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Screening and Identification of a Cesium-tolerant Strain of Bacteria for Cesium Biosorption (환경유래의 세슘 저항성 균주 선별 및 세슘 흡착제거 연구)

  • Kim, Gi Yong;Jang, Sung-Chan;Song, Young Ho;Lee, Chang-Soo;Huh, Yun Suk;Roh, Changhyun
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.304-313
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    • 2016
  • One of the issues currently facing nuclear power plants is how to store spent nuclear waste materials which are contaminated with radionuclides such as $^{134}Cs$, $^{135}Cs$, and $^{137}Cs$. Bioremediation processes may offer a potent method of cleaning up radioactive cesium. However, there have only been limited reports on $Cs^+$ tolerant bacteria. In this study, we report the isolation and identification of $Cs^+$ tolerant bacteria in environmental soil and sediment. The resistant $Cs^+$ isolates were screened from enrichment cultures in R2A medium supplemented with 100 mM CsCl for 72 h, followed by microbial community analysis based on sequencing analysis from 16S rRNA gene clone libraries(NCBI's BlastN). The dominant Bacillus anthracis Roh-1 and B. cereus Roh-2 were successfully isolated from the cesium enrichment culture. Importantly, B. cereus Roh-2 is resistant to 30% more $Cs^+$ than is B. anthracis Roh-1 when treated with 50 mM CsCl. Growth experiments clearly demonstrated that the isolate had a higher tolerance to $Cs^+$. In addition, we investigated the adsorption of $0.2mg\;L^{-1}$ $Cs^+$ using B. anthracis Roh-1. The maximum $Cs^+$ biosorption capacity of B. anthracis Roh-1 was $2.01mg\;g^{-1}$ at pH 10. Thus, we show that $Cs^+$ tolerant bacterial isolates could be used for bioremediation of contaminated environments.

Effects of Temperature on the Uptake and Retention of Cesium-137 by the Clam Cyclina sinensis (가무락조개에 의한 세슘-137 의 농축(濃縮)과 잔류(殘留)에 미치는 온도(溫度)의 영향)

  • Yoo, Byung-Sun;Lee, Jeong-Ho;Lee, Su-Rae
    • Korean Journal of Environmental Agriculture
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    • v.2 no.1
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    • pp.24-29
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    • 1983
  • The effects of temperature on the uptake of $^{137}Cs$ from seawater and on the retention after its uptake by the clam Cycling sinensis was investigated under laboratory conditions. The clams exhibited a greater bioaccumulation of $^{137}Cs$ in $25^{\circ}C$-acclimated animals than those acclimated at $15^{\circ}C$. The viscera of the clams reached the highest bioconcentration factor after 14 days uptake from seawater, but the tissue distribution pattern of $^{137}Cs$ was little influenced, if any, by the uptake temperature. The uptake rate slightly decreased with an increase of temperature in order of $10^{\circ}C$. The radionuclide accumulated in clams was released again in a radionuclide-free seawater according to a two-exponential compartment model. A temperature increase of $10^{\circ}C$ reduced the biological half-life of the long-lived component with a factor of about two, whereas it caused no change in the short-lived component.

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The Analytical Radioactive Waste Repository Source Term REPS Model (방사성폐기물 처분장 선원항 REPS 모델)

  • Kim, Chang-Lak;Cho, Chan-Hee;Park, Kwang-Sub;Kim, Jinwung
    • Nuclear Engineering and Technology
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    • v.22 no.4
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    • pp.315-325
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    • 1990
  • The analytical repository source term (REPS) computer code is developed for the safety assessment of radioactive waste geologic repository. For reliable prediction of the leach rates for various radionuclides, degradation of concrete structures, corrosion rate of waste container, degree of corrosion on the container surface, and the characteristics of radionuclides are considered in this REPS code. For the validation of the radionuclide leach rates predicted by the REPS model, the calculated leach rates of Cs-137, Sr-85, and Co-60 are compared with two reported leaching test results. Cesium and strontium leach congruently, and the leaching test results of these species can be reproduced by the congruent leaching model included in the REPS model. In case of cobalt, the solid diffusion model is in good agreement with the leaching test results.

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Development of gamma ray scanning coupled with computed tomographic technique to inspect a broken pipe structure inside laboratory scale vessel

  • Saengchantr, Dhanaj;Srisatit, Somyot;Chankow, Nares
    • Nuclear Engineering and Technology
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    • v.51 no.3
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    • pp.800-806
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    • 2019
  • This paper presents a laboratory experiment on data acquisition technique that applied to the gamma radiation scanning coupled with computed tomography (CT) technique for inspection of broken nozzle inside the vertical vessel. The acquisition technique was developed to inspect a large diameter vessel when suspicious problem location is not easily accessed. This technique allows the installation of gamma radiation source (Cesium 137, Cs-137), and detectors (Sodium Iodine. NaI(Tl)) from the accessible location to the required location and performs the scanning by designed pattern. To demonstrate the designed technique, top opened tank which installed with six cut steel pipes diameter of 76.2 mm (3") at a certain position was selected. They were assumed to be a gas riser pipes inside the vessel. Three studied cases were performed, (a) projection of well installed six pipes, (b) projection of one out of six broken pipe and (c) one of nozzle was assumed to be failure and fell down until one out of six pipes was broken and obstructed by nozzle. Results clearly indicated the capability of developed technique to distinguish between normal situation case and abnormal situation cases.

Monitoring of Artificial Radionuclides in Edible Mushrooms in Korea (식용 버섯류에서의 인공 방사능 농도 조사)

  • Cho, Han-Gil;Kim, Ji-eun;Lee, Sung-nam;Moon, Su-kyong;Park, Yong-Bae;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.33 no.6
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    • pp.488-494
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    • 2018
  • To ensure food-safety of mushrooms from radioactive contamination, edible mushroom samples distributed in Gyeonggi province in Korea were collected according to species and country of origin. A total of 284 mushrooms, belonging to 10 species (Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, Agaricus bisporus, Flammulina velutipes, Phellinus linteus, Inonotus Obliquus (Chaga), Auricularia auricula-judae, Ganoderma lucidum and Tricholoma matsutake) were subjected to radioactivity testing. The concentration of artificial radionuclides, 131I, 134Cs, and 137Cs, was analyzed using gamma-ray spectrometry. 131I and 134Cs were not detected more than MDA value from all samples. Among 204 domestic mushrooms, however, 137Cs were detected in 0.21~2.58 Bq/kg from six cases (3 Lentinula edodes, 1 Ganoderma lucidum and 2 Tricholoma matsutake), whereas 137Cs were detected in 0.21~53.79 Bq/kg from 38 cases (22 Inonotus Obliquus(Chaga), 14 Phellinus linteus, 1 Lentinula edodes and 1 Tricholoma matsutake) among 80 imported mushrooms. In addition, average concentration of 137Cs in 10 Chaga mushroom-processed products was more than twice as much as dried Chaga mushroom, and maximum concentration was 123.79 Bq/kg. Results suggest that radioactivity monitoring system for imported mushrooms and mushroom-processed products should be continuously intensified to secure food-safety in Korea.

Removal of Sr and Cs ions by SAN-Zeolite Beads Prepared by Immobilization of Zeolite with SAN (제올라이트를 SAN으로 고정화한 SAN-Zeolite 비드에 의한 Sr과 Cs 이온 제거)

  • Lee, Chang-Han;Kam, Sang-Kyu;Lee, Min-Gyu
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1331-1341
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    • 2015
  • The removal of Sr ion and Cs ion was investigated to evaluate adsorption properties by using SAN-Zeolite beads immobilized with styrene acrylonitrile (SAN). The adsorption capacities increased with the decrease of SAN/zeolite ratio (SAR) from 2.5 to 0.83. The relationship of adsorption capacity ($q_e$) and SAR was described by experimental equation such as $q_e=20.88+137.81e^{-1.96SAR}$ ($r^2=0.9980$). The adsorption kinetics of Sr ion and Cs ion with SAN-Zeolite beads were fitted well by the pseudo-second-order model. The maximum adsorption capacities of Sr ion and Cs ion calculated from Langmuir isotherm model were 66.97 mg/g and 81.97 mg/g, respectively.

Zoosporic Fungi Isolated From Four Egyptian Lakes and the Uptake of Radioactive Waste

  • Mahmoud, Yehia A.G.;Zeid, Alaa M. Abou
    • Mycobiology
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    • v.30 no.2
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    • pp.76-81
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    • 2002
  • Aquatic fungi from four brackish water lakes; Edku, Burullus and Manzala lakes which are located at the northern region of Egypt and Qarun lake that located in El-Fayoum city are reported in this manuscript. Twenty-nine fungal species which belong to 19 genera of aquatic fungi were recovered from water samples collected from the studied lakes. The most frequently isolated fungal species were Chytridium conferrop, Allomyces throughout and Rhizoclosmatium globosum. Thraustochytrium amoeboidum and Leptolegniella exoosporus have a moderately occurrence frequency. The maximum fungal count of recovered aquatic fungi was recorded in Burrullus lake followed by EdKu, Manzala and Qarun lakes. This study was extended to test the ability of six selected aquatic fungi(Brevilegniella keratinophila, Blastocladiella cystogena, Chytridium conferrop, Entophlyctis variabilis, Schizochytrium mangrovei and Thraustochytrium rosin), to uptake the radionuclide from their culture medium as a step to biologically treat the waste water or solution with radio-cesium and radio-cobalt. Fifty seven of Cs-137 and 35% of Co-60 could be removed from liquid waste by the selected aquatic fungi.

Environmental Radioactivity Prior to the Kori Nuclear Power Plant Operation

  • Pak, Chan-Kirl;Yang, Kyung-Rin
    • Nuclear Engineering and Technology
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    • v.10 no.1
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    • pp.13-25
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    • 1978
  • The present paper deals with the measurement of the environmental radioactivity at the Kori nuclear Power Plant site area for the Period of six years from December 1970 to December 1976. Gross alpha activity was measured in samples of airborne particulate. Gross beta measurement was performed on soil, water, airborne perticulate, pine needle, precipitation, fallout (gummed acetate paper) and various foodstuffs. Radioactivities of strontium-90 and cesium-l37 were determined by means of radiochemical analyses in samples of spinach, cabbage, barley, rice in terrestrial food, sea eel, shell fish, dulse, green laver in marine product and milk, and of fallout (cloumn), Furthermore, tritum was also analyzed in water sample of well, stream and sea by electrical enrichment.

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Radiosensitivity and Dobe-Survival Characteristics of Crypt Cells of Mouse Jejunum (마우스공장 소낭선세포의 방사선 감수성에 관한 실험적 연구)

  • Huh, Seung-Jae;Park, Charn-Il
    • Radiation Oncology Journal
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    • v.3 no.1
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    • pp.9-12
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    • 1985
  • To determine the radiosensitivity and dose-survival characteristics of jejunal crypt cells, experimental study was done using total 40 mice. Single irradiation of 1,000 rad to 1,600rad was delivered to whole bodies of mice, using a cesium 137 animal irradiator. The number of regenerating crypts per jejunal circumference was counted, by using a jejunal crypt cell assay technique, and dose response curve was measured. The average number of jejunal crypt Per circumference in control group was $140\pm10$. Mean lethal dose$(D_0)$ of moose jejunal crypt cell was 135rad.

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