• 한국식품과학회지
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• 제32권4호
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• pp.949-958
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• 2000

국내 식물 150종과 인도네시아 식물 82종의 항균활성을 검색하였다. 각 시료의 70% ethanol 추출물을 사용하여, 세균류인 Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa 및 효모인 Candida albicans, 사상균인 Aspergillus niger를 대상으로 측정하였다. 검색 결과, 국내산 17종과 인도네시아산 18종이 비교적 항균활성이 좋았으며, 이중 강황, 소목, 육두구, 익지인, 죽엽, 초두구 등과 asam, bolong-bolong, clove, gambir, klembak, pasak bumi 등이 우수한 항균력을 나타내었다. 가장 항균력이 우수한 육두구(Myristica fragrans)와 bolong-bolong(Melaleuca leucadendra)의 주 활성 분획을 분석한 결과, 육두구의 주 항균성분은 limonene과 caprylic acid로, Bolong-bolong의 주 항균성분은 ${\alpha}-terpineol$로 나타났다.

• 생약학회지
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• 제46권3호
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• pp.229-233
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• 2015

The anti-nociceptive effect of an ethanol extract and its various solvent fractions from Curcuma longa Linne ethanol extract was studied using the writhing test in mice. Different fractions by various solvent extraction from Curcuma longa Linne ethanol extract were administered orally 1 hr or time-course (0.5, 1, 2 and 5 hr) before intraperitoneal injection of acetic acid. After treatment with 30% ethanol extract and n-butanol fraction, CB-1, at a dose of 250 mg/kg, the significant writhing responses were 87.5 ± 13.4 (inhibition rate 31%, p<0.01) and 75.1 ± 11.1 (inhibition rate 41%, p<0.01) lower than the control group. At the dose of CB-1 50 mg/kg and 250 mg/kg, CB-1 showed a similar activity comparing to diclofenac of 10 mg/kg. A time-course experiment was performed, which involved oral administration of CB-1 (250 mg/kg) at 0, 0.5, 1, 2, and 5 hr before acetic acid intraperitoneal injection. The most effective time of CB-1 was 30 min before treatment and persisting until 2 hr. This study showed that Curcuma longa Linne has anti-nociceptive properties comparable with those of diclofenac, which suggests promise for the treatment of intractable visceral pain in humans. Major components of the active fraction are identified as curcumin, cyclocurcumin and demethoxycurcumin.

• 한국응용과학기술학회지
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• 제28권4호
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• pp.393-401
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• 2011

Natural Curcumin belongs to Zingiber Officinale Roscoe was known to possess natural odor, natural taste, natural color, and other pharmaceutical & chemical characteristics. Natural Curcumin extract was made to use ethanol as a solvent was to show a yellow color having state of solid powder and an active component. Natural Curcumin extract tested pharmaceutical & chemical experiment to dilute in curcumin 1%-water solution. Curcumin extract tested antimicrobial experiment using microbe, and tested dye experiment using fiber. Some conclusions in the result of characteristics experiment was obtained as follow. The result of antimicrobial experiment showed that the growth of staphylococcus aureus (ATCC-001) and aspergillus niger (ATCC-002) as microbes decreased according to passage of time. This phenomenon could know that Curcumin compoment showed influence to antimicrobial effect. Also, the result of dye experiment showed that cotton and sick with fiber dyeing dyed in direction of dark yellow color. This phenomenon could know that Curcumin extract showed influence to dyeing effect in observation of optical electron microscope(OEM.) The result of instrument analysis ascertained inorganic components of K(53.300ppm), Na(1.150ppm), Ca(0.711ppm), Ti(0.351ppm), Li(0.256ppm), Cu(0.233ppm) etcs from Curcumin component with ICP/OES, and ascertained organic components of propanoic acid(1.859), benzene(10.814), phenol(14.194) etcs from Curcumin component with GC/MSD.

• 한국약용작물학회지
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• 제22권5호
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• pp.378-383
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• 2014

Curcuma longa L. leaf was extracted by water at $60^{\circ}C$ for 12 hours after being treatment of Ultra High Pressure under 500 MPa for 5-15 minute. The high pressure extraction for 15 minute (HPE15) was measured the highest extraction yield of 25.48% (w/w), compared to those from conventional extraction methods. The HPE15 showed the lowest cytotoxicity as 11.97% in adding $1.0mg/m{\ell}$ of concentration. Also, HPE15 was measured the highest inhibition of hyaluronidase as 44.48% in adding $1.0mg/m{\ell}$. In addition, The production of NO from macrophages was measured as $7.06{\mu}M$ in adding $1.0mg/m{\ell}$ of HPE15, which was lower than the those from others processes. Finally, HPE15 significantly reduced up to $649.44pg/m{\ell}$ of $ProstaglandinE_2$ production from UV-irradiation. These results suggest that the Curcuma longa Linne leaf extract from high pressure process might enhance the skin immune activities possibly by high elution of active components than other processes.

• 한국약용작물학회지
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• 제22권2호
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• pp.121-126
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• 2014

This study was designed to evaluate antioxidant activity of Curcuma longa L. leaves treated by ultra high pressure extraction. Curcuma longa L. leaves was subjected to 5,000 bar for 5 and 15 min at $25^{\circ}C$ The highest phenolics and flavonoids content was observed in the treatment of high pressure extraction for 15 min ($308.28{\mu}g/mg$, $124.33{\mu}g/mg$). The DPPH scavenging activity was 82.34% at $1.0mg/m{\ell}$ of Curcuma longa L. leaves treated by ultra high pressure process for 15 min. The highest SOD-like acitivity of Curcuma longa L. leaves ($1.0mg/m{\ell}$) was observed at ultra high pressure extraction for 15 min (67.54%). The high pressure extraction significantly increased the contents of phenolics and flavonoids and also enhanced the antioxidant activity. These results provide useful information for enhancing biological properties of Curcuma longa L. leaves.

• 대한본초학회지
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• 제21권1호
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• pp.71-77
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• 2006

Objectives : This investigation was undertaken to evaluate the anti-proliferation of CURCUMAE LONGAE Rhizoma extracts using MCF-7, human breast cancer cells. Methods : MCF-7 cells were cultured in Dulbecco's modified Eagle's medium/F12 supplemented with 10% fetal bovine serum and antibiotics. At varying times after extract treatment, cells were harvested with scraper and processed for analysis of proliferation, cytotoxicity. Results : The extract of CURCUMAE LONGAE Rhizoma strongly inhibits the proliferation of MCF-7 cells in a dose and time-dependent manner. Sulforhodamine B assay showed that the addition of ethanol extract of CURCUlVIAE LONGAE Rhizoma reduced the viability of MCF-7 cells in a dose-dependent manner. Conclusion : So, it can be concluded that CURCUMAE LONGAE Rhizoma have an inhibitive effect on MCF-7 human breast cancer cells.

• 한국약용작물학회지
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• 제8권2호
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• pp.109-116
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• 2000

115 종의 생약재 중에서 식품부패성 및 병원성 미생물을 대상으로 항균활성을 나타내는 생약자원을 검색한 결과 식품부패성 세균 Bacillus subtilis에 강한 항균활성을 보인 것은 단삼, 관중, 가자, 독활, 파고지, 오미자이었으며, 식중독균인 효모 Candida albicans IFO6258에 대해서는 지모와 관중 두 생약재가, 식품부패성 효모 Schizosaccharomyces sp.에 대해서는 지모, 관중, 원지가 각각 강한 항균 활성을 보였다. Streptococcus mutans에 대해서는 관중, 단삼, 소목, 방기, 오미자, 오배자, 고삼, 목향이 강한 항균 활성을 보였다. 피부 각층 및 모발에 침입하여 피부병을 야기하는 백선균 Trichonphyton mentagrophytes에 대해서는 지모, 강황, 목향, 호장근, 지유, 황금, 가자들이 항균활성을 나타내었다 . 항균spectrum이 넓으면서 비교적 항균활성이 강한 관중, 단삼, 가자, 황금에 항균활성이 강한 생약자원인 가자, 계피, 방기, 황금등의 생약재를 각각 1 : 1로 혼합한 추출물에 대한 항균활성을 조사한 결과 모든 혼합 추출물에서 단독 추출물 보다 강한 항균활성을 나타내었다.

• 대한한의학방제학회지
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• 제22권1호
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• pp.177-192
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• 2014

Objective : In order to investigate the effect of Curcumae Longae Rhizoma(CLR) extract on the hepatocellular carcinogenesis and acute liver damage induced by diethylnitrosamine(DENA) and $CCl_4$ in rats. Methods : Experimental groups were subdivided into four; normal group (Nor), acute liver damage and hepatocellular cancer inducing control group (Con), and CLR extract 200mg/kg/day (CAA) or 400mg/kg/day (CAB) administered groups to Con. Thereafter the changes of the body weight, the liver weight and the weight of liver/100g body weight, total cholesterol, HDL cholesterol, triglyceride, the activities of AST, ALT, ALP, LDH, AFP, SOD, catalase were measured. And we observed by optical and electron microscopy. Result : 1. The body weight was decreased in Con compared with Nor for 5 weeks, but increased in Con compared with Nor from 6 week to 9 week. During experimental period of total 9 weeks, CAA and CAB were increased compared with Con. 2. The liver weight was increased significantly (p<0.05) in Con compared with Nor. The weight of liver/100g body weight was increased significantly (p<0.05) in Con compared with Nor and decreased significantly (p<0.05) in CAB compared with Con. 3. The level of total cholesterol was increased in Con and CAA compared with Nor, but there was not statistically significant. The level of triglyceride was decreased in Con compared with Nor. But increased in CAA and CAB compared with Con. The level of HDL-cholesterol was significantly increased (p<0.05) in CAA and CAB compared with Con. 4. The activities of AST, ALT were increased in Con compared with Nor, but decreased in CAA compared with Con, significantly decreased (p<0.05) in CAB compared with Con. 5. The activities of ALP, LDH were increased in Con compared with Nor, but decreased in CAA and CAB compared with Con. 6. The activities of AFP was increased significantly (p<0.05) in Con compared with Nor, but decreased significantly (p<0.05) in CAA and CAB compared with Con. 7. The activities of SOD were increased in Con, CAA and CAB compared with Nor, but decreased in CAA and CAB compared with Con. The activities of Catalase was more increased in CAA and CAB compared than Con. 8. The results of light microscopical observation, a number of hepatocytes were damaged in Con compared with Nor and CAB. 9. According to the electron microscopical observation, irregular nuclear membrane, condensed nucleoplasm was observed in Con, the experimental group was observed in the nucleus of the well-preserved and evenly developed nucleoplasm. Conclusions : These results suggest that administration of CLR extract suppress or retard on the hepatocellular carcinogenesis and acute liver damage induced by DENA and $CCl_4$ in rats.

• 대한화장품학회지
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• 제44권2호
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• pp.125-131
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• 2018

아스코빅애씨드(비타민 C)는 스킨 케어 조성물에 널리 사용되어 왔다. 아스코빅애씨드는 항산화, 콜라겐 생합성 촉진, 피부미백효과 등 특별한 효과를 나타내며, 안티-에이징 활성성분으로 사용되고 있다. 하지만, 화장료 조성물에서는 아스코빅애씨드의 산화, 갈변, 변취와 같은 불안정한 문제가 있어 높은 함량을 적용하기에는 문제가 많다. 우리는 폴리올을 사용하여 아스코빅애씨드를 녹인 무수제형 조성물로 안정화를 진행했다. 무수제형은 아스코빅애씨드의 산화를 효과적으로 억제할 수 있는 조성물이다. 하지만, $5^{\circ}C$ 이하의 온도 조건일 때 무수제형 내에서 결정화가 일어나는 문제점이 있다. 우리는 수계에 아스코빅애씨드를 안정화하여 결정화 문제를 해결하고자 하였다. 본 연구에서는, 징크설페이트, 글루타치온, 강황뿌리추출물과 같은 항산화제를 사용하여 아스코빅애씨드를 안정화할 수 있는 최적의 비율을 찾고자 하였다. 조성물은 - $16^{\circ}C$, $5^{\circ}C$, $25^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$ and cycle($5-40^{\circ}C$) 인큐베이터를 사용하여 8주에 걸쳐 안정도를 확인하였다. 안정성 분석은 색상, 향, 상 분리, 침강 정도를 검토하였다. 아스코빅애씨드의 안정성은 HPLC 분석을 통하여 확인하였다.

• 대한본초학회지
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• 제20권4호
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• pp.27-31
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• 2005

Objectives : The anticancer response of three different types of water extracts of Zingiberaceae Curcuma longa L. tested for sarcoma 180. Only few studies carried out to investigate the effects of other contents of Curcuma longa L. in anticancer activities, therefore, in this study we have investigated the effects of other component then curcumin in Curcuma longa L. for anticancer a activities. Methods : Three different types of water extracts of Curcuma longa L. were prepared as follows. The sarcoma cells (S180) were maintained in Dulbecco's modified Eagle's medium (DMEM) and were seeded on 24-well cell culture cluster flat bottom with lid tissue culture treated non-pyrogenic polystyrene. The growth of sarcoma 180 was monitored for 1, 2 and 5 days. The sarcoma cells were pictured using inverted microscope and cell density was counted using hemocytometry. Results : After 5 days in the culture medium the results showed high growth of sarcoma 180 for control condition and the surface of CCP plates were fully covered with the cells. In case of medium in which the 10% of filtered water extract of Curcuma longa L. was added a very limited growth of sarcoma 180 was observed. The results were showed only small difference in cell density for two different concentrations of unfiltered water extracts of Curcuma longa L. whereasin case of filtered water extracts the control of sarcoma growth shows better result. Conclusion : The filtered water extracts showed the best result relatively to the unfiltered water extracts for two different concentrations. This indicates that the water extracts of Curcuma longa L. can have anticancer activities possibly without curcumin.