• Applied Microscopy
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• v.33 no.2
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• pp.105-116
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• 2003

In Vitro fertilization of U. unicinctus eggs observed by immunofluorescence and electron microscopes revealed an overview of the meiotic pattern of the tide animals. The eggs have been fertilized early at germinal vesicle stage, followed by germinal vesicle break down (GVBD), but pre-mitotic aster like structure could not be resolved by the methods employed in this work. The meiotic features, such as rotation-shift movement of spindle fibers, behavior of spermatozoonmonaster in the egg cytoplasm and active spindle fiber of the 1st polar body, have been observed. The antitubulin-FITC fluorescence show the 2nd meiotic apparatus appeared firstly parallel to the tangential line of the oolemma, proceeding the meiosis, its bipolarity is rotated and shifted towards the oolemma. The polar bodysite of the oolemma was not amorphous, but active in a sense of anti-tubulin-FITC reactions during the extrusions of the polar bodies. The immunofluorescence reactions of the spermatozoon centriole appeared at a later stage of the 2nd meiosis. During the time periods, the fertilized spermatozoon resided in the egg cytoplasm. Activating the centrioles, spermatozoon approaches towards the chromosomal materials of the 2nd oocyte. This suggests that spermatozoon centrioles initiate and play a roll to fuse male and female pronuclei.

• Korean Journal of Microbiology
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• v.35 no.4
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• pp.258-265
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• 1999

Entry into meiosis in the yeast Saccharomyces cerevisiae is regulated by two major factors: the cell type MATa/MAT${\alpha}$ and the nutriational state (starvation) of the cell. The two independent regulations act through IME1and IME2 expression to initiate meiosis. IME2 encodes a meiosis-specific protein kinase, and it enabled MATa/MAT${\alpha}$ diploid cells to undergo meiosis and sporulation. The PCR mutagenesis method was applied for the isolation of thermosensitive ime2 mutants. Among sixty two mutants isolated from the phenotype of defective spore formation under the restrictive temperature, three with the most easily observed temperature-sensitive phenotype (ts ${\cdot}$ime2-11, ts ${\cdot}$ime2-12 and ts ${\cdot}$ime2-13) were selected for further study. To understand the detailed functions of IME2, we examined the defects of these mutants in the early meiotic pathway including the premeiotic DNA replication and exhibited decreased level in meiotic recombination. These results suggest that the IME2 gene plays essential role in meiotic recombination pathway as well as premeiotic DNA replication. As the result of the IME2 overexpression in ${\Delta}$mre4. moreover, it was suggested that the IME2 and MRE4 genes act on the same pathway of initiation step in meiotic recombination.

• Proceedings of the KIEE Conference
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• 2000.07d
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• pp.2696-2698
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• 2000

본 연구에서는 실수형 염색체들로 구성된 개체에 대해 감수분열을 적용하여 개체를 만들고, 이 생식체들의 랜덤한 선택과 교배에 의해 세대가 진화함에 따라 탐색을 수행하는 감수분열 유전알고리즘을 이용하여 퍼지모델의 최적 구조와 파라미터를 탐색하고 Gradient Descent 알고리즘으로 파라미터를 정밀 조정하는 방안을 제안한다. 제안된 방안을 적용하여 Box-Jenkins의 가스로 데이터에 대한 퍼지모델을 구성하고 그 적용 가능성을 보인다.

• Journal of Life Science
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• v.8 no.1
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• pp.60-66
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• 1998

The mototic and meiotic chromosomal characteristics of ICR mice were investigated with G-and C-banding techniques. For the puposes, the chromosomal preparations were made with the modified air-drying method of Imal et al. Chromosomal analysis using testis could be observed mitotic as well as meitotic chromosomal behaviors, and the centromeric regions of all chromosomes including X chromosome were strongly stained in C-banded preparations. Nineteen autosomal bivalents and a single uniequal terminally associated X-Y bivalent in normal cells were observed during the late prophase and the metaphase of the meiosis I. The mean frequencies of previously dissociated X-Y chromosomes in the primary apermatocytes of the control group were 7.45%, but the frequencies of X-Y dissociation in the alkylating agents-treated group were about 3-4 times higher than that in the control group. Application of C-banding in meiotic stages could be certainly distinguish between vibalent type and univalents type of sex chromosomes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.402-408
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• 2002

Rice cultivar 'Janganbyeo' was cultivated by irrigating the saline waters of high salinity (3.0%) and medium saliniy (1.5%) for 4 days, and low salinity (0.5%) for 30 days at tillering, early meiosis and heading stage. Leaf injury due to salinity was most severe at tillering stage in 1999, but at heading stage in 2000. Heading date was delayed by 1 to 5 days by treatment of saline waters only at tillering stage. Culm length and panicle length were most severely shortened by treatment at early meiosis stage. Yield and yield components except for panicle number were decreased most by high salinity treatment regardless of growth stages. In particular, ripening ratio and grain weight among the yield components were decreased most conspicuously by the saline water treatment regardless of salinity and growth stage. Regarding grain weight grain-filling rate and duration, there is no remarked difference among the concentrations and treatment durations of saline water at tillering stage. However, their reductions were very different among the concentrations and treatment durations of saline water at early meiosis stage, being greatest when treated with high salinity for 4 days and followed by low salinity for 30 days. Also their reductions were very severe only when treated with high salinity for 4 days at heading stage.

• Korean Aquaculture
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• v.13 no.2
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• pp.38-49
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• 2001

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.6
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• pp.2124-2128
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• 2010

In this study, we determined effects of the mitogen-activated protein kinase (MAPK) inhibitor, U0126 on meiotic maturation, microtubule organization and actin filament assembly in the porcine oocyte. The phosphorylated MAPK was first detected at 12 h after the initiation of maturation cultures, fully activated at 24h, and remained until metaphase II. Treatment of germinal vesicle (GV) stage oocytes with $20{\mu}M$ U0126 completely blocked MAPK phosphorylation, but germinal vesicle breakdown (GVBD) was normally proceeded. However, the oocytes didn‘t progress to the metaphase I. The inhibition of MAPK resulted in abnormal spindles. In oocytes treated with U0126 after GVBD, polar body extrusion was normal, but the organization of the metaphase plate and chromosome segregation were abnormal. In conclusion, MAPK activity plays an important regulatory role in GV chromatin configuration and meiotic progress in porcine oocyte maturation.

• The Transactions of the Korea Information Processing Society
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• v.7 no.6
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• pp.1844-1851
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• 2000

In this paper, a metamorphosed genetic algorithm based on the meiosis for human's chromosome is presented. In the algorithm, chromosomes in an individual are divided in half and in the other are divided into other rate. By our definition, they are composed of gametes with X-type chromosomes or Y-type chromosomes or especially M(mutation)-type chromosomes. When tow gametes among them are randomly selected and recombined, the new individual is correspondingly generated. Without reducing the searching space significantly, the global solution can be readily searched by new generated individual. The performance of he presented algorithm is examined and evaluated through proper simulation using test functions.

• Journal of The Korean Association For Science Education
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• v.31 no.5
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• pp.680-693
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• 2011

In conceptual ecology, a concept does not exist independently but occupies an ecological niche in ecological environment. Among many biological concepts, genetic concepts are connected to several units including the genetics unit, and within the genetics unit the concepts of sub-areas are highly dependent on one another. For this reason, we analyzed conceptual diversity and conceptual proximity of genetic concepts through the ecological niche approach. For this purpose, we surveyed 995 9th graders. The areas covered in the survey were four genetic concepts: gene, chromosome, mitosis, and meiosis. The questionnaire presented biological concepts or terms related to each area, and the respondent marked the relevance between the presented biological concepts or terms and each area on a scale of 1~30 points. With 9th grade students, we analyzed the change of genetic concepts through class by the ecological niche approach. Through class, the total number of concepts increased in all of the areas, and the increase was smallest in the area of meiosis followed by mitosis, chromosome and gene. Relative density decreased with increases in the number of concepts. The conceptual diversity index also increased through class in all of the areas, and the increase was smallest in the area of meiosis followed by mitosis, chromosome and gene. In addition, difference in the relative density of concepts was reduced after class, and difference in the score of relevance was also reduced and consequently similarity among concepts increased. From these results were drawn conclusions as follows: First, through class, the conceptual diversity of genetic concepts increased. Second, through class, the conceptual proximity of genetic concepts increased.

• Clinical and Experimental Reproductive Medicine
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• v.36 no.4
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• pp.231-236
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• 2009