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Effect of Abscisic Acid, Kinds and Concentrations of Osmoticum on Somatic Embryo Induction, Germination and Plantlet Regeneration in Larix kaempferi (ABA 및 삼투압제 종류 및 농도에 따른 낙엽송 (Larix kaempferi) 체세포배 유도, 발아 및 식물체 재분화 효과)

  • Kim, Yong Wook
    • Journal of Korean Society of Forest Science
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    • v.100 no.4
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    • pp.693-697
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    • 2011
  • This study was conducted to examine effects of concentrations of abscisic acid (ABA) or /kinds of osmotica on induction of somatic embryos (SEs), germination and plantlet regeneration in Japanese larch (Larix kaempferi). In comparison of duration of culture, concentrations of ABA and osmoticum, the highest induction number (191/g tissue) of the SE was showed in $60{\mu}M$ ABA+0.2 M sucrose for 4 weeks culture. However, the lowest number (3.5~23.5) of SEs was induced from $4{\mu}M$ ABA+0.1 M sucrose, regardless of culture duration for SEs induction. In comparison of germination efficiency of SEs, the highest induction frequencies of cotyledon (90.9%), hypocotyl (95.8%) and root (96.5%), respectively, were obtained from the SEs that cultured from the treatment of $60{\mu}M$ ABA+0.2 M sucrose with 5 weeks culture. In contrast, the lowest germination response was showed in SEs that induced from the treatment of $4{\mu}M$ ABA+0.1 M sucrose. In comparison of effect of different kinds/concentrations of osmotica for germination and plantlet regeneration, the best response was obtained from the treatment of 0.2 M sucrose with induction of cotyledon (98.3%), hypocotyl (78.4%), root (57.5%) and plantlet regeneration (54.8%), respectively.

Biological Control & Isolation of Chestnut Diseases by Colletotrichum Goeosporioides (밤나무 종실에 발생하는 탄저병균의 분리 및 친환경적 방제)

  • Kim, Young-Jae;Kang, Kil-Nam;Kim, Jong-Woo;Kim, Young-Myung;Lee, Sang-Hyeon;Hong, Suk-Il;Lee, Jae-Jun;Lee, Hyun-Su;Kim, Young-Kwon;Park, In-Seo;Cho, Yong-Gu
    • Korean Journal of Organic Agriculture
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    • v.21 no.4
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    • pp.725-736
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    • 2013
  • In order to acquire morphological characteristics and genetic characteristics of pathogen that causes anthrax to chestnut, anthrax was separate and identified in Gongju, chungnam chestnut plantation. Antagonistic microorganisms and plant extracts were selected for control of anthrax. Medium maturing variety treatment of 250 dilution fold in field was control at 71.2% and treatment of 500 dilution fold was control at 64.4% and treatment of 1000 dilution fold was control at 40.7%. Storage control value of Jabong in $25^{\circ}C$ after treatment in field is 61.7% at 250 dilution fold, 62.8% at 500 dilution fold, 40.9% at 1000 dilution fold treatment.

Structural Characteristics of Vascular Tissue in Carrot Seedlings with Anomalous Cotyledon Developed from Somatic Embryos (당근의 체세포배로부터 발생한 이상자엽 유식물의 유관속 조직의 구조적 특성)

  • 홍성식;소웅영
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.1
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    • pp.69-74
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    • 1998
  • The somatic embryos of Daucus carota L. cv. Hongshim developed in MS basal medium far 4 weeks had varied number of cotyledons. Palisade and spongy parenchyma of cotyledon were not clearly discriminated in all seedlings developed from the embryos. No independent existence of collateral vascular bundle was observed in all seedlings with various types of cotyledon ; instead, vascular bundles were either interconnected or partially connected with one another. Most of the cotyledonary bases on hypocotyl showed short cylinder structure which encircle plumule. The vascular tissues of cotyledonary bases and nodes of seedlings with jar-shaped or 1 cotyledon were connected in ring forms, showing the pattern of ectophloic shiphonostele, and similar ring form structure was also found in the vascular arrangement of 5 cotyledon seedlings. The vascular bundles of seedlings with 2, 3 and 4 cotyledons in many cases had independently arranged within the cotyledonary bases and nodes, showing the pattern of eustele. In hypocotyl, tetrarch or hexarch xylems prevailed in seedlings with jar-shaped cotyledon or 1 and 5 cotyledon; tetrarch xylems prevailed in 2 cotyledon seedlings; and triarch xylems prevailed in 3 cotyledon seedlings. In most of seedlings, cortex vascular bundles were dispensed in the region from cotyledonary node to hypocotyl, but double vascular bundles were also observed occasionally. In roots, diarch xylems were observed in most of seedlings with 2 cotyledons, triarch xylems in half of seedlings with 3 cotyledons, and diarch xylems in most of the remaining seedlings with the occasional occurrences of tetrarch xylems.

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On the Optimum Gas Composition for CA Storage of Shingo Pear (배(신고)의 CA저장을 위한 적정 환경가스 조성에 관하여)

  • Kim, Dong-Man;Shin, Hyun-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.4
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    • pp.396-400
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    • 1985
  • For investigation of the optimum gas composition for CA storage of Shingo pear, the pears were kept at 8 different $O_2/CO_2$ concentrations for 5 months and checked in some indices related in storability and sensory quality after storage and transfer to air at $20^{\circ}C$ for 1 week. The pears kept in all the CA storage conditions in this experiment, especially in $3%\;O_2-3%,\;CO_2$, were lower in weight loss than the pears kept in cold room. Immediately after storage decay rates of the pears stored at CA storage conditions except $3%\;O_2-3%,\;CO_2$ and $3%\;O_2-4%,\;CO_2$ were more serious than that of the pears stored at cold room, and the rates of the pears, especially stored at cold room, were rapidly increased by exposure to $20^{\circ}C$ after storage. The highest soluble solid content and firmess were shown in the pears stored at the CA storage condition of $3%\;O_2-3%,\;CO_2$, and the pears stored at the conditions of $3%\;O_2$ and $4%{\sim}5%\;CO_2$ had the highest acidity. In sensory evaluation the pears kept at the conditions of $3%O_2-3%$, 4% and 5% $CO_2$ maintained a good quality after storage and after storage and transfer to air at $20^{\circ}C$ for 1 week.

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Study on the Elimination of Heavy Metal Pollutants in the Soil by Vascular Plant in the River Area (하천유역의 관속식물을 이용한 토양내의 중금속 오염원 제거에 관한 연구)

  • 강경홍;김인성
    • Journal of Korea Soil Environment Society
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    • v.1 no.2
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    • pp.23-34
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    • 1996
  • The biological magnification of Pb, Cu, Zn and Fe in vascular plant, Persicaria thunbergii, Trapa japonica and Nymphoides peltata natually grown at the river and riverside and relationship between the concentration of heavy metals in these vascular plants and in the soil were investigated in the Chonju river, Chonju city. The biological magnification values of these metals in P thunbergii were founded as follows ; Pb, Cu, Zn and Fe were 80.4~254.6$mu\textrm{g}$/g, 284.6~688.4$mu\textrm{g}$/g, 635.5~1979.4$mu\textrm{g}$/g and 1160.0~3590.9$mu\textrm{g}$/g respectively. In the case of T. japonica, Pb, Cu, Zn and Fe were 107.8~306.0$mu\textrm{g}$/g, 282.7~963.0p$mu\textrm{g}$/g, 1328.3~3546.$mu\textrm{g}$/g and 656.8~9944.0$mu\textrm{g}$/g and in N. peltata, 140.1~ 193.9$mu\textrm{g}$/g, 187.7~327.3$mu\textrm{g}$/g, 1126.6~1723.6$mu\textrm{g}$/g and 611.7~1914.6$mu\textrm{g}$/g respectively. The amount of the biological magnification of heavy metals in the leaf, stem and root of these plants was generally increased in the sequence of leaf

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4월의 꿀벌관리

  • Lee, Seong-Bae
    • The Korea Beekeeping Bulletin
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    • s.329
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    • pp.30-33
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    • 2008
  • 4월은 유채. 개나리. 벚나무. 매화. 라일락. 오리목. 도토리. 참나무. 또는 사과. 배. 복숭아등을 비롯한 과일 나무 등 이름도 모르는 야생화를 비롯하여 밀원 식물이 1년중 제일 많이 개화된다고 해도 과언이 아닐 것이다. 기후도 적당한데다 밀원이 많다보니 1년중 번식이 제일 잘되는 시기이기도 하다.

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Proper Understanding and Globalization Strategy of Korean Ginseng (고려인삼의 약리효능의 올바른 이해와 세계화 전략)

  • Seong, Nak-Sul
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2011.10a
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    • pp.4-4
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    • 2011
  • 고려인삼의 학명은 Panax ginseng으로 그리스 어원을 해석하면 '모든 질병을 치료 할 수 있는 약재'이다. 본 발표에서는 첫째, 고려인삼의 약리효능에 대한 과학적인 연구 결과를 소개해 고려인삼에 대한 이해를 넓히고 둘째, 국제인삼시장의 현황과 중앙정부의 지원 방법을 소개하고자 한다. 고려인삼은 재배기간인 4~6년 소요되고 수삼, 백삼, 홍삼, 태극삼, 흑삼의 형태로 사용된다. 고려인삼은 사포닌, 알칼로이드, 다당체 등을 함유하고 있으며 생리활성의 유효성분은 ginsenoside로 알려져 있다. 고려인삼의 ginsenoside는 약 35종이 알려져 있고 각 ginsenoside에 따라 항아토피, 알츠하이머 예방, 항당뇨, 면역 증강효과를 보이고 있으며 아직도 그 효능에 대하여 많은 연구가 진행되고 있다. 고려인삼의 국제거래가격은 캐나다산의 4.8배, 중국산의 5.5배이고 토지 임차료는 미국, 중국의 약 5배, 캐나다의 2.6배이며 인건비는 중국의 13배에 달하나 수량성은 10a 당 450~600kg으로 미국 캐나다(800~1,000kg), 중국(950~1,250kg)의 수량성 보다 낮다. 고려인삼의 산업적 발전을 위해 중앙정부는 생산기반, 유통구조, 안전성, 과학화를 포함한 중장기적인 계획을 수립하고, 이에 따라 다양한 지원시책을 준비하고 있다.

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Effects of Krill and Cadmium on Lipid Composition of Plasma in Cholesterol-Fed Rats (크릴과 카드뮴이 콜레스텔롤 식이 흰쥐의 혈장지질에 미치는 영향)

  • 손미예;정문호;박석규;조영숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.1
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    • pp.38-43
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    • 1994
  • To investigate the effects of krill and cadmium on lipid composition of plasma in cholesterol-fed rats, male Sprague-Dawley rats were raised for 4 weeks with 8 experimental diets. The cholesterol, ${\beta}-lipoprotein$, phospholipid and triglyceride concentration of plasma in rats fed krill diets containing below 5%9w/w) krill were not significant differences in comparison with those of control diet. The ingestion of cadmium caused a significant increase in free cholesterol 91.6-fold), triglyceride 91.6-fold), ${\beta}-lipoprotein$(1.4-fold) and total cholesterol (1.3-fold) level, and then HDL-cholesterol concentration of cadmium diet was 1.6-fold lower than that of control diet. The krill supplement of cadmium diet rather resulted in a increase in total cholesterol, free cholesterol, ${\beta}-lipoprotein$ and triglyceride concentration of plasma.

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Screening of Physiological Functionality of Germinated Giant Embryonic Rices (발아 거대배아미의 생리활성 효과 검정)

  • Lee, Yun-Ri;Kang, Mi-Young;Koh, Hee-Jong;Chin, Joong-Hyoun;Nam, Seok-Hyun
    • Applied Biological Chemistry
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    • v.47 no.2
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    • pp.216-221
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    • 2004
  • Changes in physiological functionality of giant embryonic rice by germination process were investigated using 70% ethanolic extract of the rices. Physiological functionality was evaluated by determining the reducing power, phenolic compound content, GABA content, and antimutagenicity. The results showed that the order of reducing power of the non-germinated rices was Nampung giant embryonic rice>normal rice> Whachung giant embryonic rice, however, the activity was high as the order of Whachung giant embryonic rice> Nampung giant embryonic rice> normal rice by germination process. About 3-fold activity increase was observed for Whachung giant embryonic rice, while, the activity of Nampung giant embryonic rice and normal rice decreased by the same treatment. The phenolic compound content of three rice cultivars were found to be almost same levels. Germination of rice increased the content of phenolic compounds by 2.6-fo1d without any considerable changes for both Nampung giant embryonic rice and normal rice. The GABA contents was highest in Whachung giant embryonic rice, followed by Nampung giant embryonic rice, normal rice in either germination or non-germination condition. The germination increased the GABA contents by more than 2.4-fold for all rice cultivars tested. We also found an increase in the antimutagenic activity by germination process for all cultivars, where the activity was the greatest for Whachung cultivar.