• Applied Microscopy
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• v.36 no.3
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• pp.141-153
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• 2006

The purpose of this study is to find out how soft contact lens multi-purpose solution (MPS), often used for medical treatment, effects the inhibition on cell growth and research the result of using MPS, suspected to demage eye cells, on rabbit eye's corneal epithelium and endothelium tissue. In this treatise, $ReNu^{(R)}$ (Baush & Lomb, USA), Opti-free $express^{(R)}$ (Alcon, USA), Free-sol $plus^{(R)}$ (Hanamedicon, Korea) had been selected among the MPS. After culturing L929 roil line, cell growth inhibition rate was measured by MTT assay, and by making Hematoxylin and Eosin stain specimen. the morphology was observed by optical microscope. In the In vivo experiment,9 white rabbit eyes (18 eyes) were classified into 3 groups. The experimental group is left eyes (9 eyes) of rabbit, and MPS were dropped; however. the control group, the right eyes (9 eyes), were only used a saline solution without preservatives. After the dropping within the period, the cornea surface of rabbit eyes were stained by Rose bengal and observed. To figure out the changes of the corneal epithelium and endothelium tissue scanning electron microscopy (SEM) has been used. As the result, the rate of cell growth inhibition was 54%. 73% and 36%, respectively. Morphological changes represented that the shape has been changed into oval or round shape and those are not considered as a common formation of L929 cell line. When it comes to staining Rose bengal, each experiment group was stained red which is not shown in controls. The polygonal mosaic pattern of a corneal epithelium was disturbed in the picture taken by SEM; furthermore, the shape of the corneal endothelium was irregular. In conclusion, as we consider antimicrobial effect and the safety on living cells, it is necessary that we should improve concentration of preservatives and study continuously to develop a new preservatives without a toxic effect on the cornea surface.

• Journal of The Korean Ophthalmological Society
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• v.59 no.11
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• pp.1049-1055
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• 2018

Purpose: To investigate the factors associated with glaucoma in patients with pseudoexfoliation syndrome by comparing features of the anterior segments and ocular biometry according to the presence or absence of open-angle glaucoma in pseudoexfoliation syndrome. Methods: We analyzed 96 patients (115 eyes) diagnosed as having pseudoexfoliation syndrome in this study. The patients were divided into two groups of simple pseudoexfoliation syndrome (64 patients, 76 eyes) and pseudoexfoliation glaucoma (32 patients, 39 eyes). We compared the age, sex, underlying disease, location of pseudoexfoliative material, iris change, degree of nuclear cataract, pupil dilatation, corneal endothelial cell counts, central corneal thickness, anterior chamber depth, axial length, corneal curvature, and intraocular pressure (IOP). Results: There were no significant differences between the two groups in terms of age (p = 0.694), sex (p = 0.161), diabetes (p = 0.440), hypertension (p = 0.238), pseudoexfoliative material observed in anterior capsule (p = 0.700), pupillary margin (p = 0.210), iris depigmentation (p = 0.526), pupillary ruff loss (p = 0.708), degree of nuclear cataract (p = 0.617), pupil dilatation (p = 0.526), central corneal thickness (p = 0.097), anterior chamber depth (p = 0.283), axial length (p = 0.095), or horizontal and vertical corneal curvature (p = 0.066 and 0.306, respectively). In pseudoexfoliation glaucoma, significantly higher IOP (p = 0.026), a high frequency of membrane formation (p = 0.047), and decreased corneal endothelial cell counts (p = 0.048) were observed. Conclusions: Pseudoexfoliation syndrome with open-angle glaucoma was shown to be associated with high IOP, decreased corneal endothelial cell counts, and a high frequency of membrane formation. Therefore, when such changes are observed in pseudoexfoliation syndrome patients, a higher risk of open-angle glaucoma should be recognized, and careful attentionis required accordingly.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.3
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• pp.102-112
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• 2013

In this paper, two step processing method of contour extraction and complement which contain hexagonal shape for low contrast and noisy images is proposed. This method is based on the combination of Laplacian-Gaussian filter and an idea of filters which are dependent on the shape. At the first step, an algorithm which has six masks as its extractors to extract the hexagonal edges especially in the corners is used. Here, two tricorn filters are used to detect the tricorn joints of hexagons and other four masks are used to enhance the line segments of hexagonal edges. As a natural image, a corneal endothelial cell image which usually has regular hexagonal form is selected. The edge extraction of hexagonal shapes in corneal endothelial cell is important for clinical diagnosis. The proposed algorithm and other conventional methods are applied to noisy hexagonal images to evaluate each efficiency. As a result, this proposed algorithm shows a robustness against noises and better detection ability in the aspects of the output signal to noise ratio, the edge coincidence ratio and the extraction accuracy factor as compared with other conventional methods. At the second step, the lacking part of the thinned image by an energy minimum algorithm is complemented, and then the area and distribution of cells which give necessary information for medical diagnosis are computed.

• Journal of Korean Ophthalmic Optics Society
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• v.10 no.4
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• pp.283-292
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• 2005

This study investigated the effects of naringin, and chitosan in rabbits' corneas. Naringin, a glycone of naringenin, is a widely distributed bioflavonoid in the grapefruit and citrus peel, and it has already been reported as an antioxidant, antimicrobial, and anticancer agent. It has been used as a food preservatives and cosmetics. One of the natural preservatives, chitosan has also used in food preservatives, health drinks, and teas. Chitosan is distributed in the epithelium of crustacea, insects, and fungi. Naringin and chitosan have no harmful effects of cytotoxicity in the human body and they are recognized as an antibacterial for various forms of bacteria. The purpose of this study is to search for the ideal percentage of natural products to substitute the chemical preservatives occuring within the cornea and conjunctiva cytotoxicity and inflammations as wearing on soft contact lens. The present study compared the morphology of corneal epithelium and endothelium observed by light microscopy (LM) and transmission electron microscopy (TEM). In vivo methods, We investigated the effects of natural preservatives on soft contact lens. We inserted 3-4 drops of the naringin and chitosan, directly on rabbits' corneas 4 times per a day during one week. After enucleation of cornea, morphorgical damages of the epithelium and endothelium were observed by LM and TEM. In view of ultrastructure, chitosan caused siginficant damage on the epithelium and endothelium of cornea. The damage of cells was higher in chitosan treated cornea than 0.01, 0.1, and 1% of naringin. The 1% of naringin also expressed cell damage seriously. The results suggest that the most important thing is to use the reasonable percentage of preservatives for contact lens solutions.

• Applied Microscopy
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• v.36 no.3
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• pp.227-234
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• 2006

To investigate the comparative effect of spherical and aspherical RGP lens were worn during 3 weeks on rabbit's cornea. Four white rabbits were worn right eyes with spherical lens and 4 white rabbits were worn right eyes with aspherical RGP lens. Left eyes were served as control. The rabbits were sacrificed at 3 weeks after fitting and observed morphological changes by scanning electron microscopy and also investigate proliferation rate of the corneal epithelium with RGP wearing. After spherical RGP lens wearing, the epithet layer damaged compared to aspherical lens. The superficial cell layer strip off seriously, cell size significantly changed abnormal. Both spherical and aspherical RGP lens fitting group showed so many bacteria and back surface of lens was found like a fern shape. The aspherical RGP lens original material type was some formal than spherical lens. We thought that these pattern was significantly altered with spherical lens by prohibited transmitter oxygen from atmosphere therefore the epithelium shape was changed. This suggested wearing the aspherical lens might be less physiologic than shperical lens fitting.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.411-414
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• 2010

Rabbit's cornea is the most common thing that used for researches in ophthalmology, so many experimentations have done. The purpose of this study was to survey the corneal pachymetry, tonometry and specular microscopy in New Zealand white rabbits. Three months old male rabbits ($2.9{\pm}0.2\;kg$) were examined with contact the specular microscope to evaluate the endothelial cell density, coefficiency of variation and the percentage of hexagonal cells in the central cornea. For the pachymetry, ultrasonic pachymeter was used for central cornea in each twenty rabbits, and tonometry was performed forty rabbit's eyes to evaluate the intraocular pressure. Corneal endothelial cell density in New Zealand white rabbit was $3304{\pm}354\;cell/mm^2$. Coefficiency of variation was $45.8{\pm}2$ and the percentage of hexagonal cells in the central cornea was $44.4{\pm}11%$. The pachymetry of cornea was $346.1{\pm}21\;{\mu}m$ and tonometry of eye was $14.3{\pm}2\;mmHg$. It is considered that normal New Zealand white rabbit's pachymetry, specular microscopy and tonometry were useful basic data of rabbit's cornea for research and clinic in veterinary ophthalmology.