• Molecules and Cells
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• 제43권12호
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• pp.975-988
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• 2020

Hypoxia plays important roles in cancer progression by inducing angiogenesis, metastasis, and drug resistance. However, the effects of hypoxia on long noncoding RNA (lncRNA) expression have not been clarified. Herein, we evaluated alterations in lncRNA expression in lung cancer cells under hypoxic conditions using lncRNA microarray analyses. Among 40,173 lncRNAs, 211 and 113 lncRNAs were up- and downregulated, respectively, in both A549 and NCI-H460 cells. Uroplakin 1A (UPK1A) and UPK1A-antisense RNA 1 (AS1), which showed the highest upregulation under hypoxic conditions, were selected to investigate the effects of UPK1A-AS1 on the expression of UPK1A and the mechanisms of hypoxia-inducible expression. Following transfection of cells with small interfering RNA (siRNA) targeting hypoxia-inducible factor 1α (HIF-1α), the hypoxia-induced expression of UPK1A and UPK1A-AS1 was significantly reduced, indicating that HIF-1α played important roles in the hypoxia-induced expression of these targets. After transfection of cells with UPK1A siRNA, UPK1A and UPK1A-AS1 levels were reduced. Moreover, transfection of cells with UPK1A-AS1 siRNA downregulated both UPK1A-AS1 and UPK1A. RNase protection assays demonstrated that UPK1A and UPK1A-AS1 formed a duplex; thus, transfection with UPK1A-AS1 siRNA decreased the RNA stability of UPK1A. Overall, these results indicated that UPK1A and UPK1A-AS1 expression increased under hypoxic conditions in a HIF-1α-dependent manner and that formation of a UPK1A/UPK1A-AS1 duplex affected RNA stability, enabling each molecule to regulate the expression of the other.

• 한국동물학회지
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• 제7권1호
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• pp.6-8
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• 1964

1. A total of 260 individuals of carp was collected from Nakdong and Hyungsan Rivers. 2. The normal dental form of carp is 3, 1, 1-1, 1, 3, but the author observed abnormal dental forms (3, 2 or 1, 1, (+1)-1, 1 or 2, 3). 3. Variation ratios of dental forms are : A. Normal form; 3, 1, 1-1, 1, 3..........88.85% B. Abnormal forms; a. 3, 2, 1-1, 1 or 2, 3..........2.31% b. 3, 1, 1, 1-1, 1 or 2, 3..........5.00% c. 3, 2, 1, 1-1, 1 or 2, 3..........3.07% d. with 2 or 3 bone processes..........0.77% 4. The ratios among the length of first row (I), length of second tooth in first row ($I_{-2}$) and mid line(M) are as follows; 5. The growth of bone process beside second row tooth is not concerned with the body growth. 6. The number of the groove on second tooth in first row increases according to the growth of body.

• 약학회지
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• 제31권1호
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• pp.14-18
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• 1987

2-Substituted-3, 4-dihydro-2H-1, 2-benzothiazin-4-one 1, 1-dioxide 6 was reatced with magnesium methyl carbonate to form magnesium chelate 7, which could be hydrolized in cold hydrochloric acid solution to give 2-substituted-4-hydroxy-2H, 1, 2-benzothiazine-3-Carboxylic acid 1, 1-dioxide 2 in good yield.

• 한국콘텐츠학회논문지
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• 제17권7호
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• pp.648-663
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• 2017

아시아인종에서 GSTM1/GSTT1 유전자 다형성과 자가면역질환과 관련된 감수성을 검증하기 위해, 2015년 12월까지 EMBASE, Google, KISS, MEDLINE, PubMed에 발표된 18편의 논문들을 메타분석에 인용하였다. GSTM1/GSTT1 유전자의 null, present 유형을 개별적으로 분석하였다. 전체 인구에서 GST 다형성과 자가면역질환 사이에 연관성이 발견되었다(GSTM1, OR=1.334, 95% CI=1.137-1.567, p=0.000, GSTT1, OR=1.212, 95% CI=1.012-1.452, p=0.037). 아시아인종에서 자가면역질환, 특히 vitiligo와 아토피 피부염(p<0.05)에서 GSTM1 유전자와의 연관성이 있었고, RA와 SLE에서 GSTT1 유전자와의 연관성은 없었다(p>0.05). GSTM1 null 유형과 GSTT1 present 유형은 아시아인종에서 자가면역질환과 연관이 있었다(p<0.05). 자가면역질환과 GSTM1-GSTT1 다형성 조합 null 유형의 빈도는 대조군보다 높았다. 이와 같이, GSTM1-GSTT1 다형성 조합 null 유형이 아시아인종에서 자가면역질환의 위험 인자가 될 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1345-1349
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• 2014

Although many epidemiologic studies investigated the methylenetetrahydrofolate reductase (MTHFR) polymorphisms and their associations with esophageal cancer, definite conclusions could not be drawn. To clarify the effects of MTHFR polymorphisms on the risk of esophageal cancer, a meta-analysis was here performed in Chinese populations. A total of 16 studies including 3,040 cases and 4,127 controls were involved in this metaanalysis. Overall, significant associations were found between the MTHFR C677T polymorphism and esophageal cancer risk when all studies in Chinese populations were pooled into the meta-analysis (T vs. C, OR = 1.19, 95% CI = 1.06-1.34; TT vs. CC, OR = 1.35, 95% CI = 1.07-1.70; TT+ CT vs. CC, OR = 1.29, 95% CI = 1.08-1.54; TT vs. CC + CT, OR = 1.19, 95% CI = 1.03-1.37). In subgroup analyses stratified by ethnicity and source of controls, the same results were found in Kazakh (TT vs. CC, OR = 1.38, 95% CI = 1.02-1.87; TT + CT vs. CC, OR = 1.50, 95% CI = 1.03-2.18), in not stated populations (T vs. C, OR = 1.24, 95% CI = 1.08-1.42; TT vs. CC, OR = 1.47, 95% CI = 1.10-1.96; TT + CT vs. CC, OR = 1.30, 95% CI = 1.05-1.60; TT vs. CC + CT, OR = 1.32, 95% CI = 1.12-1.56), and in hospital-based studies (T vs. C, OR = 1.34, 95% CI = 1.19-1.51; TT vs. CC, OR = 1.81, 95% CI = 1.37-2.39; TT + CT vs. CC, OR = 1.51, 95% CI = 1.26-1.83; and TT vs. CC + CT, OR = 1.39, 95% CI = 1.13-1.70). In conclusion, this meta-analysis provides evidence that the MTHFR C677T polymorphism contributes to esophageal cancer development in Chinese populations.

• Archives of Pharmacal Research
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• 제29권7호
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• pp.535-540
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• 2006

Several novel 1-[2-(1H-tetrazol-5-yl) ethyl]-1H-benzo[d][1,2,3]triazoles (3a-h) have been synthesized by the condensation of 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) and appropriate acid chlorides. 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) was synthesized by reacting 3-(1H-benzo[d][1,2,3]triazol-1-yl)propanenitrile with sodium azide and ammonium chloride in the presence of dimethylformamide. The synthesized compounds were characterized by IR and PMR analysis. The titled compounds were evaluated for their in vitro antibacterial and antifungal activity by the cup plate method and anticonvulsant activity evaluated by the maximal electroshock induced convulsion method in mice. All synthesized compounds exhibited moderate antibacterial activity against Bacillus subtilis and moderate antifungal activity against Candida albicans. Compounds 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(4-aminophenyl)methanone 3d and 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(2-aminophenyl)methanone 3e elicited excellent anticonvulsant activity.

• 한국응용과학기술학회지
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• 제34권4호
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• pp.839-850
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• 2017

본 연구는 차세대 랫드에서 오메가 6(n-6)와 오메가 3(n-3) 지방산 비율이 서로 다른 식이(0, 1.2:1, 8:1, 19:1)의 섭취가 간에서 새롭게 합성 분비되는 glycerolipid의 대사적 분할에 관한 메카니즘을 구명하였다. 그 방법으로써 경정맥 캐뉼라를 부착한 랫드에서 생체모니터링 기법을 이용하였다. glycerolipid 대사에 의한 산화로써 나타난 $^{14}CO_2$ 배출율 범위는 8:1 이하 그룹에서 7.05-20.17%로 나타났으며 대조구에서 가장 낮았다(p<0.05). 총 glycerolipid는 대조구가 가장 높았으며 19:1, 8;1, 1.2:1 그룹의 순서로 높았다(p<0.05). 중성지방 분비량은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 36.35, 20.93, 13.72% 감소하였다(p<0.05). 총 glycerolipid에 대한 인지질의 분할 비율은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 1.38, 1.29, 1.17배 높았다(p<0.05). 총 glycerolipid에 대한 $^{14}CO_2$ 생성비율은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 1.61, 1.52, 1.29배 높았다(p<0.05).

• 공업화학
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• 제33권2호
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• pp.179-187
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• 2022

본 연구에서는 1,2-알칸디올계 화합물의 보존력과 친수성을 향상할 목적으로 12개의 탄소 사슬 길이와 아민기를 갖는 1,2-도데실아미노프로판디올(1,2-dodecylaminopropanediol, 1,2-DDAP)의 합성을 설계하였다. 1,2-DDAP는 40 ℃의 에탄올(ethanol) 용매에서 도데실아민(dodecyl amine, DDA)과 3-모노클로로-1,2-프로판디올(3-monochloro-1,2-propanediol, 3-MCPD)의 반응에 의해 제조하였으며, 그 수율과 순도는 1:0.8의 DDA:3-MCPD 몰 비와 2 h의 반응 조건에서 각각 약 56%와 98%였다. 1,2-DDAP의 항균 효과는 1,2-옥탄디올(1,2-octanediol, 1,2-ODIOL) 또는 1,2-데칸디올(1,2-decanediol, 1,2-DDIOL)과 비교하여 10~100배 낮은 농도에서 미생물에 대한 최소억제농도와 최소살균농도 값을 나타내었다. 1,2-DDAP의 친수성을 바탕으로 1,2-DDAP로 물에 난용성인 1,2-ODIOL 또는 1,2-DDIOL을 소량 첨가하여 혼합 보존제를 제조하였다. 항균력 시험에서 혼합 보존제는 1,2-DDAP 단독사용 대비 동등 이상의 미생물 억제효과를 나타내었다. 응용을 위해 로션(화장품 제형)에서 보존 효과를 확인한 결과, 1,2-DDAP는 1,2-ODIOL 또는 1,2-DDIOL과 비교하여 0.3~0.6배의 낮은 농도에서 유사한 항균력을 나타내었다. 따라서 1,2-DDAP의 단독 사용 및 1,2-ODIOL 또는 1,2-DDIOL을 소량 혼합하여 사용하는 것이 제품 내 보존제에 대한 좋은 대안으로 사용될 수 있을 것이다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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• pp.476-479
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• 2001

The microwave dielectric properties of $[(Pb_{1-x}Ba_{x})_{1/2}La_{1/2}](Mg_{1/2}Nb_{1/2})O_{3}$ ceramics were investigated. When $[(Pb_{0.9}Ba_{0.1})_{1/2}La_{1/2}](Mg_{1/2}Nb_{1/2})O_{3}$ ceramics were sintered at $1250^{\circ}C$ and $1350^{\circ}C$ for 2hr, the microwave dielectric properties were obtained $\varepsilon_{r}=64\sim80$, $Q{\times}f=11,800\sim18,000$. As a result, $[(Pb_{0.9}Ba_{0.1})_{1/2}La_{1/2}](Mg_{1/2}Nb_{1/2})O_{3}$ having $\varepsilon_{r}=80$, $Q{\times}f=11,800$ (at 4 GHz) was developed.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.476-479
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• 2001

The microwave dielectric properties of [(Pb$_{1-x}$ Ba$_{x}$)$_{1}$2/La$_{1}$2/](Mg$_{1}$2/Nb$_{1}$2/)O$_3$ ceramics were investigated. When [(Pb$_{0.9}$Ba$_{0.1}$)$_{1}$2/La$_{1}$2/](Mg$_{1}$2/Nb$_{1}$2/)O$_3$ ceramics were sintered at 125$0^{\circ}C$ and 135$0^{\circ}C$ for 2hr, the microwave dielectric properties were obtained $\varepsilon$$_{r}$=64~80, Qxf=11,800~18,000. As a result, [(Pb$_{0.9}$Ba$_{0.1}$)$_{1}$2/La$_{1}$2/](Mg$_{1}$2/Nb$_{1}$2/)O$_3$ having $\varepsilon$$_{r}$=80, Qxf=11,800 (at 4 GHz) was developed.ed.eveloped.ed.