• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.917-922
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• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

• 대한기계학회논문집A
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• 제35권8호
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• pp.913-920
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• 2011

본 논문은 4-매 주자직 유리섬유/에폭시 적층 복합재가 적용된 철도차량 경량 대차프레임의 피로 수명 및 강도 평가에 대해 기술한다. 대차프레임 경량화 재질로 적용된 유리섬유/에폭시 4-매 주자직 적층 복합재료의 피로특성 평가를 위하여 경사, 위사 그리고 $0^{\circ}/90^{\circ}$ 방향으로 적층된 시험편에 대하여 인장-압축 피로시험을 수행하였다. 유리섬유/에폭시 4-매 주자직 적층 복합재료의 피로시험은 5Hz의 주파수, -1의 응력비(R), $10^7$의 피로수명을 갖도록 하였다. 또한, JIS E 4207의 하중조건에 따른 대차프레임의 피로강도 평가를 Goodman 선도를 통하여 수행하였다. 유리섬유/에폭시 적층 복합재 경량 대차프레임의 피로수명 및 강도 평가기준을 만족하였으며, 무게를 고려할 경우 기존 대차프레임 재질인 SM490A 금속재에 비하여 우수한 피로특성을 갖는다.

• 한국세라믹학회지
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• 제41권8호
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• pp.623-627
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• 2004

Ba가 첨가된 LaBaGa $O_4$ 층상 perovskite의 생성상과 전도거동에 대해 고찰하였다. LaBaGa $O_4$의 La-site에 20at% 이하의 $Ba^{2＋}$ 이온을 첨가한 조성은 $K_2$Ni $F_4$구조 사방정 단일상을 형성하였다. 건조분위기에서 L $a_{0.8}$B $a_{1.2}$G $a_{3.9}$는 높은 산소분압영역에서 산소이온전도와 hole(p-type)전도의 혼합전도를 나타내었다. 수증기분위기에서는 수증기가 산소빈자리로 유입되어 proton전도가 발생되었으며, 온도가 낮아질수록 총전도에 대한 proton 전도도의 기여가 증가하여 35$0^{\circ}C$ 이하의 온도에서는 proton 전도가 지배적인 전도를 나타내었다. Proton 전도에 대한 활성화 에너지는 0.72 eV였다.

• 자원환경지질
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• 제18권4호
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• pp.369-379
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• 1985

The geological structures and some of the stratigraphy in the area studied are being thought to be ambiguous and controvertible. The present study intended to clarify these ambiguities by correct interpretation of the geological structures and lithostratigraphy of the area concerned. The so-called "Sambangsan formation", which was designated as an unknown age by the Taebaksan Geological Investigation Corps (1962) and as the mid Cambrian age by T. Kobayashi (1966) and I.S. Kim (1983), has been determined by the present study as the $Hongj{\breve{o}}m$ series of Carbo-Permian age resting unconformably on the Cambro-Ordovician limestone formations. This determination was supported by conodont study concurrently carried out by I.S.Kim. The so-called "Daehari formation", which was renamed by the later study group after the original "Sambangsan formation" distributed in the area from southwest of Sambangsan toward southwest to $Juch{\breve{o}}n$, possesses more or less the same lithlogy as "Sambangsan formation" of the old designation in the eastern of the area, but different lithology in the western localities where Sadong formation, the basal sandstone member of the Kobangsan formation and the green shale member of the Nokam formation are cropped out. The narrow belt of the complex mixture of the $Py{\breve{o}}ngan$ group in-between limestone formations extending over 16km with a width of 500m to 1000m was formed by the faults: the northern boundary with the limestone formations is a fault contact all the way through entire area and the southern boundary is either fault contact in most of the area and unconformity in some other area. The $Hongj{\breve{o}}m$ formation on the Mt. Sambangsan shows rather steeply dipping nearly isoclinal folds which plunges $10^{\circ}$ to $20^{\circ}$ southward. There are also field evidences that the limestone formations distributed in both north and south of the Hongjom formation (erstwhile "Sambangsan formation") along the Sambangsan ridge are the same formations and show the same folding as the $Hongj{\breve{o}}m$ formation. Therefore, these limestone formations should be rezoned in the light of the new structural interpretation although they were differently designated in the previous studies as $Py{\breve{o}}ngchang$ and $Y{\breve{o}}ngw{\breve{o}}l$-type of the Joson Group. The structures developed in the area mostly faults, which acted as one of the guides for the new interpretation of the geology and structure of the area are described and shown on the geologic map.

• 센서학회지
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• 제4권1호
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• pp.51-63
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• 1995

Chemical bath deposition 방법으로 다결정 $CdS_{1-x}Se_{x}$ 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회질 무늬를 측정하여 결정 구조를 밝혔다. $550^{\circ}C$로 열처리한 시료의 X-선 회절 무늬로부터 외삽법으로 구한 격자 상수는 CdS의 경우 $a_{0}=4.1364{\AA}$, $c_{0}=6.7129{\AA}$ 였으며 CdSe인 경우는 $a_{0}=4.3021{\AA}$, $c_{0}=7.0142{\AA}$ 였다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도 의존성을 연구하였다. 광전도 셀의 특성으로 스펙트럼 응답, 감도(${\gamma}$), 최대 허용 소비전격 및 응답시간을 측정하였다.

• Fisheries and Aquatic Sciences
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• 제26권3호
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• 천문학회지
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• 제43권1호
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• pp.9-23
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• 2010

In this paper we examined the association of Infrared Dark Cloud (IRDC) cores with YSOs and the geometric properties of the IRDC cores. For this study a total of 13,650 IRDC cores were collected mainly from the catalogs of the IRDC cores published from other studies and partially from our catalog of IRDC cores containing new 789 IRDC core candidates. The YSO candidates were searched for using the GLIMPSE, MSX, and IRAS point sources by the shape of their SED or using activity of water or methanol maser. The association of the IRDC cores with these YSOs was checked by their line-of-sight coincidence within the dimension of the IRDC core. This work found that a total of 4,110 IRDC cores have YSO candidates while 9,540 IRDC cores have no indication of the existence of YSOs. Considering the 12,200 IRDC cores within the GLIMPSE survey region for which the YSO candidates were determined with better sensitivity, we found that 4,098 IRDC cores (34%) have at least one YSO candidate and 1,072 cores among them seem to have embedded YSOs, while the rest 8,102 (66%) have no YSO candidate. Therefore, the ratio of [N(IRDC core with protostars)]/[N(IRDC core without YSO)] for 12,200 IRDC cores is about 0.13. Taking into account this ratio and typical lifetime of high-mass embedded YSOs, we suggest that the IRDC cores would spend about $10^4\sim10^5$ years to form high-mass stars. However, we should note that the GLIMPSE point sources have a minimum detectable luminosity of about $1.2 L_{\odot}$ at a typical IRDC core's distance of ~4 kpc. Therefore, the ratio given here should be a 100ver limit and the estimated lifetime of starless IRDC cores can be an upper limit. The physical parameters of the IRDC cores somewhat vary depending on how many YSO candidates the IRDC cores contain. The IRDC cores with more YSOs tend to be larger, more elongated, and have better darkness contrast than the IRDC cores with fewer or no YSOs.

• 한국정보통신학회논문지
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• 제7권4호
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• pp.691-698
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• 2003

적응 횡단선 필터에서 수렴 속도의 개선을 위해 기존의 최소 평균 자승 알고리즘을 확장한 반복적 최소 자승 알고리즘의 탭 가중치 갱신 메커니즘에 재순환 데이터 버퍼를 이용함으로서 수렴특성을 개선시키는 효율적인 기법을 제시하였다. 본 논문은 기존의 적응 횡단선 필터에 데이터 재순환 버퍼 구조를 제안하여 새로운 RLS 탭 가중치 갱신 알고리즘을 유도하여 조화 평균 학습 곡선의 평균 자승 에러 값에 대한 반복수에 대해서 데이터 재순환 버퍼를 사용한 학습 곡선의 수렴 속도가 버퍼가 없는 경우의 재순환 버퍼 RLS 알고리즘의 수렴 속도보다 비례하여 빠르게 수렴한다는 것을 수학적인 연산을 통해 증명하였다. 채널 진폭의 왜곡의 정도와 재순환 데이터 버퍼 수에 따른 평균 자승 에러에 대한 삼차원 시뮬레이션 결과로부터 고유치 확산이 증가함에 따라 특정 값에 수렴하기 위한 요구된 샘플의 반복수가 비례하여 증가하였으며, 재순환 데이터 버퍼 수 B가 증가함에 따라 요구된 샘플의 반복수가 B배만큼 감소함으로서 제안된 구조에서 RLS 가중치 갱신 알고리즘의 수렴특성이 개선됨을 입증하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1188-1193
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• 2005

An investigation was carried out to study the effect of two housing systems on physiological responses and energy expenditure of sheep in a semi-arid region of India. Two types of housing management were adopted. First was a shed- $6{\times}3\;m^2$ structure with all the four sides of 1.8 m chain link fencing with a central height of 3 m. The roof was covered with asbestos sheets and with mud floorings. Second was an open corral- $6{\times}3\;m^2$ open space with all the four sides covered with 1.8 m chain link fencing. Thirty-four (32 ewes and 2 rams) sheep of native Malpura breed aged about 18 months (body weight 28 kg ewes; 35 kg rams) were grazed together on a 35 ha plot of native range. All the sheep were grazed as a flock from 08.00 to 17.00 h during a yearlong study. The flock was divided into two groups (16 ewes+1 ram) in the evening and housed as per the systems (Shed and Open Corral). Dry and wet temperatures were recorded at 06.00 h and 21.00 h using a wet and dry bulb-thermometer both inside the shed and in the open corral and temperature humidity index (THI) was calculated. There was significant (p<0.05) difference in the THI between shed and open corral in all the seasons, indicating that shed was always warmer compared to open corral. Rectal temperature (RT) of both the groups of sheep was similar during morning as well as evening throughout the seasons. There were significant (p<0.05) differences in the skin temperature (ST) and respiration rate (RR) between the two groups at both the measurements in all the seasons. Highest energy expenditure (EE) was recorded inside the shed at 21.00 h (224 kJ/h) during monsoon and lowest at 6.00 h during winter (119 kJ/h). There was a significant (p<0.05) difference between the EE inside the shed and that in the open corral. It was concluded that housing had significant effects on the physiological responses and EE of sheep. Provision of housing at night was stressful during monsoon (with less rainfall) and summer, whereas it was protecting the sheep from acute cold during winter in a semi-arid region of India.

• 환경생물
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• 제36권2호
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• pp.140-149
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• 2018

진해만에서 출현하는 자어에 대한 계절별 군집 변동을 파악하기 위해 2015년과 2016년의 2월(겨울철), 5월(봄철), 8월(여름철), 그리고 11월(가을철)까지 계절별로 수행하였다. 조사기간 동안 출현한 자어는 종 수준까지 총 24과 28종이 출현하였다. 조사기간 동안 출현한 우점종은 청어 (Clupea pallasii), 꼼치(Liparis tanakae), 돛양태과 sp.(Callionymidae sp.), 청보리멸(Sillago japonica), 세줄베도라치(Ernogrammus hexagrammus), 그리고 멸치(Engraulis japonica)로 나타났으며, 이들 6종은 조사기간 중 출현한 자어 전체 출현개체수의 77.6%를 차지하였다. 우점종 중 청어와 꼼치는 2015년과 2016년의 겨울철에 출현한 반면에 청보리멸은 여름철에 출현하였다. 종 다양도 지수는 2월과 8월에 높았고 5월에 낮게 나타났다. 조사기간 동안 출현한 자어의 출현개체수를 바탕으로 nMDS 분석을 실시한 결과 계절에 따른 4개의 그룹으로 구분되었다. 본 연구결과 진해만의 자어 군집은 계절에 따른 수온변화와 산란에 참여하는 어종에 따라서 변동하였으며, 잠도와 칠전도 북부해역에서 높은 출현량을 보여 이 지역이 진해만의 주요 산란장으로 이용되고 있음을 의미한다.