• Title/Summary/Keyword: 건조층

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Establishment of In Vitro 3-Dimensional Culture System of Mouse Endometrial Cells;I. Cytohistological Study on Mouse Endometrium (마우스 자궁내막 세포를 이용한 3차원적 배양시스템 확립에 관한 연구; I. 마우스 자궁내막에 관한 세포조직학적 연구)

  • Nam, Hwa-Kyung;Kim, Eun-Young;Lee, Keum-Sil;Park, Sae-Young;Park, Eun-Mi;Kwon, Jung-Kyun;Yoon, San-Hyun;Park, Se-Pil;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.1
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    • pp.31-37
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    • 2000
  • This study was designed to identify the ultrastructural changes of mouse endometrium during peri-implantation period and obtain the fundamental information for the establishment of 3-dimensional culture system of mouse endometrial cells in vitro. The used female ICR mice ($6{\sim}8$ wks) were conducted on pregnant. The biopsies were obtained from whole uterus at cycle day 1 (D1) and day 5 (D5) after hCG injection and mating. The biopsies materials were fixed 2.5% glutaraldehyde and 1% osmium tetroxide. Subsequently, for observation using light and transmission electron microscopy (LM and TEM), they were dehydrated and embedded in Epon and the embedded biopsies were sectioned and stained. For scanning electron microscopy (SEM), the fixed specimens were dehydrated, dried and coated with gold. 1) For LM, the biopsied materials at D5 (late secretory phase) were appeared the extended stromal layer by increased connective tissues and the fully developed endometrial glands and vessels compared with D1 (early secretory phase). 2) For TEM, the mouse endometrium was consisted of 3-layers, a simple polarized columnar epithelial cells, basement membrane and stromal cells. At D5, the distribution of microvilli, endoplasmic reticulum, Golgi body, lipid and glycogen deposits, secretory granules and surface area of basement membrane were increased. 3) For SEM, the degree of folding and microvilli of surface of mouse epithelial cells was became more and more according to the process of secretory phase, and at D5, implantation time of mouse, the appearance of pinopodes as a specific marker of uterine receptivity was found. The uterine pinopodes of mouse were found in narrow sites at the luminal surface, irregularity and appeared the different stages in the same sample. Therefore, these results indicated that the mouse endometrium was experienced dramatic morphological changes during peri-implantation period.

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Immobilization and Recycling of Arsenic-Contaminated Fine Soil Cake Produced after Soil Washing Process (토양세척 후 발생하는 비소오염 탈수미세토의 불용화 및 재활용 평가)

  • Oh, Minah;Moon, SoYoung;Hyun, Min;Chae, HeeHoon;Lee, Jai-Young
    • Journal of the Korean Geosynthetics Society
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    • v.11 no.4
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    • pp.9-16
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    • 2012
  • Standardized remediation process for the soil contaminated with arsenic is insufficient due to characteristics of its anion-mobility and speciation changed by Eh-pH of soil. One of the well-known efficient remediation processes is the modified soil washing that particle separation process by only water. However, it is required that the treatment plan for the fine soil what was discharged after modified soil washing. Therefore, this research suggests the treatment plan that the recycling method using arsenic immobilization by FeS-$H_2O_2$. The batch experiments results for the arsenic immobilization showed that the water content was at least 50%, the injection of FeS and $H_2O_2$ (assay-35%) were 8% (w/watdrybase) and 0.2 mL/10 g of fine soil respectively. Arsenic concentration with KSLT was decreased about 95.4%. The results indicated that the mixing of FeS-$H_2O_2$ was highly efficient on the immobilization of As-contaminated soil. The mixing ratio as 13% of bentonite with 3% of cement (at based on 100% of immobilized fine soil) was satisfied with standard of liner for landfill construction.

Analysis of Seed Storage Data and Longevity for Agastache rugosa (배초향 (Agastache rugosa) 종자의 저장 반응과 수명 분석)

  • Lee, Mi Hyun;Hong, Sun Hee;Na, Chae Sun;Kim, Jeong Gyu;Kim, Tae Wan;Lee, Yong Ho
    • Korean Journal of Environmental Biology
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    • v.35 no.2
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    • pp.207-214
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    • 2017
  • There is little information about the seed longevity of wild plants, although seed bank storage is an important tool for biodiversity conservation. This study was conducted to predict the seed viability equation of Agastache rugosa. The A. rugosa seeds were stored at moisture contents ranging from 2.7 to 12.5%, and temperatures between 10 and $50^{\circ}C$. Viability data were fitted to the seed viability equation in a one step and two step approach. The A. rugosa seeds showed orthodox seed storage behaviour. The viability constants were $K_E=6.9297$, $C_W=4.2551$ $C_H=0.0329$, and $C_Q=0.00048$. The P85 of A. rugosa seeds was predicted to 152 years under standard seed bank conditions. The P85 predicted by seed viability equation can be used as basic information for optimization of seed storage processes.

A STUDY ON THE EFFECT OF SALIVA CONTAMINATION ON THE BOND STRENGTH OF ONE-BOTTLE ADHESIVE SYSTEM (타액 오염이 One bottle 상아질 결합제의 전단결합강도에 미치는 영향에 관한 연구)

  • Jeon, Hyung-Joon;Kim, Jong-Soo;Kwon, Soon-Won
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.4
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    • pp.566-574
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    • 2001
  • For decades it has been a clinically accepted requirement, in case of salivary contamination, to re-etch conditioned enamel and dentin to proceed with the adhesive technique. Only a few reports have been so far dealing with the potential of one-bottle adhesive system to bond even when applied after salivary contamination and without re-etching. The purpose of this study was to evaluate the influence of the salivary contamination on the shear bond strength of dentin. The results were as follows: 1. In group II, in which saliva contamination and washing occurred before applying of Prime & Bond NT showed high shear bond strength as in control group. In Group III and IV, in which the cured adhesive was contaminated with saliva, showed significantly lower mean bond strength, 2. Relating long resin tags of $70\sim120{\mu}m$ were observed in samples of all groups under SEM. We could observed hybrid layer, resin tag and many lateral branches in every group. And there were no differences between groups.

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Conservation Treatment on the Bamboo Sunblind from the No. 1 Catchment Site in Baesanseongji, Busan (부산 배산성지 1호 집수지 출토 대나무 발 수습 및 보존처리)

  • Park, Ji Hyeon;Park, Jung Hae;Lee, Kwang Hee;Seo, Yeon Ju;Park, Jung Wook;Kim, Soo Chul
    • Journal of Conservation Science
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    • v.37 no.5
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    • pp.536-544
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    • 2021
  • In the present study, safe management and value improvement of bamboo sunblind, which is an item of cultural heritage, were performed by adopting stable conservation treatment methods. The bamboo sunblind used in the present study was excavated from No. 1 catchment site in Baesanseongji, Busan. It was determined that the main material used to make the sunblind was bamboo, and herbal plants were used to weave the bamboo using lacquer as an adhesive agent. All contaminants and soil adhered to the sunblind was removed. Thereafter, the sunblind, which was recovered in the form of blocks, was washed separately after fixing it to a temporary plaster frame and to avoid the blocks from breaking during washing. Then, polyethylene glycol (PEG) impregnation was utilized for the reinforcement treatment. Based on the preliminary test results, the shape of the sunblind was fixed using a stainless-steel frame to prevent physical damage that may occur during the drying process. Thereafter, the bamboo sunblind was vacuum freeze-dried. PEG 20% (in ethyl alcohol) was applied as a surface treatment agent for stabilization the sunblind. After the surface treatment, the bamboo sunblind were joined together to fit the maximum width, and the rectangular shape of the sunblind was restored-as best as possible-while filling in the missing parts by maximizing the use of unknown members such as in the disturbed layers below bamboo sunblind surface. The conservation treatment was completed by fixing the bamboo sunblind into the fabricated frame.

Morphological Landscape of Patagonia and Atacama in the Andes based on Geotourism (지오투어리즘 관점에서 본 안데스 파타고니아와 아타카마의 지형경관)

  • PARK, Jongkwan
    • Journal of The Geomorphological Association of Korea
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    • v.20 no.4
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    • pp.15-28
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    • 2013
  • This paper was made on the basis of geotourism to explain morphological characteristics of Patagonia and Atacama region in the Andes through 10-day fieldwork. The Patagonian landscape was focused on the Torres del Paine National Park, Chile and the Los Glacier National Park, Argentina; on the other hand, the Atacama morphological landscape was investigated around the San Pedro de Atacama. Many geomorphological features such as uplift, semiarid and glacier landscapes can be found in Patagonia, so it is strongly recommended to have an opportunity to enjoy these landforms by geotourism. Massive intrusive rock bodies of granite can be shown in those two National Parks declared a Biosphere Reserve by UNESCO. And, the Atacama Desert located on the forearc depression in the Andes, Chile has also many geomorphological destinations of arid and volcanic landforms; badland, pediplain, pediment, salt lake and geyser. Especially, huge pediplain is one of the greatest view points in the Atacama Desert. Patagonia and Atacama are the best geographical tourism sites to know diverse tectonic landforms made by the conflict between South American and Pacific plates.

Analysis of Holdup Characteristics of Large and Small Bubbles in Three-Phase Fluidized Beds by using a Dynamic Gas Disengagement Method (삼상유동층에서 동력학적 기체유출 측정방법에 의한 큰 기포와 작은 기포의 체류량 특성 해석)

  • Lim, Hyun Oh;Lim, Dae Ho;Seo, Myung Jae;Kang, Yong;Jung, Heon;Lee, Ho Tae
    • Korean Chemical Engineering Research
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    • v.49 no.5
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    • pp.605-610
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    • 2011
  • Phase holdup characteristics of relatively large and small bubbles were investigated in a three-phase(gasliquid-solid) fluidized bed of which diameter was 0.105 m(ID) and 2.5 m in height, respectively. Effects of gas(0.01~0.07 m/s) and liquid velocities(0.01~0.07 m/s) and particle size($0.5{\sim}3.0{\times}10^{-3}m$) on the holdups of relatively large and small bubbles were determined. The holdups of two kinds of bubbles in three phase fluidized beds were estimated by means of static pressure drop method with the knowledge of pressure drops corresponding to each kind of bubble, respectively, which were obtained by dynamic gas disengagement method. Dried and filtered air which was regulated by gas regulator, tap water and glass bead of which density was $2500kg/m^3$ were served as a gas, a liquid and a fluidized solid phase, respectively. The two kinds of bubbles in three-phase fluidized beds, relatively large and small bubbles, were effectively detected and distinguished by measuring the pressure drop variation after stopping the gas and liquid flow into the column as a step function: The increase slope of pressure drop with a variation of elapsed time was quite different from each other. It was found that the holdup of relatively large bubbles increased with increasing gas velocity but decreased with liquid velocity. However, the holdup showed a local minimum with a variation of size of fluidized solid particles. The holdup of relatively small bubbles increased with an increase in the gas velocity or solid particle size, while it decreased slightly with an increase in the liquid velocity. The holdups of two kinds of bubbles were well correlated in terms of operating variables within this experimental conditions, respectively.

A Study of collapsed conditions of the stone pagoda in Mireuk Temple Site (미륵사지석탑 붕괴상태 고찰)

  • Kim, Derk-Moon
    • Korean Journal of Heritage: History & Science
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    • v.38
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    • pp.305-327
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    • 2005
  • Although the stone pagoda in Mireuk Temple site, Iksan, Cholla Province has been collapsed long time ago, few historical record has clearly explained the reason why the pagoda was collapsed and when. The west side of the pagoda have been destroyed from top to the sixth floor and the broken or damaged stone materials have been piled up in disorder. the lower part in the west was reinforced and enclosed by a stone embankment levelled to the height of the first storey of the pagoda. With no record informing the historical fact when it was made and by whom, it is only presumed that the embankment may have been built long time ago in order to prevent remains from further destruction. In the second chapter of the study, it has been tried to restore a reasonable historical background of the pagoda based on records or comments found in literatures such as traditional poetry and essays in chronological order. The collapsed slope in the west side, just above the embankment surrounding the lower part of the pagoda, was concreted in 1915 during the Japanese colonial period. Then in 1998, the Jeollabukdo has examined the structural safety of the pagoda. The Cultural Properties Committee has decided have the concrete layer removed and moreover to take apart the whole pagoda. It is also included that the disassembled stone materials should be given proper conservation treatments before being put into the place where they were in the reassembling process. The front view of the collapsed phase of the pagoda was revealed when the concrete-covered layer was removed. A hypothesis was built that there may be as many different appearances of collapsed pagoda depending on natural causes such as earthquake, sunken foundation, flood and typhoon. In chapter three, characteristic features were classified by examining various images of pagodas destroyed by different natural reasons mentioned in historical records. The chapter four dealt with comparison and analysis on the conditions shown in the stone pagoda in Mireuk Temple site and other examples studied in advance. The result of the study revealed that though having been made higher than the ground surface, the podium or the base of the pagoda actually has been eroded by rain and water. The erosion is supposed not only to have been proceeded for a long time without break but also to have caused the first storey body stone in the west inclined to outward. It has come to a conclusion that the pagoda may have been lead to collapse when the first storey body stone, supporting the whole weight from the upper storeys, became out of upright position and lost its balance. However, no such distinctive features of structural changes shown in pagodas collapsed by natural causes like earthquake, typhoon or sunken basement, have been found in the stone pagoda in Mireuk Temple site.

Taxonomical Classification and Genesis of Dongsong Series Distributed on the Lava Plain in Cheolweon (철원 용암류대지 토양인 동송통의 분류 및 생성)

  • Song, Kwan-Cheol;Hyun, Byung-Geun;Sonn, Yeon-Kyu;Zhang, Yong-Seon;Park, Chan-Won;Jang, Byoung-Choon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.217-223
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    • 2010
  • This study was conducted to reclassify Dongsong series based on the second edition of Soil Taxonomy and to discuss the formation of Dongsong series distributed on the lava plain at Cheolweon in Korea. Morphological properties of typifying pedon of Dongsong series were investigated, and physico-chemical properties were analyzed according to Soil Survey Laboratory Methods Manual. The typifying pedon of Dongsong series has brown (7.5YR 4/2) silty clay loam Ap horizon (0-16 cm), brown (7.5YR 4/2) silty clay loam BA horizon (16-22 cm), brown (7.5YR 4/2) silty clay Bt1 horizon (22-50 cm), reddish brown (5YR 5/4) silty clay Bt2 horizon (50-92 cm), and brown (7.5YR 4/3) silty clay loam Bt3 horizon (92-120 cm). It occurs on lava plain derived from baslt materials. The typifying pedon has higher bulk density than 0.90 Mg $m^{-3}$. That can not be classified as Andisol. But it has an argillic horizon from a depth of 22 to more than 120 cm, and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. It can be classified as Ultisol, not as Andisol or Alfisol. It has aquic conditions for some time in normal years in one or more horizons within 50 cm of the mineral soil surface, redoximorphic features between a depth of 25 cm, and a depth of 40 cm from the mineral soil surface, and redox concentrations, and 50%or more redox depletions with chroma of 2 or less in the matrix within the upper 12.5 cm of the argillic horizon. Therefore it can be classified as Aquult. It has episaturation, and keys out as Epiaquult. It has 50% or more chroma of 3 or more in one or more horizons between a depth of 25 cm from the mineral soil surface, and a depth of 75 cm. It can be classified as Aeric Aquult. Dongsong series have 35%or more clay at the particle-size control section, and have mesic soil temperature regime. Therefore they can be classified as fine, mesic family of Aeric Epiaquults, not as fine, mesic family of Typic Epiaqualfs. The Quarternary volcanic activities occurred in Jeju Island, Ulrung Island, Baekryeong Island, Cheolweon area, and Mt. Paekdu et al. in the Korean Penninsula. Most of them belong to the central eruption type, but Cheolweon area may be of the fissure eruption type. Dongsong series occur on Cheolweon lava plains derived from basaltic materials. Most soils distributed in Jeju Island, and derived from mainly pyroclastics are developed as Andisols. But Dongsong series distributed in Cheolweon lava plains which have a relatively dry climate and derived from basaltic materials are developed as Ultisols.

Synecology of the Forest Vegetation of Yeongjongdo (영종도 삼림식생의 군락생태)

  • 이호준;김종홍;전영문;정흥락
    • The Korean Journal of Ecology
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    • v.26 no.5
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    • pp.223-236
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    • 2003
  • The vegetation in this study area was divided into 10 plant communities: Pinus thunbergii, P. densiflora, Quercus acutissima, Q. mongolica, Q. serratea, Q. variabilis, Q. aliena, Q. dentata, Carpinus coreana, Betula davurica and 3 afforestations; Pinus rigida, Robinia pseudo-acacia, Castanea crenata. The dominant species in each stratum as determined by R-NCD appeared to be Q. acutissima, P. densiflora, Platycarya strobilacea, Sorbus alnifolia and Q. aliena in the tree-2 layer, Rhododendron mucronulatum, P. strobilacea, Q. serrata, Q. aliena and Q. acutissima in the shrub layer, and Carex lanceolata, C. humilis, R. mucronulatum, Mischanthus sinensis var. purpurascens and Oplismenus undulatifolius in the herb layer. The distribution of diameter at breast height(DBH) showed the highest percentage (56%) in the range of 2∼10㎝ in the study area and it was investigated as being in the restoration phase after disturbance. The average of the age of trees was under 40-years-od. The pH of soil collected at each site appeared to be within the range between 4.53 and 5.10(the average of 4.73) in each community, organic matters appeared higher than the value in Q. mongolica, P. rigida, C. crenata communities compared to P. thunbergii, P. densiflora communities. And soil organic matters and total nitrogen were highly correlated with their contents.