• Title/Summary/Keyword: 강합성

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Conformation and Biological Activity of the Neuropeptide $\gamma$ (Neuropeptide $\gamma$의 구조 및 생리활성)

  • Ku Hee-Jung;SEO Jung-Kil;KIM Eun-Hi;HUH Min-Do;CHUNG Joon-Ki;PARK Jang-Su;KANG Shin-Won;PARK Nam Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.2
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    • pp.244-251
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    • 1997
  • The relationship between structure and biological activity was studied on the three neuropeptides (mammalian, trout- and goldfish-neuropeptide $\gamma$) that were syntheized by the solid-phase method. Circular dichroism spectra showed that mammalian, trout- and goldfish-neuropeptide $\gamma$ adopted an unordered structure in buffer solution. In the-presence of neutral and acidic liposomes, mammalian and trout-neuropeptioe $\gamma$ also took a random structure. However, goldfish-neuropeptide $\gamma$ took an $\alpha-helical$ structure in acidic liposomes. The intestinal motility response was investigated with carp intestines, guinea-pig ileums and rat duodenums. In case of carp intestine, contractile activity was as follows : goldfish-neuropeptide $\gamma\simeq$ trout-neuropeptide $\gamma>$ mammalian-neuropeptide $\gamma$, On the other hand, the contractile activity of mammalian-neuropeptide $\gamma$ was more potent than trout- and goldfish-neuropeptide $\gamma$ in the guinea-pig ileums and rat duodenums. These results suggest that neuropeptide $\gamma$ show the species-specific activity.

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Cloning and Expression of Indole Oxygenase Gene Derived from Rhodococcus sp. RHA1 (Rhodococcus sp. RHA1 유래의 Indole Oxygenase의 클로닝 및 발현)

  • Kang, Mi-Suk;Lee, Jin-Ho
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.197-203
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    • 2009
  • An indole oxygenase originated from Rhodococcus sp. RHA1 was cloned into the expression vector, pTrc99A, in Escherichia coli, and designated pTCAN1. The pTCAN2 was constructed from pTCAN1 by the deletion of $lacI^q$ for the constitutive expression of indole oxygenase without adding IPTG in the medium. The complete open reading frame of indole oxygenase was 1,224 bp long, which encodes a protein of 407amino acids. Crude extracts of E. coli $DH5{\alpha}$/pTCAN1 and pTCAN2, respectively, were prepared and subjected to SDS-PAGE analysis. A band corresponding to molecular mass of about 43 kDa was appeared and this result correlated with the predicted molecular mass of cloned indole oxygenase. The E. coli harboring pTCAN1 and pTCAN2, respectively, showed blue color colony in LB plate. The pigment showing blue color was prepared from E. coli $DH5{\alpha}$/pTCAN2, and identified as indigo by experiments using spectrophotometer, HPLC, and TLC. The indigo-forming activity of indole oxygenases from the whole cell of E. coli $DH5{\alpha}/pTCAN1$ cultured at LB medium added 1mM of IPTG and that of E. coli/pTCAN2 showed about 1.75nmol/min/mg DCW (dry cell weight) and 3.85 nmol/min/mg DCW, respectively. Also, the E. coli $DH5{\alpha}$/pTCAN2 produced about $236{\mu}M$ of indigo after 48 hours incubation in TB medium supplemented with 2.5 mM of tryptophan.

Evaluation of Optimum Mix Proportion and Filling Performance of High-fluidity Concrete for SCP Module charging (SCP 모듈 충전용 고유동 콘크리트의 최적배합 도출 및 채움성능 평가)

  • Park, Gi-Joon;Kim, Sung-Wook;Park, Jung-Jun;Lee, Dong-Gyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.452-459
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    • 2017
  • In recent years, to reduce self-weight of structural elements, the use of SCP (Steel Concrete Plate) instead of prestressed concrete is increasing. Because SCP has a complicated sectional shape and includes a large number of studs, the use of high-fluidity concrete is required. Therefore, in this study, to prevent the restrained shrinkage behavior by the stud, the effects of using an expansive agent (EA) and shrinkage reducing agent (SRA) were investigated, and the optimal mixture proportions to maximize the filling capacity were determined based on the fine aggregate ratio. The test results indicated that the combined use of EA and SRA exhibited the smallest shrinkage. The ratio of the crushed sand and washed sea sand was determined to be 5:5, and the proper fine aggregate ratio was found to be 55.6%, because when the ratio was too high, a decrease in strength and an increase in shrinkage strain were expected. The high-fluidity concrete effectively filled the large-sized SCP module.

Effect of Forming Process and Particle Size on Properties of Porous Silicon Carbide Ceramic Candle Filters (성형공정(成形工程)과 원료입도(原料粒度)가 다공성(多孔性) 탄화규소(炭火硅素) 세라믹 캔들 필터 특성(特性)에 미치는 영향(影響))

  • Han, In-Sub;Seo, Doo-Won;Hong, Ki-Seog;Woo, Sang-Kuk
    • Resources Recycling
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    • v.19 no.5
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    • pp.31-43
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    • 2010
  • To fabricate porous SiC candle filter for filtration facility of the IGCC system, the candle type filter preforms were fabricated by ramming and vacuum extrusion process. A commercially available ${\alpha}$-SiC powders with various particle size were used as starting raw materials, and $44\;{\mu}m$ mullite, $CaCO_3$ powder were used as non-clay based inorganic sintering additive. The candle typed preforms by ramming process and vacuum extrusion were sintered at $1400^{\circ}C$ for 2h in air atmosphere. The effect of forming method and particle size of filter matrix on porosity, density, strength (flexural and compressive strength) and microstructure of the sintered porous SiC candle tilters were investigated. The sintered porous SiC filters which were fabricated by ramming process have more higher density and strength than extruded filter in same particle size of the matrix, and its maximum density and 3-point bending strength were $2.00\;g/cm^3$ and 45 MPa, respectively. Also, corrosion test of the sintered candle filter specimens by different forming method was performed at $600^{\circ}C$ for 2400h using IGCC syngas atmosphere for estimation of long-term reliability of the candle filter matrix.

Application of RAIDOM for Rainfall-Runoff Simulation (레이더영상 디지털변환(RAIDOM)의 강우-유출모의 적용성 연구)

  • Oh, Kyoung-Doo;Lee, Soon-Cheol;Ahn, Won-Sik;Choi, Byong-Gyu;Kang, Tae-Ho
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.684-688
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    • 2008
  • 레이더 강우와 관련한 대부분의 연구나 실무적용이 제한을 받는 이유는 레이더 반사도 등의 원시자료를 획득하기가 어려울 뿐만 아니라 이를 처리하여 수문해석에 적용하는 과정이 간단하지 않기 때문이다. 이를 해결하기 위하여 다음과 같은 내용을 연구하였다. (1) 레이더 영상자료를 실용적으로 활용하기 위한 '레이더 영상 디지털 변환법(RAIDOM)'을 연구 개발하였다. 또한 오프라인상에서도 기상청 레이더 합성 CAPPI 이미지 자료를 디지털 강우자료로 직접 변환할 수 있는 방법을 제시하였다. 이러한 기술은 앞으로 레이더 강우 연구와 레이더 강우의 활용성을 넓히는데 크게 기여할 것으로 기대된다. (2) RAIDOM 레이더 강우와 연계한 분포형 강우유출모형을 구축하였다. 본 연구에서는 DEM, 토지피복도, 토양도로부터 분포형 강우-유출모형의 매개변수를 산정하는 방법을 상세히 연구하여 제시하였다. 이러한 연구결과는 앞으로 분포형 강우유출모형에 대한 연구와 활용성을 넓히는데 기여할 것으로 기대된다. (3) 주요 관측 레이더 호우사상을 이용하여 RAIDOM 강우와 구축된 분포형 모형의 적용성을 검증하였다. 이를 위하여 먼저 강우유출자료가 체계적으로 관리되고 있는 평창강 국제수문개발계획(IHP) 시범유역의 자료를 이용하여 모형의 매개변수 보정을 수행하였다. 강우 전 하천의 기저유량과 유역의 초기함수조건을 제외한 나머지 매개변수는 유역특성을 나타내는 인자들이므로 모든 강우사상에 대하여 일정한 것으로 가정하여 매개변수 보정을 수행하였다. 6개 주요 호우사상에 대하여 보정한 결과 4개의 호우사상에 대하여 강우-유출과정을 거의 완벽하게 재현하였으며, 2개의 호우사상에 대해서는 수문곡선의 상승과 하강은 비교적 일치하나 첨두부에서 다소차이가 발생하였다. (4) 보정된 분포형 모형을 2006년 7월에 발생한 국지성 집중호우와 한강유역 중상류지역에 걸쳐 큰 홍수량을 발생시킨 2개의 호우사상에 대하여 레이더 강우자료를 적용하여 검토하였다. 검토결과 임진강유역 3개 수위관측소와 우이천수위관측소 및 중랑교수위관측소에서 모의된 홍수수문곡선은 실측치와 잘 일치하는 것으로 나타나 본 연구에서 제시한 RAIDOM과 이를 적용한 분포형 모형이 강우유출 모의를 위하여 활용될 수 있음을 보여주었다. 앞으로 태풍에 수반된 강우와 장마전선 등을 포함한 다양한 유형의 여러 가지 강우에 대한 적용을 통하여 모형의 검증과 보완을 수행하여 RAIDOM 레이더 강우와 분포형 강우유출모형을 연계한 홍수 예보 시스템으로 발전시켜 나갈 예정이다.

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Analysis of Flood Inundation using GIS (GIS를 이용한 홍수범람 분석)

  • Shim, Soon-Bo;Kim, Joo-Hun;Lim, Gwang-Seop;Oh, Deuk-Keun
    • Journal of the Korean Association of Geographic Information Studies
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    • v.6 no.1
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    • pp.132-142
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    • 2003
  • A significant deficiency of most computer models used for stream floodplain analysis, is that the locations of structures impacted by flood waters, such as roads, buildings, and bridges, cannot be effectively compared to the floodplain location. The purpose of this study is the integration of the HEC River Analysis System(HEC-RAS) with ArcView geographic information system to develop a regional model for floodplain determination and representation. Also this study presents to enable two- and three-dimensional floodplain mapping and analysis in the ArcView. The methodology is applied to a Yeoju of Kyunggi-do, located in South Han River Basin. A digital terrain model is synthesized from HEC-RAS cross-sectional data and a digital elevation model of the study area. The flood plain data developed in ArcView was imported into HEC-RAS where it was combined with the field surveyed channel data in order to construct full floodplain cross sections that reflected accurate channel and overbank data for the HEC-RAS model. The flood plain limits could be expressed more accurately on ArcView by using water level data to be computed in HEC-RAS program. The computed water surface elevations and information of cross-section must be manually plotted in order to delineate floodplains. The resulting of this study provided a good representation of the general landscape and contained additional detail within the stream channel. Overall, the results of the study indicate that GIS combined with HEC-RAS is proven to be very useful and efficient for the automatic generation of flood maps, and an effective environment for floodplain mapping and analysis.

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3-D Radiosurgery Planning Using Personal Computer (Personal Computer를 이용한 3차원적 뇌정위적 방사선 치료계획)

  • 서태석;서덕영;박찬일;하성환;강위생
    • Progress in Medical Physics
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    • v.3 no.1
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    • pp.63-69
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    • 1992
  • Recently, stereotactic radiosurgery plan is required with the information of 3-D image and dose distribution. The purpose of this research is to develop 3-D radiosurgery planning system using personal computer. The procedure of this research is based on three steps. The first step is to input the image information of the patient obtained from CT or MR scan into personal computer through on-line or digitizer. The position and shape of target are also transferred into computer using Angio or CT localization. The second step is to compute dose distribution on image plane, which is transformed into stereotactic frame coordinate. and to optimize dose distribution through the selection of optimal treatment parameters. The third step is to display both isodose distribution and patient image simultaneously using superimpose technique. This prototype of radiosurgery planning system was applied recently for several clinical cases. It was shown that our planning system is fast, accurate and efficient while making it possible to handle various kinds of image modelities such as angio, CT and MRI. It is also possible to develop 3-D planning system in radiation therapy using beam's eye view or CT simulation in future.

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Supported Metal Nanoparticles: Their Catalytic Applications to Selective Alcohol Oxidation (금속 나노 촉매를 활용한 선택적 알코올 산화 반응)

  • Hussain, Muhammad Asif;Joseph, Nyanzi;Kang, Onyu;Cho, Young-Hun;Um, Byung-Hun;Kim, Jung Won
    • Applied Chemistry for Engineering
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    • v.27 no.3
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    • pp.227-238
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    • 2016
  • This review article highlights different types of nano-sized catalysts for the selective alcohol oxidation to form aldehydes (or ketones) with supported or immobilized metal nanoparticles. Metal nanoparticle catalysts are obtained through dispersing metal nanoparticles over a solid support with a large surface area. The nanocatalysts have wide technological applications to industrial and academic fields such as organic synthesis, fuel cells, biodiesel production, oil cracking, energy conversion and storage, medicine, water treatment, solid rocket propellants, chemicals and dyes. One of main reactions for the nanocatalyst is an aerobic oxidation of alcohols to produce important intermediates for various applications. The oxidation of alcohols by supported nanocatalysts including gold, palladium, ruthenium, and vanadium is very economical, green and environmentally benign reaction leading to decrease byproducts and reduce the cost of reagents as opposed to stoichiometric reactions. In addition, the room temperature alcohol oxidation using nanocatalysts is introduced.

Biological Control of Perilla Sclerotinia Rot Caused by Sclerotinia sclerotiorum Using Bacillus megaterium N4. (Bacillus megaterium N4에 의한 들깨 균핵병 (Sclerotinia sclerotiorum)의 생물학적 방제)

  • 문병주;김현주;송주희;이광열;백정우;정순재
    • Journal of Life Science
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    • v.14 no.5
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    • pp.761-769
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    • 2004
  • This study was investigated the occurrence of sclerotinia rot caused by Sclerotinia sclerotiorum at the major perilla cultivating area, Gangdong-dong, Gangseo-gu, Busan in 1998. The incidence of this disease ranged from 8.1 to 28.3% at Gangdong-dong area during the growing seasons. Symptoms of the disease initially appeared damping-off of infected stems and soft-rot on the leaves of perilla. Under the relatively high humidity, abundant white mycelia of the pathogen formed on the lesion developed into black sclerotia later and the infected leaves were finally fell down. Sixteen isolates, Sl-S16, isolated from diseased lesions showing typical symptoms, and pathogenicity was tested using mycerlial disks. Among them, S2 isolate showing the most strong pathogenicity was selected and identified as Sclerotinia sclerotiorum on the basis of morphological and cultural characteristics. For biological control, an antagonistic bacteria, N4 isolate which effectively inhibited not only mycelial growth of S2 isolate but also suppress sclerotinia rot on the pot assay, was selected and identified as Bacillus megaterium according to Bergey's manual and API system., Wettable powder type, N4 formulation using B. megaterium N4 isolate was developed and estimated its control effect on perilla crops in a plastic house. As a results, N4 formulation which applied before 3 days inoculation of pathogen was effectually controlled Sclerotinia rot as the control value of 98.0%, was more effective than chemical fungicide, benomyl showing the control value of 78.0%. This is the first report of wettable powder formulation as a biocontrol agent using B. megaterium N4 against Sclerotinia rot caused by S. sclerotiorum on perilla.

Reproductive Cycle of the Korean Perch, Coreoperca herzi (Perciformes: Centropomidae) (한국산 꺽지 Coreoperca herzi (농어목 꺽지과)의 생식주기)

  • Lim, Sang-Koo;Kim, Gye-Won;Chung, Gyu-Hwa;Han, Chang-Hee;Kang, Ki-Young;Kim, Jung-Woo
    • Korean Journal of Ichthyology
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    • v.23 no.4
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    • pp.261-268
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    • 2011
  • To clarify the annual reproductive cycle of the Korean perch, Coreoperca herzi, the seasonal changes in gonadosomatic index (GSI), hepatosomatic index (HSI), histological aspects of gonad and liver, and plasma levels of sex steroid hormones were investigated from June 1994 to April 1996. The annual variations of GSI and HSI were positively related to the plasma levels of sex steroid hormones. Estradiol-$17{\beta}$ (E2) and testosterone levels were raised during the April to May. Based on the related results, annual reproductive cycle of the fish could be divided into five successive stages; 1) Growing stage (from February to March: GSI was increased rapidly and oocytes with yolk vesicle was increased. Nucleus migrates toward the animal pole. Spermatids were activated from the epithelial tissue of lobuli). 2) Maturation and spawning stage (from April to June: Oocytes were accumulated yolk globules. Active spermatogenesis was observed). 3) Degeneration or stagnation phase (from July to August). 4) Recovery phase (from September to November) and 5) resting phase (from December to January). The main spawning period was in May.