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http://dx.doi.org/10.7732/kjpr.2014.27.5.429

The Effect on Anti-oxidative Activity and Increasing Extraction Yield of Aralia elata Cortex by Gamma Irradiation  

Park, Hye-Jin (Natural F&P Co., Ltd.)
Cho, Young-Je (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University)
Publication Information
Korean Journal of Plant Resources / v.27, no.5, 2014 , pp. 429-438 More about this Journal
Abstract
This study was designed to investigate the effects on anti-oxidative activities and increasing extraction yield of Aralia elata Cortex by gamma irradiation. Electron spin resonance (ESR) analysis as physical techniques for irradiation identification of Aralia elata showed that a pair of peak appeared on a space of 6.0 mT at the left and right of symmetric unspecific central ESR spectrums, confirming that the plant was gamma-irradiated. The optimum extracting conditions for preparing gamma irradiated samples from Aralia elata Cortex were to extract with 50% ethanol for 15 hrs after 10 kGy irradiation. DPPH scavenging activity and ABTS radical cation inhibitory activity of the water and 50% ethanol extracts from non irradiated and irradiated Aralia elata Cortex was very high as over 80% and 98%, respectively, at tested low concentration of $50{\mu}g/mL$. Antioxidant protection factor (PF) as anti-oxidation indicator of lipophilic compounds showed a very high level of activity as 2.18~2.78 PF. As for TBARs, water and ethanol extracts showed high level. Increase of TBARs inhibitory activity of water extracts was not shown by gamma-ray irradiation but ethanol extracts showed slight increasement of TBARs inhibitory activity with 10 kGy gamma-ray irradiation. These results shown confirmed increasement of extraction yield for phenolic compounds and anti-oxidative activity from Aralia elata. Thus, the treatment of gamma-irradiation can be used a way to amplify a solubility for biological active compounds and anti-oxidative activity in plants.
Keywords
Anti-oxidative activity; Solubility; Aralia elata Cortex; Extracts; Gamma irradiation;
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