• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.3
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• pp.126-144
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• 1974

Adult female prawn, Macrobrachium japonicum, which used for this work were collected at the river Simanto, Shikoku in Japan, and removed in the laboratory. Newly hatched larvae from an adult reared in circulation-filtered aquaria, which is controlled under the conditions of water temperature $26\pm0.3^{\circ}C$, chlorinity $6.21-6.45\%$ Cl, pH 8.0-8.1, illumination 3000 lux, and fed on Artemia salina nauplius sufficielltly For a period of rearing of zoea larvae, big individuals-individual varying bodies, comparing with standard bodies in the same aged individual group, are occasionally appeared from fifth zoea larva stage, and occurence of it be tempted to relate with the factor of trophic condition. This paper was dealt with a comparative morphological view on big individuals, comparing with standard type in the same aged group, to solve the problem on (1). the existence or nonexistence of stages which it is easier to occurence of big individuals, (2). the rate of development in several appendages of an individual, and (3). a happening of skipping whether it certainly occur or not, during newly hatched zoea larvae develop to post larva stage. The results of the above are as follow: (1). the stages which is easier to occur of big individuals are fifth and seventh stage in this species, (2). even same individual, development of several appendages differs more or less on the rate of growth in accordance with its parts, (3). Evidence which skipping phenomenon is occured, during development through zoea larvae to post larvae, couldn't confirm.

• Korean journal of applied entomology
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• v.35 no.2
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• pp.146-152
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• 1996

The adult of the aphidophagous ladybeetle, Harmonia axyridis, was reared by feeding an artificial diet (chicken's 1iver:sugar = 5:2, v/w). The adult ladybeetle used was from pupae collected in the field in midJuly. Yolk protein production, fecundity and longevity of the adult female were checked under 3OoC, 16L:8D. Yolk proteins (Vg3 and Vg4) were detected from the hemolymph of the female 5 days after the emergence. One hundred pairs of female and male were reared separately. First oviposition started from 7 days afier the emergence and laid an average of 163.9k144.0 eggs during lifetime. Seventeen females did not lay eggs at all. Longevity of the female was 60.6k16.9 days. The application of a JH analog, methoprene (2.5 pg), caused the occurrence of yolk proteins in the hemolymph 1 day earlier than that of the control. Weekly application of 2.5 pg of methoprene induced 83.3% from the 30 females to lay eggs within 15 days after the emergence, while the control had 51% only. Methoprene made all females lay eggs, causing the increase in the fecundity. Though the longevity of the female was not effected by the weekly application of methoprene, one time excess dose (12.5 pg) caused high in mortality and low in fecundity.

• The Korean Journal of Malacology
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• v.26 no.2
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• pp.151-156
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• 2010

For industrialization of the hard clams, Meretrix petechiails (Lamarck), spawning was induced per spawning induction technique in the artificial maturation group administered of parent maturation control and the natural maturation group of which parents were transported for artificial spawning per time period. Then, fertilization rates, hatching rates and D-shaped larva development rates were investigated. In addition, growth and survival rates of larvae were investigated per larva breeding technique. The results of spawning induction by exposure in the artificial maturation group indicated that response rates were relatively higher at 23% and 32% respectively at the 4th hour and the 8th hour of exposure. In terms of water temperature increase, responses began only when the temperature reached $28^{\circ}C$ or higher. In the experiment group administered with both exposure and water temperature increase techniques, response rate was found to be 45% or higher at the 4th hour of exposure and the temperature of $28^{\circ}C$. At the temperatures of 29, 30 and $31^{\circ}C$, significant differences were not observed. Therefore, it was indicated that the response rates of parent hard clams were higher toward water temperature increase than exposure time. As for spawning induction per time period of the transported parent group, response rate and D-shaped larva development rate were the highest at 67.6% and 96% respectively on August 6, 2009. In terms of water temperatures during larva breeding experiment, growth was faster as water temperature was higher. In addition, growth and survival rates were relatively higher at the salinity of 25. In terms of stocking density, growth and survival rates were relatively higher at 5 inds./mL.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.54-55
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• 2003

해수관상용으로 그 인기도가 높은 상어류는 모두가 자연에서 채집된 후 관람용 대형수조 또는 개인용 소형수조 내에서 순치 후 관상용으로 사육되고 있다. 그러나, 자연에서 채집된 대부분의 개체는 인위적인 수조 시설 내에서의 생활에 적응하는데 실패하여 결국 거의 대부분의 개체가 짧은 기간 내에 사망하게 된다. 따라서 이 연구는 인공 종묘 생산 시설에서 해수 관상용 상어류의 종묘 확보를 목적으로 실시하였다. 우리나라 제주 연안에서 채집이 비교적 용이한 까치상어 Triakis scyllium와 별상어 Cynias manazo 2종의 상어 친어를 확보하여 인공 사육 시설에서 사육하면서 수조 내에서의 자연 교미와 자연 산란에 성공하였다. 친어와 치상어의 사육관리는 자연해수를 사용하여 15~40ton 크기의 콘크리트 수조에서 사육하면서 전갱이와 같은 생사료 또는 전갱이와 어분을 주성분으로 하는 습사료를 만들어 1일 1회 급이하였다. 까치상어의 인공 종묘는 수 차례의 사산을 거듭한 끝에 2003년 5월 18일과 6월 8일 두 차례에 걸쳐서 매우 활력이 좋은 상태의 치어가 출산하였다. 별상어도 수 차례의 사산을 거듭한 끝에 2003년 5월 25일 건강한 상태의 치어를 출산시키는데 성공하였다. 지금까지 산출된 까치상어의 출산시 평균 전장은 25.70$\pm$0.88cm 평균 체중은 58.32$\pm$4.86g이었으며, 별상어의 출산시 치어의 평균 전장은 27.26$\pm$0.57cm였고 평균 체중은 55.32$\pm$1.69g로 두 종 상어의 평균 전장과 평균 체중은 유사한 경향을 보였다. 그러나, 산출 후 사육 관리 과정에서 관찰된 까치상어와 별상어의 월간 성장 중 전장을 비교한 결과, 까치상어는 1개월 전 대비 15.06%의 성장을 보였으나, 별상어는 3.15%의 성장을 보여 까치상어 보다는 별상어의 길이 성장이 느린 것을 알 수 있었다. 까치상어와 별상어의 월간 성장 중 체중을 비교한 결과에서도 까치상어는 1개월 전 대비 34.11%의 성장을 보였으나, 별상어는 2.25%의 성장을 보여 까치상어 보다는 별상어의 체중 성장이 매우 느린 것을 알 수 있었다. 출산 관리중인 까치상어와 별상어 치어의 성비를 조사한 결과, 까치상어는 ♀:♂=1:1.28, 별상어는 ♀:♂=1:2.50으로 별상어의 암 수 성비가 매우 불균형한 것을 알 수 있었다. 그리고 까치상어와 별상어의 암ㆍ수간 성장 차이를 조사한 결과, 까치상어와 별상어의 암ㆍ수간 성장 (전장, 체장 및 체중)에는 큰 차이가 없었다. 현재, 인공 사육 시설에서 관리중인 까치상어는 64마리이며 별상어는 7마리이다. 결국 소형의 수족관내에서 해수 관상어로서 사육하기 위한 조건으로서 너무 급격한 성장을 하는 어종인 경우에는 관상 기간이 짧아져 조기 폐기하여야 하는 단점을 감안하면 까치상어 보다는 별상어의 관상가치가 높을 것으로 판단되며, 반대로 대형 관람용 수족관의 경우에서는 별상어 보다 성장이 빠르고 운동력이 활발한 까치상어를 전시관람용으로 이용할 경우 전시효과가 높을 것으로 판단된다.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.91-95
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• 2006

This paper reports a simple rearing system for Cnaphalocrocis medinalis based on corn seedlings diet. C. medinalis population under this system has been maintained by four stages (egg, young larva-1st to 3rd instar, old larva-4th to prepupa, 3nd adult) at $25{\pm}2^{\circ}C,\;40{\pm}10%$ RH, 3nd 16L:8D photoperiod in a laboratory. We have elaborated a new egg collection method using a polystyrene container $(top\;{\phi}11.3\;{\times}\;bottom\;{\phi}\;{\times}\;H8cm)$ which has made a great contribution in easy collection of eggs and storage of them for long period. Under this system, pupation and adult emergence rate, and pupal weight of C. medinalis population were close to the other reports by com seedling diet, and superior to those by artificial diets. Therefore, this rearing system would be useful in obtaining C. medinalsis population suitable to each specific needs.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.96-101
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• 2014

The present study was conducted for two years to develop the indoor breeding system of mole cricket, Gryllotalpa orientalis Burmeister. In 2013, the total mean number of progenies was $430.{\pm}20.0$ ($mean{\pm}SE$); $15.3{\pm}25.8$ of 2nd-3rd instars, $28.8{\pm}24.0$ 3rd-5th and $2.4{\pm}2.7$ 6th-8th. In 2014, the rearing yielded similar result to that of 2013; $43.5{\pm}31.0$ in total with $25.8{\pm}25.6$ of 2nd-3rd instars, $23.6{\pm}21.3$ 3rd-5th and $2.9{\pm}2.3$ 6th-8th. The successful breeding rate using indoor cages was 46.9% in 2013 and 50.0% in 2014. In total 147 individuals were successfully reared in an outdoor rearing cage with 25 females released and 78 individuals in an indoor rearing cage with 100 females and 50 males released. 86.4% and 89.7% of the nymphs reared were collected within 25cm depth of the soil in the outdoor and indoor cage, respectively.

• Korean journal of applied entomology
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• v.50 no.1
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• pp.55-63
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• 2011

This study was conducted to develop an artificial diet for the mugwort looper, Ascotis selenaria (Lepidoptera: Geometridae), which is an insect pest to leaves of citrus (Citrus unshiu). Corn and soybean powder were selected as main nutrient sources for larvae of A. selenaria after several diets consisted of wheat germ, corn, kidney bean and/or soybean were tested for larval development and survival. A higher amount of the main nutrients in the diet increased the larval survivorship. Addition of yeast and cholesterol in diet increased the larval survivorship. Finally the composition of diet was decided as followings; corn 100 g, soybean 100 g agar 25 g, Brewers' yeast 30 g, cholesterol 0.5 g, Vanderzant vitamin mixture 2 g, Wesson's salt mixture 2 g, sorbic acid 2 g, ascorbic acid 2 g, and methyl-4-hydroxybenzoate 2.5 g, and distilled water 1 liter. Development periods of larvae and pupae, survival rate and fecundity of A. selenaria reared on the diet were not significantly different with those on the host plant, citrus leaves. Larvae of early instars were reared in a group, while larvae of later instars (5-6th) were reared individually. Adult mating was conducted in a plastic cage and an oilpaper covered with a gauze was provided as an oviposition site.

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.27-32
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• 2012

The aim of this study is to develop indoor-rearing methods of the diving beetles. In nature, both the adult diving beetle and its larvae are voracious aquatic predators. The larvae beetles hunt relatively bigger size of tadpoles and small fish for food source. However, due to difficulties of the food supplement for rearing diving beetles at indoor-condition further motivated us to develop new artificial food. Three separate experiments were performed. In the first experiment, adult beetles were provided with one of the several food choice treatments to self-compose their preferred foods that are affordable on the market at lower price. The second experiment was also to develop artificial diet that is possible for rearing larvae beetle under indoor condition. The larvae beetles were restricted to raw squid, artificial food source and mosquito larvae as a control at the first stadium and small fish and raw squid during second to third stadium duration. According to our result, adult beetles selected a food that made of boiled squid and dead small fish while, the young larvae consumed small fish, mosquito larvae and raw squid. Although, the larval food restriction on law squid caused noticeable decrease in survival, the result still supported the possible survival rate of keeping larvae at indoor condition. Moreover, pupation rate experiments, in which groups of larvae were placed at different mats, natural soil and fermented sawdust, showed that 80% of diving beetles pupated on the sawdust. This result indicates that female beetle preferentially selected to oviposit along soft and moist area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.233-238
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• 1981

A recirculating water system without filter bed was used for a high density experimental rearing of common carp, Cyprinus carpio, during the summer of 1981. Of the water system, 3 tanks were used for stocking each tank with 10,000, 20,000 and 30,000 common carp fingerlings which averaged 0.6 g. The water in the system was maintained in dark green colour by heavy growth of phytoplankton during the most period of the experiment. In this experiment, the best daily growth rate, $3.7\%$, was obtained from the 10,000 fish group followed by $2.8\%\;and\;3.0\%$ from the 20,000 and 30,000 fish groups respectively. Thus there was no significant difference between the latter two groups. Especially, notably high concentration of total ammonia, $5\sim7$ ppm ammonia-N, in the culture water appeared during the most experimental period, but this did not seem to have affected feeding and growth of the fish when the water was kept at heavy bloom. It was also obvious that the outbreak of columnaris disease was effectively depressed in this green water recirculating system compared to that in clarified recirculating system. One tank ($7m^2$) of this system was stocked with 446 common carp of intermediate size averaging 352.2 g and after 40 day rearing they grew to 486.3 g in average with a daily growth rate of $0.8\%$. This result was a little inferior to that reared in a tank of the clarified water system as control which showed $1.0\%$ daily growth rate. Therefore, before an initiation of the commercial production of fish in this method, further studies concerning the amount of ammonium with its effect on the fish under rearing in this system and the columnaris disease problem should be carried out.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.183-191
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• 2023

After examining the temperature change in berry sawdust during the fermentation period and the developmental characteristics of Protaetia brevitarsis larvae according to the fermentation period, it was found that the fermentation speed was faster than with oak sawdust and a fermentation period of 50-70 days was the most suitable for larval growth. During the fermentation period, the temperature change in sawdust was quickly fermented as berry sawdust passed a high temperature of more than 60℃ in the early stages of fermentation compared to fermentation speed of oak sawdust, and stabilized within 60 days. In individual breeding, the total growth period of P. brevitarsis larvae by fermentation period was the longest at 104.8 days after fermentation for 30 days, which was a significant difference. There was no difference in the growth period between 50 and 90 days of fermentation. Oak sawdust tended to be the same as berry sawdust, but the larval development period was approximately 30 days. The developmental period by larval stage differed greatly between berries and oak sawdust. The weight of larvae according to the fermentation period of sawdust was the lowest after 90 days of fermentation, with a long fermentation period for both berry and oak sawdust, and there was no difference in the 30-70 days fermentation periods. The survival rate was more than 95% in individual breeding, and there was no difference among the fermentation periods. However, in group breeding, the survival rate was lower than that of individual breeding and was lowest at 90 days of fermentation. In group breeding, the ratio of larvae weighing ≥ 2.5 g, which is the standard for shipment, was 85% after 30-70 days of fermentation of berry sawdust, but was lower at 70% after 90 days of fermentation, which was a significant difference. The survival rate with oak sawdust showed the same trend, which was approximately 10% lower than that with sawdust. In addition, the larvae from the berry sawdust weighed > 2.5 g in 40-60 days, while it took 75-105 days with oak-fermented sawdust.