• 한국작물학회지
• /
• 제45권2호
• /
• pp.83-87
• /
• 2000

The five phenolic compounds of peony root were isolated by Sephadex LH-20 column chromatography. Their chemical structures were identified by spectroscopic methods (UV, FT-IR, FAB-MS and $^1H$ .&^13C$-NMR). The complete structures of these compounds were elucidated to be (+)-taxifolin-3-0-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin. The concentrations of five phenolic compounds in the peony root of three Korean cultivars (Youngchonjakyak, Euisungjakyak and Jomjakyak) were determined by reverse-phase HPLC. The constituents concentration in Youngchonjakyak were generally higher than in Euisungjakyak and Jomjakyak. The concentrations of (+)-taxifolin-3-O-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin in three different cultivars were ranged 0.23-0.52%, 0.20-0.30%, 0.26-0.28%, 0.09-0.12% and 0.34-0.63%, respectively.

• Applied Biological Chemistry
• /
• 제41권4호
• /
• pp.265-269
• /
• 1998

Several flavonoid dimers were isolated and elucidated from the bark of Korean pine tree (Pinus densiflora). One of them was postulated to be a compound, epicatechin $(4{\rightarrow}7)$ 5,8,3',4'-tetrahydroxy-(2R,3R)-flavan-3-ol, whose structural determination was carried out by diverse NMR techniques.

• Preventive Nutrition and Food Science
• /
• 제10권2호
• /
• pp.118-121
• /
• 2005

Methanol extracts were prepared from loquat leaves (2 kg) and successively fractionated with hexane, chloroform, ethyl acetate, butanol, and water solvents. The ethyl acetate fraction exhibited the highest antioxidative and, antimicrobial activities. Therefore, the ethyl acetate fraction was purified and a chemical structure was identified by $^1H$ and $^{13}C-NMR$ spectra, FT-IR and EI/MS spectroscopies. The isolated antioxidative and antimicrobial substance was identified as epicatechin.

• 한국산림과학회지
• /
• 제103권1호
• /
• pp.113-121
• /
• 2014

사철나무 목부의 메탄올(MeOH) 조추출물로부터 10종의 화합물을 Column chromatography를 이용하여 분리하였으며 기기분석 결과, (-)-epicatechin, (-)-epigallocatechin, vanillic acid, 2, 4-dimethoxyphenol-1-glucoside, 2, 4, 6-trimethoxyphenol-glucoside, 5-caffeoylquinic acid, 4-caffeoylquinic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 1, 5-dicaffeoylquinic acid으로 각각 동정하였다.

• Biomolecules & Therapeutics
• /
• 제27권2호
• /
• pp.145-151
• /
• 2019

Methamphetamine (METH) acts strongly on the nervous system and damages neurons and is known to cause neurodegenerative diseases such as Alzheimer's and Parkinson's. Flavonoids, polyphenolic compounds present in green tea, red wine and several fruits exhibit antioxidant properties that protect neurons from oxidative damage and promote neuronal survival. Especially, epicatechin (EC) is a powerful flavonoid with antibacterial, antiviral, antitumor and antimutagenic effects as well as antioxidant effects. We therefore investigated whether EC could prevent METH-induced neurotoxicity using HT22 hippocampal neuronal cells. EC reduced METH-induced cell death of HT22 cells. In addition, we observed that EC abrogated the activation of ERK, p38 and inhibited the expression of CHOP and DR4. EC also reduced METH-induced ROS accumulation and MMP. These results suggest that EC may protect HT22 hippocampal neurons against METH-induced cell death by reducing ER stress and mitochondrial damage.

• Journal of the Korean Wood Science and Technology
• /
• 제34권6호
• /
• pp.61-71
• /
• 2006

국내산 주요 참나무류인 신갈나무, 상수리나무, 떡갈나무, 졸참나무, 갈참나무 및 상수리나무 잎의 추출성분의 구조를 규명하고 수종 상호간의 성분의 특성 및 상호 연관성 등을 조사하였다. 신갈나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, kaempferol, a stragalin, astragalin-6"-O-gallate, isoquercirin, isoquercitrin-6"-O-gallate 및 myricetin 등 10종의 화합물이 단리되었고 상수리나무에서는 gallic acid, kaempferol과 quercetin이 떡갈나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, (+)-galocatechin, (-)-epigallocatechin, kaempferol, quercetin, guajaverin 및 tamarixin 등 9종의 화합물이 단리되었으며 갈참나무에서는 gallic acid, caffeic acid, astragalin, quercetin과 isoquercitrin이 졸참나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, kaempferol, quercitrin, isoquercitrin 및 myricetin 등 7종의 화합물이 단리되었고 굴참나무에서는 gallic acid, (+)-catechin, astragalin, astagalin-6"-O-gallate와 isoquercitrin 등 5종의 화합물이 단리되었다. Gallic aicd는 모든 수종에서 단리되어 참나무류 잎 추출성분의 지표 화합물로 이용될 수 있다.

• 한국식품과학회지
• /
• 제37권4호
• /
• pp.513-518
• /
• 2005

포도종실추출물에 함유되어 강력한 항산화 작용을 나타내는 성분인 proanthocyanin의 구성 성분이면서 에피머인 (+)-catechin과 (-)-epicatechin을 CE법을 이용하여 분석하였다. CE법을 이용한 catechin류 분석의 최적 조건은 fused silica capillary$(20cm{\times}50{\mu}m)$, current $56{\mu}A$, voltage 10 kV, phosphate/borate buffer(pH 6.0), 온도 $20^{\circ}C$로서 (+)-catechin과 (-)-epicatechin을 6분 이내에 매트릭스의 방해없이 분석할 수 있었다. 비색법, HPLC법과 CE분석법의 특징을 비교해 보면 비색법은 (+)-catechin과 (-)-epicatechin합한 총 함량은 알 수 있으나 두성분의 분리는 할 수 없었다. 또한 HPLC방법은 표준품은 두 성분이 잘 분리되었으나 시료 분석 시 proanthocyanin을 가수분해하기 위해 첨가한 산으로 인해 시료가 강산성을 띠어 컬럼 손상되는 문제가 있어 직접 분석이 불가능하여 시료를 희석해야 했으며, 희석을 하여 분석할 경우에도 많은 잡 피크들로 정량적인 결과를 얻기에 용이하지 않았다. 한편 CE법의 경우 산 가수분해한 시료를 별다른 처리 없이도 다른 성분의 방해 없이 분석할 수 있었다. 분석소요시간의 경우 비색법은 실험단계가 복잡하여 많은 시간이 걸렸으며 HPLC는 15분 그리고 CE는 6분 이내에 분석이 가능하였다. 재현성과 직선성을 보면 HPLC와 CE의 방법이 모두 양호하였고 검출한계에 있어서는 CE법이 0.035mg/L로서 검출감도가 매우 우수하였다. 이와 같이 CE법은 포도종실에 존재하는 (+)-catechin과 (-)-epicatechln을 분석하는데 방법의 용이성, 분석결과의 정확성에 있어서 기존 방법인 비색법과 HPLC 방법보다 우수한 결과를 나타내었다.

• 한국잠사곤충학회지
• /
• 제42권1호
• /
• pp.6-9
• /
• 2000

This study was performed to investigate the catechins which separated from mulberry leaf. The Epicatechin contents in mulberry leves was 1.21${\pm}$0.05 $\mu\textrm{g}$/mg ethylacetate fraction. epigalocatechingallte contents WAS 3.56 $\mu\textrm{g}$/mg ethylacetate fraction. The cathechins (epicatechin and epigalocate-chingallate) separated from mulberry leaf was shown a strong activity to growth inhibition by the concentration of 5 mg/disc on the Clostridium perfringens.

• 약학회지
• /
• 제37권5호
• /
• pp.476-482
• /
• 1993

For the investigation of medicinal resources in Crataegus species, the present study was carried out to evaluate the pharmaco-constituents in the leaves of Crataegus Pinnatifida var. psilosa (Rosaceae). Fruits has folk medicine in Korea. From the $Et_{2}$O fraction of MeOH extract, a new compound, apigenin-8-C-(6"-acetyl)-$\beta$-D-glucopyranoside, named psilosin and three known compounds, ursolic acid, phytosterol complex and (-)-epicatechin were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences (UN, IR, NMR and mass etc.) in comparison with authentics, respectively.

• 생약학회지
• /
• 제27권3호
• /
• pp.219-223
• /
• 1996

From the rachis of Cedrela sinensis A. Juss., methyl gallate, quercitrin, bis-(p-hydroxyphenyl)ether, adenosine, isoquercitrin, rutin, (+)-catechin and (-)-epicatechin were isolated and characterized by spectral data.