• 대한수학회논문집
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• 제14권2호
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• pp.373-383
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• 1999

The ($\alpha$,$\beta$)-metric is a Finsler metric which is constructed from a Riemannian metric $\alpha$ and a differential 1-from $\beta$;it has been sometimes treated in theoretical physics. The condition for a Finsler space with an ($\alpha$,$\beta$)-metric L($\alpha$,$\beta$) to be projectively flat was given by Matsumoto [11]. The present paper is devoted to studying the condition for a Finsler space with L=$\alpha$\ulcorner$\beta$\ulcorner or L=$\alpha$+$\beta$\ulcorner/$\alpha$ to be projectively flat on the basis of Matsumoto`s results.

• KSBB Journal
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• 제6권1호
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• pp.9-14
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• 1991

We studied the production and excretion of alpha-interferon in recombinant Escherichia coli harboring plasmid pIF-III-B, which carries alpha-interferon gene under the control of lipoprotein and lacUV5 promoter, and lac operator. Basically, the effects of concentrations of ampicillin and an inducer, IPTG, for the expression of the cloned gene, on the productions of alpha-interferon and plasmid stability were studied. The highest production of alpha-interferon was observed at 50 mg/1 of ampicillin concentration and 0.5 mM of IPTG. The plasmid pIF-III-B was maintained very stably in medium with ampicillin but segregated rapidly in medium without ampicillin. Also, the plasmid was segregated more rapidly in medium with an inducer higher than 0.5 mM.

• KSBB Journal
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• 제5권3호
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• pp.293-298
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• 1990

대장균의 lipoprotein promoter, lac UV5 promoter 및 operator와 lipoprotein의 signal sequence를 가지는 vector에 alpha-IFN유전자를 cloning함으로써 제작된 plasmid plF-III-B와 plF-III-C를 여러종류의 대장균 숙주 세포에 형질 전환하여 IFN의 생산성을 조사해 본 결과 $lpp^-$형질을 가지는 JE5505가 가장 우수했으며, 기존 plasmid, Hif-2h에 비해 130배 높은 생산성을 보였다. 또한 생산된 IFN의 약 50%가 세포 외부로 분비됨을 확인하였다.

• KSBB Journal
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• 제5권3호
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• pp.195-200
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• 1990

대장균의 lipoprotein promoter, lactose promotor 및 operator 와 lipoprotein의 signal sequence 를 가지는 vector에alpha-IFN 유전자가 cloning된 plasmid pIF-Ill-B를 여러종류의 대장균 숙주 세포에 형칠전환하여 alpha-IFN 의 생산성, 생장특성을 조사하였다. 또한 plasmid 자체와 cloning된 alpha-IFN 유전자의 발현유도가 세포생장에 미치는 영 향을 조사하였다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 1998년도 제4차 학술발표대회 및 정기총회
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• pp.59-60
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• 1998

A DNA construct containing rat T $\alpha$ 1 $\alpha$ -tuulin gene 5'-flanking sequence and GFP reporer gene was microinjected into 1-cell loach embryos. Neuron-specific FGP expression was observed in developing loach embryos and early stage fry. The results demonstrated that rat T $\alpha$ 1 $\alpha$ -tubulin gene promoter may be sufficient to specify gene expression to neurons in loach embryos. Thus, the use of GFP reporter controlled by T $\alpha$ 1 $\alpha$ -tubulin gene promoter may facilitate visualization of the dynamic processes of neural tissue development.

• 약학회지
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• 제41권5호
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• pp.638-646
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• 1997

The steroid 9${alpha}$-hydroxylase activity has been detected in cytosol fraction, $100,00{\times}g$ supernatant of cell free extract of Mycobacterium fortuitum. The activity was not linear with protein concentration in the assay suggesting 9${alpha}$-hydroxylase is a multicomponent enzyme. The 9${alpha}$-hydroxylase system was partially purified through fractional saturation of ammonium sulfate, strong anion exchange (Mono Q) column chromatography, gel filtration (Superose 12) column chromatography, and testosterone affinity gel chromatography. Ammonium sulfate 50~60% saturated fraction of the cytosol gave 9${alpha}$-hydroxylase activity. For further purification, the half-saturated ammonium sulfate fraction was applied to Mono Q, Superose 12, or affinity gel column. The purification factors of 9${alpha}$-hydroxylase containing fraction after Mono Q, Superose 12, and affinity gel chromatography was 13, 11, and 17 respectively.

• 한국연초학회지
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• 제24권2호
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• pp.94-100
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• 2002

Cyclodextrin glucanotransferase from Bacillus stearothemophilus and Bacillus macerans synthesized maltol and ethyl maltol monoglucoside, with a series of its maltooligo-glucosides by transglycosylation with dextrin as a donor, and maltol or ethyl maltol as an acceptor. The monoglucoside formed from reaction mixture of maltol or ethyl maltol by the successive actions of Bacillus stearothemophilus cyclodextrin glucanotransferase and Rhizopus glucoamylase was isolated by Diaion HP-20 column and silica gel column chromatography. The structure of the isolated monoglucoside was identified as maltol-$\alpha$-D-glucoside and ethyl maltol-$\alpha$-D-glucoside, respectively, by FAB-MS, UV, $^1$H-NMR, $^{13}$ C-NMR spectra and products by hydrolysis with acid, $\alpha$ - and $\beta$ -glucosidases.

• 미생물학회지
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• 제38권2호
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• pp.139-143
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• 2002

탄수화물분해 효소인 $\alpha$-glucosidase의 활성을 저해하는 물질을 생성하는 미생물을 검색하기 위하여 토양시료로 부터 수종의 미생물을 검색하였다. 토양 시료에서 강력한 $\alpha$-glucosidase 저해제를 생성하는 방선균PM718을 분리하였고 그 저해제의 활성을 기지물질인 acarbose와 비교 측정한 결과 우수한 활성을 나타냄을 확인하였다. 또한 그 활성은 pH 변화에도 안정된 활성을 유지하는 것을 확인하였다.

• East Asian mathematical journal
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• 제21권2호
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• pp.233-240
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• 2005

Let $CS^{\alpha}(\beta)$ denote the class of normalized strongly $\alpha$-logarithmic close-to-convex functions of order $\beta$, defined in the open unit disk $\mathbb{U}$ by $$\|arg\{\(\frac{f(z)}{g(z)}\)^{1-\alpha}\(\frac{zf'(z)}{g(z)\)^{\alpha}\}\|\leq\frac{\pi}{2}\beta,\;(\alpha,\beta\geq0)$$ where $g{\in}S^*$ the class of normalized starlike functions. In this paper, we prove sharp $Fekete-Szeg\"{o}$ inequalities for functions $f{\in}CS^{\alpha}(\beta)$.

• 대한화학회지
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• 제18권5호
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• pp.368-372
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• 1974

Bombina Orientalis(무당개구리)의 복피로부터 추출한 암적색의 색소를 TLC, PLC(preparative thin layer chromatography) 및 column chromatography로 분리 및 정제하여 물리, 화학적 성질 visible 및 IR-spectral 특성을 이용하여 세번째주색소인 ${\alpha}$-cryptoxanthin(3-hydroxy-${\alpha}$-carotene)을 확인하였다