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http://dx.doi.org/10.14578/jkfs.2011.100.4.20

Effect of Abscisic Acid, Kinds and Concentrations of Osmoticum on Somatic Embryo Induction, Germination and Plantlet Regeneration in Larix kaempferi  

Kim, Yong Wook (Biotechnology Division, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.100, no.4, 2011 , pp. 693-697 More about this Journal
Abstract
This study was conducted to examine effects of concentrations of abscisic acid (ABA) or /kinds of osmotica on induction of somatic embryos (SEs), germination and plantlet regeneration in Japanese larch (Larix kaempferi). In comparison of duration of culture, concentrations of ABA and osmoticum, the highest induction number (191/g tissue) of the SE was showed in $60{\mu}M$ ABA+0.2 M sucrose for 4 weeks culture. However, the lowest number (3.5~23.5) of SEs was induced from $4{\mu}M$ ABA+0.1 M sucrose, regardless of culture duration for SEs induction. In comparison of germination efficiency of SEs, the highest induction frequencies of cotyledon (90.9%), hypocotyl (95.8%) and root (96.5%), respectively, were obtained from the SEs that cultured from the treatment of $60{\mu}M$ ABA+0.2 M sucrose with 5 weeks culture. In contrast, the lowest germination response was showed in SEs that induced from the treatment of $4{\mu}M$ ABA+0.1 M sucrose. In comparison of effect of different kinds/concentrations of osmotica for germination and plantlet regeneration, the best response was obtained from the treatment of 0.2 M sucrose with induction of cotyledon (98.3%), hypocotyl (78.4%), root (57.5%) and plantlet regeneration (54.8%), respectively.
Keywords
abscisic acid; germination; Japanese larch; osmotica; somatic embryo;
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