• Structural Engineering and Mechanics
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• 제37권3호
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• pp.285-296
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• 2011

The plane crack-contact problem for an infinite elastic layer with two symmetric rectangular rigid stamps on its upper and lower surfaces is considered. The elastic layer having an internal crack parallel to its surfaces is subjected to two concentrated loads p on its upper and lower surfaces trough the rigid rectangular stamps and a pair of uniform compressive stress $p_0$ along the crack surface. It is assumed that the contact between the elastic layer and the rigid stamps is frictionless and the effect of the gravity force is neglected. The problem is reduced to a system of singular integral equations in which the derivative of the crack surface displacement and the contact pressures are unknown functions. The system of singular integral equations is solved numerically by making use of an appropriate Gauss-Chebyshev integration formula. Numerical results for stress-intensity factor, critical load factor, $\mathcal{Q}_c$, causing initial closure of the crack tip, the crack surface displacements and the contact stress distribution are presented and shown graphically for various dimensionless quantities.

• Structural Engineering and Mechanics
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• 제24권2호
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• pp.213-225
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• 2006

The propagation of non-uniformly modulated, evolutionary random waves in viscoelastic, transversely isotropic, stratified materials is investigated. The theory is developed in the context of a multi-layered soil medium overlying bedrock, where the material properties of the bedrock are considered to be much stiffer than those of the soil and the power spectral density of the random excitation is assumed to be known at the bedrock. The governing differential equations are first derived in the frequency/wave-number domain so that the displacement response of the ground may be computed. The eigen-solution expansion method is then used to solve for the responses of the layers. This utilizes the precise integration method, in combination with the extended Wittrick-Williams algorithm, to obtain all the eigen-solutions of the ordinary differential equation. The recently developed pseudo-excitation method for structural random vibration is then used to determine the solution of the layered soil responses.

• 대한통합의학회지
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• 제7권4호
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• pp.23-32
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• 2019

Purpose : The purpose of this study was to evaluate the effects of multi-sensory treatment programs on attention and learning in ADHD children. Methods : The program was provided for 50 minutes twice a week for a total of 12 times over 6 weeks. The FAIR concentration test was used to identify the children's concentration of attention before and after the intervention. The children's learning ability was evaluated using K-ABC. Results : When attention was evaluated using FAIR, there was a significant increase in all dependencies of performance value (P), quality value (Q), and continuity value (C) (p>.05). In addition, when learning ability was evaluated using K-ABC, learning ability in general increased significantly (p>.05). The multi-sensory play therapy program had a positive effect on the children's attention and learning ability and thus it is a positive intervention method for children with ADHD. Conclusion : In addition to providing challenging activities, the program showed that it was possible to elicit the children's interest by engaging a variety of senses at the same time. This is believed to have motivated them internally to engage actively in the program.

• Nuclear Engineering and Technology
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• 제49권2호
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• pp.395-403
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• 2017

An efficient evaluation method for the probability of a tornado missile strike without using the Monte Carlo method is proposed in this paper. A major part of the proposed probability evaluation is based on numerical results computed using an in-house code, Tornado-borne missile analysis code, which enables us to evaluate the liftoff and flight behaviors of unconstrained objects on the ground driven by a tornado. Using the Tornado-borne missile analysis code, we can obtain a stochastic correlation between local wind speed and flight distance of each object, and this stochastic correlation is used to evaluate the conditional strike probability, $Q_V(r)$, of a missile located at position r, where the local wind speed is V. In contrast, the annual exceedance probability of local wind speed, which can be computed using a tornado hazard analysis code, is used to derive the probability density function, p(V). Then, we finally obtain the annual probability of tornado missile strike on a structure with the convolutional integration of product of $Q_V(r)$ and p(V) over V. The evaluation method is applied to a simple problem to qualitatively confirm the validity, and to quantitatively verify the results for two extreme cases in which an object is located just in the vicinity of or far away from the structure.

• 호남수학학술지
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• 제8권1호
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• pp.51-74
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• 1986

The thery of measure is significant in that we extend from it to the theory of integration. AS specific metric outer measures we can take Hausdorff outer measure and Lebesgue-Stieltjes outer measure connecting measure with monotone functions.([12]) The purpose of this paper is to find some properties of Lebesgue-Stieltjes measure by extending it from $R^1$ to $R^n(n{\geq}1)$ $({\S}3)$ and differentiation of the integral defined by Borel measure $({\S}4)$. If in detail, as follows. We proved that if $_n{\lambda}_{f}^{\ast}$ is Lebesgue-Stieltjes outer measure defined on a finite monotone increasing function $f:R{\rightarrow}R$ with the right continuity, then $$_n{\lambda}_{f}^{\ast}(I)=\prod_{j=1}^{n}(f(b_j)-f(a_j))$$, where $I={(x_1,...,x_n){\mid}a_j$<$x_j{\leq}b_j,\;j=1,...,n}$. (Theorem 3.6). We've reached the conclusion of an extension of Lebesgue Differentiation Theorem in the course of proving that the class of continuous function on $R^n$ with compact support is dense in $L^p(d{\mu})$ ($1{\leq$}p<$\infty$) (Proposition 2.4). That is, if f is locally $\mu$-integrable on $R^n$, then $\lim_{h\to\0}\left(\frac{1}{{\mu}(Q_x(h))}\right)\int_{Qx(h)}f\;d{\mu}=f(x)\;a.e.(\mu)$.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.587-601
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• 2013

During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.1172-1173
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• 2008

At the moment, the control ability of wind farms is a prime research concern for the grid integration of large wind farms, due to their required active role in the power system. As more wind turbines are installed, the power from wind energy will start to replace conventional generation units and its influence on power systems cannot be neglected. Besides, because of the intermittent nature of wind the output power of wind turbines fluctuates according to wind speed variation. Especially an isolated power system with small capacity such like Jeju needs more systematic solutions and regulations(grid code). This paper presents the idea of approach for centralized operating wind farm strategy to regulate the wind farm power production to the reference power ordered by the system operator. The doubly fed induction generator(DFIG) can control active and reactive power in feasible range. So wind farm comprised of DFIG has the possibility of a controllable component in the power system. The presented wind farm control has a hierarchical structure with both a wind farm control level and a wind turbine control level.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.66-75
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• 2016

Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.

• Structural Engineering and Mechanics
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• 제25권4호
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• pp.383-403
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• 2007

The contact problem for an elastic layer resting on an elastic half plane is considered according to the theory of elasticity with integral transformation technique. External loads P and Q are transmitted to the layer by means of two dissimilar rigid flat punches. Widths of punches are different and the thickness of the layer is h. All surfaces are frictionless and it is assumed that the layer is subjected to uniform vertical body force due to effect of gravity. The contact along the interface between elastic layer and half plane will be continuous, if the value of load factor, ${\lambda}$, is less than a critical value, ${\lambda}_{cr}$. However, if tensile tractions are not allowed on the interface, for ${\lambda}$ > ${\lambda}_{cr}$ the layer separates from the interface along a certain finite region. First the continuous contact problem is reduced to singular integral equations and solved numerically using appropriate Gauss-Chebyshev integration formulas. Initial separation loads, ${\lambda}_{cr}$, initial separation points, $x_{cr}$, are determined. Also the required distance between the punches to avoid any separation between the punches and the layer is studied and the limit distance between punches that ends interaction of punches, is investigated. Then discontinuous contact problem is formulated in terms of singular integral equations. The numerical results for initial and end points of the separation region, displacements of the region and the contact stress distribution along the interface between elastic layer and half plane is determined for various dimensionless quantities.

• Molecules and Cells
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• 제41권5호
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• pp.413-422
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• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.