• Journal of Microbiology
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• v.44 no.6
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• pp.641-648
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• 2006

We previously reported that Skp1, a component of the Skp1-Cullin-F-box protein (SCF) complex essential for the timely degradation of cell cycle proteins by ubiquitination, physically interacts with Bfa1, which is a key negative regulator of the mitotic exit network (MEN) in response to diverse checkpoint-activating stresses in budding yeast. In this study, we initially investigated whether the interaction of Skp1 and Bfa1 is involved in the regulation of the Bfa1 protein level during the cell cycle, especially by mediating its degradation. However, the profile of the Bfa1 protein did not change during the cell cycle in skp1-11, which is a SKP1 mutant allele in which the function of Skp1 as a part of SCF is completely impaired, thus indicating that Skp1 does not affect the degradation of Bfa1. On the other hand, we found that the skp1-12 mutant allele, previously reported to block G2-M transition, showed defects in mitotic exit and cytokinesis. The skp1-12 mutant allele also revealed a specific genetic interaction with ${\Delta}bfa1$. Bfa1 interacted with Skp1 via its 184 C-terminal residues (Bfa1-D8) that are responsible for its function in mitotic exit. In addition, the interaction between Bfa1 and the Skp1-12 mutant protein was stronger than that of Bfa1 and the wild type Skp1. We suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex. The interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.2
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• pp.579-583
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• 2012

Background: The SULT1A1 Arg213His polymorphism is reported to be associated with lung cancer risk. However, this relationship remains controversial. For better understanding a meta-analysis was therefore performed. Methods: An extensive search was performed to identify all case-control studies investigating association between SULT1A1 Arg213His polymorphism and lung cancer risk. The strength was assessed by odds ratio (OR) with the corresponding 95% confidence interval (95%CI). Results: A total of five publications covering 1,669 cases and 1,890 controls were included in this meta-analysis. No significant association between SULT1A1 Arg213His polymorphism and lung cancer risk was observed in overall comparisons in all genetic models (dominant model: OR=1.33, 95%CI=1.00-1.76, P=0.05; additive model: OR=1.30, 95%CI=0.93-1.81, P=0.12; recessive model: OR=1.21, 95%CI=0.89-1.66, P=0.23). However, on subgroup analysis, an elevated risk in mixed populations with variant His allele was revealed in the dominant model (OR=1.66, 95% CI=1.06-2.62, P=0.03). Furthermore, the SULT1A1 Arg213His polymorphism was associated with an increased risk of lung cancer in both females and males in the dominant model (females: OR=1.72, 95%CI=1.29-2.27, P=0.00; males: OR=1.46, 95%CI=1.19-1.78, P=0.00). No significant association between this polymorphism and different smoking status (smokers and non-smokers) and the other ethnicities (Asians and Caucasians) was shown. Conclusions: The results of this meta-analysis indicate that the SULT1A1 Arg213His polymorphism is not associated with lung cancer risk in Asians and Caucasians, but possible elevation for genotype (GA/AA) in mixed populations and males and females needs further investigation.

• Journal of the Korean Chemical Society
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• v.46 no.3
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• pp.213-218
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• 2002

(${\eta}$5-Cyclopentadienyl)(${\eta}$6-1,2,3,4-tetramethyl-1,4-dibora-2,5-cyclohexadiene)Ni (5) was synthe-sizedas a main product by the reaction of cyclopentadienyl(carbonyl)nickel dimer and 1,2,3,4-tetramethyl-1,4-di-bora-2-cyclohexene (1). Di-allyl nickel reacted with 2,3-diethyl-1,4-di-methyl-1,4-dibora-2-cyclohexene (2) at -20$^{\circ}C$ to give bis(${\eta}$6-2,3-diethyl- 1,4-dimethyl-1,4-dibora-2,5-cyclohexadiene)nickel (6) in the yield of 15%. By the reaction of di-allyl nickel and 2,3-dimethyl-1,4-diethoxy-1,4-di- bora-2-cyclohexene (3) at -20$^{\circ}C$ bis[${\eta}$6-2,3-dim-ethyl-1,4-diethoxy-1,4-di- bora-2,5-cyclohexadiene]nickel (7) was obtained in 22% yield. These double-decker complexes were difficult to separate because of their unstabilities and were identified by ESR, NMR, MS and ele-mental analysis, etc.

• Magazine of the Korea Concrete Institute
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• v.8 no.4
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• pp.161-169
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• 1996

Styrene-maleic anhydride copolymers with different composition (1:1SMA. 5:1SMA) were synthesized and further reacted with sulfuric acid to obtain water soluble copolymers (1:1SSMA, 5:1SSM.4). In the flow experiments of cement mortar mixed with copolymers, 5:1SSMA showed higher fluidity than 1:1SSMA. The cement mortar mixed with 1% 5:1SSMA maintained 95% of original flow after 60 min. On the other hand, the compressive strength of the hardened cement mortars containing 0.5% copolymers after 28 days curing was also examined. The compressive strength of hardened cement mortar containing 5:1SSMA was increased up to 41% compared to the plain while 1:1SSMA was increased up to 29%. As the results, the 5:1SSMA used in this study are greatly expected as a new high range water reducers for the concrete.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.8 no.5
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• pp.113-119
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• 2008

Conventional 1-1-1 cooperative protocol offers path-loss gain as advantage of multi-hop and spatial diversity which is equivalent to MIMO system. This protocol is enable to get higher reliability and reduction of power consumption than those of the single-hop or multi-hop. But the 1-1-1 cooperative protocol get only the diversity order 2 and limited path-loss reduction gain because this protocol has a single cooperative relay. We propose 1-2-1 cooperative protocol using two cooperative relays R1, R2. The 1-2-1 cooperative protocol can improve path-loss reduction and increase diversity order 3. Moreover, the cooperative relay R2 attains diversity order 2. The signaling method in transmission uses DF (Decode and Forward) or DR (Decode and Reencode) and 1-2-1 DF/DR cooperative protocol are applied to clustering based wireless sensor networks (WSNs). Simulations are performed to evaluate the performance of the protocols under Rayleigh fading channel plus AWGN (Additive White Gaussian Noise).

• Biomolecules & Therapeutics
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• v.16 no.4
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• pp.336-342
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• 2008

The resveratrol analogue piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene) is a polyphenol present in grapes and wine and reported to have anti-carcinogenic activities. To investigate the mechanism of anticarcinogenic activities of piceatannol, the effects on CYP 1 enzymes were determined in Escherichia coli membranes coexpressing recombinant human CYP1A1, CYP1A2 or CYP1B1 with human NADPH-P450 reductase. Piceatannol showed a strong inhibition of CYP1A1 and CYP1B1 in a concentration-dependent manner, and $IC_{50}$ of human CYP1A1 and CYP1B1 was 5.8 ${\mu}M$ and 16.6 ${\mu}M$, respectively. However, piceatannol did not inhibit CYP1A2 activity in the concentration of up to 100 ${\mu}M$. Piceatannol exhibited 3-fold selectivity for CYP1B1 over CYP1A1. The mode of inhibition of piceatannol was non-competitive for CYP1A1 and CYP1B1. The result that piceatannol did not inhibit CYP1B1-mediated $\alpha$-naphthoflavone ($\alpha$-NF) metabolism suggests piceatannol may act as a non-competitive inhibitor as well. In human prostate carcinoma PC-3 cells, piceatannol induces apoptosis and prevents Aktmediated signal pathway. Taken together, abilities of piceatannol to induce apoptotic cell death as well as CYP1 enzyme inhibition make this compound a useful tool for cancer chemoprevention.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.42-48
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• 2001

Allele rescue and sequence analysis of soo1-1 allele in Saccharomyces cerevisiae mutant LP0353 revealed that soo1-1 is identical to the previously reported ret1-1 allele, which has a base substitution of A for $G^{681}$ leading to an amino acid substitution of aspartic acid for $glycine^{227}$ in Soolp. However, it was revealed that the addition of osmotic stabilizer, such as 1.2M sorbitol can rescue the temperature sensitive phenotype of the ret1-1 mutant and that the soo1-1/ret1-1 mutation may confer defects in post-translational modification of proteins involved in the yeast cell wall biogenesis. Evidence for a putative role of 5th WD40 domain of the Soo1p/$\alpha$-COP in the construction and maintenance of cell walls was also presented by complementation test with deletion constructs of the SOOl.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.298-304
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• 2002

The inheritance of resistance to gall mite(Eriophys macrodonis) in boxthorn cultivars was studied with plants infected in open field. Segregation of the F$_1$ generation from crosses between resistant cultivars(Japan 1 and China 1) and susceptible line(CL42-56) hits a 3 resistant : 1 susceptible ratio, and the segregation ratio was 15 : 1 in cross between Japan 1 and China 1. It is concluded that gall mite resistance seemed to be controlled by two duplicate genes(El and E2), such that E1e1E2e2 symbolize the genotypes of China 1 and Japan 1. However, F, generation from Chungyanggugiza and CL42-56 cross did not showed clear segregation pattern but continuous variation was observed against gall mite resistance when resistance was determined on the basis of the percentage of galled leaves. Results indicated that the inheritance of resistance in Chungyanggugiza may be polygenic and complex. Also, Japan 1 and China 1 has a strong potential as a source of gall mite resistance, and Chungyanggugiza showing less resistance still has a valuable source.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.107-108
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• 2003

This study was conducted to investigate the effects of dilution rate and stored temperature of semen at 5, 25 and 35$^{\circ}C$ on fertility in liquid rooster semen. At 5$^{\circ}C$ cold temperature, no significant difference were found in sperm mobilities on dilution rate(1:1, 1:3, 1.6) among treatments. Sperm mobility for the conservation of 3 hours at 25∼35$^{\circ}C$ were significantly higher for 1:3 and 1:6 dilution rate(semen：diluent) groups than for 1:1 dilution rate group(P<0.05). In Fertility results after artificial insemination with the conservation of 3 hours at 5∼25$^{\circ}C$ temperature, no significant difference were found in fertility on dilution rate among treatments. Fertilities after artificial insemination with the conservation of 3 hours at 35$^{\circ}C$ were significantly higher for 1.3 and 1:6 dilution rate(semen：diluent) groups than for 1：1 dilution rate group(P<0.05).

• Korean Journal of Occupational Health Nursing
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• v.32 no.2
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• pp.79-88
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• 2023

Purpose: This study aims to identify the factors that influence the experience of falls among older adults living in the community. Methods: The study participants were 70,887 65-year-olds who participated in the 2021 Community Health Survey. The study employed the Rao-scott x 2 test to examine the variation in fall experiences based on the characteristics of the older adults. Multiple logistic regression analysis was conducted to investigate these characteristics' impact on older adults' fall experiences. Results: The proportion of subjects in fall experience was 16.6%. The factors influencing the subject's fall experience were sex (odds ratio [OR]=1.47, 95% confidence interval [CI]=1.37~1.57), age (OR=1.48, 95% CI=1.34~1.65), family structure (OR=1.23, 95% CI=1.15~1.31), body mass index (OR=1.13, 95% CI=1.06~1.20), diabetes (OR=1.12, 95% CI=1.06~1.20), depression experiences (OR=1.56, 95% CI=1.42~1.70), stress (OR=1.12, 95% CI=1.05~1.19), subjective health status (OR=1.77, 95% CI=1.63~1.92), life satisfaction (OR=1.57, 95% CI=1.41~1.76), and chewing discomfort (OR=1.29, 95% CI=1.21~1.38). Conclusion: Efforts should be made to effectively educate and develop various programs aimed at reducing falls among older adults. It is essential to emphasize the importance of continuous and active attention to falls in the older adult population.