• Title/Summary/Keyword: +S9 Mix

Search Result 250, Processing Time 0.029 seconds

In vitro Antimutagenic and Genotoxic Effects of Azadirachta indica Extract (님추출물의 in vitro 항돌연변이원성 및 유전독성 영향)

  • Yoon, Hyunjoo;Cho, Hyeon-Jo;Kim, Jin Hyo;Park, Kyung-Hun;Gil, Geun-Hwan;Oh, Jin-Ah;Cho, Namjun;Paik, Min-Kyoung
    • Journal of Applied Biological Chemistry
    • /
    • v.57 no.3
    • /
    • pp.219-225
    • /
    • 2014
  • Azadirachta indica extract (AIE) has been regarded as a promising source of environment-friendly organic materials owing to their low mammalian toxicity. However, quite a bit of research has been reported that AIE may cause clastogens in human lymphocytes. Therefore, this study was conducted to evaluate the antimutagenic and genotoxicity of two samples of AIE. Antimutagenic test was experimented by using bacterial reverse mutation test. In the bacterial reverse mutation test, five strains Salmonella Typhimurim of two samples of AIE in order to evaluate its mutagenic potential. Bacterial reverse mutation test was also performed on positive control and negative control groups in the presence of the metabolic activation system (S-9 mix) and metabolic non-activation system. In the chromosome aberration test, Chinese hamster lung cells were exposed to AIE for 6 or 24 h with BPS, or for 6 h with S-9 mix. Negative and positive control groups were experimented for chromosome aberration test. As a result, the number of mutated colonies induced by 4-NQO were reduced by AIE treatment in all strains, indicating that AIE may have antimutagenic effects. Bacterial reverse mutation and chromosomal aberration were not shown at all concentration of AIE, regardless of activation of the metabolic system. we concluded that two AIE samples used in this study have no genotoxic effects to human, according to the genotoxicity battery system suggested by ICH (International Conference on Harmonization).

Mutagenicity of the Material from Aspergillus to Salmonella typhimurium (Salmonella typhimurium에 대(對)한 국균생산물질(麴菌生産物質)의 변이원성(變異原性))

  • Chung, Ho-Kwon;Kim, Tae-Woon
    • Korean Journal of Food Science and Technology
    • /
    • v.14 no.1
    • /
    • pp.67-71
    • /
    • 1982
  • Mutant strans of Salmonella typhimurium which require histidine for their growth sensitively, were easily revertant and lost the histidine requirement, when the strains contacted with some new mutagen. This work was carried out to determine the mutagenicity of kojic acid and emodin for the mutant strains of Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, and TA 1538. Through the metabolic activation with liver microsome enzyme system of rat (S-9), kojic acid was recognized as a strong mutagen for the strain of TA 98, while it responsed weakly for the strain of TA 100. Without S-9 metabolic activation, kojic acid could not induce the mutation for the both strains of TA 98 and TA 100. Emodin was also recogniged as a strong mutagen for the strain of TA 1537 through the metabolic activation with S-9 mix.

  • PDF

Desmutagenicity of Enzymatically Browned Substances Obtained from the Reaction of Prunus salicina (Red) Enzyme and Polyphenols (재래종 적색자두(Prunus salicina) 효소갈변반응 생성물의 돌연변이 억제작용)

  • Ham, Seung-Shi;Hong, Eun-Hee;Omura, Hirohisa
    • Korean Journal of Food Science and Technology
    • /
    • v.19 no.3
    • /
    • pp.212-219
    • /
    • 1987
  • The rec-assay on Bacillus subtilis strains H17$({Rec}^+)$ and M45$({Rec}^-)$, the Ames test with modification of preincubation on Salmonella typhimurium TA98 and TA100 and DNA-breaking test on double strand calfthymus DNA were carried out using enzymatically browned substances obtained from the reaction of Prunus salicina (Red) enzyme and polyphenols. The spore rec-assay of enzymatic browning reaction products of pyrogallol, hydroxyhydroquinone. 3,4-dihydrohyoluene and chlorogenic acid showed non-mutagenic activity The spore rec-assay showed a little influence of ${Zn}^{2+}$ and ${Ni}^{2+}$ on the action of four kinds of enzymatic browning reaction products. The enzymatic browning reaction products of polyphenols did not show DNAbreaking activity. ${Cu}^{2+}$ of various metal ions influenced on DNA-breaking of enzymatic browning reaction products of pyrogallol. However, enzymatic browning reaction products of chlorogenic acid inhibited on DNA-breaking activity. Four kinds of enzymatic browning reaction products showed non-mutagenic activity on Salmonella typhimurium TA98 and TA100 with S-9 mix. In the mutagenicity on Salmonella typhimurium TA98 and TA100 with S-9 mix in the presence of benzo$({\alpha})$pyrene which is the carcinogenic substances, four kinds of enzymatic browning reaction products showed desmutagenic activity.

  • PDF

Mutagenecity evaluation of insecticidal 2-carbomethoxy-4-chlorodiethyl phosphate in short-term bioassays (살충성 물질 2-carbomethoxy-4-chlorodiethyl phosphate의 유전독성 평가)

  • Lee, Je-Bong;Sung, Ha-Jung;Jeong, Mi-Hye;Kwon, Oh-Kyung;Lee, Hae-Keun;Kim, Young-Koo
    • The Korean Journal of Pesticide Science
    • /
    • v.2 no.2
    • /
    • pp.53-58
    • /
    • 1998
  • For evaluating the mutagenic potential of 2-carbomethoxy-4-chlorodiethyl phosphate, three different short-term mutagenicity tests were used; Salmonella typhimurium preincubation assay with and without rat liver microsomal activation, chromosome aberration test in cultured chinese hamster lung fibroblast cell and in vivo micronucleus test in male mice bone marrow. In Salmonella typhimurium reverse mutation assay using TA98, TA100, TAl535 and TAl537, 2-carbomethoxy-4-chlorodiethyl phosphate did not show any mutagenic response in the presence and absence of S9 mix. It did not induce any significant structural chromosome aberrations in the absence of metabolic activation. In micronucleus test using ICR mice, the frequency of micronucleated polychromatic erythrocytes (MNPCE) increased in bone marrow cells treated with positive control, mitomycin-C, but 2-carbomethoxy-4-chlorodiethyl phosphate did not increase micronucleated polychromatic erythrocytes. These results indicate that 2-carbomethoxy-4-chlorodiethyl phosphate does not show any positive responses in short-term genotoxicity assays.

  • PDF

21Century of Combat Aspects of North Korean Attack Drones Through the War of the Century (21세기 전쟁을 통해 본 북한 공격 드론의 전투 양상 전망)

  • Kang-Il Seo;Sang-Keun Cho;Jong-Hoon Kim;Ki-Won Kim;Sang-Hyuk Park
    • The Journal of the Convergence on Culture Technology
    • /
    • v.9 no.3
    • /
    • pp.299-304
    • /
    • 2023
  • Recently, drones have been used as a major means of attack drones in major wars around the world, and it seems likely that they will evolve into game changers in the future. Recently, drones have been used as a major means of attack drones in major wars around the world, and it seems likely that they will evolve into game changers in the future. In the major wars of the 21century, attack drones are used for precision fire-guided or self-destruct attacks, For the purpose of cognitive warfare, its territory is expanding not only to land and air, but also to sea and water. These attack drones will perform multi-domain operations, and for this purpose, the level of autonomy will be improved and High-Low Mix We will continue to develop by strengthening concept-based scalability. North Korea has also been making considerable efforts to operate attack drones for a long time, and activities such as third-country-level self-explosive drones, artificial intelligence-based clustered self-explosive drones, and self-destructive stealth unmanned semi-submersible are expected. In addition to North Korea's provocations and attacks, it is hoped that there will be a need for active follow-up research on our military's countermeasures and utilization plans.

A National Vision of the Hydrogen Economy and Action Plan ('친환경 수소경제' 구현을 위한 마스터플랜 - 연료전지산업 및 중장기 신.재생에너지 개발비전 -)

  • Boo Kyung-Jin
    • Journal of Energy Engineering
    • /
    • v.15 no.2 s.46
    • /
    • pp.83-95
    • /
    • 2006
  • This study is to establish a national vision of the hydrogen economy and design a roadmap to materialize it. A goal is set to supply 15% of final energy consumption with hydrogen energy in Korea by 2040. Selecting the transportation sorter as the main target, more than 50% of vehicles on the road will be replaced with fuel cell vehicles (FCVs) while $20{\sim}30%$ of electricity demand in the residential and commercial sectors might be replaced with power generation by fuel cells. If this goals were attained as planned, primary energy demand would be reduced by 9%, resulting in improved energy mix in which fossil fuel consumption is greatly reduced whereas renewable energy increases by 47%. Furthermore, GHG emissions will be reduced by 20% and self-sufficiency in energy is enhanced up to 23%. If the hydrogen economy is to materialize, the government needs to implement institutional arrangements such as new legislations, organizations, and fiscal measures to facilitate the process. In addition, the private sector's participation is highly recommended to mobilize fund needed for the huge investment to build an infrastructure in preparation for the hydrogen economy. Arrangements for codes and standards are also required to promote industrialization of fuel cells and hydrogen production and consumption.

A Study on the Improvements of Strengths of Water-Permeable Concrete (투수성 콘크리트의 강도개선에 관한 연구)

  • 은재기;이철웅;김완기;조영국;소양섭
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 1999.04a
    • /
    • pp.307-312
    • /
    • 1999
  • The purpose of this study is to ascertain the strength properties of water-permeable concrete with redispersible polymer powder, silica fume and polypropylene fibers. The water-permeable concrete using rediapersibel polymer powder with a water-cement ratio of 25%, polymer-cement ratios of 0 to 10%, silica fume contents of 0 to 10% and fiber contents of 0 to 1.5% are prepared, and tested for flexural strength, compressive strength and water permeability. From the test results, improvements in the strength properties of the water-permeable concrete due to the addition of the redispersible polymer powder, silica fume and fibers are discussed. It is concluded from the test results that the superior flexural and compressive strengths of water-permeable concretes are obtained at a propylene fiber content of 1.0% with a void filling ratio of 50%. And, the water-permeable concrete having a flexural strength of 15.6~28.4kgf/$\textrm{cm}^2$, a compressive strength of 63.5~120.6kgf/$\textrm{cm}^2$, and a coefficient of permeability of 1.14~1.70cm/s at a void filling ratio of 30% can be prepared. Also water-permeable concrete having a flexural strength of 35.6~57.9kgf/$\textrm{cm}^2$, a compressive strength of 164.0~290.0kgf/$\textrm{cm}^2$, and a coefficient of permeability of 0.19~1.04cm/s at a void filling ratio of 50% can be prepared in the consideration of the mix proprotioning factors.

  • PDF

A Study on the Impervious Effect of Middle Pressure Grouting Technics in using the Environmentally Friendly Impregnation Materials (친환경 주입재를 사용한 중압 그라우팅 기법의 차수효과에 관한 연구)

  • Chun, Byung-Sik;Yeoh, Yoo-Hyen;Baek, Ki-Hyun;Choi, Choon-Sik;Jung, Jong-Ju;Do, Jong-Nam;Lim, Joo-Heon
    • Proceedings of the Korean Geotechical Society Conference
    • /
    • 2006.03a
    • /
    • pp.424-433
    • /
    • 2006
  • This paper studies the field applicability of the Special Chemical grouting Method(SCM) in reinforcing and reducing permeability of the back of an existing continuous wall. SCM uses double rod which imposes intermediate pressure$(981\sim9,810kPa)$ to disturb, cut, discharge, and mix the ground. It is observed that a bulb is formed by using cement paste and environmentally friendly injection materials with minimal alkali leaching. Uniaxial compression tests, test for chemical properties and fish poison test are performed. Test results indicate that the method results in higher durability, less leaching through use of the environmentally friendly injection material, and faster mobilization of the strength. In addition, field tests confirm the formation of the bulb and the seepage cutoff wall.

  • PDF

An experimental and numerical approach in strength prediction of reclaimed rubber concrete

  • Williams, Kanmalai C.;Partheeban, P.
    • Advances in concrete construction
    • /
    • v.6 no.1
    • /
    • pp.87-102
    • /
    • 2018
  • Utilization of waste tires may be considered as one of the solution to the problems faced by the local authorities in disposing them. Reclaimed rubber (RR) is being used in concrete for replacing conventional aggregates. This research work is focused on the strength prediction of reclaimed rubber concrete using a Genetic Algorithm (GA) for M40 grade of concrete and comparing it with experimental results. 1000 sets were taken and 100 iterations were run during training of GA models. A base study has been carried out in this research work partially replacing cement with three types of fillers such as Plaster of Paris (POP), Fly Ash (FA) and Silica Fume (SF). A total of 243 cubes were cast and tested for compression using a Universal Testing Machine. It was found that SF produced maximum strength in concrete and was used in the main study with reclaimed rubber. Tests were conducted on 81 cube samples with a combination of optimum SF percent and various proportions of RR replacing coarse aggregates in concrete mix. Compressive strength tests of concrete at 7, 14 and 28 days reveal that the maximum strength is obtained at 12 percent replacement of cement and 9 percent replacement of coarse aggregates respectively. Moreover the GA results were found to be in line with the experimental results obtained.

Butterfly Image Fashion Design in the Fashion Designer Brand 'Alexander McQueen' (패션 디자이너 브랜드 '알렉산더 맥퀸' 작품에 나타난 나비 이미지 패션 디자인)

  • jeon, Semi;Yum, Haejung
    • Journal of Fashion Business
    • /
    • v.23 no.4
    • /
    • pp.24-37
    • /
    • 2019
  • This study focused on the fashion designer brand 'Alexander McQueen' to determine how butterflies are used in modern fashion through the sensibilities of certain designers. To this end, both a literature review and empirical research were conducted. First, we examined the origin of the word and appearance characteristics of butterflies based on prior research and a book, and also surveyed the tendencies used by the fashion designer brand Alexander McQueen. Second, out of 239 items announced by the fashion designer brand "Alexander McQueen" RTW (Ready to Wear) ranging from the S/S Collection in 2008 to the 2018-9 F/W collection, 73 pieces deemed to be fashion using butterfly images were collected through www.samsung.net and www.firstview.com, then analyzed based on timing and aesthetic characteristics. Results. The analysis by time period was divided into fantasy, handicraft, mix and match, and aesthetic characteristics shown in the order of compromise beauty, rhythmical beauty, and voluptuous beauty. The purpose of this study was to determine how butterflies are expressed in fashion based on the sensibility of a specific designer in modern fashion, the fashion designer brand 'Alexander McQueen'. Based on the results of this study, we hope that the information presented herein on fashion of natural images will serve as a basic material for similar research or design ideas as an example of designs based on butterfly images.