• Proceedings of the Korea Information Processing Society Conference
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• 2021.11a
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• pp.741-744
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• 2021

본 논문은 사전 학습된 심층생성모델을 기반으로 가수 별 가사의 특성을 반영하여 새로운 가사를 생성하는 모델을 소개한다. 베이스 모델로 한국어 사전 학습 모델 KoGPT-2 를 사용하였으며, 총 가수 10 명의 노래 823 곡을 수집하여 미세조정 기법을 바탕으로 학습하였다. 특히, 가수 별로 구분한 가사를 학습 데이터로 구축하여, 가수 별로 독특하게 나타나는 가사 스타일이 전이되도록 하였다. 가수의 이름과 시작 단어를 입력으로 주고 작사를 수행한 실험 결과, (i) 가수 별로 생성되는 가사의 어휘와 스타일이 그 가수의 기존 곡들의 가사와 유사함을 확인하였고, (ii) 작사 결과 가수 별 차이를 확인하였다. 추후 설문을 통해, 개별 가수들의 가사와 생성된 가사의 어휘와 스타일 유사성을 확인하고, 가수 별 차이 또한 확인하고자 한다.

• Proceedings of the Korean Society for Information Management Conference
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• 2014.08a
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• pp.7-10
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• 2014

이 연구에서는 음원 서비스의 이용자들이 공개앨범에서 추천한 음원 리스트에 특성가수와 동시출현한 곡들의 정보를 분석하여 특성가수의 이미지를 네트워크 기법으로 표현하였고 동시출현한 곡의 통계분석을 통하여 해당 가수를 선택한 이용자가 선호할 만한 연관 곡을 추천하고자 하였다. 분석결과 추천되는 음원리스트에 동시 출현되는 가수들의 장르적 특성으로 특정가수의 이미지가 표현되었고 시기별로 가수의 이미지가 변화되는 것이 추적되었다. 이 연구에서 제시된 방법은 이용자에게 변화하는 가수의 이미지에 따라 연관 정보를 유연하게 추천할 수 있는 방안으로 활용될 수 있다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.2
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• pp.77-86
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• 1990

The residue after hot water extraction of blue crab, Portunus trituberculata, was hydrolyzed for utilizing the byproducts as seasonings. The acid(5N HCl) hydrolyzates were then neutralized with $Na_2CO_3$, 5N NaOH or 5N NaOH hydrolyzate, while the alkali hydrolyzates (5N NaOH) were also neutralized with 5N HCl or 5N HCl hydrolyzate. The total nitrogen and formol nitrogen contents increased, and the platability of the hydrolyzates was also enhanced by neutralization. The released amino acid contents from the neutralized hydrolyzates with $Na_2CO_3$, 5N NaOH and 5N NaOH hydrolyzate were $2,274mg\%,\;2,105.0mg\%$ and $2,683.5mg\%$, respectively. Amino acid contents from the neutralized hydrolyzates with 5N HCl and 5N HCl hydrolyzate were $1,352.5mg\%$ and $2,498.8mg\%$, respectively. In the decolorization of hydrolyzates using decolorization agent, powdered active carbon showed good decolorizing effect. Powdered active carbon decreased total nitrogen and formol nitrogen contents in direct relationship to the increase in its concentration. The effective concentration of active carbon used as decolorization agent showed as $1\~2\%$ of the crab hydrolyzate. Salt contents could be decreased at 37 brix by desalination method such as the evaporation of the hydrolyzate contents.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.168-171
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• 2005

한우의 Myosin B 단백질을 단백질 가수분해 효소인 pepsin으로 처리한 다음 단백질의 함량과 혈압상승 펩티드 생성효소인 angiotensin-I converting enzyme(ACE)에 대한 저해활성을 측정하였다. 등심이 우둔에 비해 단백질의 함량이 높았으며, 가수분해 처리 후 우둔과 다르게 등심은 3시간 가수분해 처리구에서 단백질의 함량이 높게 나타났다. ACE 저해활성은 등심에서는 3시간, 우둔에서는 6시간동안 가수분해시켰을 때 ACE 저해율이 유의적으로 가장 높게 나타났으며, 3, 6시간동안 가수분해시켰을 경우 부위 별로 유의적인 차가 있었으나(p<0.05), 0, 1시간동안 가수분해 시켰을 때는 부위간의 유의적인 차는 없었다(p>0.05). ACE 저해율이 가장 좋은 가수분해 처리구를 ultrafiltration시킨 결과, 저분자 peptide 상태의 가수분해물이 고분자에 비하여 ACE 저해율이 높은 것으로 나타났다. 차후 ACE 억제활성도가 높은 단백질을 분리하여 가장 우수한 분획을 찾아 아미노산 염기서열을 밝혀 고혈압 억제제로 합성 개발하는 연구를 추진할 예정이다.

• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.1-8
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• 1989

Ten cultivars of malting barley grown at four locations were malted and assayed for six enzymes involved in the degradation of nucleic acids. Among these enzymes were deoxyrinonuclease, ribonuclease, phosphodiesterase, 3'- and 5'- nucleotidases and phosphomonesterase. Activities of all enzymes in five-day malts were significantly affected by variety and location of growth. The average levels of ribonuclease, deoxyribonuclease, 3'-nucleotidase and 5'-nucleotidase of 80 five-day malts were 11.2, 5.7, 5.6 and 1.2 units per gram of malt, respectively. Six-rowed barley malts contained higher levels of deoxyribonuclease, phosphodiesterase and 3'-nucleotidase than those of two-rowed barley malts, while two-rowed barley malts contained significantly higher ribonuclease levels than those of six-rowed barley malts.

• Korean Journal of Food Science and Technology
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• v.26 no.2
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• pp.110-116
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• 1994

In order to study the effects of enzyme modification on the physico-chemical and functional properties of myofibrillar protein prepared from the frozen sardine, Sardinops melanostica, the protein was hydrolyzed with pepsin under the enzyme-substrate ratio 1:100 at $37^{\circ}C$ and pH 1.65 for 1, 4, 8, 12, and 24 hr, respectively. The properties of pepsin-modified sardine myofibriliar protein were determined. The extents of proteolysis with pepsin as a fuction of time was showed a typical enzyme hydorlysis curve with an initial region of 4 hour period followed by plateau region. The SDS-acrylamide slab gel electrophoresis patterns of pepsin-modified proteins showed mainly disappearances of minor protein bands, but no changes of main protein bands. The gel filtration patterns through Sephadex G-75 of sardine myofibrillar protein showed two big peaks and three small peaks. All the small peaks were disappearanced by proteolysis with pepsin in one hour. and during the period of proteolysis the fast big peak became gradually smaller and the late big peak eluted more slowly. By proteolysis, the emulsifying activity and emulsifying capacity of sardine myofibrillar protein were all decreased. The effects of pepsin-modification on emulsifying capacity were greater than those on emulsifying activity of protein. The aeration capacity of the protein was increased about 1.9 folds and the foam stability decreased to 0.6 folds of control by pepsin-modification. The pepsin-modified sardine myofibrillar proteins showed about 0.6 folds of heat coagulation and 1.4 folds of viscosity of control. The pH dependence of solubilities of sardine myofibrillar protein showed two isoelectric areas of pH 5 and 9. The pepsin-modified protein showed more clear pH dependences at the early stage but not at the late stage of proteolysis.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.129-136
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• 2007

This study was performed to determine the protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Angiotensin-I-converting enzyme(ACE) inhibitory activity (IC50) as affected by the various meat cuts, digestion times with pepsin. Hydrolysates having the protein concentration of 10 ug/mL had approximately 36∼39% ACE inhibitory activities, regardless of meat cut and digestion time. Protein concentration and ACE inhibitory activity of the diluted hydrolysate increased after 1-hr digestion. In original hydrolysates, ACE inhibitory activities of loin had higher than those of round (P<0.05). In addition, non-heated hydrolysates had higher ACE inhibitory activities than heated counterparts. When myosin B was digested by pepsin more than 1 hr, improved ACE inhibitory activities were observed as compared to the non-digested control.

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.678-683
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• 1996

The purpose of this study was to elucidate characteristics of hydrolysates from file-fish flesh with various proteases. File-fish flesh was chopped, homogenized with water, and hydrolysed by 8 different kinds of commercially available protease. High production of peptide was observed in bromelain and neutrase treatment. On the other hand, large amount of free amino acid was observed in esp/sav and pronase treatment. Neutrase and pancreatin hydrolysate contained large amount of 5'-GMP. Organoleptic studies showed that the bromelain, esp/sav and protease hydrolysate had strong bitter taste, while pronase and esp/sav hydrolysate had strong umami taste. From these results, pronase was found to be suitable enzyme for producing file-fish hydrolysate.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.290-293
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• 2005

장내 단백질 가수분해효소에 의한 casein 분해산물들의 ACE 저해 활성을 비교하기 위하여 인공위액, 인공장액 및 인공위액과 장액 연속처리를 각각 반응조건으로 일정시간 casein을 가수분해한 후 각 효소 가수분해물이 혈압상승 peptide 생성효소인 Angiotensin converting enzyme(ACE)에 대한 저해활성을 측정하였다. 각 소화액에 따른 가수분해물은 모두 ACE 저해활성을 나타내었으며, 2시간 효소처리를 하였을 때, 인공위액, 인공장액, 인공위액과 장액 가수분해물은 각각 69%, 80% 및 88%로 최대 ACE 저해활성을 보였다. 그리고 casein의 가수분해 정도를 확인하기 위한 SDS-PAGE에서는 인공위액으로 처리한 군보다 인공장액으로 처리한 군이 더 작은 분자량으로 분해되는 것을 확인할 수 있었고, 인공위액과 장액을 함께 처리한 군에서는 1시간 안에 대부분의 분해가 이루지는 것을 관찰할 수 있었다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.304-306
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• 2004

본 연구는 산양유 ${\beta}$-casein의 효소에 의한 가수분해 특성과 가수분해물의 Angiotensin Converting Enzyme의 저해 효과를 측정하고자 실시하였으며 그 결과는 다음과 같다. 1. 산양유 ${\beta}$-casein은 Mono S HR 5/5 양이온 교환 컬럼에서 분리하여 SDS-PAGE로 순수하게 분리된 것을 확인하였다. 2. 분리된 ${\beta}$-casein을 Trypsin으로 처리하여 가수분해물의 ACE저해 활성을 측정한 결과 가수분해 하지 않은 ${\beta}$-casein은 1.8%의 저해활성을 나타낸 반면, 가수분해한 ${\beta}$-casein은 25.36%의 저해활성을 나타내어 ACE 저해활성이 크게 증가한 것을 확인하였다. 3. 가수분해물의 $IC_{50}$을 측정한 결과 $308.7{\mu}{\ell}/m{\ell}$로 나타났다.