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Development of Physiological and Biochemical Bioindicators of Barnacle, Megabalanus rosa for Marine Pollution Assessment (해양오염 평가를 위한 빨강따개비(Megabalanus rosa)의 생리생화학적 오염지표의 개발)

  • LEE Jang-Won;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.276-282
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    • 2003
  • Using Megabalanus rosa a sessile filterfeeder, its scope for growth (SFG) by analysis of energy budget were examined and free amino acids composition of whole body tissue were analyzed under the exposure to different concentrations of Hg Cu and Cd. The $96\;hr-LC_{50}$ of the barnacle after 96 hr exposure to Hg, Cu, and Cd were 0.220, 0.269 and $1.380\;mgL^{-1}$, respectively. Hg and Cu showed stronger toxicity than Cd, while Hg and Cu had similar influence on the survival of the barnacle. SFG of the barnacles exposed to sublethal concentrations of mercury was 18.936 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of mercury concentration the SFG remarkably reduced to 0.041 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration of Hg. In the case of Cu, the SFG was 29.841 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of concentration, the SFG remarkably reduced to -8.304 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration. In Cd the SFG was 15.852 $Jgdrywt.^{-1}hr^{-1}$ in control group, and as increasing concentration, the SFG remarkably reduced to -19.490 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}.$ Content of free amino acid (FAA) of whole body tissue of the barnacle was 45084 $mgkg^{-1}$ in control group, but it was reduced remarkably to 28,130, 37,500 and 37,106 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ concentration of Hg and Cu, and 0.4 $mgL^{-1}$of Cd, respectively. Sum of threonine + serine was 1,334 $mgkg^{-1}$ if control but reduced remarkably to 1,223, 849 and 888 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ of Hg and Cu, and 0.4 $mgL^{-1}$ of Cd, respectively.

Effects of Plant Growth Regulators and Anti-oxidants on Rapid Multiplication of Cymbidium kanran (한란의 급속증식을 위한 생장조절물질과 항산화제 처리효과)

  • 소인섭;최지용;고태신;이종석
    • Journal of Life Science
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    • v.8 no.5
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    • pp.520-525
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    • 1998
  • Effects of plant growth regulators and anti-oxidants for rapid multiplication of Cymbidium kanran were investigated. The best gelling agent was 2.5 g/1 gelrite which needed less quantity (about 28%) and half price than 9 g/1 chemi-cal agar. Undefined edible agar was only a little bit worse than chemical agar in growth, but the price was half as much as the latter. The higher concentration of BA and NAA, the deeper browning of medium that prevented from performing its functions of plant growth regulators. Polyvinylpyrrolidone (M.W. 40,000) was the most effective anti-oxidant other than ascorbic acid, aspartic acid, and rutin in protecting the browning of medium, enhancing the effect of plant growth regulators, and thus prolonging the subculture cycle. Vigorous seedlings were obtained by 0.1∼1.0 mg/1 BA,0.1 mg/1 NAA and 1 g/1 polyvinylpyrrolidone treatments. Therefore, the best result for growth and econo-mic aspects in rhizome culture of Cymbidium kanran were obtained by using MS basal medium with 2.5 g/1 gelrite, 1 g/1 polyvinylpyrrolidone, 0.1∼1.0 mg/1 BA and 0.1 mg/1 NAA.

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Studies on the Durable Softners (I) -Synthesis of Alkyl Imidazoline Derivatives- (내구성유연제에 관한 연구 (I) -알킬이미다졸린 유도체의 합성-)

  • Park, Hong-Soo;Kim, Young-Geun;Pyoun, Moo-Sil
    • Applied Chemistry for Engineering
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    • v.1 no.2
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    • pp.197-206
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    • 1990
  • 1, 2-Disubstituted imidazolines, such as 1-behenoyl-aminoethyl-2-heneicosylimidazoline(BHI), 1-behenoylbis(aminoethyl)-2-heneicosylimidazoline(BBI), and 1-behenoyltris(aminoethyl)-2-henoicosylimidazoline(BTI) were synthesized by reacting 3 kinds of polyalkylene polyamines with behenic acid to provide the softness to imidazoline ring.1, 2-Disubstituted imidazolinium chlorides, such as 1-behenoyl-aminoethyl-1-glycidyl-2-heneicosylimidazolinium chloride(BHIC), 1-behenoylbis(aminoethyl)-1-glycidyl-2-henoicosylimidazolinium chloride(BBIC), and 1-behenoyltris(aminoethyl)-1-glycidyl-2-henoicosylimidazolinium chloride(BTIC) were prepared by quaternizing 1, 2-disubstituted imidazoline compounds. The optimum condition for the preparation of BHI, BBI, and BTI was 8 hrs at $210^{\circ}C$. In the synthesis of BHIC, the completely quaternized product was obtained by reacting BHI-epichlorohydrin with 1:1.3 mole ratio, and mole ratios higher than 1:1.5 were required in the preparation of BBIC and BTIC, BHICS, BBICS, and BTICS softners form BHIC, BBIC, and BTIC was treated to acrylic fiber. It was found that were good softners as the softening property was measured.

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Volatile Compounds Formed by Thermal Degradation of Ascidian Tunic Carotenoids (우렁쉥이 껍질 카로테노이드의 가열분해로 생성되는 휘발성 화합물의 특성)

  • Ho, Chi-Tang;Choi, Byeong-Dae
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.519-524
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    • 1997
  • As an investigation for utilization of ascidian tunic carotenoids as a food color additives, we attempted to collect the volatile thermal degradation compounds from ascidian tunic carotenoids. Oxygenate volatile compounds were extracted by simultaneous distillation and extraction/concentration apparatus and analyzed by gas chromatography and mass spectrometery. Total 63 compounds were identified and some of them were caused by thermal degradation. They included 1,3,5-trimethylbenzene, 3,5,5-trimethyl-3-cyclohexen-1-ol, 3,5,5-trimethyl-3-cyclohexen-1-one, 1,1,2,3-tetramethyl-2-cyclohexen-5-ol, 1,1,2,3-tetramethyl-2-cyclohexen-5-one, 2,3,4,4-tetramethyl-6-hydroxy-2-cyclohexene-1-one, 1,2,3,8-tetrahydro-3,3,6-trimethyl-1-naphtol, dihydroacetinidolide, ${\beta}-ionone$, 2-(1,1,5-trimethyl-3-hydroxy-5-cyclohexen-6-yl)-1-tolylethene, 2,6-dimethyl-8-(1,1,5-trimethyl-3-hydroxy-5-cyclohexen-6-yl)-1,3,5-octatriene-7-yne. Proposed mechanism of formation of some compounds as thermal degradation products of ascidian tunic carotenoids are provided.

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Studies on Synthesis of 1,2- and 1,3-Diamine Derivatives: Synthesis and Molecular Structure of 3-(N-Benzylamino)-4,5-dihydro-1,2,5-thiadiazole 1,1-dioxides (1,2- 그리고 1,3-디아민 유도체의 합성에 관한 연구(Ⅰ): 3-(N-벤질아미노)-4,4-디히드로-1,2,5-티아디아졸 1,1-디옥시드의 합성과 분자구조)

  • Lee, Chai-Ho;Kim, Eui-Sung;Chung, Bong-Young;Lee, Yeong-Haeng
    • Journal of the Korean Chemical Society
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    • v.40 no.7
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    • pp.526-530
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    • 1996
  • 3-(N-Alkylamino)-4,5-dihydro-1,2,5-thiadiazole 1,1-dioxides have been prepared by the treatment of 3-imino-1,2,5-thiadiazolidine 1,1-dioxides with alkylamines. The selected spectral properties of these heterocycles are detailed, as well as an X-ray crystallographic structure of a representative member of these compounds. 3-(N-Benzylamino)-4,5-dihydro-4-(4'-methoxyphenyl)-1,2,5-thiadiazole 1,1-dioxide crystallyzes in space group $P2_1a$, monoclinic, with $a=22.512(3)(1)\AA$, $b=9.831(2)\AA$, $c=7.194(4)\AA$, $\beta=96.65(2)$, and Z=4.

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Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression

  • Kumar, Shiv;Umair, Zobia;Kumar, Vijay;Lee, Unjoo;Choi, Sun-Cheol;Kim, Jaebong
    • BMB Reports
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    • v.52 no.6
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    • pp.403-408
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    • 2019
  • Dorsoventral patterning of body axis in vertebrate embryo is tightly controlled by a complex regulatory network of transcription factors. Ventx1.1 is known as a transcriptional repressor to inhibit dorsal mesoderm formation and neural differentiation in Xenopus. In an attempt to identify, using chromatin immunoprecipitation (ChIP)-Seq, genome-wide binding pattern of Ventx1.1 in Xenopus gastrulae, we observed that Ventx1.1 associates with its own 5'-flanking sequence. In this study, we present evidence that Ventx1.1 binds a cis-acting Ventx1.1 response element (VRE) in its own promoter, leading to repression of its own transcription. Site-directed mutagenesis of the VRE in the Ventx1.1 promoter significantly abrogated this inhibitory autoregulation of Ventx1.1 transcription. Notably, Ventx1.1 and Xcad2, an activator of Ventx1.1 transcription, competitively co-occupied the VRE in the Ventx1.1 promoter. In support of this, mutation of the VRE down-regulated basal and Xcad2-induced levels of Ventx1.1 promoter activity. In addition, overexpression of Ventx1.1 prevented Xcad2 from binding to the Ventx1.1 promoter, and vice versa. Taken together, these results suggest that Ventx1.1 negatively regulates its own transcription in competition with Xcad2, thereby fine-tuning its own expression levels during dorsoventral patterning of Xenopus early embryo.

The Study on the Seasonal Variation of Microbial Community in Kyeonggi Bay, Korea II. Nano-and Microzooplankton (경기만 수역에서 미세생물 군집의 계절적 변동 연구 II. 미소형 및 소형 동물플랑크톤)

  • 양은진;최중기
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.78-93
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    • 2003
  • To investigate seasonal variation and structure of the microbial community in Kyeonggi Bay, abundance and carbon biomass of nano-and micrzooplankton were evaluated in relation to size fractionated chlorophyll-a concentration, through the monthly interval sampling from December 1997 to November 1998. Communities of nano-and microzooplankton were classified into 4 groups such as heterotrophic nanoflagellate(HNF), ciliates, heterotrophic dinoflagellates(HDF) and zooplankton nauplii. Abundance and carbon biomass of HNF ranged from 380 to 4,370 cells ml-1(average 1,340$\pm$130 cells ml-1) and from 0.63 to 12.4 $\mu\textrm{g}$C 1-1(average 4.35$\pm$0.58 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of ciliates ranged from 331 to 44,571 cells ml-1(average 3,526$\pm$544 cells ml-1) and from 1.3 to 119.7 $\mu\textrm{g}$C 1-1(average 13.7$\pm$3.0 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of HDF ranged from 88 to 48,461 cells 1-1(average 9,034$\pm$2,347 cells 1-1) and from 0.05 to 54.05 $\mu\textrm{g}$C 1-1(average 6.9$\pm$2.4 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of zooplankton nauplii ranged from 5 to 546 indiv. 1-1(average 83$\pm$15 indiv. 1-1) and from 0.17 to 43.2 $\mu\textrm{g}$C 1-1(average 6.3$\pm$1.2 $\mu\textrm{g}$C 1-1), respectively. Eash component of microbial biomass was not different from tidal cycle except tintinnids group. Depth integrated nano-and microzooplankton biomass ranged from 124 to 1,635 mgC m-2(average 585$\pm$110 mgC m-2) and was highest in March and May. The relative contribution of each component to the nano-and microzooplankton showed difference according to seasons. Community structure of nano-and microzooplankton was dominated by planktonic ciliate group. During the study period, carbon biomass of nano-and microzooplankton was strongly positively correlated with size fractionated chlorophylla-a. It implied that prey-predator relationship between microzooplankton and phytoplankton was important in the pelagic ecosystem of Kyeonggi Bay.

Synthesis and Antifungal Activities of 1-[(2-Phenyl-1,3-benzodioxol-2-yl)methyl]-1H-imidazole Derivatives (1-[(2-Phenyl-1,3-benzodioxol-2-yl)methyl]-1H-imidazole 유도체의 합성 및 항진균작용)

  • 서정진;김재규;강희일
    • YAKHAK HOEJI
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    • v.35 no.4
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    • pp.308-313
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    • 1991
  • The synthesis of 1-[(2-phenyl-1, 3-benzodioxol-2-yl)methyl]-1H-imidazole derivatives is described starting with 2-bromoacetophenones and catechols. 1-[(2-Phenyl-1, 3-benzodioxol-2-yl)methyl]-1H-imidazole (9-$_{12}$) showed potent broad-spectrum activity, not only against Candida species but also Cr. neofortnans, S. cerevisiae and T. mentagrophytes.

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2009 Pandemic Influenza A(H1N1) Infections in the Pediatric Cancer Patients and Comparative Analysis with Seasonal Influenza (소아암 환자에서 2009 대유행 인플루엔자 A(H1N1) 감염의 임상적 고찰 및 계절 인플루엔자와의 비교 분석)

  • Choi, Soo Han;Yoo, Keon Hee;Ahn, Kangmo;Sung, Ki Woong;Koo, Hong Hoe;Kim, Yae Jean
    • Pediatric Infection and Vaccine
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    • v.19 no.2
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    • pp.61-70
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    • 2012
  • Purpose: This study was performed to compare the clinical characteristics of 2009 pandemic influenza A(H1N1) [A(H1N1) pdm09] and seasonal influenza A infection in the pediatric cancer patients. Methods: A retrospective review was performed in the pediatric cancer patients who had confirmed A(H1N1)pdm09 infection at Samsung Medical Center from August 2009 to February 2010. For the comparison, the medical records of pediatric cancer patients with seasonal influenza A from January 2000 to May 2009 were reviewed retrospectively. Results: Eighty-two A(H1N1)pdm09 infections were confirmed in the pediatric cancer patients. Ten patients (12.2%) developed complicated clinical course by lower respiratory infections or extrapulmonary infections; 4 pneumonia, 1 bronchitis, 1 pericarditis with pneumonia, 1 encephalitis with pneumonia, 2 meningitis and 1 pericarditis. Three patients received mechanical ventilator and ICU care. Three pediatric cancer patients (3.7%) died. The risk factors related to complicated A(H1N1)pdm09 infections were date of infection (44-45th week 2009) and nosocomial infection. When comparing with previous seasonal influenza A infections, more prompt and aggressive antiviral therapy was given in A(H1N1)pdm09 infections. Conclusion: The A(H1N1)pdm09 infections caused a various clinical manifestations including fatal cases in pediatric cancer patient during pandemic season. There was no significant difference in clinical course between influenza A(H1N1)pdm09 and seasonal influenza A infections except the antiviral treatment strategy.

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Influence of Irrigation Times, Soil Treatment and Drainage in Indoor on the Growth Response of Cyrtomium falcatum Ferns Korea Native (실내에서 관수주기, 토양처리, 배수층이 자생 도깨비고비의 생육에 미치는 영향)

  • Ju, Jin Hee;Bang, Kwang Ja
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.8 no.1
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    • pp.73-78
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    • 2005
  • It was aimed to promote Cyrtomium falcatum as a material for interior landscape by validating it indoor adaptability in the indoor environment, especially irrigation times, soil treatment and drainage level. Irrigation times were 2 times per week and 7 times per week. Soil treatment and drainage level were carried out drainage-peatmoss : vermiculite : perlite=1 : 1 : 1(D-PVP), peatmoss : vermiculite : perlite=1 : 1 : 1(PVP), drainage-saprolite : leaf mold=1 : 1(D-SL) and saprolite : leaf mold=1 : 1(SL). 1. Top of growth was better with irrigation 7 times per week than irrigation 2 times per week but indoor adaptability was decreased and shown yellowish green. 2. In case of soil treatment, growth was better with saprolite : leaf mold=1 : 1 but indoor growth adaptability was decreased than peatmoss : vermiculite : perlite=1 : 1 : 1. 3. Plant height and blade length were increased under non-drainage treatment but indoor adaptability, number of new fronds and number of sporophyll were decreased under drainage treatment, regardless of irrigation times and soil treatment. 4. Photosynthetic rate(Pn) was the highest in the drainage-peatmoss : vermiculite : perlite=1 : 1 : 1 treatment of irrigation 2 times per week and was the lowest in the saprolite : leaf mold=1 : 1 treatment of irrigation 7 times per week.