• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1291-1299
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• 2007

Two ${\beta}$-1,4-glucanases (DI and DIII fractions) were purified to homogeneity from the culture filtrate of a cellulolytic bacteria, Cellulomonas sp. CS 1-1, which was classified as a novel species belonging to Cellulomonas uda based on chemotaxanomic and phylogenetic analyses. The molecular mass was estimated as 50,000 Da and 52,000 Da for DI and DIII, respectively. Moreover, DIII was identified as a glycoprotein with a pI of 3.8, and DI was identified as a non-glycoprotein with a pI of 5.3. When comparing the ratio of the CMC-saccharifying activity and CMC-liquefying activity, DI exhibited a steep slope, characteristic of an endoglucanase, whereas DIII exhibited a low slope, characteristic of an exoglucanase. The substrate specificity of the purified enzymes revealed that DI efficiently hydrolyzed CMC as well as xylan, whereas DIII exhibited a high activity on microcrystalline celluloses, such as Sigmacells. A comparison of the hydrolysis patterns for pNP-glucosides (DP 2-5) using an HPLC analysis demonstrated that the halosidic bond 3 from the nonreducing end was the preferential cleavage site for DI, whereas bond 2, from which the cellobiose unit is split off, was the preferential cleavage site for DIII. The partial N-terminal amino acid sequences for the purified enzymes were $^1Ala-Gly-Ser-Thr-Leu-Gln-Ala-Ala-Ala-Ser-Glu-Ser-Gly-Arg-Tyr^{15}$-for DI and $^1Ala-Asp-Ser-Asp-Phe-Asn-Leu-Tyr-Val-Ala-Glu-Asn-Ala-Met-Lys^{15}$-for DIII. The apparent sequences exhibited high sequence similarities with other bacterial ${\beta}$-1,4-glucanases as well as ${\beta}$-1,4-xylanases.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 1991.11a
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• pp.134-143
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• 1991

코드분할 다중통신(Code Division Multiple Access Communication)은 Spread Spectrum 통신의 발전된 형태로서 군사목적상 이동 무선통신(Combat Multiple Communication)을 위해서 최근들어 급속도로 중요시 되고있다. 잘 알려진 바와 같이 두 가지 가장 일반적인 다중통신 방식은 주파수 분할 다중통신방식과 시분할 다중 통신 방식이다. 주파수 분할 다중통신 방식에서는 모든 사용자가 각각 다른 주파수를 사용하여 동시에 신호를 보내고 시분할 다중통신에서는 같은 주파수를 사용하되 시간대를 달리하고 있다. 이 두가지 방식을 결합하여 모든 사용자가 같은 시각과 같은 주파수를 공유하여 통신을 가능케 할 수 있는 방식이 코드분할 다중통신이다. 여기에서 사용자는 각각 고유한 코드를 부여받는다. 이때 각 코드간에는 낮은 상관계수(Cross-correlation )를 가져야 하며, 이러한 성질을 만족하는 코드 집합을 구하기 위한 노력이 경주되었으며 현재까지 2진법 코드 집합으로서 Gold, Kasami, No 코드가 알려져 있으며, 복잡성(Complexity)을 증가시키기 위한 노력으로서 (군사목적에 중요) 다진법 코드에 대한 연구가 활발해지고 있으나 공개된 것은 없었다. 최근에 (1990), 다진법 코드로서 Kumar 코드가 발표되었다. 그러나 이러한 코드들은 복잡성(Complexity or Linear Span)을 증가시키지는 못하였다. 본 논문은 복잡성(Complexity)이 증가한 새로운 다진법 코드의 집합을 제안하였다. 각 집합은 $P^{n}$ 개의 코드로 구성되며, 최대 상관계수 값은 $f^{m}$ (p-1)-1 이다. (n=2m)

• Journal of Life Science
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• v.7 no.3
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• pp.167-171
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• 1997

As an initial effort to elucidate RNA: protein binding in a way to regulate translation initiation and phosphorylation, a cDNA encoding a double-stranded RNA binding factor (RBFII)was isolated from Hela ZAPII cDNA library by affinity screening using [$\alpha$$^{-32}$P] UMP-labeled HIV Rev-responsive element(RRE) RNA. The nucleotide sequence of RBF (or TRBP) cDNA except the 5’end. At the 5’end, This common ORF was fused in-frame to N-terminal residues of Lac-Z through a unique 138 nt sequence encoding 46residues in the case of RBFII and a 63 nt sequence encoding 21 residuces in the case of RBFI. The context of ATG appearing first in the sequences suggests that both these cDNA inserts are incomplete at the 5’end.

• Research in Plant Disease
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• v.24 no.2
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• pp.138-144
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• 2018

In December 2016, stem rot symptoms were observed on Persian buttercup (Ranunculus asiaticus) plants in Chilgok, Gyeongbuk, Korea. In the early stage of the disease, several black spots appeared on the stem of infected plants. As the disease progressed, the infected stem cleaved and wilted. The causal agent was isolated from a lesion and incubated on Reasoner's 2A (R2A) agar at $25^{\circ}C$. Total genomic DNA was extracted for phylogenetic analysis. Based on the 16S rRNA gene analysis, the isolated strain was found to belong to the genus Pseudomonas. To identify the isolated bacterial strain at the species level, the nucleotide sequences of the gyrase B (gyrB) and RNA polymerase D (rpoD) genes were obtained and compared with the sequences in the GenBank database. As the result, the causal agent of the stem rot disease was identified as Pseudomonas marginalis. To determine the pathogenicity of the isolated bacterial strain, it was inoculated into the stem of healthy R. asiaticus plant, the inoculated plant showed a lesion with the same characteristics as the naturally infected plant. Based on these results, this is the first report of bacterial stem rot on R. asiaticus caused by P. marginalis in Korea.

• Journal of the Korea Society of Computer and Information
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• v.4 no.3
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• pp.28-34
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• 1999

In this paper. we propose the method of Scene Change Detection for video sequence using the DC components and the moving vectors in the Macro Blocks in the DCT blocks. The proposed method detects the Scene Change which would not be related with the specific sequences in the compressed MPEG domain. To do this. we define new metrics for Scene Change Detection using the features of picture component and detect the exact Scene Change point of B-pictures using the characteristics of B-picture's sharp response for the moving vectors. In brief, we will detect the cut point using I-picture and the gradual scene changes such as dissolve, fade, wipe, etc. As a results, our proposed method shows good test results for the various MPEG sequences.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.1
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• pp.78-85
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• 2000

Due to environmental proessures there is increasing use of hydrogen peroxide as a total or partial substitute for chlorine based bleaching agents within ECF or TCF sequences. However, to aceive satifactory brightness using peroxide alone, stages having a combination of high temperature, pressure, pH or residence time are required. It may also have negative impact on fiber quality . Therefore, it would be of advantage if vertain means could be found to make hydrogen peroxide more effective in bleacing , via shortening treaction time and allevaiating the need for such forcing reaction conditions. This can be achieve by converting the peroxide in-situ to stronger oxidant through the use of 손 bleach activator. In this study to investigate the influence of additives, such as tetraacetylethylenediamine (TAED) and Molybdate (MO) . addition on peroxide bleaching were carried out. Under alkaline conditions the bleching additives. TAED and Mo. can react H2O2 to form peracetic acid and peroxomolybdate respectively and these generated activators can improve deliginification,. The activators make it possible to bleach the pulp efficiently at low temperature in the range 50 to 7$0^{\circ}C$. Also, addition of TAED and Mo is an environmentally friendly way of enhancing the performance of peroxide bleaching can be incorporated into TCF and ECF sequences.

• Animal cells and systems
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• v.4 no.3
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• pp.267-272
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• 2000

Partial sequence of the mitochondrial cytochrome b gene was analyzed to investigate genetic variation from 10 geographic populations of Granulilittorina exigua in Korea. The sequence of 282 base pairs was determined by PCR-directed silver sequencing method. The sequences of two species within the genus Littorina reserved in NIH blast search were utilized to determine geographic variations of species referred. The levels of mtDNA sequence differences were 0.00-2.54% within populations and 0.71-4.43% between populations. There were four amino acid differences between representative species of the genera Granulilittorina and Littorina, but no differences within populations of the genus Granulilittorina. The UPGMA and the N-J trees based on Tamura-Nei genetic distance matrix were constructed, which showed that the genus Granulilittorina was divided into three groups such as eastern (even exception for Tokdo population), southern, and western regional populations. The degrees of genetic divergence within populations of each group were p=0.021, p=0.019, and p=0.018, respectively. The divergence between the eastern and southern populations was p=0.032, showing closer relationship than with the western populations (p=0.052). Based on the diverged time estimation, the eastern and southern populations of Granulilittorina exigua in Korea diverged from the western populations about 2.1 MYBP, and the eastern and southern populations diverged from each other about 1.3 MYBP.

• Research in Plant Disease
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• v.29 no.1
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• pp.72-78
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• 2023

In September 2021, gray-to-brown discoloration and expanding water-soaked lesions were observed on the outer and inner layers and the core of kimchi cabbage (Brassica rapa subsp. pekinensis) in fields located in Samcheok, Gangwondo, Korea. A bacterial strain designated as KNUB-02-21 was isolated from infected cabbage samples. Phylogenetic analysis based on the sequences of the 16S rRNA region and the dnaX, leuS, and recA genes confirmed that the strain was affiliated with Pectobacterium versatile. Additionally, the biochemical and morphological profiles of the isolate were similar to those of P. versatile. Based on these results, the isolate was identified as a novel strain of P. versatile. Healthy kimchi cabbage slices developed soft rot upon inoculation with P. versatile KNUB-02-21 and exhibited symptoms similar to those observed in the diseased plants in fields. The re-isolated strains were similar to those of P. versatile. Prior to our study, P. versatile as the causative pathogen of kimchi cabbage soft rot had not been reported in Korea.

• Journal of Life Science
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• v.26 no.10
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• pp.1113-1120
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• 2016

Two bacterial strains producing extracellular man nanase were isolated from doenjang, a traditionally fermented soybean paste in Korea. The isolates, WL-6 and WL-11, were identified as Bacillus subtiis on the basis of their 16S rRNA gene sequences, morphological, and biochemical properties. Two genes encoding the mannanase of both B. subtilis WL-6 and B. subtilis WL-11 were each cloned into Escherichia coli, and their nucleotide sequences were determined. Both mannanase genes consisted of 1,086 nucleotides, encoding polypeptides of 362 amino acid residues. The deduced amino acid sequences of the two WL-6 and WL-11 mannanases, designated Man6 and Man11, respectively, differed from each other by eight amino acid residues, and they were highly homologous to those of mannanases belonging to the glycosyl hydrolase family 26. The 26 amino acid stretch in the N-terminus of Man6 and Man11 was a predicted signal peptide. Both Man6 and Man11 were localized at the level of 94–95% in an intracellular fraction of recombinant E. coli cells. The enzymes hydrolyzed both locust bean gum and mannooligosaccharides, including mannotriose, mannotetraose, mannopentaose, and mannohexaose, forming mannobiose and mannotriose as predominant products. The optimal reaction conditions were 55°C and pH 6.0 for Man6, and 60°C and pH 5.5 for Man11. Man11 was more stable than Man6 at high temperatures.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.3
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• pp.401-417
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• 1994

In this paper, a new testable design technique for domino CMOS circuits is proposed to detect stuck-at(s-at), stuck-open(s-op) and stuck-on(s-on) faults in the circuits by observing logic test reponses. The proposed technique adds one pMOS transistor per domino CMOS gate for s-op and s-on faults testing of nMOS transistors and one nMOS transistors and one nMOS transistor per domino gate or multilevel circuit to detect s-on faults in pMOS transistors of inverters in the circuit. The extra transistors enable the proposed testable circuit to operate like a pseudo static nMOS circuit while testing nMOS transistors in domino CMOS circuits. Therefore, the two=phase operation of a precharge phase and a evaluation phase is not needed to keep the domino CMOS circuit from malfunctionong due to circuit delays in the test mode, which reduces the testing time and the complexity of test generation. Most faults of th transistors in the proposed testable domino CMOS circuit can be detected by single test patterns. The use of single test patterns makes the testing of the proposed testable domino CMOS circuit free from path delays, timing skews, chage sharing and glitches. In the proposed design, the testing of the faults which, require test sequences also becomes free from test invalidation. The conventional automatic test pattern generators(ATPG) can be used for generating test patterns to detect faults in the circuits.