• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.958-964
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• 1997

The three medicinal herbs-Curcuma longa Linne, Paeonia japonica Miyabe, Scutellaria baikalensis George-irradiated with gamma rays have been tested for their possible genotoxicity. The methanol-soluble and water-soluble fractions of the 10kGy gamma-iradiated herbs were examined in the Salmonella typhimurium histidine reversion assy(Ames test) using S. typhimurium TA98, TA100 and TA102 as tester strains. No mutabenicity was detected in this assay with or without metabolic activation. The safety of the herbs irradiated with gamma rays at practical doses needs to be evaluated in further tests of genotoxicity in vivo and chronic and reproductive toxicity.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.215-219
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• 2010

We have subjected to gamma-irradiation to citrus buds and then grafted onto mature citrus tree. Mutant citrus branch lines have been induced. As a result of first selection, we found the several mutant lines showing interesting phenotypes such as higher sugar content. We have selected several branches showing good qualities such as higher sweetness and/or lower acidity. Some branch lines showed over $13^{\circ}Brix$ sugar content and below 0.9% acidity. Other mutant branch lines showed the changes of shape, size, peel thickness, and fiber contents or distribution of fruits. The results suggest that gamma-irradiation is an effective tool for induction of citrus mutant lines.

• Korean journal of food and cookery science
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• v.17 no.6
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• pp.617-624
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• 2001

Gamma irradiation (1-10 kGy) was applied to chicken for the evaluation of their microbiological safety and possible genotoxicity. In 3 kGy-irradiated sample, the growth of psychrophile was inhibited about 1.5 log cycles and no cells were recovered in total microbial counts. All kinds of contaminated microorganism were sterilized by 7 kGy-irradiation. Also, irradiation followed by freeze-storage at the same time was very effective in inhibiting bacterial growth. The genotoxicity of 10 kGy-irradiated chicken was evaluated by Salmonella Typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535, and TA1539. Clastogenic effects were not shown in vivo mouse micronucleus assay at 10 kGy-dose tested.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.75-79
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• 2001

Quality changes in sausage stuffed into irradiated natural pork and lamb intestine were studied. Total aerobic bacteria of the sausage prepared with irradiated natural pork and lamb casing were significantly lower than those of nonirradiated casing and the $D_{10}$ value of those sausages were 1.71 and 1.58 kGy, respectively. The sausages in pork intestine vacuum-packaged and stored in a $4^{\circ}C$ refrigerator showed negligible change in the 2-thiobarbituric acid reactive substances (TBARS) value during storage except for 10-day when the TBARS value was higher than other storage days. Total working force for shear value of the sausages were lower with both irradiated casings but sensory evaluation showed no difference between treatments. As a results, the gamma irradiation was effective for sanitizing the natural pork and lamb casings, thereby preparing high quality sausages.

• Food Science and Preservation
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• v.8 no.1
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• pp.54-59
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• 2001

The three medicinal herbs, Astragali Radix, Glycyrrhizae Radix and Aurantii nobilis Pericarpium, irradiated with γ-rays have been tested for their possible genotoxicity. The hot water extracts of the 10 kGy γ-ray irradiated herbs were examined in the Salmonella mutagenicity test(histidine reversion assay; Ames test) using Salmonella. typhimurium TA98 and TA100 as tester strains. No mutagenicity was detected in this assay both with and without metabolic activation. The safety of the herbs irradiated with γ-rays at tactical doses needs to be evaluated in further tests of genotoxicity in vivo and chronic and reproductive toxicity.

• Journal of the East Asian Society of Dietary Life
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• v.8 no.4
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• pp.430-440
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• 1998

Meat is a perishable product since it offers favorable conditions for microbial contamination and multiplication. Besides, undercooked and raw meat has been linked to outbreaks of hemorrhagic diarrhea due to the presence of microbial pathogens. Therefore, this study was conducted to find out the effect of the use of gamma-irradiation in order to improve preservation and eliminate this microorganisms. The Initial level of microbial contamination in pork was 1.1$\times$10$^3$ CFU/g (aerobic bacteria), 5.3$\times$10$^3$CFU/g (psychrophile), 3.1$\times$10$^3$ CFU/g (coliforms) and 3.2$\times$10$^3$ CFU/g(Listeria), All kinds of contaminated microorgnisms were sterilized by gamma irradiation at 3 kGy. The proximate composition of pork was not significantly changed by irradiation dose and storage period. The pH was slightly increased during storage period and titratable acidity decreased. However, no significant changes in pH and acidity were observed by gamma irradiation. There was no difference in total amino acid content regardless of irradiation dose and storage period. Glutamic acid was detected at high levels throughout the storage period. The major mineral compositions of pork were potassium, phosphorus, sodium, magnesium. No significant difference in the components of minerals were observed by gamma irradiation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1137.2-1245
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• 2001

The ${\gamma}$-irradiated medicinal herbs were examined the genotoxicological safety to consider the possibility of application of the irradiation technology for hygienic purpose. The three medicinal herbs -Glycyrrhigae Radix, Aurantii nobilis Pericarpium and Bupleuri Radix- were irradiated with ${\gamma}$ -rays at the practical dosage of 10 kGy. The hot water extracts of the irradiated herbs were examined in two short-term in vitro tests: (1) Ames test in Salmonella typhimurium TA98 and TA100, (2) Micronucleus test in cultured Chinese hamster ovary(CHO) cells. In the Salmonella reversion assays both with and without metabolic activation, the number of revertant colonies was not increased with each extract from the irradiated herbs, compared with negative controls. No significant difference in formation of the colonies was observed between non-irradiated and 10 kGy-irradiated herbs. These results indicated that no mutagenicity of the irradiated herbs was detected. In the micronucleus tests in cultured CHO cells, the incidences of micronucleus were not increased with irradiated herbs, and no significant difference in the incidences was observed between non-irradiated and irradiated herbs. These results indicated that no cytogenetic toxicity of the irradiated herbs was detected. The results of the two in vitro tests suggest that the irradiated herbs do not show mutagenic effects and cytogenetic toxicity. Further tests of in vivo genotoxicity and chronic toxicity are needed to determine the safety of the herbs irradiated with ${\gamma}$ -rays at practical doses.

• Korean Journal of Environmental Agriculture
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• v.18 no.3
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• pp.245-249
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• 1999

This study was carried out to investigate the influence of artificial acid rain on the growth of soybean irradiated with the low dose gamma radiation. The growth patterns of Hwangkumkong and Bangsakong were not dependent on the acid rain treatment and the period of seed storage but the radiation dose. In Hwangkumkong, the optimal irradiation dose for the growth promotion was lower in the acid rain treatment group than the control group. The symptom of acid rain damage appeared broadly around pH 2.20, were higher in Hwangkumkong than in Bangsakong, and were more effective in the seeds of 1997 than 1995. But the damage symptom of Bangsakong was not dependent on the acid rain treatment and the irradiation of gamma radiaiton.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.429-438
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• 2014

This study was designed to investigate the effects on anti-oxidative activities and increasing extraction yield of Aralia elata Cortex by gamma irradiation. Electron spin resonance (ESR) analysis as physical techniques for irradiation identification of Aralia elata showed that a pair of peak appeared on a space of 6.0 mT at the left and right of symmetric unspecific central ESR spectrums, confirming that the plant was gamma-irradiated. The optimum extracting conditions for preparing gamma irradiated samples from Aralia elata Cortex were to extract with 50% ethanol for 15 hrs after 10 kGy irradiation. DPPH scavenging activity and ABTS radical cation inhibitory activity of the water and 50% ethanol extracts from non irradiated and irradiated Aralia elata Cortex was very high as over 80% and 98%, respectively, at tested low concentration of $50{\mu}g/mL$. Antioxidant protection factor (PF) as anti-oxidation indicator of lipophilic compounds showed a very high level of activity as 2.18~2.78 PF. As for TBARs, water and ethanol extracts showed high level. Increase of TBARs inhibitory activity of water extracts was not shown by gamma-ray irradiation but ethanol extracts showed slight increasement of TBARs inhibitory activity with 10 kGy gamma-ray irradiation. These results shown confirmed increasement of extraction yield for phenolic compounds and anti-oxidative activity from Aralia elata. Thus, the treatment of gamma-irradiation can be used a way to amplify a solubility for biological active compounds and anti-oxidative activity in plants.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1788_1789
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• 2009

본 논문에서는 핵폭발과 같은 고준위방사선환경에서의 전자부품소자의 피해평가분석을 통하여 군용전자장비의 내방사선화를 하기 위한 기반기술의 확립을 위한 연구의 일환으로 74AC04(Inverter) IC에 대한 고준위감마선조사시험을 통하여 Co-60 Gamma-ray 선원을 사용하여 총 400Krad[si] 누적 선량에 대한 74AC04 소자의 동작특성 및 전기적 파라메터의 변화분석을 진행하였다. 시험평가 방법 및 절차는 MIL-STD-883G 1019.7[1] 및 ESA/SCC Basic Specification No.22900[2] 절차를 기준으로 하여 동일 lot에 대한 5개의 샘플을 이용하여 동작특성에 영향을 미치는 주요한 전기 적파라메터인 정지소비전류, 입력누설전류, VIL(Maximum Low Level Input Voltage)에 대한 변화추이를 분석하였다. 이번 조사시험을 통하여 입력게이트에서의 누적선량에 따른 TID(Total Ionizing Dose) 효과로 인한 VIL의 감소 추이를 확인 할 수 있었으며 총 누적선량 160Krad 이상에서의 VIL은 허용기준치이하로 감소하였고 정지소비전류의 경우 누적선량에 따른 점진적 증가 현상과 200Krad부근에서의 설계스펙허용치를 초과하는 결과를 확인하였다.