• Biomolecules & Therapeutics
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• v.24 no.6
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• pp.595-603
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• 2016

(E)-3-Phenyl-1-(2-pyrrolyl)-2-propenone (PPP) is a pyrrole derivative of chalcone, in which the B-ring of chalcone linked to ${\beta}$-carbon is replaced by pyrrole group. While pyrrole has been studied for possible Src inhibition activity, chalcone, especially the substituents on the B-ring, has shown pharmaceutical, anti-inflammatory, and anti-oxidant properties via inhibition of NF-${\kappa}B$ activity. Our study is aimed to investigate whether this novel synthetic compound retains or enhances the pharmaceutically beneficial activities from the both structures. For this purpose, inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 cells were analyzed. Nitric oxide (NO) production, inducible NO synthase (iNOS) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) mRNA expression, and the intracellular inflammatory signaling cascade were measured. Interestingly, PPP strongly inhibited NO release in a dose-dependent manner. To further investigate this anti-inflammatory activity, we identified molecular pathways by immunoblot analyses of nuclear fractions and whole cell lysates prepared from LPS-stimulated RAW264.7 cells with or without PPP pretreatment. The nuclear levels of p50, c-Jun, and c-Fos were significantly inhibited when cells were exposed to PPP. Moreover, according to the luciferase reporter gene assay after cotransfection with either TRIF or MyD88 in HEK293 cells, NF-${\kappa}B$-mediated luciferase activity dose-dependently diminished. Additionally, it was confirmed that PPP dampens the upstream signaling cascade of NF-${\kappa}B$ and AP-1 activation. Thus, PPP inhibited Syk, Src, and TAK1 activities induced by LPS or induced by overexpression of these genes. Therefore, our results suggest that PPP displays anti-inflammatory activity via inhibition of Syk, Src, and TAK1 activity, which may be developed as a novel anti-inflammatory drug.

• Journal of Astronomy and Space Sciences
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• v.29 no.3
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• pp.269-274
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• 2012

Kinematic global positioning system precise point positioning (GPS PPP) technology is widely used to the several area such as monitoring of crustal movement and precise orbit determination (POD) using the dual-frequency GPS observations. In this study we developed a kinematic PPP technology and applied 3-pass (forward/backward/forward) filter for the stabilization of the initial state of the parameters to be estimated. For verification of results, we obtained GPS data sets from six international GPS reference stations (ALGO, AMC2, BJFS, GRAZ, IENG and TSKB) and processed in daily basis by using the developed software. As a result, the mean position errors by kinematic PPP showed 0.51 cm in the east-west direction, 0.31 cm in the north-south direction and 1.02 cm in the up-down direction. The root mean square values produced from them were 1.59 cm for the east-west component, 1.26 cm for the south-west component and 2.95 cm for the up-down component.

• Journal of IKEEE
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• v.5 no.1 s.8
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• pp.8-15
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• 2001

This paper remarks IETF based requirements for IP/UDP/RTP header compression issued in 3GPP2 All IP Ad Hoc Meeting and protocol stacks of the next generation mobile station. All IP Network, for real time application such as Voice over IP (VoIP) multimedia services based on 3GPP2 3G cdma2000. Frames for various protocols expected in the All IP network Mobile Station (MS) are explained with several figures including the bit-for-bit notation of header format based on IETF draft of Robust Header Compression Working Group (ROHC). Especially, this paper includes problems of IS-707 Radio Link Protocol (RLP) for header compression which will be expected to modify in All IP network MS's medium access layer to accommodate real time packet data service[1]. And also, since PPP has also many problems in header compression and mobility aspects in MS protocol stacks for 3G cdma2000 packet data network based on Mobile IP (PN-4286)[2], we introduce the problem of solution for header compression of PPP. Finally. we suggest the guidelines for All IP network MS header compression about expected protocol stacks, radio resource efficiency and performance.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.2
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• pp.155-161
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• 2014

Overexpression of amyloid precursor protein with the Swedish mutation causes abnormal hyperphosphorylation of the microtubule-associated protein tau. Hyperphosphorylated isoforms of tau are major components of neurofibrillary tangles, which are histopathological hallmarks of Alzheimer's disease. Protein phosphatase 2A (PP2A), a major tau protein phosphatase, consists of a structural A subunit, catalytic C subunit, and a variety of regulatory B subunits. The B subunits have been reported to modulate function of the PP2A holoenzyme by regulating substrate binding, enzyme activity, and subcellular localization. In the current study, we characterized regulatory B subunit-specific regulation of tau protein phosphorylation. We showed that the PP2A B subunit PPP2R2A mediated dephosphorylation of tau protein at Ser-199, Ser-202/Thr-205, Thr-231, Ser-262, and Ser-422. Down-regulation of PPP2R5D expression decreased tau phosphorylation at Ser-202/Thr-205, Thr-231, and Ser-422, which indicates activation of the tau kinase glycogen synthase kinase 3 beta ($GSK3{\beta}$) by PP2A with PPP2R5D subunit. The level of activating phosphorylation of the $GSK3{\beta}$ kinase Akt at Thr-308 and Ser-473 were both increased by PPP2R5D knockdown. We also characterized B subunit-specific phosphorylation sites in tau using mass spectrometric analysis. Liquid chromatography-mass spectrometry revealed that the phosphorylation status of the tau protein may be affected by PP2A, depending on the specific B subunits. These studies further our understanding of the function of various B subunits in mediating site-specific regulation of tau protein phosphorylation.

• Journal of Astronomy and Space Sciences
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• v.28 no.3
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• pp.217-223
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• 2011

Precise point positioning (PPP) is increasingly used in several parts such as monitoring of crustal movement and maintaining an international terrestrial reference frame using global positioning system (GPS) measurements. An accuracy of PPP data processing has been increased due to the use of the more precise satellite orbit/clock products. In this study we developed PPP algorithm that utilizes data collected by a GPS receiver. The measurement error modelling including the tropospheric error and the tidal model in data processing was considered to improve the positioning accuracy. The extended Kalman filter has been also employed to estimate the state parameters such as positioning information and float ambiguities. For the verification, we compared our results to other of International GNSS Service analysis center. As a result, the mean errors of the estimated position on the East-West, North-South and Up-Down direction for the five days were 0.9 cm, 0.32 cm, and 1.14 cm in 95% confidence level.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.6
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• pp.521-531
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• 2020

QZSS (Quasi-Zenith Satellite System) provides the CLAS (Centimeter Level Augmentation Service) through the satellite's L6 band. CLAS provides correction messages called C-SSR (Compact - State Space Representation) for GPS (Global Positioning System), Galileo and QZSS. In this study, CLAS messages were received by using the AsteRx4 of Septentrio which is a GPS receiver capable of receiving L6 bands, and the messages were decoded to acquire C-SSR. In addition, Multi-GNSS (Global Navigation Satellite System) Code-PPP (Precise Point Positioning) was developed to compensate for GNSS errors by using C-SSR to pseudo-range measurements of GPS, Galileo and QZSS. And non-linear least squares estimation was used to estimate the three-dimensional position of the receiver and the receiver time errors of the GNSS constellations. To evaluate the accuracy of the algorithms developed, static positioning was performed on TSK2 (Tsukuba), one of the IGS (International GNSS Service) sites, and kinematic positioning was performed while driving around the Ina River in Kawanishi. As a result, for the static positioning, the mean RMSE (Root Mean Square Error) for all data sets was 0.35 m in the horizontal direction ad 0.57 m in the vertical direction. And for the kinematic positioning, the accuracy was approximately 0.82 m in horizontal direction and 3.56 m in vertical direction compared o the RTK-FIX values of VRS.

• KDI Journal of Economic Policy
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• v.35 no.3
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• pp.1-38
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• 2013

This study investigates the welfare implications of BTL projects using a general equilibrium model with the public sector and public-private partnerships. We show that when the government is not allowed to run budget deficits but private firms is able to overcome the financial constraint, BTL projects performed by public-private partnerships (PPPs) could be a good alternative and improve the welfare of the model economy. This paper quantitatively investigates excessive expansion of PPP projects and several alternatives to retrieve welfare losses caused by such an expansion. Assuming that future rents of BTL projects are not taken into account, we find the welfare losses up to 20 percent relative to conventional government projects. Finally, we show that it would be possible to reduce the losses by transforming the fully depreciated capital stock of the government projects into much smaller new PPP projects.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11b
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• pp.1067-1070
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• 2002

EAP(Extensible Authentication Protocol)[3]은 다양한 인증 방법을 제공하기 위한 표준 인증 메커니즘이다. EAP는 PPP[16], 802.1x[17] 등에서 사용되며, 실제 인증 능력을 가지는 TLS[2] 등과 같은 인증 프로토콜과 결합하여 사용된다. TLS(Transport Layer Security)는 두 peer 간에 전송계층에서 상호 인증, 무결성, 기밀성을 제공하기 위해 개발되었다. TLS 는 상호 인증을 위하여 공개키 기반 인증서를 사용한다. 그러나, 인증서를 사용하는 것은 대부분의 사용자들이 ID, password 기반의 응용에 익숙하다는 것을 생각하면 일반적인 인증 방법이 아님을 알 수 있다. 따라서, EAP-TLS와 같은 인증 방법 역시 그런 면에서 PPP 혹은 802.1x에서 사용하기에 부적합하다고 볼 수 있다. 본 논문에서는 패스워드 기반 인증 및 키교환 프레임웍을 TLS 에 적용하고 이를 다시 EAP 와 결합한다. 패스워드 기반 EAP-TLS 는 인증서에 관련된 복잡한 연산을 수행하지 않으면서도 안전성에 있어 수학적 증명을 가지며, PPP 혹은 802.1x 서비스를 받고자 하는 사용자가 인증서를 가질 필요가 없는 장점을 가지게 된다.

• Transportation Technology and Policy
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• v.11 no.3
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• pp.36-45
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• 2014

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.3D
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• pp.381-389
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• 2009

Korean government is investing 1.8 billion won on infrastructure and investment on PPP projects constitutes 16.3%. This phenomenon is to promote private investment as well as lessening government burdens of public budgeting. However, the only criterion to be considered is government financial status in selecting public or private highway investment projects. So systematic decision support model is required in choosing public or private highway investment projects. So, this paper suggests a systematic decision support model for deciding public or private highway investment at the early stage of project planning. Furthermore, this paper identifies key decision variables with respect to economic, politic, project management criterions based on the related literatures and feedbacks from experts. This paper analyzed 30 cases of government investment and PPP projects and got the survey result from highway specialists. As a result, this paper presents an interval with respect to economic criteria using mean and standard deviation and a logistic regression equation which can predict the possibility of PPP project. Through this study, decision maker of central or local government can decide public or PPP highway project more systematically and reasonably.