• Applied Biological Chemistry
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• v.18 no.3
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• pp.167-176
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• 1975

As a study on the ATP-inhibited ribonuclease of Bacillus subtilis the screening work for obtaining the ATP-inhibited ribonuclease negative mutant were carried out. And mutant strain was selected by the treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). For the selected strain the enzyme purification and some physiological properties were examined and the results obtained were as follows. 1. Among tested 1817 strains with the treatment of NTG, 101 strain was selected as a mutant strain. 2. ATP-inhibited ribonuclease was tentatively purified by several independent column chromatography. The results with Sephadex G-75 column were 30 times purification, 99% recovery, and 20 times purification, 98% recovery, respectively. 3. ATP-inhibited ribonuclease was purified by 60 times through acid treatment, ammonium fractionation, and two successive chromatography. 4. The purified ribonuclease were shown to be effectively concentrated in robonnclease content and to have reduced numbers of protein band on Disc electrophoresis. 5. This enzyme degraded single-stranded RNA to 2',3'-cyclic AMP, 2',3'-cyclic CMP, 2',3,-cyclic GMP, 2',3'-cyclic UMP and some unknown intermediates. The enzyme could not split double-stranded RNA.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.89-99
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• 2004

Stretch-activated channels (SACs) responds to membrane stress with changes in open probability (Po). They play essential roles in regulation of cell volume and differentiation, vascular tone, and in hormonal secretion. SACs highly present in Xenopus oocytes and Ascidian oocytes are suggested to be involved in the regulation of pH and fluid transport to balance the osmotic pressure, but remain unclear in mammanlian oocytes. This study was investigated to find the presence of SACs in hamster oocytes and to examine their electrophysiological properties. To infer a role of SAC in relation to the development of early stage, we followed up to the stage of two-cell zygote with patch clamp techniques. Single channels were elicited by negative pressure (lower than ­15 cm$H_2O$). Interestingly, SACs were dependent on permeable cations such as $Na^+$ or $K^+$. As permeable cation removed from both sides across the membrane, SAC activity completely disappeared. When permeable cations present only in intracellular compartment, outward currents appeared at positive potentials. In contrast to this, inward currents occurred only at the negative voltage when permeable cation absent in cell interior. These result suggests that SAC carry cations through the nonselective cation channel (NSC channel). Taken together, we found that stretch activated channels present in hamster oocyte and the channel may carry cations through NSC channels. This stretch activated-NSC channels may play physiological role(s) in oocyte growth, maturation, fertilization and embryogenesis in fertilized oocytes to two-cell zygotes of hamster.

• Tunnel and Underground Space
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• v.24 no.6
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• pp.440-448
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• 2014

Hydraulic fracturing technique has been applied in various fields in order to improve the recovery of energy resources such as gas, oil and geothermal energy and research about finding out hydraulic fracturing mechanism and application has been steadily proceeded. In this study, for effective hydraulic fracturing, a scale modeling was progressed to simulate similarly with the actual site. In order to analyze the development aspect of surface crack initiation pressure during hydraulic fracturing followed by different conditions, the number of guide holes hydraulic fracturing test was carried out by setting up a hydraulic fracturing test equipment. Also, through the result, we tried to derive reliable results by comparing and analyzing the value of numerical modeling which is obtained based on the physical properties and mechanical properties with 3DEC, a three-dimensional discrete element method program. As a result, it is considered possible to generate effective crack using the guide hole.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.6
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• pp.1277-1285
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• 2015

It has been known that the conventional retaining wall system with timber lagging and H pile has several problems such as the irregular gap between H-piles, cutting or adding to standard timber, back fill over first step excavation, and especially break-down accident at the disjoint of wall system. In the practical excavation, these problems may lead to worker's accident and the inefficiency of construction economy. To solve the above problems, a new method using prefabricated retaining wall was proposed and verified. The characteristics of the new method is to replace timber wall as free-sliding steel-lagging and connector. To check its verification and application, laboratory tests such as bending strength, tensile strength, and fatigue strength were carried out. Also, a pilot test in the field and numerical simulations under various ground conditions were performed. From the researches, it is found that the prefabricated retaining wall plate can be superior to the conventional timber lagging plate in the strength. It is also found that the proposed methods can be effective in the reuse of retaining wall plate and safe in the disjoint of wall system. Finally, it is desired that the proposed method will be effective in the reduction of the imported timbers and helpful in the safety of retaining wall construction.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.304-307
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• 2005

Multiresidue method was set up for the simultaneous determination of various residual pesticides in potatoes and carrots, which was analyzed by gas chromatography-electron capture detector/nitrogen phosphorus detector. Method consisted with acetone/acetonitrile (9 : 1) extraction and dichloromethane partition, followed by florisil cartridge purification with hexane/dichloromethane/acetonitrile (50 : 45 ; 5) elution. Among 197 pesticides (194 kinds) spiked to food materials, 143 and 155 pesticides were recovered over 70% on potatoes and carrots, respectively. Nineteen pesticides including bromacil, cyproconazole, were not detected in water and sample matrices. Matrix components may affect the low detections of 25 pesticides such as benfuracarb, bitertanol from potatoes and 16 pesticides such as bitertanol, carbosulfan from Carrots. Some pesticides including dichlobenil, fluoroimide and iprodione were highly detected from one or both matrices even though they were not detected from water.

• International Commerce and Information Review
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• v.16 no.4
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• pp.171-190
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• 2014

A short consumption cycle caused by the technological development and the diversification of customer lead to both the dynamic growth of the industry and the waste recycling issue at the same time. Including Korea, the situation is particularly worrisome in some countries, such as India and China, where acute environmental hazards have resulted from a combination of a lack of recycling centers' capacity and the domination of a large backyard recycling sector. A study about to maximize the current recycling center efficiency with minimal changes is required. In this study, we suggest the optimal location selection method for the recycling center based on the well-known reverse logistics cost minimization model. An actual recycling data about a specific electronic equipment and region in Korea are used for the verification of the method suggested.

• Korean Journal of Social Welfare
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• v.65 no.3
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• pp.131-153
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• 2013

This study examine the selection criteria for recruiting social workers, commonly agreed by the persons in charge of human resources. We visited 46 social welfare facilities and performed interviews individually with them. Thirty three items belong to 7 different areas (ability, career, suitability and agreement to the facility, characteristics, recommendation, religion) were made based on the results of interviews, and these items were sent to social welfare facilities in 16 different cities in Korea for asking questions (from Oct, 2012 to Apr, 2013). One hundred and sixty cases were collected and among them 45 cases exhibiting consistency rate less than 0.2 were finally selected. Superior scope and subordinate scope were extracted and comparisons between day care centers and living institutions were made. Results demonstrated that ability of social workers was the most important for superior scope and suitability and agreement of social workers to both day care centers and living institutions were the most important factor for recruiting workers.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.339-346
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• 2018

Cyanazine is a member of the triazine family of herbicides. Cyanazine is used as a pre- and post-emergence herbicide for the control of annual grasses and broadleaf weeds. This experiment was conducted to establish a determination method for cyanazine, as domestic unregistered pesticide, residue in major agricultural commodities using HPLC-DAD/MS. Cyanazine was extracted with acetone from representative samples of five raw products which comprised apple, green pepper, Kimchi cabbage, hulled rice and soybean. The extract was diluted with saline water and partitioned to dichloromethane for remove polar extractive in the aqueous phase. For the hulled rice and soybean samples, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized florisil column chromatography. On a $C_{18}$ column in HPLC, cyanazine was successfully separated from co-extractives of sample, and sensitively quantitated by diode array detection at 220 nm. Accuracy and precision of the proposed method was validated by the recovery experiment on every major agricultural commodity samples fortified with cyanazine at 3 concentration levels per agricultural commodity in each triplication. Mean recoveries were ranged from 83.6 to 93.3% in five major representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation(LOQ) of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring(SIM) technique was also provided to clearly identify the suspected residue.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.3
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• pp.117-124
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• 2007

This paper is about the Extra-High voltage distribution system in the customer's area. The power loss in the distribution system in the customer's area is disregarded and rarely managed so far. But, economically, this loss is not small quality to ignore. So, in this paper, we calculate the power loss of the Extra-High voltage distribution system in the customer's area by changing the locations of power transformer and other power facilities to decrease power loss in decreased secondary line length. And we also show the payback time of the proposed Extra-High voltage distribution system in the customer's area by simplified calculations.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.3
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• pp.60-67
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• 2016

In cumulative transfer functions, as number of transfer increase, the impact of individual transfer to transfer cost increase linearly or non linearly. This function can effectively explain various passengers's travel behavior who choose their travel routes in integrated transit line networks including bus and railway modes. Using the function, it is possible to simulate general situations such that even though more travel times are expected, less number of transfer routes are preferred. However, because travel cost with cumulative transfer function is known as non additive cost function types in route search algorithms, finding an optimal route in integrated transit networks is confronted by the insolvable enumeration of all routes in many cases. This research proposes a methodology for finding an optimal path considering cumulative transfer function. For this purpose, the reversal phenomenon of optimal path generated in route search process is explained. Also a heuristic methodology for selecting an optimal route among multiple routes predefined by the K path algorithm. The incoming link based entire path deletion method is adopted for finding K ranking path thanks to the merit of security of route optimality condition. Through case studies the proposed methodology is discussed in terms of the applicability of real situations.