• Journal of Life Science
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• v.30 no.8
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• pp.701-707
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• 2020

Gene editing technology using the clustered regularly interspaced short palindromic repeat (CRISPR) and the CRISPR associated protein (Cas)9 has been highly anticipated in developing breeding techniques. In this study, we discuss gene scissors as a tool for silkworm molecular breeding through analysis of Bombyx mori Kynurenine 3-Monooxygenase (BmKMO) gene editing using the CRISPR/Cas9 system and analysis of generational transmission through mutagenesis and selective crossing. The nucleotide sequence of the BmKMO gene was analyzed, and three guide RNAs (gRNAs) were prepared. Each synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the BM-N silkworm cell line. To edit the silkworm gene, K1P gRNA and Cas9 complexes were subsequently microinjected into the silkworm embryos; the hatching rate was 18% and the incidence of mutation was 60%. The gene mutation was verified in the heterozygous G0 generation, but no phenotypic change was observed. In homozygotes generated by self-crossing, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and could be an effective way of shortening the time required.

• Korean Journal of Applied Biomechanics
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• v.12 no.2
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• pp.179-195
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• 2002

The purpose of this research is to provide a proper model by analyzing the sports biomechanical of physical movements on the basis of the two patterns(open-stance and cross-stance) at the ready-to-start pose. The subjects for this study are composed of five male handball players from P university and five female shooting players from S university. Three-way moving actions at start(right, left, and forward) are recorded with two high-speed video cameras and measured with two Force platforms and a EMG system. Three-dimensional action analyzer, GRF system, and Whole body reaction movement system are used to figure out the moving mechanisms at the start pose. The analytic results of the moving mechanism at the start pose were as follows. 1. Through examining the three-way moving actions at start, I have found the cross-stance pose is better for the moving speed of body weight balance than the open-stance one. 175 degree of knee joint angle at "take-off" and 172 degree of hip joint angle were best for the start pose. 2. The Support time and GRF data shows that the quickest center of gravity shift was occurred when cross-stanced male subjects started to move toward his lefthand side. The quickest male's average supporting time of left and right foot is 0.19${\pm}$0.07 sec., 0.26${\pm}$0.06sec. respectively. The supporting time difference between two feet is 0.07sec. 3. Through analyzing GRF of moving actions at start pose, I have concluded that more than 1550N are overloaded on one foot at the open-stance start, and the overloaded force may cause physical injury. However, at the cross-stance pose, The GRF are properly dispersed on both feet, and maximum 1350N are loaded on one foot.

• Journal of KIISE
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• v.42 no.11
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• pp.1372-1379
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• 2015

This paper presents an analysis of feature extraction methods used for distinguishing the speech of patients with cleft palates and people with normal palates. This research is a basic study on the development of a software system for automatic recognition and restoration of speech disorders, in pursuit of improving the welfare of speech disabled persons. Monosyllable voice data for experiments were collected for three groups: normal speech, cleft palate speech, and simulated clef palate speech. The data consists of 14 basic Korean consonants, 5 complex consonants, and 7 vowels. Feature extractions are performed using three well-known methods: LPC, MFCC, and PLP. The pattern recognition process is executed using the acoustic model GMM. From our experiments, we concluded that the MFCC method is generally the most effective way to identify speech distortions. These results may contribute to the automatic detection and correction of the distorted speech of cleft palate patients, along with the development of an identification tool for levels of speech distortion.

• Strategy21
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• s.42
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• pp.318-346
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• 2017

Currently, security environmental instability is getting worse than ever in the East Asia including to Republic of Korea(ROK). Unlike several conventional issues such as maritime dispute -sometimes with islands- and competitions for getting natural resources, contemporary security dilemma issues followed by arms races among states deepens the power gap between strong and weak state within the region. It is notable that the arms races is the East Asia are mainly focused on naval power. As navy is the very possible force that influences neighboring states, submarine power is usually valued for its nature of stealth, mobile and aggression. Moreover, the submarine power is believed to be one of the highest valued weapon system since it shows actual effectiveness for influencing the other states while avoiding direct military conflicts compared to surface power. As a result, all states within the region are accelerating for getting such power these days. Japan, Most of all, is one of the leading state that aims to ensure self-survival and enlarge military influences under the US-Japan alliance by decisively supporting its power to the American containment strategy against China. In this regard, such movement surely sill influence on ROK both directly and indirectly as we sue the common field, the sea. Though, it has lots of restrictions for us to confront them with military forces as such confrontations within US-led alliances is not desirable upon considering current China and nK threats. As a result, ROK needs to limit the realm of alliance within the region while maintaining ROK-US alliance for getting national interests with both legal and justice superiority against Japan. This paper, as a result, is focused on suggesting the way to utilize submarines as a mean of naval power for both current security environments and the rising maritime threats in the East Asia. I concluded to participate ROK submarines in US-led military strategy against China by dispatching them into the East-China Sea and the North-East area of the Korean peninsula to protect both national interests and justice at the same tome. It should be one of the preemptive measure for confronting with neighboring states by utilizing strategic benefits of submarines while strengthening ROK-US alliances upon participating American Containment Strategy against China.

• Geotechnical Engineering
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• v.11 no.1
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• pp.9-22
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• 1995

The purpose of this study is to propose safety factors of pile bearing capacity based on the reliability analysis. Each prediction method involves various degrees of uncertainties. To account for these uncertainties in a systematic way, the ratios of the measured bearing capacity from pile load tests to the predicted bearing capacity are represented in the form of a probability density function. The safety factor for each design method is obtained so that the probability of pile foundation failure is less than 10-3. The Bayesian theorem is applied in a way that the distribution using static formulae is assumed to be the A-prior and the distribution using dynamic formulae or wave equation based methods is assumed to be the likelihood, and these two are combined to obtain the posterior which has the reduced uncertainty. The results of this study show that static formulae of the pile bearing capacity using the 5.p.7. N-value as well as dynamic formulae are highly unreliable and have to have the safety factor more than 7.4 : the wave equation analysis using PDA(Pile Driving Analyzer) system the most reliable with the safety factor close to 2.7. The safety factor could be reduced certain amount by adoption the Bayes methodology in pile design.

• Journal of dental hygiene science
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• v.13 no.3
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• pp.290-295
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• 2013

The purpose of this study was to investigate the shear bond strength between various commercial all-ceramic system core and veneering ceramics, and evaluate the clinical stability by comparing the conventional metal ceramic system. The test samples were divided into three groups: Ni-Cr alloy (metal bond), yttria-stabilized, tetragonal zirconia polycrystal (Y-TZP) (zirconia bond), lithium disilicate (lithium disilicate bond). The veneering porcelain recommended by the manufacturer for each type of material was fired to the core. After firing, the specimens were subjected to shear force in a universal testing machine. Load was applied at a crosshead speed of 0.50 mm/min until failure. Average shear strengths (mega pascal) were analyzed with a one-way analysis of variance and the Tukey test (${\alpha}$=0.05). The mean shear bond strength${\pm}$SD in MPa was $44.79{\pm}2.31$ in the Ni-Cr alloy group, $28.32{\pm}4.41$ in the Y-TZP group, $15.91{\pm}1.39$ in the Lithium disilicate group. The ANOVA showed a significant difference among groups (p<0.05). None of the all-ceramic system core and veneering ceramics could attain the high bond strength values of the metal ceramic combination.

• Journal of KIISE:Software and Applications
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• v.26 no.11
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• pp.1359-1371
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• 1999

대규모 네트워크 상에서 동작하는 분산 시스템의 구현을 위해 제시된 방법 중의 하나인 이동 코드 개념은 네트워크 공유 자원에 접근할 수 있는 효과적인 방법을 제시하였고 이 개념을 지원하는 많은 언어들의 개발을 가져왔다. 개발된 언어들이 가지고 있는 이동 코드를 지원하기 위한 언어 구문과 적용하려는 문제 영역의 특성을 반영한 언어 구문은 네트워크 프로그래밍을 하는데 있어서 효율과 문제 중심의 프로그램의 두 가지를 모두 가능하게 하고 있다. 본 논문에서는 현재 분산 컴퓨팅 환경에서 가장 많이 사용되고 있는 클라이언트-서버 모델을 확장하여 서버의 자원에 접근할 수 있는 또 다른 방법을 가진 모델을 제시하고, 이 모델을 표현할 수 있는 언어를 설계하였다. 설계된 언어는 이동 코드의 개념을 지원함으로써 대규모 네트워크에서 수행되는 프로그램의 작성을 가능하게 하고, 분산 범위 규칙을 채택함으로써 이동 코드의 기술을 일반 함수를 기술하듯 명확한 관점에서 할 수 있도록 하였다. 또한 네트워크 관련 자원들을 언어 구문으로 채택하여 네트워크 프로그래밍을 언어 수준에서 할 수 있도록 하였다. 언어의 이론적인 설계에 그치지 않고 설계된 언어를 수행할 수 있는 실행 시간 지원 시스템을 구현하였다. 실행 시간 지원 시스템은 언어를 해석하고 실행하는 코드 해석기와 이동 코드를 지원하는 네트워크 감독으로 구성되며 설계된 언어를 사용하여 실제로 네트워크 응용 프로그램을 작성하고 테스트 해 볼 수 있다.Abstract Some studies bring up a concept of code mobility as an innovative way to access network resources in order to develop distributed systems working on a large scale network. After that, many languages are suggested to support this concept. In these languages, language constructors for their particular application domains and mobile codes provide both problem-oriented views to the programmer and reasonable performance to the system. In this thesis, we extend the client-server model that is the most popular model in developing distributed systems these days. We propose a model to have another method to access server's resources and extend the C language to implement the proposed model for the large scale network. The new language has capability to build a software working on a large scale network by supporting mobile code and gives a consistent network programming view to the programmer by adapting distributed semantics. The language also makes network programming easy by providing network primitives at the language level. We implement a prototype of run-time system to support this language. The run-time system is composed of two major parts: code-interpreter that interprets and executes the language and network-daemon that supports mobile codes.

• Reproductive and Developmental Biology
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• v.29 no.1
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• pp.43-48
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• 2005

To search of a new porcine pheromonal odorants for biostimulation control system technologies to offer a potentially useful and practical way to improve reproductive efficiency in livestock species, the holographic quantitative structure activity relationship (HQSAR) model between odorants, 2-phenoxytetrahydrofurane (A), 2-cyclohexyl-oxytetrahydrofurane (B), derivatives and binding affinity constants (p[Od.]/sub 50/) for porcine odorant-binding protein (pOBP) as receptor of pig pheromones were derivated and disscused. The binding affinity constants of cyclohexyl substituents (A) for pOBP were higher (A>B) than that of phenyl substituents (B). It was revealed that the optimum HQSAR model XI using PLS analyses had a fragment length (5∼8) with chirality at 5 components and hologram length 97 bin, which had a cross-validated q²(predictablities) of 0.916, and a conventional correlation coefficient r² (fitness) of 0.988, respectively. From the atomic contribution, the C3 and C5 atom in 2-oxyfuryl group contributed to binding affinity constants, whereas the central carbon atom in tert-butyl group on the cyclohexyl ring and the C4 atom of furyl group parts showed no contribution.

• Proceedings of the Korea Crystallographic Association Conference
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• 2002.11a
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• pp.5-6
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• 2002

The new spectrometer for X-ray Induced Electron Emission Spectroscopy (XIEES) .has been recently developed in KRISS in collaboration with PTI (Russia). The spectrometer allows to perform research using the XAFS, SXAFS, XANES techniques (D.C.Koningsberger and R.Prins, 1988) as well as the number of techniques from XIEES field(L.A.Bakaleinikov et all, 1992). The experiments may be carried out with registration of transmitted through the sample x-rays (to investigate bulk samples) or/and total electron yield (TEY) from the sample surface that gives the high (down to several atomic mono-layers in soft x-ray region) near surface sensitivity. The combination of these methods together give the possibility to obtain a quantitative information on elemental composition, chemical state, atomic structure for powder samples and solids, including non-crystalline materials (the long range order is not required). The optical design of spectrometer is made according to Johannesson true focusing schematics and presented on the Fig.1. Five stepping motors are used to maintain the focusing condition during the photon energy scan (crystal angle, crystal position along rail, sample goniometer rail angle, sample goniometer position along rail and sample goniometer angle relatively of rail). All movements can be done independently and simultaneously that speeds up the setting of photon energy and allows the using of crystals with different Rowland radil. At present six curved crystals with different d-values and one flat synthetic multilayer are installed on revolver-type monochromator. This arrangement allows the wide range of x-rays from 100 eV up to 25 keV to be obtained. Another 4 stepping motors set exit slit width, sample angle, channeltron position and x-ray detector position. The differential pumping allows to unite vacuum chambers of spectrometer and x-ray generator avoiding the absorption of soft x-rays on Be foil of a window and in atmosphere. Another feature of vacuum system is separation of walls of vacuum chamber (which are deformed by the atmospheric pressure) from optical elements of spectrometer. This warrantees that the optical elements are precisely positioned. The detecting system of the spectrometer consists of two proportional counters, one scintillating detector and one channeltron detector. First proportional counter can be used as I/sub 0/-detector in transmission mode or by measuring the fluorescence from exit slit edge. The last installation can be used to measure the reference data (that is necessary in XANES measurements), in this case the reference sample is installed on slit knife edge. The second proportional counter measures the intensity of x-rays transmitted through the sample. The scintillating detector is used in the same way but on the air for the hard x-rays and for alignment purposes. Total electron yield from the sample is measured by channeltron. The spectrometer is fully controlled by special software that gives the high flexibility and reliability in carrying out of the experiments. Fig.2 and fig.3 present the typical XAFS spectra measured with spectrometer.

• Restorative Dentistry and Endodontics
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• v.35 no.6
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• pp.486-491
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• 2010

Objectives: This study examined the effect of 2% chlorhexidine on the ${\mu}TBS$ of a direct composite restoration using one-step self-etch adhesives on human dentin. Materials and Methods: Twenty-four extracted permanent molars were used. The teeth were assigned randomly to six groups (n = 10), according to the adhesive system and application of chlorhexidine. With or without the application of chlorhexidine, each adhesive system was applied to the dentin surface. After the bonding procedure, light-cure composite resin buildups were produced. The restored teeth were stored in distilled water at room temperature for 24 hours, and then cut and glued to the jig of the microtensile testing machine. A tensile load was applied until the specimen failed. The failure mode was examined using an operating microscope. The data was analyzed statistically using one-way ANOVA, Student's t-test (p < 0.05) and Scheffet's test. Results: Regardless of the application of chlorhexidine, the Clearfil $S^3$ Bond showed the highest ${\mu}TBS$, followed by G-Bond and Xeno V. Adhesive failure was the main failure mode of the dentin bonding agents tested with some samples showing cohesive failure. Conclusions: The application of 2% chlorhexidine did not affect the ${\mu}TBS$ of the resin composite to the dentin using a one-step self-etch adhesive.