• 제목/요약/키워드: }activity$

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당근(Daucus carota L.)배양세포의 DNase활성에 미치는 Polyamines의 영향 (The Effect of Polyamines on the DNase Activity in Cultured Carrot Cells)

  • 윤미정
    • Journal of Plant Biology
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    • 제29권4호
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    • pp.285-294
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    • 1986
  • The present study was attempted to investigate the effects of polyamines such as putrescine, spermidine and spermine on protein content and DNase activity in vivo and in vitro in carrot embryos. It was also investigated whether polyamines could replace role of cations required for DNase activity in vitro. The results obtained are as follows. Putrescine, spermidine and spermine increased protein content, although response to spermine reached plateau at the concentration of 0.1 mM. DNase activity was inhibited by polyamines, the inhibition being concentration-dependent and the highest att he concentration of 10 mM. The inhibition of DNase activity was the most prominent with spermine. Similar inhibitory effect to polyamines which was concentration-dependent was found in DNase activity but no change was shown on time-course in vitro. Putrescine and spermidine enhanced the DNase activity at low Mg2+ and Mn2+ concentrations, suggesting that the role of Mg2+ and Mn2+ for DNase activity could be, in part, replaced by these polyamines. These results, therefore, suggest that plyamines can modulate DNase activity through binding to DNA rather than direct effect on DNase activity.

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31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

  • Kim, Hyun-Won
    • Bulletin of the Korean Chemical Society
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    • 제32권1호
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    • pp.183-185
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    • 2011
  • Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

저장된 정보를 이용한 활동분석방법 연구 -ABC구축을 위한 활동분석 (A Study on the Activity Analysis Method using existing Saved Data -Activity Analysis for ABC Implementation)

  • 박주식;오지영;임총규;강경식
    • 대한안전경영과학회지
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    • 제3권2호
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    • pp.145-154
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    • 2001
  • This paper deals with Activity Analysis Method that is important procedure in Activity Based Costing System Implementation. There are many existing Activity Analysis Method, for example Interview, Questionnaire, Specialist Discussion and Work Measurement. Activity Analysis Data gained through this method has high reliability but this method bring about high cost. In case that certain company needs a strategic costing system, Activity Analysis Method which has high reliability will be need. But, if companies want the costing system as a internal decision making tool only, they need to design the ABC system fast and cheaply. This paper explains that Activity Analysis using existing finn material is good alternatives. So, this paper show the feasibility of Activity Analysis using existing firm material with comparing between job description, job specification information and Activity Analysis information.

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과학 놀이 활동이 아동들의 과학적 태도와 탐구능력에 미치는 효과 (An Effectiveness of Science-Play Activity on the Scientific Attitudes and the Scientific Inquiry Skills of Elementary School Students)

  • 김용권;이충형;이석희
    • 한국초등과학교육학회지:초등과학교육
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    • 제23권1호
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    • pp.17-26
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    • 2004
  • The purpose of this study is to verify the effect of science-play activity that isn't easily completed in a course of study, put in practice an development activity class on the scientific inquiry skills and the scientific attitude of elementary school students. For this purpose, the general activity was applied for the comparative group (40 students), the science-play activity was applied for the experimental group (34 students); they are composed of 5th and 6th graders elementary school students of 74, a class of an hour during a week. The results were analyzed by means of comparing between groups as well as within groups. The results of this research is as follows: Firstly, the science-play activity as an development activity was effective in the improvement of the scientific attitudes of elementary school students. Secondly, the science-play activity as an development activity was effective in the improvement of the scientific inquiry skills of elementary school students.

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Attenuated Sympathetic Activity and Its Relation to Obesity in MSG Injected and Sympathectomized Rats

  • Park, So-Young;Kim, Yong-Woon;Dan, Jin-Myoung;Kim, Jong-Yeon
    • The Korean Journal of Physiology and Pharmacology
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    • 제11권4호
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    • pp.155-161
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    • 2007
  • In order to characterize the role of sympathetic activity in obesity, we repeatedly assessed sympathetic activity via power spectral analyses of heart rate variability in the same subjects at 7, 11, 25, and 60 weeks, using monosodium glutamate (MSG)-induced obese and control rats. The effects of lower sympathetic activity on obesity were also evaluated. Fat mass in MSG rats was already higher at 7 weeks, but the sympathetic activity did not differ between 7 and 25 weeks. Between 25 and 60 weeks, the increase in fat mass, food efficiency, and body weight gain was higher in MSG rats. The increase in sympathetic activity between 25 and 60 weeks and sympathetic activity at 60 weeks were lower in MSG rats. Fat mass at 60 weeks was inversely correlated with changes in sympathetic activity between 25 and 60 weeks. Reduced plasma epinephrine levels by bilateral adrenal demedullation induced increase of fat mass. In, an attenuated increase of sympathetic activity with age may partly be responsible for aggravated obesity in MSG rats. Additionally, reduced sympathetic activity per se induced obesity in rats. These results suggest that lower sympathetic activity contributes to obesity in rats.

Ionospheric F2-Layer Semi-Annual Variation in Middle Latitude by Solar Activity

  • Park, Yoon-Kyung;Kwak, Young-Sil;Ahn, Byung-Ho;Park, Young-Deuk;Cho, Il-Hyun
    • Journal of Astronomy and Space Sciences
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    • 제27권4호
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    • pp.319-327
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    • 2010
  • We examine the ionospheric F2-layer electron density variation by solar activity in middle latitude by using foF2 observed at the Kokubunji ionosonde station in Japan for the period from 1997 to 2008. The semi-annual variation of foF2 shows obviously in high solar activity (2000-2002) than low solar activity (2006-2008). It seems that variation of geomagnetic activity by solar activity influences on the semi-annual variation of the ionospheric F2-layer electron density. According to the Lomb-Scargle periodogram analysis of foF2 and Ap index, interplanetary magnetic field (IMF) Bs (IMF Bz <0) component, solar wind speed, solar wind number density and flow pressure which influence the geomagnetic activity, we examine how the geomagnetic activity affects the ionospheric F2-layer electron density variation. We find that the semi-annual variation of daily foF2, Ap index and IMF Bs appear clearly during the high solar activity. It suggests that the semi-annual variation of geomagnetic activity, caused by Russell-McPherron effect, contributes greatly to the ionospheric F2-layer semi-annual electron density variation, except dynamical effects in the thermosphere.

Biological activity of an Indian medical plant, Indigofera cordifolia

  • Rao, Bhattiprolu Kesava;Kawase, Masami;Tanaka, Toru;Tani, Satoru;Motohashi, Noboru;Satoh, Kazue;Sakagami, Hiroshi;Terakubo, Shigemi;Nakashima, Hideki;Wolfard, Krisztina;Molnar, Joseph
    • Advances in Traditional Medicine
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    • 제4권3호
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    • pp.179-185
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    • 2004
  • The ethanol extract of Indigofera cordifolia was studied for in vivo gastroprotective activity, cytotoxic activity against oral tumor and normal cells, multidrug resistance (MDR) reversal activity, anti-human immunodeficiency virus (HIV) activity and radical scavenging activity. The extract of I. cordifolia showed potent gastric mucosal protective activity against stomach injury induced by HCl/EtOH solution. However, the gastroprotective activity could not be related to the radical mechanism, because the extract weakly scavenged both OH radical and $O_2*^-$. The extract also showed promising levels of MDR-reversing activity. This study demonstrates the tumor-specific cytotoxic action of the plant extract. However, the extract had no anti-HIV activity. From above results, the study suggests the medicinal importance of I. cordiforia extract.

경로당 여성노인의 신체활동에 대한 영향요인 (Factors Influencing Physical Activity in Elderly Women at Senior Citizen Centers)

  • 서경희;은영
    • 근관절건강학회지
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    • 제22권3호
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    • pp.236-244
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    • 2015
  • Purpose: The purpose of this study was to investigate physical activity of elderly women at senior citizen centers and to identify factors influencing physical activity. Methods: The study was conducted from February 22 to April 20, 2013 for 200 elderly women at senior citizen centers in J City. Levels of physical activity, cognitive function, and nutritional status were measured. Results: The total physical activity amount was $2118.94{\pm}2.36$ MET-min. The average cognitive function score was $21.17{\pm}4.63$, and the average nutritional status score was $24.04{\pm}3.37$. There were significant relationships among physical activity, cognitive function, and nutritional status. In addition, there were significant differences of groups of physical activity by levels of cognitive function and nutritional status. Cognitive function, nutritional status, and age were significantly associated with level of physical activity in elderly women. Conclusion: The results showed many older women were doing moderate physical activity. Physical activity was associated with cognitive function and nutritive conditions. Integrated health promotion program needs to be implemented to increase physical activity level, the cognitive function, and nutritive conditions in elderly women.

일부 농촌주민 혈중 Paraoxonase 활성도의 분포 및 관련인자에 관한 조사연구 (A Study on the Distributions of Paraoxonase Activity and the Factors affecting Paraoxonase Activity of a Rural Population)

  • 송재철;박항배
    • Journal of Preventive Medicine and Public Health
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    • 제23권2호
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    • pp.194-200
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    • 1990
  • The paraoxonase (E. C. 3.1.1.2) is a major enzyme to detoxicate the organophosphorus and carbamate which are the most widely used as the agricultural spraying insecticides. To investgate the distributions of plasma paraoxonase activity and the factors affecting the enzyme activity, the plasmas of 945 Korean rural population were analysed with the modified Krisch's direct sphectrphotometry method. Three indices of the enzyme activity - basal activity, stimulated activity (by NaCl), % stimulation - were obtained from the analysis. Three indicies suggested unimodal distributions, so we couldn't identify the low activity group risk group to organophosphorus & carbamate insecticides poisoning. There is no significant relation between 3 actvity indicies and sex, age, or history of insecticide use (p>0.05). The basal activity and the stimulated activity have significant relationship and high coefficient of determination with the activities of their parents ($r^2$=0.30, 0.24 ; p<0.05), but the % stimulation does not ($r^2$=0.02 ; p<0.05). These results suggest that the activity of paraoxonase is determined mainly by the genetic factor.

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PDCA 모형에 기초한 QI활동 평가틀 개발 및 사례분석 (Development of QI Activity Evaluation Framework Based on PDCA and Case Study on Quality Improvement Activities)

  • 박연화;이명하;정석희
    • 간호행정학회지
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    • 제18권2호
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    • pp.222-233
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    • 2012
  • Purpose: This study was conducted to develop an evaluation framework for QI activity in medical institutions and to analyze QI activity cases by applying the developed evaluation framework. Method: A four-phase process was employed to develop the evaluation framework, and a descriptive survey was used for the QI case study. Data were collected in April, 2010 by examining 157 QI activity cases presented at conferences and published in Journal of Korean Society of Quality Assurance in Health Care over the past three years. Developed QI activity evaluation instruments were used for data collection. Data were analyzed using the SPSS 18.0 for Windows program. Result: A QI Activity Evaluation Framework was developed. This framework consisted of 45 items. The department with the highest level of QI participation was the nursing department. The most frequent QI activity theme was patient safety. QI activity levels in Korean medical institutions are relatively equalized without significant differences according to institution characteristics. Conclusions: From the quality aspect of QI activity, more systematic and scientific approaches are required to upgrade QI activity. This study could provide methodological guidelines for QI activity and be useful in setting goals and directions for QI activity in medical institutions in Korea.