• 한국버섯학회지
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• 제21권3호
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• pp.145-149
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• 2023

일부 버섯품목의 편중재배 해소를 목적으로 경쟁력이 있고 상품성 있는 백령느타리를 육성하고자 하였다. 국내 외에서 유전자원을 수집하고 교배하여, 특성검정 및 생산력 검정, 농가실증의 과정을 통해 육성된 백령느타리 신품종 '보람'의 주요 특성은 다음과 같다. 균사생장적온은 26~29℃, 발이 및 생육온도는 15~18℃이고, 병재배 기준으로 재배일수는 95일로 대조품종(우람)과 유사하였고, 형태는 둥근형으로 주걱형인 대조품종(우람)과 구별되었다. 병재배에서 자실체 갓의 직경과 두께, 대굵기는 각각 73.5 mm, 15.7 mm, 37.0 mm로 대조품종에 비해 갓 직경이 작지만 갓 두께와 대 굵기는 두꺼웠다. 병당 수량은 1,100 cc(∅ 75 mm)병 기준 181.1 g으로 대조품종(180.4 g)과 통계적으로 유의한 차이가 없었다. 경기도 여주 소재 병재배, 봉지재배 농가에서 실증한 결과, 재배방법과 배지량의 차이가 자실체 크기와 형태, 수량에 영향을 미치는 것으로 추정되었다. 신품종 '보람'을 대상으로 대치배양 하였을 때 대치선이 뚜렷하고, 균사체의 PCR 증폭 결과 밴드패턴이 모본 및 대조품종과 다른 양상을 보여 교배종임을 확인하였다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.10-10
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• 2023

본질적으로 복잡하고 다양한 특성을 가지는 우리나라(도시, 농어촌, 도서산간, 섬 등)의 물 공급 시스템은 생활수준의 향상, 기후변화 및 가뭄위기, 소비환경 중심의 요구와 한정된 수자원을 잘 활용하기 위한 운영 및 관리가 매우 복잡하다. 이로 인한 수자원 고갈과 가뭄위기 등에 관련한 대책 및 방안으로 대체수자원인 지하수 활용방안들이 제시되고 있다. 따라서, 물 관리 시스템과 관련한 디지털 기술은 오늘날 플랫폼과 디지털 트윈의 도입을 통해 네트워크와 가상현실 세계의 연결이 통합되어진 4차 산업혁명 사업이 현실화되고 있다. 물 관리 시스템에 사용된 새로운 디지털 기술 "BDA(Big Data Analytics), CPS(Cyber Physical System), IoT(Internet of Things), CC(Cloud Computing), AI(Artificial Intelligence)" 등의 성장이 증가함에 따라 가뭄대응 위기와 도시 지하수 물 순환 시스템 운영이 증가하는 소비자 중심의 수요를 충족시키기 위해서는 지속가능한 지하수 공급을 효과적으로 관리되어야 한다. 4차 산업혁명과 관련한 기술성장이 증가함으로 인한 물 부문은 시스템의 지속가능성을 향상시키기 위해 전체 디지털화 단계로 이동하고 있다. 이러한 디지털 전환의 핵심은 데이터에 관한 것이며, 이를 활용하여 가치 창출을 위해서 "Digital Groundwater Technology/Twin(DGT)"를 극대화하는 방식으로 제고해야 한다. 현재 당면하고 있는 기후위기에 따른 가뭄, 홍수, 녹조, 탁수, 대체수자원 등의 수자원 재해에 대한 다양한 대응 방안과 수자원 확보 기술이 논의되고 있다. 이에 따른 "물 순환 시스템"의 이해와 함께 문제해결 방안도출을 위하여 이번 "기획 세션"에서는 지하수 수량 및 수질, 정수, 모니터링, 모델링, 운영/관리 등의 수자원 데이터의 플랫폼 동시성 구축으로부터 역동적인 "DGT"을 통한 디지털 트윈화하여, 지표수-토양-지하수 분야의 특화된 연직 프로파일링 관측기술을 다각도로 모색하고자 한다. "Digital Groundwater(DG)"는 지하수의 물 순환, 수량 및 수질 관리, 지표수-지하수 순환 및 모니터링, 지하수 예측 모델링 통합연계를 위해 지하수 플랫폼 동시성, ChatGPT, CPS 및 DT 등의 복합 디지털화 단계로 나가고 있다. 복잡한 지하환경의 이해와 관리 및 보존을 위한 지하수 네트워크에서 수량과 수질 데이터를 수집하기 위한 스마트 지하수 관측기술 개발은 큰 도전이다. 스마트 지하수 관측기술은 BD분석, AI 및 클라우드 컴퓨팅 등의 디지털 기술에 필요한 획득된 데이터 분석에 사용되는 알고리즘의 복잡성과 데이터 품질에 따라 영향을 미칠 수 있기 때문이다. "DG"는 지하수의 정보화 및 네트워크 운영관리 자동화, 지능화 등을 위한 디지털 도구를 활용함으로써 지표수-토양층-지하수 네트워크 통합관리에 대한 비전을 만들 수 있다. 또한, DGT는 지하수 관측센서의 1차원 데이터 융합을 이용한 지하수 플랫폼 동시성과 디지털 트윈을 연계할 수 있다.

• International journal of advanced smart convergence
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• 제11권4호
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• pp.227-239
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• 2022

Purpose: In our study, in order to find lactic acid bacteria (LAB) with multi-drug resistance to antibiotics, we isolated 140 strains from 15 types of kimchi commercially available in Korea and 20 types of Kimchi made at home from January to December in 2016, and investigated their H₂O₂ generating ability and multi-drug resistance to antibiotics. Methods: In order to observe the H₂O₂ generation ability of LAB, we performed the experiment with methods such as Rabe, Hillier, and Kang. To test the antibacterial susceptibility of LAB, we used the disc agar diffusion method using MRS agar (Difco, USA) according to the CLSI and WHO test methods. There are 18 types of antibiotic discs used. Results: Out of the total numbers of 140 strains, 6 strains of Ent. Faecium, 25 strains of L. plantarum, 1 strain of L. rhamnosus, 3 strains of L. sakei, 1 strain of L. acidophilus, 1 strains St. thermophilus, and 7 of unidentified strains generated H₂O₂. The antibiotic susceptibility of Ent. Faecium indicated SXT, OX, NA, and E; and the antibiotic susceptibility of L. plantarum indicated NA; and the antibiotic susceptibility of St. thermophilus indicated NA, CC, RA, CTT, CM, and P ; and the antibiotic susceptibility of L. rhamnosus indicated SXT, VA, NA and CTT; and the antibiotic susceptibility of 6 strains of L. sakei indicated SXT, OX, NOR, NA, CTT and CIP, all indicating antibiotic resistance. In the case of multi-drug resistance to antibiotics for 53 strains of L. antarum, 8-drug resistance was the most common with 25 strains, followed by 7-drug-resistant strains with 18 strains, 9-drug-resistant strains with 4 strains, 6-drug-resistant strains with 3 strains, 5-drug-resistant strains with 2 strains, and 17-drug-resistant strains with 1 strain. In the case of multi-drug resistance to antibiotics for Ent. Faecium 27 strains, 9-drug resistance was most commonly identified as 9 strains, 8-drug resistance was identified as 6 strains, 7- and 11 drug resistances were identified as 4 strains each, and 4- and 6-drug resistances were identified as 1 strain each. Conclusion: Ent. Faecium, L. plantarum, L. rhamnosus, L. sakei, and St. thermophilus, shown to have anantibacterial activity in previous studies on LAB and shown to have and H₂O₂ generating ability, antibiotic resistance and multi-drug resistance in this study, are expected to be able to play an excellent role for long-term inpatients to use as an alternative to antibiotics and to cope with emerging antibiotic resistance.

• 분석과학
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• 제22권1호
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• pp.44-50
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• 2009

본 연구에서는 퍼지 & 트랩 GC-MS를 이용한 퇴적물 중의 메틸수은 분석방법을 제시하고, 국내 주요 하천 퇴적물 중의 총수은 및 메틸수은 등을 분석하였다. 분석방법의 검증을 위하여 고농도 및 저농도의 인증표준물질 2종을 분석하였으며, 현장시료 81건에 대하여 EPA 1630 method 원리의 GCCVAFS 방법으로 분석한 결과와 GC-MS 방법의 분석결과를 비교하였다. 방법검출한계 (MDL)는 0.06 ng/g, 정량한계 (MQL)는 0.20 ng/g이었으며 정밀도와 정확도는 각각 11.2% 및 $102{\pm}11.4%$로 나타났다. 조사된 81건에 대한 하천 퇴적물의 총수은 및 메틸수은 농도는 $2.43{\sim}1.56{\times}10^3$ ng/g 및 N.D.~5.95 ng/g 로 나타났다. 총수은의 분포특성은 외부오염원에 의한 영향으로 인해 유기물, 황함량 및 납, 크롬, 니켈 등의 중금속 농도와 유의한 상관성이 나타났으나, 메틸수은의 경우, 총수은을 제외한 다른 인자들과의 유의한 상관성이 조사되지 않았다.

• Animal Bioscience
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• 제35권10호
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• pp.1489-1498
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• 2022

Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep. Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population. Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes. Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.114-114
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• 2022

There are 28 families and 166 species of exotic weeds on agricultural land and among these, 23 families and 80 species of exotic weeds occur on pastures. Among them, the Solidago altissima is a perennial weed belonging to the asteraceae family and it is an exotic weed that spreads to the surrounding area using methods such as high seed production, vegetative propagation using underground rhizomes and allelochemical. Accordingly, in 2009, the Ministry of Environment designated it as an ecosystem-disrupting species. This study was conducted to obtain basic data about the effects of S.altissima derived allelochemicals on forage crops. The root of S.altissima was separated, dried in the shade and then pulverized to prepare an root powder. Powder was repeatedly extracted with methanol for 3 days and concentrated under reduced pressure to obtain an root methanol extract. Dissolve the extract in distilled water, dispense it in a separate-funnel and proceed with liquid-liquid extraction by adding equal amounts of n-haxane (Hex), chloroform (CHCI₃), ethyl acetate (EtoAC), and butanol (BuOH) in order of increasing polarity. A seed-bioassay was performed using fractions for each solvent, followed by separation and purification by silica gel column chromatography. As a result of the fraction germination test for each solvent, the IC₅₀ values using the fresh weight of each fraction were 898.3 mg L^-1, 676.3 mg L^-1, 1160 mg L^-1 and 1360 mg L^-1. CA, CB, and CC fractions were obtained through primary silica gel column chromatography that used CHCI₃ fraction. As a result of seed-bioassay using each fraction, the IC₅₀ values for the fresh weight of each fraction was 537.3 mg L^-1, 1280 mg L^-1 and 1947 mg L^-1. Based on this, 5 fractions were obtained as a result of secondary silica gel column chromatography using the CA fraction. A seed-bioassay was performed, as a result, the lowest IC₅₀ value was calculated as 226.7 mg L^-1 in the CAE fraction. Based on this, the fraction was analyzed by GC-MS. The results of this study can be used as basic research data on the effects of weeds on forage crops and allelochemicals secreted from S. altissima.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.36-36
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• 2022

The molecular understanding of resistance and susceptibility of host plants to scab, a most threatful disease to pome fruit production worldwide, is very limited. Comparing resistant line '93-3-98' to susceptible one 'Sweet Skin' at seven time points of 0, 0.5, 1, 2, 3, 4, 8 days post inoculation, RNA-sequencing data derived from infected and mock-inoculated young leaves were analyzed to evaluate the tolerant response and to mine candidate genes of pear to the scab pathogen Venturia nashicola. Analysis of the mapped reads showed that the infection of V. nashicola led to significant differential expression of 17,827 transcripts with more than 3-fold change in the seven pairs of libraries, of which 9,672 (54%) are up- and 8,155(46%) are down-regulated. These included mainly receptor (NB-ARC domains-containing, CC-NBS-LRR, TIR-NBS-LRR, seven transmembrane MLO family protein) and transcription factor (ethylene responsive element binding, WRKY DNA-binding protein) related gene. An arsenal of defense response of highly resistant pear accessions derived from European pear was probably supposed no sooner had V. nashicola infected its host than host genes related to disease suppression like Polyketide cyclase/dehydrase and lipid transport protein, WRKY family transcription factor, lectin protein kinase, cystein-rich RLK, calcium-dependent phospholipid-binding copine protein were greatly boosted and eradicated cascade reaction induced by pathogen within 24 hours. To identify transcripts specifically expressed in response to V. nashicola, RT-PCRs were conducted and compare to the expression patterns of seven cultivars with a range of highly resistant to highly susceptible symptom. A DEG belonging to the PR protein family genes that were higher expressed in response to V. nashicola suggesting extraordinary role in the resistance response were led to the identification. This study provides the first transcriptional profile by RNA-seq of the host plant during scab disease and insights into the response of tolerant pear plants to V. nashicola.

• Nuclear Engineering and Technology
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• 제55권10호
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• pp.3543-3548
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• 2023

Shutdown chemistry evolution is performed in nuclear power plants at each refueling outage (RFO) to establish safe conditions to open system and minimize inventory of corrosion products in the reactor coolant system (RCS). After hydrogen peroxide is added to RCS during shutdown chemistry evolution, corrosion products are released and are removed by filters and ion exchange resins in the chemical volume control system (CVCS). Shutdown chemistry evolution including RCS clean-up time to remove released corrosion products impacts the critical path schedule during RFOs. The estimation of clean-up time prior to RFO can provide more reliable actions for RCS clean-up operations and transients to operators during shutdown chemistry. Electric Power Research Institute (EPRI) shutdown calculator (SDC) enables to provide clean-up time by Co-58 peak activity through operational data from nuclear power plants (NPPs). In this study, we have investigated the results of EPRI SDC by shutdown chemistry data of Co-58 activity using NPP data from previous cycles and modeled the estimated clean-up time by EPRI SDC using average Co-58 activity of the NPP. We selected two RFO data from the NPP to evaluate EPRI SDC results using the purification time to reach to 1.3 mCi/cc of Co-58 after hydrogen peroxide addition. Comparing two RFO data, the similar purification time between actual and computed data by EPRI SDC, 0.92 and 1.74 h respectively, was observed with the deviation of 3.7-7.2%. As the modeling the estimated clean-up time, we calculated average Co-58 peak concentration for normal cycles after cycle 10 and applied two-sigma (2σ, 95.4%) for predicted Co-58 peak concentration as upper and lower values compared to the average data. For the verification of modeling, shutdown chemistry data for RFO 17 was used. Predicted RCS clean-up time with lower and upper values was between 21.05 and 27.58 h, and clean-up time for RFO 17 was 24.75 h, within the predicted time band. Therefore, our calculated modeling band was validated. This approach can be identified that the advantage of the modeling for clean-up time with SDC is that the primary prediction of shutdown chemistry plans can be performed more reliably during shutdown chemistry. This research can contribute to improving the efficiency and safety of shutdown chemistry evolution in nuclear power plants.

• Tuberculosis and Respiratory Diseases
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• 제87권1호
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• pp.65-79
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• 2024

Background: Exhaled condensates contain inflammatory biomarkers; however, their roles in the clinical field have been under-investigated. Methods: We prospectively enrolled subjects admitted to pulmonology clinics. We collected exhaled breath condensates (EBC) and analysed the levels of six and 12 biomarkers using conventional and multiplex enzyme-linked immunosorbent assay, respectively. Results: Among the 123 subjects, healthy controls constituted the largest group (81 participants; 65.9%), followed by the preserved ratio impaired spirometry group (21 patients; 17.1%) and the chronic obstructive pulmonary disease (COPD) group (21 patients; 17.1%). In COPD patients, platelet derived growth factor-AA exhibited strong positive correlations with COPD assessment test (ρ=0.5926, p=0.0423) and COPD-specific version of St. George's Respiratory Questionnaire (SGRQ-C) score (total, ρ=0.6725, p=0.0166; activity, ρ=0.7176, p=0.0086; and impacts, ρ=0.6151, p=0.0333). Granzyme B showed strong positive correlations with SGRQ-C score (symptoms, ρ=0.6078, p=0.0360; and impacts, ρ=0.6007, p=0.0389). Interleukin 6 exhibited a strong positive correlation with SGRQ-C score (activity, ρ=0.4671, p=0.0378). The absolute serum eosinophil and basophil counts showed positive correlations with pro-collagen I alpha 1 (ρ=0.6735, p=0.0164 and ρ=0.6295, p=0.0283, respectively). In healthy subjects, forced expiratory volume in 1 second (FEV₁)/forced vital capacity demonstrated significant correlation with CC chemokine ligand 3 (CCL3)/macrophage inflammatory protein 1 alpha (ρ=0.3897 and p=0.0068). FEV₁ exhibited significant correlation with CCL11/eotaxin (ρ=0.4445 and p=0.0017). Conclusion: Inflammatory biomarkers in EBC might be useful to predict quality of life concerning respiratory symptoms and serologic markers. Further studies are needed.

• Journal of Cardiovascular Imaging
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• 제30권3호
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• pp.153-168
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• 2022

Recent studies have shown that sodium/glucose cotransporter 2 (SGLT2) inhibitors might exert favourable changes on cardiac parameters as observed on cardiovascular imaging. We conducted a systematic review and meta-analysis to determine the effects of SGLT2 inhibitors on cardiac imaging parameters. Four electronic databases (PubMed, Embase, Cochrane, Scopus) were searched for studies in which the effects of SGLT2 inhibitors on cardiac imaging parameters were examined. Studies in which a population was administered SGLT2 inhibitors and analysed by echocardiography and/or cardiac magnetic resonance (CMR) imaging were included. Random-effects pair-wise meta-analysis models were utilized to summarize the studies. A total of 11 randomized controlled trials was included with a combined cohort of 910 patients. Comparing patients receiving SGLT2 inhibitors with subjects receiving placebo, the mean change in CMR-measured left ventricular mass (LVM) was -3.87 g (95% confidence interval [CI], -7.77 to 0.04), that in left ventricular end-systolic volume (LVESV) was -5.96 mL (95% CI, -10.52 to -1.41) for combined LVESV outcomes, that in left atrial volume index (LAVi) was -1.78 mL/m² (95% CI, -3.01 to -0.55) for combined LAVi outcomes, and that in echocardiography-measured E/e' was -0.73 (95% CI, -1.43 to -0.03). Between-group differences were not observed in LVM and LVESV after indexation. The only between-group difference that persisted was for LAVi. Treatment with SGLT2 inhibitors resulted in reduction in LAVi and E/e' on imaging, indicating they might have an effect on outcomes associated with LV diastolic function.