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Isolation of Polyphenol from Green Tea by HPLC and Its Physiological Activities (HPLC에 의한 녹차의 polyphenol 화합물의 분리 및 polyphenol의 생리활성)

  • Woo, Hee-Seob;Choi, Hee-Jin;Han, Ho-Suk;Park, Jung-Hye;Son, Jun-Ho;An, Bong-Jeun;Son, Gyu-Mok;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1199-1203
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    • 2003
  • Polyphenols were isolated from Korean green tea using Sephadex LH-20 and HPLC. The isolated polyphenols were procyanidin B-4, procyanidin B-2-3,3'-digallate, prodelphinidin C-2-3,3'-di-O-digallate, (+)-catechin-3-O-rhamnose, procyandin B-5, procyanidin B-7-3-0-gallate, gallate, epiafzelechin-$(4{\beta}{\rightarrow}8)$-epiafzelechin, procyanidin B-3-3-O-rhamnose, afzelechin-$(4{\alpha}{\rightarrow}8)$-catechin, prodelphinidin B-5-3,3'-di-O-digallate and (+)-taxifolin-3-O-D-xyloside. The inhibitory effects of prodelphinidin C-2-3,3'-di-O-gallate and procyanidin B-2-3,3'-digallate $(at\;100{\mu}M)$ on angiotensin.converting enzyme were 68.8 and 54.6%, respectively, while the inhibitory effects of prodelphinidin C-2-3,3'-di-O-gallated and procyanidin B-2-3,3'-digallate $(at\;100{\mu}m)$ on xanthine oxidase were 54.5 and 38.2%, respectively. Lastly, the inhibitory activities of prodelphinidin C-2-3,3'-di-O-gallate $(at\;100{\mu}m)$ on tyrosinase was 42.1%.

A study on dental health and physical & psychological health status of the aged (노인 구강보건실태 및 신체·심리적 건강상태에 관한 연구)

  • Yoon, Young-Suk;Jung, Young-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.5 no.1
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    • pp.39-51
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    • 2005
  • This study was conducted to investigate the dental health and physical & psychological health status of the aged. The subjects were 61 old persons, women who were over 65, living at Sahagu in Pusan. The data for this study were collected by using direct interviewing method from October 22th, 2004. The data were analysed by using a computerized program named statistical package for social science including frequency, percentage, ANOVA. The results of the study were summarized as follows: 1. The dental status and the use of dental care institute 1) In status of denture use, it was found to be no denture(45.2%), full denture(35.5%), partial denture(19.4%). 2) In the questionaire of "when did you go to the dental care institute recently?", it was found to be uncertain(41.9%), less than one year(38.8%), 2~3years(16.1%), 1~2years(3.2%). 3) In the questionaire of "what type of the dental care institute did you use?", it was found to be in the dental clinic(80.6%), others(9.7%), dental hospital(6.5%), department of dentistry in general hospital(3.2%). 4) In the questionaire of "why did you select the dental care institute?", it was found to be in the nearly distance(80.6%), relative advice(16.1%), advertisement(3.2%). 5) In the questionaire of "how did you go to the dental care institute?", it was found to be walking(71.0%), bus & subway(22.6%), car(3.2%) and others(3.2%). 6) In the questionaire of "how long did it take to the dental care institute?", it was found to be 10~30min(48.4%), less than 10min(38.7%), 30min~1h(6.5%), 1~2h(3.2%), more than 2h(3.2%). 7) In the questionaire of "what kind of dental care did you take?", it was found to be denture making(45.2%), extraction of tooth, dental surgery, general exam(12.9%), caries(9.7%), denture repair(6.5%), others(3.2%). 8) In the questionaire of "how much did you pay for dental care recently?", it was found to be less than 5,000won(35.5%), 20,000~100,000won(19.4%), 1~3million won(16.1%), 5,000~20,000won, 100,000~300,000won, 500,000~1million won, more than 3million(6.5%), 300,000~500,000won(3.2%). 9) Average score of the subject's physical health status was 4.11 and psychological health status, 4.01 in a 5 point Likert scale. 10) The physical and psychological health status showed the significant differences according to the frequency of eating snack(pE0.05) and snack type(pE0.01). Above findings suggest that geriatric oral health program is necessary in improving the dental health & health status of the aged.

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Knowledge and attitude change towards radiation protection after radiation safety management education in dental hygiene students (치위생과 학생들의 학업관련 특성에 따른 방사선안전관리 교육 후 방사선 방어에 대한 지식과 태도의 변화)

  • Kim, Sung-Ae;Lee, Ji-Young;Hwang, Se-Hyun;Cho, Mi-Suk;Park, Jung-Hyun;Jung, Nam-Yeong;Yu, Byeng-Chul
    • Journal of Korean society of Dental Hygiene
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    • v.15 no.1
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    • pp.101-109
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    • 2015
  • Objectives: The purpose of the study is to investigate the knowledge and attitude towards radiation protection after radiation safety management education in dental hygiene students. Methods: After receiving informed consents, a self-reported questionnaire was carried out for 135 dental hygiene students in Busan on June 17 for preliminary survey and September 3 for post-education survey, 2011. The questionnaire was modified from Han and consisted of 5 questions of general characteristics, 6 questions of radiation use, 7 questions of scholastic characteristics, 15 questions of knowledge towards radiation safety management, and 15 questions of attitude towards radiation safety management. Cronbach's alpha was 0.808 in the knowledge towards radiation safety management. Attitude towards radiation safety management included 15 questions of general attitude towards radiation safety management, individual and patient, and attitude toward radiation exposure reduction measured by Likert 5 scale. Cronbach's alpha was 0.970 in the attitude towards radiation safety management. Data were analyzed using SAS version 9.2 for educational analysis, technical analysis and multivariate analysis, paired t-test, and GLM. Results: Significant differences were shown in the knowledge and attitudes towards radiation safety management; the level of knowledge was respectively $9.8{\pm}3.0$ and $12.9{\pm}1.9$ points before and after education, and the level of attitude was $4.28{\pm}0.51$ and $4.53{\pm}0.47$ before and after the education. The levels of knowledge and attitude according to general characteristics showed a significant differences in grade and academic results. Post-education survey suggested that the education is very important and necessary and can change the knowledge and attitude towards radiation safety management in the dental hygiene students. Conclusions: Through the radiation safety management education, the levels of knowledge and attitude towards radiation safety management have changed significantly, which showed that the radiation safety management education proved to be very effective. Therefore, an effective radiation safety management education program is necessary to improve the levels of knowledge and attitude towards radiation safety management in the dental hygiene students.

Physicochemical Properties of Onion Powder Added Wheat Flour Dough (양파분말을 첨가한 빵반죽의 물리화학적 특성)

  • Bae, Jong-Ho;Woo, Hi-Seob;Choi, Hee-Jin;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.436-441
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    • 2003
  • The physiochemical properties of wheat flour dough added with onion powder were investigated. The color value of mixed onion powder was added and had low values of lightness and redness, as well as high value of yellowness. Water absorption of the wheat flour dough decreased with the increase in the onion powder. Both development time and stability of the wheat flour dough with onion powder added were less than those of the control. An increase in the added amount of onion powder resulted in an increase of weakness. The maximum viscosity gradually decreased with the increase the amount of onion powder, while the temperature of gelatination did not change. The wheat flour dough extensibility decreased, and the resistance of extension and area under the curve increased in the onion powder. It is of considerable note that the pH of the wheat flour dough decreased in the process of fermentation with an increase in the amount of onion powder.

Beauty activity of extracts from Saurus chinensis by ultra-fine ground (초미세 분쇄한 삼백초(Saurus chinensis) 추출물의 미용 활성)

  • Park, Hye-Jin;Park, Ki-Tae;Hong, Shin-Hyub;Kim, Na-Hyun;Park, Mi-Jeong;Hong, Eun-Jin;Ahn, Dong-Hyun;An, Bong-Jeun;Cho, Young-Je
    • Food Science and Preservation
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    • v.23 no.6
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    • pp.899-905
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    • 2016
  • In this study, beauty food activities were determined for extracts of Saurus chinensis as functional beauty food. Tyrosinase inhibitory effect was increased as more 4 folds by ultra-fine grind technology than normal grind technology in ethanol extracts from Saurus chinensis. A ultra-fine ground technology was used to increase the extraction yield for bioactive compounds from medicinal plants. Tyrosinase inhibitory activity, which is related to skin-whitening, was confirmed to be 100% for ethanol extracts at 1,000 ppm of phenolics. Anti-wrinkle activity was determined showing 30% of inhibition effect at above 1,000 ppm phenolic concentration. The elastase inhibitory activity of water and ethanol extracts from ultra-fine ground Saurus chinensis showed higher inhibition activity than compared to extracts from normal ground Saurus chinensis. Astringent activity in ethanol extracts was determined showing over 70% at 250 ppm of phenolics. Based on these results, Saurus chinensis extracts can be used as a functional beauty food and cosmetic with whitening, anti-wrinkle and pore contraction effects.

Hyaluronidase inhibitory activity of extracted phenolic compounds from ultrafine grind Saururus chinensis (초미세 분쇄한 삼백초로부터 추출된 phenolic 화합물의 hyaluronidase 억제 효과)

  • Park, Ki-Tae;Oh, Sang-Lyong;Cho, Young-Je
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.20-26
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    • 2016
  • In this study, the anti-inflammatory effect of hyaluronidase (HAase) inhibition was determined from 92 species of oriental herbal medicine extracted with water and ethanol solvents because of their non-toxicity in the human body. The water extracts of Evodia officinalis (86.8%), Thuja orientalis (80.8%), Carthami semen (66.5%), Melia azedarach (74.7%), Siegesbeckia pubescens (61.3%), Saururus chinensis (49.15%) showed a relatively greater anti-inflammatory activity. The ethanol extracts of Ailanthus altissima and Saururus chinensis demonstrated the highest anti-inflammatory effect at above 90%. Saururus chinensis was selected for its high anti-inflammatory effect in both water and ethanol extract. Ethanol was more effective than water and optimal extraction conditions for phenolic compounds was determined to be extraction with 50% ethanol for 12 hours. The extracts from Saururus chinensis in optimal condition showed 70~80% anti-inflammatory effect when $100{\sim}250{\mu}g/mL$ phenolic concentration was treated. Concentration of above $500{\mu}g/mL$ decreased the inhibitory effect. The anti-inflammatory effect and extraction yield were increased by ultra-fine grind technology, indicating that this method can be used to increase the extraction yield of phenolic compounds from medicinal plants.

A Study on Architecture and Urban Regeneration in Korea through the Perception of Body (몸의 지각론에 의한 유휴시설의 건축도시 재생에 관한 연구)

  • Hyung, Hyung-Chir;Joh, Hahn
    • Korean Institute of Interior Design Journal
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    • v.26 no.6
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    • pp.210-221
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    • 2017
  • First, we can define how our body perceives the external world and embodies its senses through the philosopher Merleau - Ponty. These philosophical orientations of Merleau-Ponty also appear to urban theorists such as Jane Jacobs, Gordon Cullen, and Juhani Pallasmaa. In other words, after the Second World War, people began to pay attention to human emotions and perceptions while opposing human rational thinking. Especially, they reject the abstract space of modernism and explore the everyday city space where the local character of the area lives. This place is a space where the collective memory of the group is shared over several generations. So, in this space, people's active perceptual system works actively. In the sense of this continuity of time, their ideas intersect with the concept of urban. Specifically, Jacobs criticizes massive development and proposes the development of a small block-based city with a commonality of old and new. In addition, we argue that urban space can be a visually interesting object through the continuous visual concept of urban theorist Cullen. In particular, he rediscovers the value of traditional urban space through visual experience between architecture and urban facilities. Finally, the architectural city theorist, Pallasmaa., criticizes the visual centrality of modern cities and thinks about the value of multidisciplinary space that can be experienced in architecture. This study examines the space of reproduction in detail on the perspective of the body philosophy and urban theorists. In other words, the play space inherits the natural city time, so when our body experiences this play space, we can actively sense and perceive the various senses. So we can invoke the active external actions of our bodies. Through the analysis of the size of the reconstruction space of the architectural city, various types of body senses and responses can be. Yoon Dongju Literary Museum, which renovated the old water tank of the city, can recognize the unfamiliar sense of body in everyday life through the traces and smells of water in the past and the restrained visuality. In addition, Seonyudo Park, which regenerates the waste water purification plant, can experience a phenomenal phenomenon through water space, old concrete and traces of steel. Finally, with the most recently played Seoul Road 7017 can experience interesting urban spaces in terms of a variety of plants, a human scale space creating movement, and a continuous visual.

Design and manufacture of mini loop coil probe style magnetic curer for peripheral nervous system treatment (말초신경계 치료를 위한 초미니 Loop-코일프로브 삽입형 자기치료기의 설계 및 제작)

  • Kim, Whi-Young;Choi, Jin-Young;Park, Sung-Jun;Kim, Hee-Je
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.2 no.1
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    • pp.153-169
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    • 2007
  • It is as result that study to apply mini magnet nerve curer in peripheral nervous system disease treatment. Design and embodied action power and Control Unit in cylinder form of magnet roof object firstly. Yielded service area about special quality of probe of roof object cylinder style and treatment area dimension and distance of treatment pulse secondly. Embody pulse forming course energy value by Probe's form by third, could embody treatment pulse by disease. Specially, through a special quality experiment, saved Damping pulse form and treatment pulse form etc. variously. Lately, embodied this to aid a little in disease treatment that follow that there is no invasion that there is no stimulation by medicine development. Go amplitude treatment pulse (traditional magneto-therapy of greatly great that strong) does curative effect greatest at short time and becomes thought that demand is magnified greatly at the future in this research.

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Characteristics of Insoluble Phosphates Solubilizing by Klebsiella sp. DA71-1/pLYJ (Klebsiella sp. DA71-1/pLYJ의 난용성 인산염 가용화 특성)

  • Ryu, Ah-Reum;Lee, Jin-Woo;Lee, Yong-Seok;Lee, Sang-Cheol;Chung, Soo-Yeol;Choi, Yong-Lark
    • Journal of Life Science
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    • v.16 no.4
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    • pp.676-682
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    • 2006
  • To develop high efficiency biofertilizer solubilizing insoluble phosphates, lactate dehydrogenase (ldh) gene was isolated from Staphylococcus sp. LJ2. Genetic constructions were carried out using the pGEM-T-easy vector and pHSG398. Recombinant DNA plasmids containing the ldh gene were transferred to Klebsiella sp. DA71-1 by electroporation. The selected transformant was named as a DA71-1/pLYJ. The insoluble phosphates solubilization activity of DA71-1/pLYJ was higher than that of DA71-1 at various culture conditions. Glucose was the best carbon source for insoluble phosphates solubilization among the used carbon sources. Maximal insoluble phosphates solubilizing was found in sucrose minimal (SM) medium containing 3% glucose. The solubilizing activity of DA71-1/pLYJ against three types of insoluble phosphates, such as tri-calcium phosphate, hydroxyapatite, aluminium phosphate, were quantitatively determined. The optimal temperature and initial pH to solubilize insoluble phosphates in the SM medium was $37^{\circ}C$ and pH 5.0, respectively.

A Change of the ability of CPR After educations for Some Health College Students and the factors Effects CPR Performance (일부 보건계열 대학생들의 심폐소생술 교육 전·후 비교 및 교육 후 수행능력에 미치는 영향)

  • Park, Sang-Sub;Park, Dae-Sung;Ko, Chong-Hyeon;Kim, Yeong-Ah;Park, Jae-Seong
    • The Korean Journal of Emergency Medical Services
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    • v.12 no.3
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    • pp.17-26
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    • 2008
  • Purpose : This study aimed to make comparison before and after CPR training for health college students (departments of visual optics, occupational therapy, and health welfare administration). The questionnaire consisted of self-efficacy, knowledge, and attitudes toward CPR affect performance ability. Through this study, the researchers tried to provide basic data in developing of cardiopulmonary resuscitation training programs and training methods for health college students. Methods : This study was applied to 83 health college students (departments of visual optics, occupational therapy, and health welfare administration) in G Province. Data were collected from September 22 to October 9, 2008. Analysis was performed by using SPSS WIN 12.0 Version program. Frequency analysis, t-test, ANOVA, and Pearson's correlation coefficients were used. Statistical significance was based on p < .05. Results : 1. There was an increase with(${\rightarrow}$ in) 1.69 point before education and 3.01 after education of artificial ventilation(p < .001). There was an increase in 1.46 point before education and 3.24 after education of airway(p < .001). There was an increase in 1.54 point before education and 2.84 after education of chest compression location(p < .001). There was an increase in 1.97 point before education and 3.13 after education of chest compression(p < .001). 2. The difference between self-efficacy, knowledge, and attitude before and after CPR education, the self-efficacy increased from 2.08 point before education(p < .001 to 3.18 point after education. Knowledge increased from 2.09 point before education(p < .001) to 3.28 point after education. Attitude increased from 1.75 point before education(p < .001 to 3.05 point after education. 3. The correlation between self-efficacy, knowledge, and attitude toward CPR), was not changed before education. After education, knowledge showed quantitative correlation(r = .219, p < .05) with attitude. 4. In regression analysis with the aim of examining influence of self-efficacy, knowledge, and attitude upon performance ability, the effect wasn't given to performance ability before education. After education, the self-efficacy was indicated to have significant effect on performance ability(p < .05).

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