• Title/Summary/Keyword: *-isolated points

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Antibiotics produced by anaerobic fermentation of Streptococcus sp. An-21-1 isolated from domestic soil, Fermentation and purification of antibiotics from anaerobe (국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 II. 항생물질을 생성하는 혐기성 세균의 발효 및 항생물질의 분리 정제)

  • Park, Seung-chun;Yun, Hyo-in;Oh, Tae-kwang
    • Korean Journal of Veterinary Research
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    • v.33 no.1
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    • pp.61-69
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    • 1993
  • In order to search for new antibiotics from anaerobic bacteria, a large number of samples from domestic soil were collected and processed by apropriate methods. A potential strain, Streptococcus sp. An-21-1, was found to produce antimicrobial compounds. The Results were as follows; 1. During fermentation, the bacteria grew rapidly up to 20hr, thereafter entered the death phase. The optimal temperature and pH for the bacterial growth were $37^{\circ}C$ and pH 7.0, respectively. 2. Antibiotics were purified from culture broth by solvent extraction, silica gel column chromatography and Sepadex L.H 20 column. 3. Physicochemical properties of Ap-1 and Ap-2 were similar ; Their melting points were between $234-237^{\circ}C$. Color reactions of ninhydrin, 2,7-dichlorofluorescein, 4-dimethylaminobenzaldehyde, Dragendroffs reagent and 20% $H_2SO_4$, were positive. Therefore, we assumed that these antibiotics have amine group, immine group, alkaloid, and lipid components. These were stable to heat. UV spectrophotometry showed two peaks at 210 nm and 260 nm. From above results, we assumed these antibiotics are belong to the peptide antibiotic family.

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Contact Force and Response Analysis of Vibration Isolated Systems (진동절연 시스템의 충격력과 충격응답 해석)

  • 김준호;박윤식
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.18 no.12
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    • pp.3185-3194
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    • 1994
  • In this study, an impact model and impact response analysis method was suggested for the impacts between arbitrary shaped bodies. Unlike the impacts between geometrically simple structures, there is no rules to analyze the impacts between general elastic structures First of all, it has been attempted to explain the impoot between arbitrary elastic structures as the elastic deformation of a virtual contact spring in the vicinity of contact points. The contact stiffness and the exponent are determined from the Hertz's contact theory and F. E. analysis. In order to evaluate the validities and limitations of the proposed methods, an impact tester and the miniature of container, missile and isolators have been provided and tested experimentally. All the experiments were performed with various impact conditions. The results obtained by the proposed methods were directly compared with the measured values in terms of maximum contract force, contact duration, the shape of contact force and the structure responses. The computed contact force and responses, using this proposed methods, were very close to the measured results, unless any plastic deformations were presented.

UPPER TRIANGULAR OPERATORS WITH SVEP

  • Duggal, Bhagwati Prashad
    • Journal of the Korean Mathematical Society
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    • v.47 no.2
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    • pp.235-246
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    • 2010
  • A Banach space operator A $\in$ B(X) is polaroid if the isolated points of the spectrum of A are poles of the resolvent of A; A is hereditarily polaroid, A $\in$ ($\mathcal{H}\mathcal{P}$), if every part of A is polaroid. Let $X^n\;=\;\oplus^n_{t=i}X_i$, where $X_i$ are Banach spaces, and let A denote the class of upper triangular operators A = $(A_{ij})_{1{\leq}i,j{\leq}n$, $A_{ij}\;{\in}\;B(X_j,X_i)$ and $A_{ij}$ = 0 for i > j. We prove that operators A $\in$ A such that $A_{ii}$ for all $1{\leq}i{\leq}n$, and $A^*$ have the single-valued extension property have spectral properties remarkably close to those of Jordan operators of order n and n-normal operators. Operators A $\in$ A such that $A_{ii}$ $\in$ ($\mathcal{H}\mathcal{P}$) for all $1{\leq}i{\leq}n$ are polaroid and have SVEP; hence they satisfy Weyl's theorem. Furthermore, A+R satisfies Browder's theorem for all upper triangular operators R, such that $\oplus^n_{i=1}R_{ii}$ is a Riesz operator, which commutes with A.

Computational evaluation of wind loads on a standard tall building using LES

  • Dagnew, Agerneh K.;Bitsuamlak, Girma T.
    • Wind and Structures
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    • v.18 no.5
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    • pp.567-598
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    • 2014
  • In this paper, wind induced aerodynamic loads on a standard tall building have been evaluated through large-eddy simulation (LES) technique. The flow parameters of an open terrain were recorded from the downstream of an empty boundary layer wind tunnel (BLWT) and used to prescribe the transient inlet boundary of the LES simulations. Three different numerically generated inflow boundary conditions have been investigated to assess their suitability for LES. A high frequency pressure integration (HFPI) approach has been employed to obtain the wind load. A total of 280 pressure monitoring points have been systematically distributed on the surfaces of the LES model building. Similar BLWT experiments were also done to validate the numerical results. In addition, the effects of adjacent buildings were studied. Among the three wind field generation methods (synthetic, Simirnov's, and Lund's recycling method), LES with perturbation from the synthetic random flow approach showed better agreement with the BLWT data. In general, LES predicted peak wind loads comparable with the BLWT data, with a maximum difference of 15% and an average difference of 5%, for an isolated building case and however higher estimation errors were observed for cases where adjacent buildings were placed in the vicinity of the study building.

Effects of Environmental Factors on the Cambial Electrical Resistance of Woody Plants (목본식물 형성층 전기저항에 영향을 주는 환경 요인)

  • Kim, Dong-Uk;Kim, Min-Soo;Lee, Bu-Yong
    • Journal of the Korean Institute of Landscape Architecture
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    • v.35 no.3
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    • pp.105-113
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    • 2007
  • This study was conducted to analyze the effects of environmental factors such as soil moisture, light intensity, temperature and humidity on changes in cambial electrical resistance. To improve data quality, cambial electrical resistance was continuously measured at fixed points by using a data logger isolated from alternating current. The relationship between environmental factors and changes in cambial electrical resistance was also analyzed. The results are as follows: 1. Cambial electrical resistance is highly correlated to the temperature of the measured area(r=-0.934). Therefore, temperature compensation is needed to analyze the effects of other environmental factors on cambial electrical resistance changes. 2. If temperature is compensated for, the change of cambial electrical resistance is highly correlated to water vapor pressure(r=-0.836). 3. If temperature and humidity are compensated for, the change of cambial electrical resistance is highly correlated to intensity of light(r=-0.738). 4. Diurnal deviation of soil water potential is not more significantly related than the change of cambial electrical resistance. However, in the long-term, soil water potential and cambial electrical resistance are highly correlated(r=-0.831). This indicates that soil moisture significantly influences the long-term change of cambial electrical resistance.

Characterization of Two Glucans Activating an Alternative Complement Pathway from the Fruiting Bodies of Mushroom Pleurotus ostreatus

  • Kweon, Mee-Hyang;Lim, Wang-Jin;Yang, Han-Chul;Sung, Ha-Chin
    • Journal of Microbiology and Biotechnology
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    • v.10 no.2
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    • pp.267-271
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    • 2000
  • Abstract Two glucans (PONGa and PONGb) differing in their anomeric and glycosidic linkage structures were isolated from the water-insoluble materials (PON) of Pleurotus ostreatus basidiocarps, which activated the complement system and were almost soley composed of D-glucose. The isolatIon was achieved by repeated precipitations with ethanol and adsorption on concanavalin A (Con A) of paN suspension in thymol/NaCL Based on methylation analysis. IR, GLC-MS, $^1H,{\;}and{\;}^{13}C-NMR$ spectroscopies, PONGa was found to be a branched a-glucan composed of ${\alpha}-linked$ D-glucopyranose residues and ${\alpha}-linked$ units with 6-branching points, whereas PONGb was a linear ${\beta}-1,3-glucan$ composed mainly of ${\beta}-1,3-linked$ D-glucopyranose residues. The PONGb particles reacted more potently than the PONGa particles as C3 activator in alternative complement hemolysis and crossed-immunoelectrophoresis using anti-human C3, thereby suggesting that the complement activating components of PON were ${\beta}-(13)-glucans rather$ than ${\alpha}-glucan$ components.onents.

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Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

  • Pulla, Rama Krishna;Kim, Yu-Jin;Kim, Myung-Kyum;Senthil, Kalai Selvi;In, Jun-Gyo;Yang, Deok-Chun
    • BMB Reports
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    • v.41 no.4
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    • pp.338-343
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    • 2008
  • Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.

Complete 3D Surface Reconstruction from Unstructured Point Cloud (조직화되지 않은 점군으로부터의 3차원 완전 형상 복원)

  • Li Rixie;Kim Seokil
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.4 s.235
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    • pp.570-577
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    • 2005
  • In this study a complete 3D surface reconstruction method is proposed based on the concept that the vertices of surface model can be completely matched to the unstructured point cloud. In order to generate the initial mesh model from the point cloud, the mesh subdivision of bounding box and shrink-wrapping algorithm are introduced. The control mesh model for well representing the topology of point cloud is derived from the initial mesh model by using the mesh simplification technique based on the original QEM algorithm, and the parametric surface model for approximately representing the geometry of point cloud is derived by applying the local subdivision surface fitting scheme on the control mesh model. And, to reconstruct the complete matching surface model, the insertion of isolated points on the parametric surface model and the mesh optimization are carried out Especially, the fast 3D surface reconstruction is realized by introducing the voxel-based nearest-point search algorithm, and the simulation results reveal the availability of the proposed surface reconstruction method.

Numerical and Experimental Analyses of the Aerodynamic Characteristics of a Counter Rotating Axial Fan (엇회전식 축류홴의 공력 특성에 관한 전산 해석 및 실험)

  • Cho, Jin-Soo;Won, Yu-Phil;Lee, Moon-Cheol
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.3
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    • pp.325-337
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    • 2000
  • A study was done on the numerical and experimental analyses of the aerodynamic characteristics of a counter rotating axial fan. The numerical analysis uses the frequency domain panel method developed for the aerodynamic analysis of interacting rotating systems, which is based on the unsteady lifting surface panel method. Each stage of interaction involves the solution of an isolated rotor, the interaction being done through the Fourier transform of the induced velocity field. Numerical results showed good agreements with other experimental data for single and counter rotating propeller systems. And they were compared with the experimental results of the counter rotating axial fan studied in the present paper. The performance test was carried out based on the Korean Standard (KS B 6311). It was focused on the relative efficiency increase of a counter rotating system for a single rotating one, and effects of the axial distance between the front and rear rotors on overall fan performances were investigated. As a result, it was shown that the counter rotating axial fan has the efficiency 14% higher than the single rotating one at peak efficiency points.

An Approach to Segmentation of Address Strings of unconstrained handwritten Hangul using Run-Length Code (Rum-Length code를 이용한 제약없이 쓰여진 한글 필기체 주소열 분할)

  • Kim, Gyeonghwan;Yoon, Jason-J
    • Journal of KIISE:Software and Applications
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    • v.28 no.11
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    • pp.813-821
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    • 2001
  • While recognition of isolated units of writing, such as a character or a word, has been extensively studied, emphasis on the segmentation itself has been lacking. In this paper we propose an active segmentation method for handwritten Hangul address strings based on the Run-length code. A slant correction algorithm, which is considered as an important preprocessing step for the segmentation, is presented. Three fundamental candidate estimation functions are introduced to detect the clues on touching points, and the classification of touching types is attempted depending on the structural peculiarity of Hangul. Our experiments show segmentation performance of 88.2% on touching characters with minimal over-segmentation.

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