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The Cytotoxicity and Effect on the Experimental Rabbit Cornea of Soft Contact Lens Multi-Purpose Solution (MPS)  

Koh, Eun-Gyeong (Department of Biology, College of Natural Science, Chonnam National University)
Chae, Soo-Chul (Department of Biology, College of Natural Science, Chonnam National University)
Park, Su-Kyoung (Department of Biology, College of Natural Science, Chonnam National University)
Kim, Deok-Song (Department of Biology, College of Natural Science, Chonnam National University)
Lee, Jong-Bin (Department of Biology, College of Natural Science, Chonnam National University)
Publication Information
Applied Microscopy / v.36, no.3, 2006 , pp. 141-153 More about this Journal
Abstract
The purpose of this study is to find out how soft contact lens multi-purpose solution (MPS), often used for medical treatment, effects the inhibition on cell growth and research the result of using MPS, suspected to demage eye cells, on rabbit eye's corneal epithelium and endothelium tissue. In this treatise, $ReNu^{(R)}$ (Baush & Lomb, USA), Opti-free $express^{(R)}$ (Alcon, USA), Free-sol $plus^{(R)}$ (Hanamedicon, Korea) had been selected among the MPS. After culturing L929 roil line, cell growth inhibition rate was measured by MTT assay, and by making Hematoxylin and Eosin stain specimen. the morphology was observed by optical microscope. In the In vivo experiment,9 white rabbit eyes (18 eyes) were classified into 3 groups. The experimental group is left eyes (9 eyes) of rabbit, and MPS were dropped; however. the control group, the right eyes (9 eyes), were only used a saline solution without preservatives. After the dropping within the period, the cornea surface of rabbit eyes were stained by Rose bengal and observed. To figure out the changes of the corneal epithelium and endothelium tissue scanning electron microscopy (SEM) has been used. As the result, the rate of cell growth inhibition was 54%. 73% and 36%, respectively. Morphological changes represented that the shape has been changed into oval or round shape and those are not considered as a common formation of L929 cell line. When it comes to staining Rose bengal, each experiment group was stained red which is not shown in controls. The polygonal mosaic pattern of a corneal epithelium was disturbed in the picture taken by SEM; furthermore, the shape of the corneal endothelium was irregular. In conclusion, as we consider antimicrobial effect and the safety on living cells, it is necessary that we should improve concentration of preservatives and study continuously to develop a new preservatives without a toxic effect on the cornea surface.
Keywords
Rabbit cornea; Cytotoxicity; Soft contact lens Multi-Purpose Solution (MPS); Preservatives;
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1 Dawson NM, Brown TJ, Till DG: The effect of baquacil on pathogenic free-living amoebae: NZJ Mar Freshwater Res 17 : 305-311, 1983   DOI
2 Duguid IGM, Dart JKG, Morlet N, Allan BDS, Matheson M, Ficker L, Tuft S: Outcome of Acanthamoeba keratitis treated with Polyhexamethylene Biguanide and Propamidine. Ophthalmology 104 : 1587-1592, 1997   DOI   ScienceOn
3 Gilbert P, Pemberton D, Wilkinson DE: Barrier properties of the gram-negative cell envelope towards high molecular weight polyhexamethylene biguanides. J Appl Bacteriol 69 : 585-592, 1990a   DOI
4 Robert B: Lens handling, care and storage. Contact lens practice. 568-597, 1988
5 Stehrgreen JK, Bailey TM, Visvesvara GS: The epidemilolgy of Acanthamoeba keratitis in the United States. Am J Ophthalmol 107 : 331-336, 1989   DOI
6 Codling CE, Naillard JY, Russell AD: Asepects of the antimicrobial mechanisms of action of a polyquaternium and an amidoamine. J Antimicrob Chemother 51 : 1153-1158, 2003   DOI   ScienceOn
7 Ribasi F, Longanesi L, Casolari C: Cytologic diagnosis of Acanthamoeba keratitis. Report at a case with correlative study with indirect immunofluorescence and scanning electron microscopy. Acta Cytol 39 : 821-826, 1995
8 Francois D, Rita L: Rapid colorimetric assay of cell growth and survival. J Immunol Methods 89 : 271-277, 1986   DOI   ScienceOn
9 Larkin DRP, Kilvington S, Dart JFG: Treatment of Acanthamoeba keratitis with polyhexamethylene biguanide. Ophthalmology 99 : 185-191, 1992   DOI
10 Mosmann T: Rapid colorimetric assay for cellular growth and survival : Application of proliferation and cytotoxicity assays. J Immunol Methods 65(1-2) : 55-63, 1983   DOI   ScienceOn
11 Stephan R, Ulrike B: Cytotoxicity assay, Growth inhibition test report. ANAWA, International Bioscience Test 931043 : 1-2, 1993
12 Ikeda T, Ledwith A, Bamford CH, Hann RA: Interaction of polymeric biguanide biocide with phospholipid membranes. Biochem Biophys Acta 769 : 57-66, 1984   DOI   ScienceOn
13 Kilbington S: Activity of water biocide chemicals and contact lens disinfectants on pathogenic free libing amoebae. Int Biodeterior 26 : 127-138, 1990   DOI   ScienceOn
14 Rosenthal RA, McAnally CL, McNamee LS, Buck SL, Schlitzer RL, Stone RP: Broad spectrum antimicrobial activity of a new multi-purpose disinfecting solution. CLAO J 26 : 120-126, 2000
15 Yoon Y, You GC, Kim JM, Ra MS, Lee JB: The inhibitiory effect of multi-purpose solution on HCE and L929 cells. Korean J Vis Sci 1 : 81-88, 1999. (Korean)
16 Ryu KH, Suk DJ, Oum BS: Effects of hydrogen peroxide on rabbit corneal bioelectric properties. J Korean Ophthalmol Soc 36 : 141-152, 1995. (Korean)
17 Michael JA, Andrew PM, Graham FW: Cooperativity in the binding of the cationic biocide polyhexamethylene biguanide to nucliec acids. Biochemical & biophysical Res 318 : 397-404, 2004   DOI   ScienceOn
18 Pfister RR, Burstein N: The effect of ophthalmic drugs, vehicles, and preservatives on corneal epithelium : A scanning electron microscope study. Invest Ophthalmol 15(4) : 246-259, 1976
19 The IACLE contact lens course. MODULE 5, IACLE (International Association of Contact Lens Educators), 70 : 1997
20 Tripathi BJ, Tripathi RC, Kolli SP: Cytotoxicity of ophthalmic preservatives on human corneal epithelium. Lens & Eye Tox Res 9 (3 & 4) : 361-375, 1992
21 Reanne H, John D and Simon K: Activity of the amidoamine myristamidopropyl dimethylamine against keratitis pathogens. J of Antimicrobial Chemotherapy 51 : 1415-1418, 2003   DOI   ScienceOn
22 Desmond FM, Meridith R, Robert T, Lewis W: The IACLE Contact Lens course Module 5 : First Edition, Australia, pp. 37-134, 1988
23 Caroline D, Francoise B, Pierre-Jean P: Quarternary Ammonium and Other Preservatives' Contribution in Oxidative Stress and Apoptosis on Chang Conjunctival Cells. Investigative Opthalmology & Visual Science 42(3) : 642-652, 2001
24 Gennaro AR: Remington's Pharmaccutical Sciences, 17th edition, Mack Publishing Co, Pennsylvania 88 : 1159-1160, 1169, 1985
25 Lynn F: USP extraction of contact lenses in saline. Standard operating procedure. 1997
26 Gilbert P, Pemberton D, Wilkinson DE: Synergism within polyhexamethylene biguanide biocide formulations. J Appl Bacteriol 69 : 593-598, 1990b   DOI
27 Broxton P, Woodcock PM, Heatley F, Gilbert P: Interaction of some polyhexamethylene biguanides and membrane phospholipids in Escherichia coli. J Appl Bacteriol 57 : 115-124, 1984   DOI