• Asian-Australasian Journal of Animal Sciences
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• v.14 no.7
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• pp.979-985
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• 2001

Two experiments on commercial broiler chickens (3-37 d) and WL layers (252-364 d) were conducted to study the relative bioavailability of phosphorus (P) from different P supplements in comparison to tricalcium phosphate (TCP), at constant dietary calcium (Ca):P ratio. The P sources tested were dicalcium phosphate (DCP), steam sterilized bone meal (SSBM), commercial mineral mixture (CMM), phosphoric acid (PA) and a combination of CMM + PA. Fluorine (F) content in CMM and SSBM was 13.12 and 0.14 g/kg, respectively. In commercial broiler diets, DCP, SSBM or PA could be used as supplemental P sources without affecting (p<0.05) weight gain, feed intake, tibia ash and, P and Ca contents in tibia ash when compared to TCP. Severity of leg abnormality and deposition of F in bone were higher (p<0.05) in group fed CMM. P retention and serum inorganic P content was significantly reduced (p<0.05) in CMM fed birds compared to those fed TCP, SSBM, PA or CMM+PA. Among other P sources (TCP, SSBM, PA and CMM+PA) the serum inorganic P levels did not vary significantly (p<0.05). The P retention also significantly reduced in CMM fed groups compared to those fed DCP or PA. The P retention significantly increased from 0.183 to 0.216 units by supplementation of PA to CMM diet. In layers, egg production was not affected by replacing TCP with DCP, SSBM, PA or CMM+PA, but significantly (p<0.05) reduced with CMM. Feed (kg)/kg egg mass, egg weight, shell quality (shell weight and shell thickness) and serum Ca levels were not influenced by dietary variation in P source. The poor performance of both broilers and layers fed on CMM based diets could be attributed to the presence of hlgher levels of F (647.8 and 630.1 mg/kg, respectively) and low P utilization. Based on growth, bone mineralization and P retention it is concluded that DCP, SSBM or PA can be used as alternatives to TCP in broiler diets. In WL layer diets, in addition to above P sources, CMM can also be used as supplemental P source by replacing one half of P from CMM with PA without affecting egg production and shell quality.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7737-7741
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• 2013

Background: Human papilloma virus infection is associated with genesis and malignant potential of cervical cancer. E6 and E7 oncogens are known to bind to p53 and retinoblastoma gene products, abrogating their functions as tumor suppressors, leading to an abnormal cell cycle machinery. Roles of the p53 homolog p63 have also been postulated, E6 expression leading to TAp63b degradation allowing anchorage independent growth. Molecular studies correlated with clinicopathological factors are important to determine prognosis and treatment strategies, but results have been controversial and need to be clarified. Aim: To investigate expression of p53 and p63 in cervical squamous cell carcinomas in correlation with age, FIGO staging, morphology, and cancer cell proliferation. Materials and Methods: Expression of p53 and p63 immunohistochemical staining in a total of 56 paraffin-embedded tissues of cervical squamous cell carcinomas from Dr. Sardjito General Hospital Indonesia, was evaluated for correlation with clinicopathological parameters. The Mann-Whitney test was used to compare the percentage of p53 and p63 expression with patient age, FIGO staging and morphology and to compare mean p53 and p63 expression. The Spearman correlation test was applied to correlate p53 and p63 expression with that of Ki-67. A p-value of <0.05 was considered statistically significant. Results: There were significant associations between p53 expression with age (p=0.019) and FIGO staging (p=0.026), but not with with morphology or Ki-67 expression. There were no links between p63 expression and age, morphology, FIGO staging or Ki-67. Conclusions: This study indicated that p53 has a prognostic value in cervical squamous cell carcinomas given the relation with FIGO staging.

• The Journal of the Korea Contents Association
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• v.17 no.1
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• pp.662-672
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• 2017

Recently, with the development of mobile devices and near field communication, mobile P2P networks have been actively studied to improve the limits of the existing centralized processing system. A peer has limited components such as batteries, memory and storage spaces in mobile P2P networks. The trust of a peer should be discriminated in order to share reliable contents in mobile P2P networks. In this paper, we propose a trust discrimination scheme considering limited resources in mobile P2P environments. The proposed scheme discriminates the trust of a peer by direct rating values using the rating information of the peer and indirect rating values by the other peers. The recent update time is included in the rating information. The proposed scheme reduces the redundant rating information by comparing the recent update times of the rating information. It is shown through performance evaluation that the proposed scheme reduces the number of messages and improves the accuracy of trust over the existing scheme.

• Polymer(Korea)
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• v.33 no.5
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• pp.441-445
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• 2009

In order to develop an intelligent delivery system for cosmetic active agents (e.g., arbutin, ascorbic acid, and adenosine), pH-responsive P(MAA-co-PEGMA) hydrogel particles having an average size of approx. $2{\mu}m$ and spherical shape were synthesized via dispersion photopolymerization. There was a drastic change in the swelling ratio of P (MAA-co-PEGMA) hydro gels at a pH 5, which is the $pK_a$ of the hydrogel. To determine the factors that have an effect on the loading of cosmetic active agents into the hydrogel, the loading efficiency of the active agents was investigated at various MAA and EG compositions of the hydrogel and the loading pH conditions. When the MAA contents in the hydrogel decreased, the loading efficiency of the active agents increased. In loading experiments with different pH conditions, the active agents showed higher loading efficiency into the hydrogel at the pH where the hydrogel was at the swollen state than at the pH where the electrostatic repulsion was minimized.

• Research in Community and Public Health Nursing
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• v.1 no.1
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• pp.503-517
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• 1989

This study is to analyze factors affecting the school nurse's activities. The survey was undertaken during Sept. 1-Nov. 30, 1986. The subjects were 137 school nurses from elementary, junior-high, and senior-high schools in Daegu City and Kyungppk Province. The results are as follows: 1. Correlational findings between school nurse's self-confidence and their general characteristics 1) Program Planning & Evaluation: Health Experinece(r=-0.1803, p<0.05) Salary Step(r=-0.1741, p<0.05) 2) Clinic Management: Salary STep(r=-0.2580, p<0.01) 3) Health Education: Salary Step(r=-0.1929, p<0.05) 4) Management of School Environment: Salary Step(r=-0.2501, p<.05) 5) Health Care Services: Health Experience(r=0.1901, p<0.05) Salary Step(r=-0.2424, p<0.05) 2. The degrees of school nurse's self-confidence(high: 4 point, low: 1 point) 1) Clinic Management: 2.92 2) Health Education: 2.86. 3) Program Planning & Evaluation: 2.74 4) Health Care Services: 2.73 5) Management of School Environment: 2.67 6) Operating of School Health Organization: 2.42 3. Significances to self-confidence on school nurse's activities 1) Program Planning as Evaluation: Expending Times for Health Care Services (r=-0.2262, p<0.05) Expending Times for Health Education (r=0.2943, p<0.05) Size of Clinic(r=0.2163, p<0.05) Location of Clinic(t=2.43, gH0.047) Use of Clinic(t=2.06, p<0.007) 2) Clinic Management: Location of Clinic (t=3.36, p<0.010) 3) Health Education: Purchase of Medicine(r=-0.1736, p<0.05) No, of Classes (r=-0.1794, p<0.05) (4) Management of School Environment: School Health Budget(r=0.1731, p<0.05) Home Message(r=0.1805, p<0.05) Location of Clinic(t=4.46, p<0.0001) 5) Operating of School Health Organization: School Health Budget(r=0.1878, p<0.05) Use of Clinic(t:1.90, p<0.018) 6) Health Care Services: School Health Budget(r=1.90, p<0.018) Expending Times for Health Education(r=0.2577, p<0.05) Size of Clinic(r=0.4336, p<0.001) Location of Clinic(t:5.10, p<0.001)

• Journal of Korean Public Health Nursing
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• v.10 no.1
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• pp.80-94
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• 1996

This study was to investigated the complaints of physical and mental health problem of professors and officials in H University of Seoul and collected during the period from April 25 to May 31. 1995. The complaints of physical and mental health problem were measured by Cornell Medical Index. The results of this study are as follows: 1. Among the items of physical health problem. respondents showed the highest rate of complaints related to fatigability. and among the items of mental health problem. respondents showed the highest rate of complaints related to inadequacy. 2. Females showed significantly higher rates of complaints related to the musculoskeletal system(p<0.001), fatigability(p<.001). habits(p<.01), inadequacy(p<.05). and tension(p<.001) compared with those of males. Twenties showed significantly higher rates of complaints related to the digestive system(p<.01) compared with those of other age groups. Singles showed significantly higher rates of complaints related to the digestive system(p<.05). inadequacy(p<.01) and depression(p<.001) compared with those of marries. Officals showed significantly higher rates of complaints related to the cardiovascular system(p<.01). digestive system(p<.05), musculoskeletal system(p<.05), and tension(p<. 05) compared with those of professors. Resondents who have irregular eating habits showed significantly higher rates of complaints related to the digestive system(p<.01), musculoskeletal system(p<.05). habits(p<.05). and depression(p<.001) compared with those of respondents who have regular eating habits. Respondents who usually sleep below 6 hours a day showed significantly higher rates of complaints related to the cardiovascular system(p<.01). digestive system(p<.05). musculoskeletal system(p<.01). fatigability(p<.05). habits(p<.01). and tension(p<.05) compared with those of respondents who sleep above 6 hours.

• Journal of Gastric Cancer
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• v.3 no.4
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• pp.206-213
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• 2003

Purpose: The clinical implication of p53 mutation in gastric cancer is still unclear, as shown by the discordant results that continue to be reported in the literature. Materials and Methods: To assess p53 gene mutation, tumor p53 overexpression, and serum anti-p53 antibody, we employed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, an immunohistochemistry using monoclonal antibody DO-7, and an enzymelinked immunosorbent assay (ELISA), respectively. Results: Of 169 surgical specimens of gastric cancer, mutation at exon $5\∼8$ of the p53 was identified in 33 ($19.5\%$) and was significantly correlated with lymph node metastasis. Overexpression of p53 was found in 62 specimens ($36.7\%$) and had a significant correlation with tumor differentiation. Serum anti-p53 antibody was positive in 18 patients ($10.7\%$). Twenty-three of the mutated tumors ($69.7\%$) and 39 of the non-mutated tumors ($28.7\%$) displayed immunoreactivity. Twelve of the immunopositive tumors ($19.4\%$) and 6 of the immunonegative tumors produced anti-p53 antibody. These differences were statistically significant (P<0.001 and P=0.005, respectively). There was no significant difference in survival according to the mutation of p53. Conclusion: Mutation and overexpression of p53 can be easily detected by immunohistochemistry. However, standardization of the immunohistochemical staining method, as well as guidelines for interpreting the stained result, will produce concordant results and thereby improve clinical application.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.20-27
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• 1997

Objectives of this study was to investigate effects of supplemental chromium (Cr) as CrP in growing pigs treated with pST. Seventy two Landrace pigs weighing average 60 kg were alloted to the three treatments during the 52-d experimental period: control (corn-soybean basal diet); pST treatment (4 mg/head/day); pST + CrP treatment (4 mg and 200 ppb/head/day). Upon termination of feeding trial weighing average 105 kg, thirty-six pigs randomly selected from each treatment were slaughtered to compare carcass traits. For the study of lipid metabolism, eighteen pigs were alloted to the same treatments. Adipose tissue samples from eighteen pigs were collected to investigate lipid metabolism. All treated samples with pST and pST + CrP showed improvements in daily weight gain, regardless of sex. Feed/gain ratio significantly improved in pigs treated with pST and pST + CrP. Dressing percentages were higher in pigs treated with pST and pST + Crp. Carcass grades were significantly higher in pigs treated with pST and pST + CrP. Lipolysis of adipose tissue measured in vitro was significantly increased in pigs treated with pST, lipogenesis in vitro showed opposite tendency. Even though the current data does not show synergistic effects on the above parameters when CrP and pST were supplied at the same time, but CrP supplementation tended to improve growth performance and carcass traits of pigs treated with pST.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.671-676
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• 2005

In this study, we investigated the antioxidant effect of extract from Monascus pillosus, on the human wild-type p53 and p21 expressing A549 lung epithelial cell line and MCF-7 mammary adenocarcinoma cell line stimulated by NO. $P21^{waf/cip1}$ was identified as a gene induced in senescent cells. It is a cyclin-dependent kinase inhibitor and has been shown to cause cell cycle arrest and apoptosis. While p53-regulated stimulation of p21 appears to be central for the permanent growth-arrest, the role of p21 in p53-triggered cell death is unclear. Low dose of sodium nitroprusside (SNP) induced the development of senescence associated with increased expression of p53 and p21 in A549 cells. Inhibition of p21 transactivating activity requires high level correlates with the amount of p53 necessary to cause cell death. Association of p21 and p53 results in inhibition of p21-stimulated transcription. This requires a higher p53 level than is necessary for transcriptional activation of endogenous p53-responsive gene but correlates well with the level of p53 necessary to cause cell death. Exposure to W-1 inhibited oxidative stresses-induced senescence-like arrest, resulting in a significant reduction in p53 and p21 steady state levels. These results suggest that p53 and p21 play a central role in the onset of senescence. Thus, it is important to emphasize control of oxidative balance in tumor prevention and aging.

• Animal cells and systems
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• v.13 no.4
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• pp.399-403
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• 2009

$p19^{ras}$ is an alternative splicing variant of the proto-oncogene c-H-ras pre-mRNA of $p21^{ras}$. In contrast to $p21^{ras}$, $p19^{ras}$ does not have a C-terminal CAAX motif that targets the plasma membrane and is localized to both the cytoplasm and nucleus. We found that $p19^{ras}$ activated the transcriptional activity of $p73{\beta}$ through protein-protein interactions in the nucleus. p73 is known to play an important role in cellular damage responses such as apoptosis. Although p73 is a structural and functional homologue of p53, p73-mediated apoptosis has not yet been clearly elucidated. In this study, we demonstrate that the interaction between $p19^{ras}$ and $p73{\beta}$ accelerated $p73{\beta}$-induced apoptosis through a caspase-3 dependent pathway. Treatment with DEVD-CHO, a caspase inhibitor, also strengthened $p73{\beta}$-mediated apoptosis through a caspase-3 dependent pathway. Furthermore, the enhanced transcriptional activity of endogenous $p73{\beta}$ by treatment with Taxol was amplified by $p19^{ras}$ overexpression, which markedly increased caspase-3 dependent apoptosis in the p53-null SAOS2 cancer cell line. Our findings indicate a functional linkage between $p19^{ras}$ and p73 in caspase-3 mediated apoptosis of cancer cells.