• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.5
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• pp.730-735
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• 2013

This study compared the nutritional components (proximate components, fatty acids, amino acids, minerals and vitamins) between genetically modified (GM) drought-tolerant rice and a parental rice cultivar (Ilmibyeo) as a non-GM control. Both GM and non-GM rices were grown and harvested in two different locations, Gunwi and Suweon in Korea. Proximate components (moisture, starch, protein, lipid, and ash contents) were similar between the drought-tolerant GM rice and the conventional non-GM rice. There were no significant differences between the GM and non-GM rice in most of their nutrient compositions, despite minor locational differences of some amino acids and minerals. These results indicate that transgenic rice with a genetically improved resistance to drought is equivalent to the parental rice cultivar without major changes in its chemical contents.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.5-5
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• 2020

가뭄은 일반적으로 기상학적, 수문학적, 농업적 및 사회·경제적 가뭄으로 분류된다. 그 중 강수량 부족으로 인해 야기되는 기상학적 가뭄은 지역 사회에 직접적인 피해를 유발하는 도화선이 된다. 그러나 강우량 부족이 경제적, 사회적 피해로 전파되는 과정은 단순하지 않으며, 정량화가 어려운 실정이다. 가뭄 위험도는 수문기상학적 요인뿐만 아니라 자연재해에 대한 지역의 취약성의 맥락에서 파악되어야하므로, 가뭄 위험도 평가 방안은 위험도와 지역사회 사이의 관계를 규명하는 과정을 포함해야 한다. 이러한 문제를 해결하기 위해 가뭄 취약성(vulnerability)과 노출성(hazard)을 정량화하여 나타내는 지표를 적용한 가뭄 위험도 분석 방법이 주로 사용된다. 일반적으로 수용되는 취약성 개념은 개인 또는 그룹이 자연재해의 영향에 대처하고 이에 저항할 수 있는 능력을 의미하며, 노출성은 자연적 또는 인간이 유발할 수 있는 물리적 사건의 가능성을 나타낸다. 따라서 취약성은 지역의 문화적, 사회적 및 경제적 인자를 이용하여 정의된다. 반면 노출성은 주어진 시간 또는 영역 내에서의 특정 현상의 발생 확률에 따라 결정된다. 본 연구에서는 가뭄 취약성 지수 (Drought Vulnerability Index, DVI)와 가뭄 노출성 지수(Drought Hazard Index, DHI)를 이용하여 지역 가뭄 위험도를 평가하였다. 취약성 및 노출성 지수를 활용한 선행연구에서의 주요 쟁점은 연구자의 주관성을 배제하고 지역의 상황을 반영할 수 있도록 (1) 객관적이고 합리적인 변수의 선택과 (2) 각 인자들의 관계를 규정하는 가중치 정의 방법이다. 본 연구에서는 확률론적 접근방법을 적용한 위험도 평가 방안을 제시하고자 하였다. DVI를 산정 시 지역에서 지배적인 사회경제적 인자를 선택하기 위해 주성분분석(PCA) 기법을 활용하였으며, DHI는 이변량 가뭄 빈도 분석에 의해 산정된 특정 가뭄사상의 발생 확률로 정의되었다. 본 연구에서는 국내에서 가뭄 위험도가 가장 높은 것으로 평가되는 충청북도 및 충청남도를 대상지역으로 선정하고 지역 위험도를 평가하였다. 그 결과 가장 가뭄 위험도가 높은 지역은 충청북도에서는 청주시, 충청남도에서는 공주시로 분석되었다. 특히 청주시는 DVI가 매우 높지만 DHI는 상대적으로 작게 나타났으며, 반면 공주시는 DHI와 DVI가 지역 내에서 가장 높게 산정되었다.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.159-163
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• 2013

The purpose of this study is to observe the growth habit and investigate a possibility of cultivating the GM rice (CaMsrB2-8) as a rice cultivar having drought resistance. Germination viability test showed that there was no significant difference between the drought-resistant GM(CaMsrB2-8) and non-GM (Ilmi) rice which was the parent variety at the GM rice. All the seeds of CaMsrB2-8 and Ilmi germinated after 6 days. Viviparous germination was not found in CaMsrB2-8 and Ilmi that was grown in greenhouse at $23{\pm}2^{\circ}C$ with water spraying for 40 days. Ratooning of CaMsrB2-8 and Ilmi was observed in 7-14 days and found uniform in field condition. CaMsrB2-8 seemed to grow faster than Ilmi. But CaMsrB2-8 and Ilmi were similar in 14-21 days. Both CaMsrB2-8 and Ilmi showed low seed shattering and more than 90% grains were ripened. All the seeds scattered in the paddy soil surface were not germinated after passing the winter. This study suggests that the drought-resistant GM rice was not significantly different with the parent variety of Ilmi in many agronomic characteristics such as wildness traits.

• Asian Journal of Turfgrass Science
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• v.12 no.3
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• pp.215-224
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• 1998

These experiments were conducted to compare the growth characteristics, cold and drought tolerances, disease tolerance and color of the 23 varieties of four different species of cool-season turfgrass introduced from the United States of America and Europe at Yonam College in 1995. Newport and Glade of 8 Kentucky bluegrass varieties were recorded better marks than others. Newport variety was recorded the good marks in the disease tolerance of summer seasons, uniformity, coverage and texture, but the bad marks in color, drought tolerance and rust tolerance and stay green of automn seasons. Glade variety was recorded the good marks in the disease tolerance of summer seasons, uniformity and coverage, but the bad marks in drought tolerance, texture and Pythium blight tolerance. Revel and Revel Jr of 5 tall fescue varieties were recorded better marks than others in color, shoot density, uniformity, coverage, and tolerance of Pythium and Fusarium blight disease. All of red fescue varieties died of disease for rainy season however texture was the finest of all the species in spring season. Dandy of 4 ryegrass varieties was recorded better marks than others in color, shoot density, coverage, drought tolerance and tolerance of Pythium and Fusarium blight disease but texture.

• Journal of Life Science
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• v.27 no.2
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• pp.194-201
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• 2017

Rapeseed (Brassica napus L.) is an oil crop classified as Brassicaceae, and it is widely grown worldwide. To develop a drought-resistant rapeseed, the ${\beta}$-glucosidase 1 (AtBG1) gene was introduced into rapeseed because drought- and salt-resistance phenotypes were observed when the AtBG1 gene was overexpressed in arabidopsis. Newly developed genetically modified crop must be proved to be safe. Safety assessments are based on the historical usage and scientific reports of a crop. In this study, we examined the potential acute oral toxicity of AtBG1 protein expressed in genetically modified (GM) rapeseed and calculated the minimum lethal dose at 6 weeks in both male and female ICR mice. AtBG1 protein was fed at a dose of 2,000 mg/kg body weight in five male and five female mice according to the marginal capacity concentration of OECD, 2,000 mg/15 ml/kg. Mortalities, clinical findings, and body weight changes were monitored for 14 days after dosing, and postmortem necropsy was performed on day 14. This study showed that no deaths occurred in the test group, and AtBG1 protein did not result in variations in common symptoms, body weight, and postmortem findings between the two groups. This showed that the minimum lethal dose of AtBG1 protein expressed in transgenic rapeseed exceed 2,000 mg/kg body weight in both sexes.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.4
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• pp.232-242
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• 2002

To investigate the effects of arbuscular mycorrhizal (AM) fungus (Glomus intraradices) colonization on drought-stress tolerance, leaf water potential, chlorophyll concentration, P content and carbohydrate composition were examined in perennial ryegrass (Lolium perenne L.) plants exposed to drought-stressed or well-watered conditions. Drought stress significantly decreased leaf water potential, P content and leaf growth. These drought-induced damages were moderated by mycorrhizal colonization. Drought stress decreased the concentration of soluble sugars in shoots. AM plants had a higher foliar soluble sugar than non-AM plants under drought stress condition. Drought stress depressed the accumulation of starch and fructan in shoots, but stimulated in roots. Under drought-stressed condition, starch concentration in roots was higher in non-AM plants than in AM plants. Fructan was the largest pool of carbohydrates, showing the highest initial concentration and the highest net increase for 28 days of treatment. Drought stress slightly decreased fructan concentration in shoots, but remarkably increased in roots. Under drought-stressed condition, fructan concentrations in non-AM and AM shoots at day 28 were 18.7% and 13.3% lower than the corresponding values measured at well-watered plants. However, in the roots, fructan accumulation caused by drought was lessen 13.6% by mycorrhizal colonization. The results obtained suggest that mycorrhizal colonization improves drought tolerance of the host plants by maintaining higher leaf water status and P status, and by retaining more foliar soluble sugars.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.5
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• pp.314-325
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• 2002

The effects of arbuscular mycorrhizal (AM) fungus (Glomus intraradices) on plant growth and N metabolic responses were examined in perennial ryegrass plants exposed to drought-stressed or well-watered condition. Mycorrhizal inoculation improved significantly leaf water potential, dry mass and P content. Drought stress increased significantly nitrate concentration in roots where the increase was much less in AM than non-AM. Drought stress decreased the concentration of soluble proteins in non-AM shoots, whereas non-significant decline occurred in AM shoots even under drought condition. The concentrations of ammonia and proline in drought stressed non-AM plants significantly increased, while mycorrhizal inoculation lowered significantly ammonia and proline accumulation. The decrease in leaf dry weight in drought stressed-plants was significantly correlated to the increase in ammonia (p<0.01) and proline concentration (p<0.01). These results suggested that the increased P content and N assimilation by mycorrhizal inoculation may be associated with drought stress tolerance, showing the moderating effects on shoot growth inhibition and ammonia accumulation in drought stressed-plants.

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.305-312
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• 2010

During a last decade, the introduced traits in commercialized GM crops have been diversified from a simple trait such as herbicide resistance gene or insectresistance gene which are related to the crop production into more complicated traits such as modification of fatty acid or essential amino acid composition, modified coloring pattern of flower. In addition, it was investigated that several other GM crops bearing more refined traits expected to lead next generation are also awaiting for risk assessment (RA) or under field test for the preparation of RA in the near future. These GM crops include abiotic stress resistance including drought or cold, increased biomass, production of bioethanol or diesel, production of pharmaceuticals or functional materials for industrial. In particular, in 2008 and 2009, it was reported that the highest number of GM crops for molecular farming are under developed in laboratory or green house level in all the world. Likewise, in Korea, 171 events from 49 plant species are under developed to introduce several important traits. At present, about 10 events are under field test to select elite lines for RA application. For the first time, herbicide resistance turfgrass developed by Korean research team has been submitted for RA and currently under requested for additional data. Moreover, GM rice resistant to leaf roll (folder) disease is expected as a next event to be submitted for RA application.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.24-24
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• 2017

홍수와 가뭄 등 수문기상재해 분석 및 사전 예측하기 위해서는 강수뿐만 아니라 토양수분, 증발산, 유량, 등과 같이 지표?하의 수문기상정보를 고려하는 것이 필요하다. 본 연구에서는 National Center for Atmospheric Research (NCAR)에서 개발된 고해상도 수문기상정보 모의가 가능한 WRF-Hydro를 활용하여 남강댐 유역에서 발생되는 돌발홍수 예측 적용성 평가를 수행하였다. 모델의 시공간 해상도는 1 hr, 150 m 이며, 기상 관측자료(Automatic Weather System, Automated Synoptic Observing System)를 사용하여 매개변수 민감도 실험을 실시하여 최적 모델 설정을 제시하였다. 고려된 매개변수는 격자 침투량을 결정하는 변수, 초기 저류 깊이, 표면 저항계수, 조도계수와 초기 토양수분 정보이며, 검증에 사용된 정보는 국가수자원관리종합정보시스템에서 1시간 단위로 제공되는 유입량 정보를 사용하였다. 그 결과 유출량은 격자 침투량을 결정하는 변수와 조도계수에 따라 민감하게 반응하였으며, 초기 토양수분량의 변화에 따라 시간에 따른 유출량의 변화가 강수에 민감하게 반응하는 것을 확인 할 수 있었다. 보정된 매개변수를 적용하여 돌발홍수 신고 지점의 유출량 변화를 살펴본 결과 강수의 발생과 동시에 매우 빠르게 유출량이 발생한 것을 볼 수 있었다.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.155-170
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• 2018

In this paper, mechanisms of gene editing technologies including ZFN, TALENS and CRISPR were briefly discussed with mutual advantages and disadvantages. Classification criteria of gene edited, site-directed mutagenesis (SDN) crops for regulatory purpose were also discussed. The number of studies using CRISPR technology was high and studies conducted on Arabidopsis thaliana and rice were highest, followed by tobacco, tomato, wheat, and corn. It has been applied to a variety of plants such as other grain crops, flower crops, vegetable crops, and fruit trees. The number of studies focused on practical application or commercialization in the future were also increasing yearly, and the scope of studies also expanded to include research on metabolic engineering for mass production of useful proteins or substances, development of disease resistant crops against viruses, bacteria, and fungi, abiotic environmental stressresistant crops, and increased yields. In addition to this, it was revealed that application range is becoming more diversified, including the development of parthenocarpic tomatoes, hybrid rice lines using male sterility and increased shattering resistance Brassica napus. It was also revealed that the number of CRISPR gene edited crops permitted by the USDA(APHIS) increases yearly, to be released in the international seed market soon.