of Galleria mellonella by KBr gradient ultracentrifugation and gel chromatography (Sephadex G-100). After KBr gradient ultracentrifugation, the lipophorin-free fractions were used as the samples for gel chromatography. The purity of the finally purified apoLp-III was confirmed by SDS-PAGE after gel chromatography. In this study, we found that apoLp-III is taken up into the adult testes in Galleria mellonella. The testes were dissected from day-1 or -2 adults in cold Ringer's solution and used for tissue culture. The protein moiety of apoLp-III was labeled with FITC dissolved in dimethyl sulfoxide (DMSO) at room temperature under conditions of continuous stirring for 1 h. The FITC-labeled apoLp-III was purified with a Sephadex G-25 PD-10 column. The tissues of the adult testes were incubated at room temperature for 30 min with fluorescein isothiocyanate (FITC)-labeled apoLp-III. Fluorescein microscopy and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed that the adult testis tissues internalize the FITC-labeled apoLp-III. The results showed that apoLp-III is taken up by the adult testes.

• KSBB Journal
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• v.9 no.1
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• pp.55-62
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• 1994

Glucose oxidase(EC 1.1.3.4) was purified to electrophoretic homogeneity from Aspergillus niger by a combination of ammonium sulfate fractionation, ion exchange chromatography, and ultrafiltration. Two active fractions A and B, of glucose oxidase were obtained from the hydrophobic chromatography on phenyl sepharose CL-4B. The enzyme A and B were glycoproteins with the same denatured molecular weight of 78, 000 and had specific activities of 2, 191 and 1, 273-units/mg proteins, respectively. But the two enzymes showed differences in native molecular weight that was measured by HPLC gel filteration, maximum absorbtion wavelength and isoelectric point. The enzyme A oxidized $\beta$-D-glucose only and was resistant to sodium dodecyl sulfate. Activity optimum was found at $30^{\circ}C$ and pH 3.5. Also the enzyme A was inhibited greatly by $Hg^{2+}$(10mM). The results of chemical modification experiments suggested that cysteine and cystine residues might be involved in the active site of the enzyme A.

• Journal of the Korean Chemical Society
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• v.42 no.2
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• pp.190-196
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• 1998

A new sampling and size exclusion chromatography (SEC) method for the analysis of underivatized cellulose are established. In this method, cellulose materials are first dissolved in N-methylmorpholine N-oxide (NMMO) and diluted by adding dimethyl sulfoxide (DMSO) to make the sample solutions of about 0.1% in 50/50 NMMO/DMSO (w/w). Sample solutions are analyzed using a glucose-treated divinylbenzene (DVB) SEC column and DMSO containing 0.05M LiBr and 2.5 blank as the eluant. The flow rate was constant at 1 mL/min and the whole SEC system including the column was heated at $80^{\circ}C$ to reduce the viscosity of DMSO. Addition of 0.05 M LiBr eliminated SEC baseline drifting, and addition of 2.5 blank seems to reduce the interaction between the sample and the column packing. SEC molecular weights were determined using a calibration curve constructed from a series of narrow pullulan standards, and they were used to measure the degree of degradation during two different pulp-to-sponge processings.

• Applied Biological Chemistry
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• v.47 no.1
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• pp.22-26
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• 2004

The ligninolytic basidiomycete, Pleurotus ostreatus K-2946, was produced a manganese peroxidase (MnP) activity when grown in liquid culture with glucose-yeast-peptone (G-Y-P) medium. However, lignin peroxidase (LiP) was not detected in this culture medium. The purification progress of MnP was purified that included chromatography on Sepharose CL-6B, Superdex 75 prep grade and Mono-Q. MnP purified by column chromatography, was 36400 dalton and a pI of 3.95. The optimal pH and temperature of the purified MnP activity were 5.0 and $55^{\circ}C$. The characteristics of MnP produced was quite similar to those of MnP 3 isoenzyme produced by other strains of P. ostreatus.

• Korean Journal of Food Science and Technology
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• v.24 no.6
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• pp.568-573
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• 1992

Molecular structural properties of Japonica and Tongil type waxy rice starches were compared. The general properties including water-binding capacity, swelling power at $80^{\circ}C$, intrinsic viscosity, leach loss at $98^{\circ}C$ and ${\beta}-amylolysis$ limit were similar but the chain length was slightly different between the two starches. Similar elution profiles on Sephadex G-50 were obtained when the starches were treated with ${\beta}-amylase$. The pullulanase treated starches however showed different elution patterns each other. The ${\beta}-amylolysis$ of acid-treated (2 days) starches linearly increased as hydrolysis time prolonged. When the acid-treated starches were hydrolyzed with ${\beta}-amylase$ or pullulanase, the elution profiles on Sepharose CL-2B were considerably different from those of native starches.

• The Korean Journal of Food And Nutrition
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• v.9 no.4
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• pp.430-433
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• 1996

The activity of PAL, an initial and key regulatory enzyme in biosynthesis of phenolics, peaked during ripening stage. The enzyme activities were 5.60 and 4.25 units/100g fr. wt. in ripening and overripening stages. The increase in PAL activity in ripening strawberry fruits is due to de novo synthesis of the enzyme. PAL extracts from ripe strawberry fruits have a native molecular weight of about 260, 000 Da.

• Journal of the Korean Society of Clothing and Textiles
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• v.26 no.7
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• pp.1078-1084
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• 2002

Cotton fabrics were treated with 1,2,3,4-butanetetracarboxylic acid(BTCA) to impart durable press performance, which is formaldehyde-free DP finishing reagent. The pore structures of BTCA treated cottons were compared using a reverse gel permeation chromatographic technique(reverse GPC). A series consisting 4 kinds of water soluble sugars was used to study the elution characteristics of columns prepared from cotton fibers. From these data, differences in pore size distribution in the control and BTCA treated cottons were distinguished. BTCA crosslinks cellulose molecules provided wrinkle resistance to the treated cotton fabrics through ester linkages. Although crosslinking of cotton with BTCA reduced accessible internal volume across the entire range of pore size, differences in line pores were larger than in small pores. BTCA treated cotton exhibited reductions over 40% in large pore sizes.