• Journal of Food Hygiene and Safety
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• v.18 no.1
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• pp.6-13
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• 2003

Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.

• Journal of the korean Society of Automotive Engineers
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• v.16 no.1
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• pp.31-41
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• 1994

1. 가공성 평가실험 및 평가기술. 1.1 가공성 평가시험법. 1.2 실험방법. 1.3 피단한계. 1.4 파단발생 평가이론. 1.5 파단평가이론의 적용. Bulk재 소성가공 공정중에 있어서 거시적 가공성평가 방법 및 응용방법에 대하여 논하였다. Bolt Heading 가공과 같은 냉간압조 가공에 있어서 상기 모델들에 의한 재료고유의 가공한계선과 변형경로를 비교하므로써 가공성을 평가할 수 있으며 또한 보다 복잡한 형상제품의 최적가공을 위한 가공조건 및 재질선정의 최적화에도 유용하게 이용될 수 있다.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.9
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• pp.923-928
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• 2015

In this paper, we propose an ultrasonic magnetic abrasive polishing (US-MAP) technique to effectively machine a high-strength material, and we prove the efficiency of hybrid finishing. We use Taguchi's experimental method to determine the influence of each parameter. Based on the results, US-MAP exhibited a higher polishing efficiency than traditional MAP, and a suitable frequency for hybrid finishing was 28 kHz. When investigating the effect of the parameters on the surface roughness, the ultrasonic amplitude had the greatest effect. However, when machining with $55-{\mu}m$ amplitude, the machining efficiency decreased as the magnetic flux density varied.

• Food Engineering Progress
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• v.23 no.2
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• pp.125-133
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• 2019

The characteristics of extracts and precipitates after extraction at different water temperature (25, 50, 75, 95℃), ethanol ratio (25, 50, 75, 100%), and extraction method (stir, soak, autoclave) of yam powder and raw yam were investigated. The total polyphenol content was the highest in the 50% ethanol extract of yam powder. The DPPH radical scavenging activity was the highest in the 75% ethanol extraction and the crude saponin content was the highest in the 95℃ water extraction. Tyrosinase inhibitory activity was the highest in 95℃ water extraction, low concentration of ethanol extraction, and autoclave extraction. The peak viscosity, trough, and final viscosity of the precipitates increased after ethanol extraction, whereas decreased after the 95℃ water extraction and the autoclave, indicating the destruction of starch granules. This was confirmed by observing the starch granules broken using the SEM. The significance of this study was to investigate the possibility of the use of yam resources as a material, processing product development, skin beauty functional food and cosmetic material.

• The Korea Swine Journal
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• v.11 no.10 s.122
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• pp.124-126
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• 1989

• Food Industry
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• s.21
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• pp.18-22
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• 1974

• Journal of the KSME
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• v.40 no.12
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• pp.38-40
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• 2000

• The Journal of Korean Academy of Prosthodontics
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• v.58 no.1
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• pp.7-13
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• 2020

Purpose: This study was to evaluate marginal and internal discrepancy of 3-unit fixed dental prostheses (FDP) fabricated by subtractive manufacturing and additive manufacturing. Materials and methods: 3-unit bridge abutments without the maxillary left second premolar were prepared (reference model) and the reference model scan data was obtained using an intraoral scanner. 3-unit fixed dental prostheses were fabricated in the following three ways: Milled 3-unit FDP (MIL), digital light processing (DLP) 3D printed 3-unit FDP (D3P), stereolithography apparatus (SLA) 3D printed 3-unit FDP (S3P). To evaluate the marginal/internal discrepancy and precision of the prosthesis, scan data were superimposed by the triple-scan protocol and the combinations calculator, respectively. Quantitative and qualitative analysis was performed using root mean square (RMS) value and color difference map in 3D analysis program (Geomagic control X). Statistical analysis was performed using the Kruskal-Wallis test (α=.05), MannWhitney U test and Bonferroni correction (α=.05/3=.017). Results: The marginal discrepancy of S3P group was superior to MIL and D3P groups, and MIL and D3P groups were similar. The D3P and S3P groups showed better internal discrepancy than the MIL group, and there was no significant difference between the D3P and S3P groups. The precision was excellent in the order of MIL, S3P, and D3P groups. Conclusion: Within the limitation of this study, the 3-unit fixed dental prostheses fabricated by additive manufacturing showed better marginal and internal discrepancy than the those of fabricated by subtractive manufacturing, but the precision was poor.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.21-21
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• 2018

다양한 소재(금속, 세라믹, 고분자 소재 등)들이 3차원 형상기반 적층제조법에 적용되고 있는데, 금속 소재를 이용하여 3D 프린팅 법으로 치과용 수복물을 제조하는 연구가 많이 보고되고 있다. 하지만, 티타늄 또는 티타늄 합금 분말을 이용하여 3D 프린팅 법으로 제작한 치과용 보철물에 관한연구 보고는 많지 않다. Kanazawa 등 (2014)은 Ti-6Al-4V 합금분말을 이용하여 SLM법으로 총의치 용 framework를 제작하여 주조법으로 제작한 것과 비교 평가하였고, Mangano 등(2013)은 Ti-6Al-4V 합금분말로 지름이 작은 일체형 (1-piece narrow-diameter) 임플란트를 SLS법으로 제작하여 16명의 환자에게 식립한 다음, 2년간 관찰하였고, Mangano 등 (2014)은 cone-beam computed tomography (CBCT) data를 3D이미지로 변환시켜 DLMS법으로 치근 형상의 임플란트를 제작하여 15명의 환자에게 식립한 다음, 1년간 관찰하였다. 또한 서울대학교 및 연세대학교 치과생체재료과학교실 (2016)에서는 3D 프린팅 법으로 제작한 티타늄 시편과 기계 가공한 티타늄 시편의 물성을 비교하였다. 그러나 티타늄 합금 분말을 이용하여 3D 프린팅 법으로 제작한 치과용 보철물을 실제 임상에 적용하는 단계에서 기존 기계가공 방식으로 제작한 티타늄 보철물과 3D 프린팅 법으로 제작한 티타늄 보철물의 물성과 표면특성을 다양하게 비교 평가하는 것이 필요하여 본 연구에서는 3D 프린팅 법으로 제작한 티타늄 시편과 기계 가공한 티타늄 시편의 물성특성과 표면특성을 비교하여 조사하였다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1992.10a
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• pp.131-135
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• 1992

랩핑은 가공물의 치수정도와 다듬질면 정도의 향상을 위해 최종적으로 사용되는 가공법이다. 랩제(abrasive)로 서는 다이아몬드, SiC, 그리고 $Al_{2}$ $O_{3}$ 등이 사용되며 공작액과 혼합되어 가공물과 랩핑 블럭 사이에 끼이어 연삭작용을 하게 된다. 본 연구에서는 중요 가공변수등이 가공면 거칠기와 가공율에 미치는 영향을 실험측정하였고, 그 실험결과를 세라믹 랩핑가공의 기본적 연삭작용 기구인 취성파괴.현상을 기초로 하여 설명하였다.