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Evaluation of Antimicrobial Activities of Rhubarb Extracts on Putrefactive Microorganisms Related to Soybean Curd (Doobu) (두부 부패 미생물에 대한 대황(Rhubarb) 추출물의 항균 활성 평가)

  • Kim, Chul-Jai;Suh, Hee-Ji;Chung, Hee-Sook
    • Journal of the Korean Society of Food Culture
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    • v.21 no.2
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    • pp.225-231
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    • 2006
  • 대황(Rhubarb)은 중국과 일본에서 혈액 순환제, 진통제, 신장치료제 등으로 전통적으로 사용되고 있는 약용식물이다. 이 연구에서는 한국산 R. undulatum L.의 뿌리 추출물, 미국산 R. rhabarbarum L.과 중국산 R. palmatum L.의 줄기 추출물의 두부 부패에 관여하는 미생물에 대한 항균 활성을 평가하였다. Rhubarb의 최적 추출을 위해서 각각 50%, 70%, 80% 에탄올을 용매로 사용하였으며, 항균 활성은 Kirby-Bauert test, minimum inhibitory concentration (MIC)과 minimum bactericide concentration (MBC)에 의해서 평가되었다. Kirby-Bauert test 결과, R. undulatum L.의 뿌리 추출물은 대부분 두부 부패균에 대해서 항균 활성을 가지는 것으로 나타났으며, R. rhabarbarum L.의 줄기 추출물의 경우는 $20{\mu}g/disc$의 농도에서는 항균 활성이 거의 나타나질 않았으나, 고농도로 갈수록 높은 항균 활성을 나타내었다. 또한 R. palmatum L.의 줄기 추출물은 고농도로 갈수록 항균 활성이 높아졌으나, Pseudomonas aeruginosa에 관해서는 항균 활성이 나타나지 않았다. MIC와 MBC에 의한 항균 활성 평가실험에서는, 세 종류의 rhubarb 추출물 중에서 phenolic 화합물이 가장 많이 함유되어 있는 R. undulatum L.의 뿌리 추출물보다 R. rhabarbarum L.의 줄기 추출물이 MIC와 MBC 값이 낮았다. 이는 phenolic 화합물의 양 이외에 낮은 pH가 항균 활성에 영향을 준 것으로 판단된다. 본 연구를 통해서 rhubarb 추출물은 두부 부패에 관여하는 미생물에 대해서 항균 활성을 가지는 것으로 나타났으며, 천연 항균제로써 식품에 적용 가능성이 있는 것으로 평가되었다. 87.1%이었으며, 아침을 매일 먹는 채식남학생은 78.8%, 비채식남학생은 33.3%, 채식여학생 47.1%, 비채식여학생 39.6%이었다. 또한 식사의 양은 과식한다는 응답이 채식남학생 24.2%, 비채식남학생 38.1%, 채식여학생은 29.4%, 비채식여학생 40.6%으로 비책식군의 과식율이 높았다. 5. 식품 섭취빈도는 두부 및 콩제품을 매일 섭취하는 경우는 채식남학생 54.6%, 비채식남학생 16.7%, 채식여학생은 38.2%, 비채식여학생이 16.8%이었다. 우유 및 유제품을 매일 섭취하는 경우는 채식남학생 6.1%, 비채식남학생 33.3%, 채식여학생 14.7%, 비채식여학생은 21.8%이었으며, 녹차, 커피 등 차를 마시지 않는다는 비율은 채식남학생 69.7%, 비채식남학생 28.6%, 채식여학생 29.4%, 비채식여학생 25.7%이었다. 인스턴트 식품을 매일 섭취한다는 응답율이 채식남학생 9.1%, 비채식남학생 21.4%, 채식여학생은 17.7%, 비채식여학생은 14.9%이었다. 6. 운동, 체중 조절 등에 대한 조사 결과 항상 운동을 하는 경우는 채식남학생 30.3%, 비채식남학생 28.6%, 채식여학생 14.7%, 비채식여학생 18.8%이었으며 운동시간은 $1{\sim}2$시간 하는 경우는 채식남학생 30.3%, 비채식남학생 38.1%, 채식여학생은 8.8%, 비채식여학생은 17.8%이었다. 체중에 만족하는 정도를 보면 채식남학생 57.6%, 비채식남학생 23.8%, 채식여학생은 23.5%, 비채식여학생은 15.8%가 만족한다고 하였다. 체중 조절 경험에서 경험이 있는 경우가 채식남학생 3.0%, 비채식남학생 31.0%, 채식여학생은 23.5%, 비채식여학생 31.7%이었다. 7. 골밀도 BQI값과와 몇가지 요인의 상관관계를 살펴보았을때, 채식남학생은 영양보충제의 섭취와 유의적인 양의 상관관계를, 해조류의 섭취정도와 유의적인 음의 상관관계를 나타내었다.

Fish Community Characteristics, and Habitat Characteristics and the Age of the Eight Barbel Loach, Lefua costata (Pisces: Namacheilidae) in the Jusucheon of Gangneung-si (강릉시 주수천의 어류군집 특징 및 쌀미꾸리 Lefua costata (Pisces: Namacheilidae)의 서식지 특징과 연령)

  • Han, Mee-Sook;Ko, MyeongHun
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.160-171
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    • 2022
  • This study investigated the characteristics of fish communities, habitat characteristics and the age of Lefua costata in the Jusucheon of Gangneung-si, Korea from January to December 2013. The survey collected 23 species belonging to ten families from 6 survey stations. The dominant and subdominant species were Zacco koreanus (relative abundance, 22.1%) and Rhynchocypris steindachneri (20.1%), respectively. The next most abundant species were Tridentiger brevispinis (10.7%), Orthrias nudus (9.7%), Zacco platypus (8.6%), Lefua costata (7.9%), Iksookimia koreensis (6.0%) and Pungitius sinensis (3.0%). Among the fish species collected, one species, P. sinensis, was class II endangered wildlife by the Ministry of Environment, and four species (Z. koreanus, I. koreensis, Silurus microdorsalis and Cottus koreanus), were endemic to Korea. Additionally, five migration fish species (Tribolodon hakonensis, Hypomesus nipponensis, Gasterosteus aculeatus, Oncorhynchus keta and Plecoglossus altivelis) were collected. The similarity index based on species composition and numbers separated fish communites in the Jusucheon according to main section, i.e., uppermost(St. 1), upper (St. 2~4), middle (St. 5), lower (St. 6). Ecosystem health of Jusucheon Stream (fish assessement index) evaluated using fish was assessed as very good (5 stations) and good (1 station). The main inhabit of L. costata was the middle-lower stream of aquatic plants habitats with mud bottoms, very slow water velocity and water depth 40~80 cm. The age groups for L. costata (female) estimated by the frequency distribution of total length in the spawning season (May) indicated that the 24~37 mm is 1-year old, the 38~51 mm group is 2-year old, the 52~63 mm is 3-year old, 64~77 mm is 4-year old and the 80~91 mm is more than 5-year old. Finally, characteristics of fish communities, habitat characteristics and the age of Lefua costata in the Jusucheon was discussed.

Feeding Habits of the Largehead Hairtail, Trichiurus japonicus in the Yellow Sea of Korea (우리나라 서해에서 출현하는 갈치(Trichiurus japonicus)의 식성)

  • Seong, Gi Chang;Kim, Do-Gyun;Kang, Da Yeon;Jin, Suyeon;Kim, Hoseung;Soh, Ho Young;Baeck, Gun Wook
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.179-185
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    • 2022
  • The feeding habits of Largehead hairtail Trichiurus japonicus was studied using 377 specimens collected in the Yellow Sea of Korea. The specimens ranged from 4.5 to 33.7 cm in Anal length (AL). T. japonicus consumed mainly a piscivore, such as Engraulis japonicus [percent index of relative importance (%IRI) =74.1%]. We calculated the trophic level as 3.84 for T. japonicus. Fishes were the main prey items for all seasons. The main fish prey during autumn and winter was E. japonicus, whereas those during spring and summer was Larimichthys polyactis. Fishes were the main prey items for all size groups (<15 cm, 15~20 cm, 20~25 cm, ≥25 cm). T. japonicus also showed size-related dietary shift from Spratelloides gracilis and E. japonicus to L. polyactis and T. japonicus. As the anal length of T. japonicus increased, the mean number of preys per stomach (mN/ST) and the mean weight of preys per stomach (mW/ST) tended to increased (One-way ANOVA, P<0.05). Seasonal and size-related shifts in dietary composition were investigated by PERMANOVA analysis, which showed significant variations among size classes and seasons.

Statistical Analysis of Amylose and Protein Content in Breeding Line Rice Germplasm Collected from East Asian Countries Based on Near-infrared reflectance spectroscopy (근적외선분광분석에 의한 육성계통 벼 유전자원의 아밀로스 및 단백질 성분함량에 관한 통계분석)

  • Oh, Sejong;Choi, Yu Mi;Yoon, Hyemyeong;Lee, Sukyeung;Lee, Myung Chul;Shin, Myoung-Jae;Yoo, Eunae;Hyun, Do Yoon;Chae, Byungsoo
    • Korean Journal of Breeding Science
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    • v.51 no.4
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    • pp.298-317
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    • 2019
  • A statistical analysis of 9,771 non-glutinous rice in breeding line germplasm collected from Korea (2,836), China (2,136), Japan (1,219), and the Philippines (1,213) was conducted using normal distribution, variability index value (VIV), analysis of variation (ANOVA) and Ducan's multiple range test (DMRT) based on the data obtained from NIRS analysis. According to the normal distribution, the average protein content was 7.9%, and non-glutinous rice ranging over 10% amylose had 23.6% average content. Most resources were between 5.3 and 10.5% in protein content, and 15.7 and 31.5% in amylose content. The VIV was 0.54 for protein, and 0.83 for amylose. The average amylose content was 25.18%, 24.54%, 22.08%, and 21.47% in Filipino, Chinese, Korean, and Japanese resources, respectively, wheereas the average protein content was found to be 8.19%, 7.79%, 7.58%, and 7.42% in Filipino, Chinese, Korean, and Japanese resources, respectively. The ANOVA of amylose and protein content showed significant differences at the level of 0.01. The F-test value was 412.2 for amylose content, and 108.4 for protein when compared with the critical value of 3.78. The DMRT of amylose and protein content showed significant differences (p<0.01) among resources from different countries. The Filipino resources had the highest level of amylose and protein content, whereas; the lowest level of amylose and protein content were found in Japanese when compared with resources of other origins. These results are recommended as helpful materials in the field of breeding.

The Study on the Factors of film's Processing Fluency Inducing Film's Preference Fluency (영화의 선호도 유창성에 영향을 미치는 영화의 처리 유창성 요인에 관한 연구)

  • Choi, Nak Hwan;Lim, Ahyoung
    • Asia Marketing Journal
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    • v.13 no.4
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    • pp.29-54
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    • 2012
  • Recently, as film has been admitted as the artistic merit and higher value-added business, the past studies on film have been lively carried on various fields. Especially, the film business is thought to be value-added business and has explored the causes of influencing spectators. However, there are no researches enough to explain what induces the choice and diffusion of the film. The film is not only hedonic products but also typical example of experiential products. So how to process film formation plays important roles in explaining the procedures of forming preference on the film and the movies spread more widely. But the great part of study of films has been concentrated on exploring hedonic factors of influencing spectator's choice. Until now there is not enough study for the relationship between experiential information processing and film preference. To explain film's preference, our study focuses on preference fluency and processing fluency that can provide an insight for our question about the relationship. In this article, to explain the procedures of processing experiential information and forming preference on the film, our study focuses on finding the relationship between film's processing fluency and film's preference fluency and explores the factors that affect film's processing fluency. To achieve the goal of this study, we distinguish factors of film's conceptual fluency from factors of film's perceptual fluency and explore the paths from the factors to film's preference fluency. The factors which have effects on perceptual fluency are hypothesized to be distinction of image expression, distinction of sound expression, correspondence between actors' image and their role. The factors which have effect on conceptual fluency are supposed to be well-organized story, suitability of lines expression. The experiments in which students were sampled at 'C' university were conducted in 2010 (december). Data collection was proceeded through questionnaires. We test the hypothesized model by using structural equation modeling(Amos 17.0). The fit indices for the model are as follows : x2=416.266(df=213, p=0.00), GFI=0.855, AGFI=0.812, RMSEA =0.069, IFI=O.925, CFI=0.920, TLI=0.905. According to the guidelines, there is evidence that our measurement model fits data. The results of empirical study are as follows. The path from film's perceptual fluency to film's preference fluency is supported(estimate: 0.223, C.R: 2.641). The path from film's conceptual fluency to film's preference fluency is supported(estimate: 0.397, C.R: 4.863). The path from distinction of image expression to film's perceptual fluency is not supported(estimate: 0.113, C.R: 1.665). The path from distinction of sound expression to film's perceptual fluency is supported (estimate: 0.190, C.R: 2.042). The path from correspondence between actors' image and their role to film's perceptual fluency is supported(estimate: 0.686, C.R: 5.566). The path from well-organized story to film's conceptual fluency is supported(estimate: 0.396, C.R: 4.023). The path from suitability of lines expression to film's conceptual fluency is supported(estimate: 0.536, C.R: 5.441). Concludingly, our study explored the influencing factors of film's processing fluency inducing film's Preference fluency. First, film's perceptual fluency and film's conceptual fluency have positive effects on film's preference fluency. Second, distinction of image expression is not significant on film's perceptual fluency, but distinction of sound expression and image's correspondence of actors' image and their role have positive effects on film's perceptual fluency. Lastly, well-organized story and suitability of lines expression have positive effects on film's conceptual fluency.

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Cultural Characteristics and Artificial Cultivation of Edible Mushroom, Clitocybe maxima (흰깔대기버섯의 배양적 특성 및 인공재배에 관한 연구)

  • Kim, M.K.;Kim, D.U.;Kang, H.W.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.1
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    • pp.35-47
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    • 2018
  • A edible mushroom, Clitocybe maxima (Lentinus giganteusis) commercially cultivated in China and Taiwan. However, the researches of cultivation and cultural characteristics were not reported in Korea. In this study, we conducted on cultural characteristics and artificial cultivation of C. maxima. Six isolates were collected from China(3 isolates, commercial strain), Taiwan(1 isolate, commercial strain) and Korea(2 isolates, wild type). C. maxima and L. giganteus collected in China and Taiwan, respectively, are the same in China and are estimated to be of the same species as cultured characteristics. The mycelial growth of the collected strains was not significantly different in agar medium but it showed the best growth in YPMG in liquid culture. Optimum temperature for mycelial growth and induction of fruit body were 25℃ and 30℃, respectively. In order to artificial cultivation of C. maxima, cultural characteristics and artificial cultivation were carried out using agricultural by-products and forestry by-products materials. Mycelial growth was suitable in rice straw, cottonwood sawdust, corncob and rice seed medium, and it was selected as a cultivation medium. The suitable medium for artificial cultivation of C. maxima was selected to mixed medium 2(compounding ratio(v/v): 55% of hardwood sawdust, 5% of cottonseed pellets, 10% of cottonseed, 15% of beet pulp, 15% of swollen rice husks). It took about 30 days to be able to harvest, it was faster than oyster mushrooms. The cultivation period was about 30days. A isolate, CMA-002 was not initiation to fruit body primordiuma on the used cultivation substrate. Other 5 isolates were initiate and development to fruit body on the substrate used in this study. The strain CMA-003 was initiated to be fruiting body by 8~10 days after induction of fruiting body in all of the substrates. Isolate CMA-003 was generate to a bundle fruit body. Other isolates, however, were form fruit body individually. The CMA-003 strain was likely highly recommendable strains for farming. The optimum conditions for the induction and growth of C. maxima fruit body were 25~30℃, 8 hr illumination per day with white fluorescent lamp, 90~95% relative humidity, and 1,500 ppm of CO2 concentration in a cultivation room.

Antioxidant and Anti-obesity Effects of Mulberry (Morus alba L) Fermented withLactobacillus plantarum JCM 1149 or Pichia kudriavzevii Atz-EN-01 (Lactobacillus plantarum JCM 1149와 Pichia kudriavzevii Atz-EN-01를 이용한 오디 발효액의 항산화 및 항비만 효과)

  • Ji-Young Lee;Su-Bin Oh;So-Yoon Joo;Sang-Kyu Noh;Dae-Ook Kang
    • Journal of Life Science
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    • v.33 no.10
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    • pp.797-807
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    • 2023
  • To improve the functionality of mulberry, samples were fermented with Lactobacillus plantarum JCM 1149 (LP) or Pichia kudriavzevii Atz-EN-01 (PK), and their antioxidant and anti-obesity activities were compared to those of unfermented mulberry. After fermenting for 60 hr, the total polyphenol and flavonoid content of the PK-fermented mulberry (PKFM) and LP-fermented mulberry (LPFM) was 1.5-fold and 2-fold higher, respectively, while the total anthocyanin content was 1.3-fold and 1.5-fold higher in the PKFM and LPFM, respectively. DPPH radical scavenging activity was found to be 16.3% higher (86% vs. 100%) after PK fermentation and 8.1% higher (86% vs. 93%) after LP fermentation. The lipase inhibitory activity of the LPFM and PKFM was 62.9% and 52.5%, respectively. 3T3-L1 preadipocytes were treated with unfermented mulberry, LPFM, or PKFM at 200, 400, or 800 ㎍/ml and stained with oil-red-O. A slight difference in the staining was observed in samples treated with 400 ㎍/ml. However, treatment with 800 ㎍/ml significantly reduced staining compared to the control, and the LPFM exhibited relatively higher adipogenesis inhibitory activity than the PKFM. Blood triglyceride content increased by 9.5% in the high-fat diet group, but decreased by 17.1% in the control group, 37.1% in the LPFM group, and 41.6% in the PKFM group. The blood triglyceride content of the LPFM group decreased by 43.1% and 21.4% compared to the high-fat diet group and the control group, respectively, and that of the PKFM group decreased by 48.6% and 28.9% compared to the same groups. In conclusion, the results indicate that fermented mulberry has increased antioxidant activity, lipase inhibitory activity, and adipogenesis inhibition activity, and decreased blood triglyceride content compared to unfermented mulberry.

Study on the Suitable Fermentation Period of Berry Sawdust for the Development of Protaetia brevitarsis Larva (흰점박이꽃무지 유충의 발육에 적합한 베리류톱밥 발효기간에 관한 연구)

  • Ju-Rak Lim;Sang-sik Lee;Eun-Jin Lee;Woong Kim;Chang-hak Choi
    • Korean journal of applied entomology
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    • v.62 no.3
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    • pp.183-191
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    • 2023
  • After examining the temperature change in berry sawdust during the fermentation period and the developmental characteristics of Protaetia brevitarsis larvae according to the fermentation period, it was found that the fermentation speed was faster than with oak sawdust and a fermentation period of 50-70 days was the most suitable for larval growth. During the fermentation period, the temperature change in sawdust was quickly fermented as berry sawdust passed a high temperature of more than 60℃ in the early stages of fermentation compared to fermentation speed of oak sawdust, and stabilized within 60 days. In individual breeding, the total growth period of P. brevitarsis larvae by fermentation period was the longest at 104.8 days after fermentation for 30 days, which was a significant difference. There was no difference in the growth period between 50 and 90 days of fermentation. Oak sawdust tended to be the same as berry sawdust, but the larval development period was approximately 30 days. The developmental period by larval stage differed greatly between berries and oak sawdust. The weight of larvae according to the fermentation period of sawdust was the lowest after 90 days of fermentation, with a long fermentation period for both berry and oak sawdust, and there was no difference in the 30-70 days fermentation periods. The survival rate was more than 95% in individual breeding, and there was no difference among the fermentation periods. However, in group breeding, the survival rate was lower than that of individual breeding and was lowest at 90 days of fermentation. In group breeding, the ratio of larvae weighing ≥ 2.5 g, which is the standard for shipment, was 85% after 30-70 days of fermentation of berry sawdust, but was lower at 70% after 90 days of fermentation, which was a significant difference. The survival rate with oak sawdust showed the same trend, which was approximately 10% lower than that with sawdust. In addition, the larvae from the berry sawdust weighed > 2.5 g in 40-60 days, while it took 75-105 days with oak-fermented sawdust.

Growth of Intestinal Bacteria and Intestinal Inflammation of Sprout Extract from Common Buckwheat and Tartary Buckwheat (일반메밀과 쓴메밀의 새싹 추출물의 장내 유익균 증식 및 염증조절 효능 평가)

  • Su Jeong Kim;Hwang Bae Sohn;Jong Won Kim;Sanghyun Lim;Jong Nam Lee;Su Hyoung Park;Jung Hwan Nam;Do Yeon Kim;Ye Jin Lee;Dong Chil Chang;Yul Ho Kim
    • Korean Journal of Plant Resources
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    • v.36 no.5
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    • pp.455-468
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    • 2023
  • We aimed to assess the potential growth-promoting effects of buckwheat sprout on intestinal bacteria and their anti-inflammation effects in a cellular model of intestinal inflammation. The growth of Bifidobacterium longum ssp. infantis BT1 was enhanced with the addition of the sprout extract of tartary buckwheat. Further, in the inflammatory model cells cultured with Raw 264.7 cells were treated with buckwheat sprout including each 10 probiotics before the addition of lipopolysaccharide (LPS) to induce inflammation in Raw 264.7 cells. Buckwheat sprout in both Bifidobacterium longum ssp. infantis BT1 and Lacticaseibacillus paracasei LPC5 significantly reduced the production of NO and PGE2. The above results indicate that buckwheat sprout extract which contains with various physiologically active substances such as rutin, quercetin, and choline is effective in suppressing NO and PGE2 production, which are inflammation-related indicators. The present study suggests that buckwheat sprout could induce positive effects on the intestinal beneficial bacteria and in anti-inflammation.

Comparison of the Growth Performance of 12 Crossbred Korean Native Chickens and Commercial Layer from Hatch to 16 Weeks (12개의 토종닭 교배조합과 실용 산란계의 육성기 성장능력 비교)

  • Eunsoo Seo;Myunghwan Yu;Elijah Ogola Oketch;Shan Randima Nawarathne;Nuwan Chamara Chathuranga;Bernadette Gerpacio Sta. Cruz;Venuste Maniraguha;Jun Seon Hong;Doo Ho Lee;Minjun Kim;Jung Min Heo
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.303-310
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    • 2023
  • The current study was conducted to compare the effect of crossbred on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 873 one-day-old chicks were divided into twelve crossbreds (i.e., CFCK, CFYC, CFYD, CKCF, CKYC, CKYD, YCYD, YCCF, YCCK, YDCF, YDCK, and YDYC) and commercial layer (Hy-Line Brown) were obtained as a counterpart in the study. All the birds are raised in battery cages (76 × 61 × 46 cm3) and then raised until 14 weeks and subsequently moved to layer battery cages (60 × 25 × 45 cm3) and raised until 16 weeks. The body weight and viability were measured biweekly from hatching to week 16. The week of 16, body weight range was about 1,010.24 to 1,411.77 g. The body weight of specific crossbreeds (i.e., CKCF, YCYD, and YDYC) was found to be comparable to that of Hy-Line Brown). The viability hatch to week 14 range was about 55 to 100% and however week 14 to 16 range was 80 to 100%. The crossbred (i.e., CKCF) recorded superior (P<0.05) viability throughout the whole experiment period, even compared with Hy-Line Brown (100% vs 96%). Our results are indicating that crossbreds Korean native chicken including CKCF, and YDYC has the potential to enhance key features of laying hens during the growing phase like optimal body weight and higher viability.