• 한국가축번식학회지
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• 제15권3호
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• pp.221-224
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• 1991

This study was undertaken to evaluate the effect of rabbit peritioneal fluid(rPF) on in vitro maturtion of porcine follicular oocytes. From does 20h after hCG injection, rPF was aspirated aseptically at laparatomy, and then centrifuged, filtrated, and preincubated immediately for 12h. Porcine follicular oocytes isolated from ovaries of slaughtered animals were incubated in TCM-HEPES＋10% FCS, TCM-HEPES＋rPF(v/v, 50/50), or rPE only and examined the nuclear maturation after aceto-orcein or hochest staining. After identifying the optimal incubation time, this experiment was repeated for 5 times. Under the TCM-HEPES containing hormones and serum codition, the time range of porcine follicular oocyte maturation was 38 to 44 hours and the optimal time of maturation of follicular oocyte in vitro was 42 hour cultivation, respectively. The maturatin rates(89.4% and 92.7%) of porcine follicular oocytes cultured in the media with 50% rPF or only rPF were signifciantly higher thanthat (84.6%) of oocytes cultured with TCM-HEPES, respectively. These results suggest that the unknown components(s) of rPF promoted in vitro maturation of porcine follicular oocytes.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.325-334
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• 2004

본 연구는 인도산 허브를 이용하여 제조한 Animunin Powder${\circledR}$를 자돈 및 육성돈에 첨가 급여시 그 성장과 혈액내의 면역반응을 알아보고자 실시하였다. 시험 1은 3원 교잡종(Landrace ${\times}$ Yorkshire ${\times}$ Duroc) 자돈 96두를 공시하였으며 시험개시시의 체중은 평균 20.72 ${\pm}$ 2.63kg이었고 1) Con(Control),2) AB(Control + 0.1% Antibiotics), 3) AM(Control + 0.1% Animunin Powder${\circledR}$)로 4개 처리를 하여 처리당 6반복 반복당 4두씩 임의 배치하여 4주간 실시하였다. 전체 사양시험기간동안 ABM 처리구가 대조구에 비해 유의적으로 ADG와 ADFI가 높았다(P < 0.05). IgG에서는 AM 처리구와 ABM 처리구가 AB 처리구에 비해 유의적으로 높았다(P < 0.05). Albumin에서는 ABM 처리구가 CON과 AM처리구에 비해 유의적으로 높았으며(P < 0.05) lymphocyte에서는 AB 처리구와 ABM 처리구가 CON과 AM 처리구에 비해 유의적으로 높은 결과를 나타냈다(P < 0.05). 건물의 소화율에서는 ABM 처리구가 AB와 AM 처리구에 비해 유의하게 높은 결과를 보였다(P < 0.05). 시험 2는 3원 교잡종 (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) 육성돈 75두를 공시하였으며 시험개시시의 체중은 평균 40.22 ${\pm}$ 2.82kg이었고 1) CON(Control), 2) AM1(Control + 0.1% Animunin Powder${\circledR}$), 3) AM2(Control + 0.2% Animunin Powder${\circledR}$)으로 3개 처리를 하여 처리당 5반복 반복당 5두씩 임의 배치하여 29일간 실시하였다. 전체 사양시험기간 동안 AM1과 AM2 처리구가 CON 처리구에 비해 ADG가 유의하게 높았고(P < 0.05) ADFI에서는 AM1 처리구가 CON 처리구에 비해 유의하게 높은 값을 보였다(P < 0.05). Total protein에서는 AM2 처리구가 CON과 AM1 처리구 보다 유의하게 높았으며(P < 0.05) lymphocyte에서는 AM1, AM2, CON순으로 유의적인 차를 나타내었다(P < 0.05). 결론적으로 사료 내 Animunin Powder${\circledR}$의 첨가는 성장과 혈액 내 면역반응에 효과적인 것으로 사료된다.

• Biomaterials and Biomechanics in Bioengineering
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• 제2권4호
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• pp.237-248
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• 2015

"Dynamic stabilization" systems have been developed in recent years to treat degenerative disorders of the spinal column. In contrast to arthrodesis (fusion), the aim here is to conserve intervertebral mobility to maximize comfort. When developing innovative concepts, many mechanical tests need to be carried out in order to validate the different technological solutions. The present study focuses on the B Dyn$^{(R)}$ "dynamic stabilization" device (S14$^{(R)}$ Implants, Pessac, France), the aim being to optimize the choice of polymer material used for one of the implant's components. The device allows mobility but also limit the range of movement. The stiffness of the ring remains a key design factor, which has to be optimized. Phase one consisted of static tests on the implant, as a result of which a polyurethane (PU) was selected, material no.2 of the five elastomers tested. In phase two, dynamic tests were carried out. The fatigue resistance of the B Dyn$^{(R)}$ system was tested over five million cycles with the properties of the polymer elements being measured using dynamic mechanical analysis (DMA) after every million cycles. This analysis demonstrated changes in stiffness and in the damping factor which guided the choice of elastomer for the B Dyn$^{(R)}$ implant.

• Animal cells and systems
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• 제2권4호
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• pp.529-538
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• 1998

In order to analyze the intracellu1ar localization of specific RNA components of ribonucleoproteins (RNP) in Xenopus oocytes, a modified protocol of whole-mount in situ Hybridization is presented in this paper, Mitochondria specific 12S rRNA probe was used to detect the amplification and distribution of mitochondria in various stages of the oocyte life cycle, and the results were found to be consistent with previously known distribution of mitochondria. The results with other specific probes (U1 and U3 small nuclear RNAs, and 5S RNA) also indicate that this procedure is generally effective in localizing RNAs in RNP complexes even inside organelles. In addition, the RNA component of RNase MRP, the RNP with endoribo-nuclease activity, localize to the nucleus in various stages of the oocyte life cycle. Some of MRP RNA, however, were found to be localized to the special population of mitochondria near the nucleus, especially in the active stage of mitochondrial amplification. It suggests dual localization of RNase MRP in the nucleus and mitochondria, which is consistent with the proposed roles of RNase MRP in mitochondrial DNA replication and in rRNA processing in the nucleolus.

• BMB Reports
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• 제40권5호
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• pp.625-635
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• 2007

The enzyme squalene synthase (EC 2.5.1.21) catalyzes a reductive dimerization of two farnesyl diphosphate (FPP) molecules into squalene, a key precursor for the sterol and triterpene biosynthesis. A full-length cDNA encoding squalene synthase (designated as TcSqS) was isolated from Taxus cuspidata, a kind of important medicinal plants producing potent anti-cancer drug, taxol. The full-length cDNA of TcSqS was 1765 bp and contained a 1230 bp open reading frame (ORF) encoding a polypeptide of 409 amino acids. Bioinformatic analysis revealed that the deduced TcSqS protein had high similarity with other plant squalene synthases and a predicted crystal structure similar to other class I isoprenoid biosynthetic enzymes. Southern blot analysis revealed that there was one copy of TcSqS gene in the genome of T. cuspidata. Semi-quantitative RT-PCR analysis and northern blotting analysis showed that TcSqS expressed constitutively in all tested tissues, with the highest expression in roots. The promoter region of TcSqS was also isolated by genomic walking and analysis showed that several cis-acting elements were present in the promoter region. The results of treatment experiments by different signaling components including methyl-jasmonate, salicylic acid and gibberellin revealed that the TcSqS expression level of treated cells had a prominent diversity to that of control, which was consistent with the prediction results of TcSqS promoter region in the PlantCARE database.

• Food Science and Biotechnology
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• 제15권2호
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• pp.214-219
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• 2006

A bacterial strain, initially identified as B1-3, was isolated from cheonggukjang, a traditional Korean dish made from fermented soybeans. Using the Biolog system and 16S rRNA sequence analysis, we identified B1-3 as Bacillus mojavensis. We manufactured a quickly fermented soybean (QFS) food product using the B. mojavensis, and guided by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability. We isolated substances with antioxidative activity from it. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) techniques, we isolated 4 compounds from the ethyl acetate (EtOAc)-soluble neutral fraction of methyl alcohol (MeOH) extracts of the QFS food product (genistein, daidzein, 3R,4R-3-methyl-3,4-dihydroxy-2-pentanone, and 3S,4R-3-methyl-3,4-dihydroxy-2-pentanone) and 3 compounds from its acidic fraction (4-hydroxyphenylacetic acid, genistin, and daidzein). Two compounds from the neutral fraction (3R,4R-3-methyl-3,4-dihydroxy-2-pentanone and 3S,4R-3-methyl-3,4-dihydroxy-2-pentanone) were not detected in nonfermented soybeans (NFS) or in the filtrate of the LB broth used to culture B. mojavensis. However, they were detected in the filtrate of the same broth when it contained 2% glucose. These results suggest that these 2 compounds were derived from glucose (or other saccharides) in the soybean during fermentation. One compound that was found in the acidic fraction (4-hydroxyphenylacetic acid) was readily detected in NFS, but not in the culture broth. This suggests that 4-hydroxyphenylacetic acid was derived from NFS. We concluded that the antioxidative activity of cheonggukjang is a result of the interactions between soybean components and the microorganisms used in the fermentation of cheonggukjang.

• 한국ITS학회 논문지
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• 제19권3호
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• pp.137-147
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• 2020

본 논문에서는 5G 이동통신 주파수 대역에서 자동차 전장품 전자기파 내성 평가를 위한 3 ~ 5 GHz 광대역 특성의 인쇄형 bow-tie 안테나를 제안하였다. 제안된 안테나는 2.75 ~ 6 GHz 주파수 대역에서 -10 dB 대역폭을 만족하였고, 공진 주파수 대역에서 S₁₁ 특성이 -16.2 dB 이하의 특성을 보이면서 방사 패턴은 broadside한 형태를 얻었다. 또한, 실제 5G 이동통신 주파수 대역에서의 전자기파 내성 평가를 수행함에 있어서도, 국제 표준에서 규제하는 VSWR 특성이 2.1 이하의 특성을 유지하면서도 기준 레벨인 1 W의 레벨을 문제없이 형성하였다. 이러한 결과를 통하여, 제안된 안테나는 5G 이동통신 주파수 대역에서 전자기파 내성 검증을 위한 평가용 안테나로 적용할 수 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1814-1822
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• 2018

Most people use cosmetics to protect their skin. Preservatives are often used to prevent their contamination upon use. There has been a great demand for natural preservatives due to recent reports on the side effects of parabens. Therefore, we evaluated the antimicrobial activities of Lonicera japonica and Magnolia obovata extracts and determined their potential as natural preservatives. We found that the 50% ethanol extract from L. japonica had antibacterial activity only against S. aureus and P. aeruginosa, while the ethyl acetate fraction showed antimicrobial activity against all six microbial strains tested. On the other hand, the 70% ethanol extract and the ethyl acetate fraction from M. obovata showed antimicrobial activity against all six strains. A synergistic effect against S. aureus, B. subtilis, and C. albicans was confirmed when two ethyl acetate fractions having antimicrobial activity against all six strains were used in combination. Synergistic activity against B. subtilis was also confirmed through kill-time analysis. High-performance liquid chromatography was performed to identify the components of each extract. Based on the minimum inhibitory concentration and the results of a disc diffusion assay, we confirmed that caffeic acid and luteolin influenced the antimicrobial activity of L. japonica and that the antimicrobial activity of M. obovata was influenced by the interaction of magnolol and honokiol with other components. Therefore, this study suggests that the combination of L. japonica and M. obovata extracts may be used as a plant-derived natural preservative.

• 미생물학회지
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• 제39권3호
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• pp.192-196
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• 2003

다양한 유산균 자원을 확보하기 위한 일환으로, 동치미에서 유산균을 분리하였다. 동치미에서 분리된 균주는, 당이용성과 16S rRNA 유전자분석을 통하여,Lactobacillus plantarum HB1으로 밝혀졌다. 이 균주는 Gram 양성균이었으며, catalase를 갖고 있지 않았다. 16S rRNA유전자의 염기배열 120 bp을 분석해 본 결과,1～88 bp 영역에서는 기존의 것과 거의 100%의 상동성을 보여 주었고,나머지 32 bp의 영역에서는 상당한 변이를 보여 주었다. 따라서 본 균주는 기존의 L. plantarum과는 다른 균주임을 확인할 수 있었다. 동치미의 무에 포함되어 있는 질산염은 체내에서 아질산염으로 바뀐다. 아질산염은 아민과 반응을 일으켜 니트로사민이 되는데, 이 성분은 위암 발생을 일으키는 주요인자중의 하나이다. 동치미에서 분리된 본 균주의 배양액에 400 ${\mu}M$의 아질산염을 첨가한 경우 1시간30분 후에 거의 소거되었다. 아질산염 소거작용에 관여하는 본 균주 배양액 중의 특정성분의 분리, 소거작용 기작 등에 관한 보다 깊이 있는 연구가 앞으로 필요하다.

• 한국양봉학회지
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• 제34권3호
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• pp.189-195
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• 2019

본 연구는 아까시나무 꽃으로부터 발산되는 휘발성 성분을 SPME법으로 추출하여 GC/MS를 통해 분석을 시도하여, 서로 다른 수종 별로 발산하는 FVCs의 성분과 그 구성비율이 확연하게 차이가 있음을 알 수 있었다. 아까시나무, 4배체 아까시나무, 분홍아까시나무에서 FVCs 분석결과를 비교해 볼 때 차이를 보이는 주요 FVCs는 각각 Linalool (35.47%), α-Farnesene (33.94%), (E)-4,8-Dimethylnona-1,3,7-triene (37.23%)으로 나타났으며, 공통적으로 (Z)-β-ocimene이 30% 이상을 차지하고 있음을 확인하였다. 이에 따라 수종 간 FVCs 조성비의 차이가 꿀벌의 방화 행동 유도에 중요한 역할을 할 것으로 추측되며, 아까시나무의 FVCs에 대한 꿀벌의 선호도 및 방화 행동의 연관성에 대해 심층적인 연구가 수행되어야 할 것으로 보인다.