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MARGINAL MICROLEAKAGE OF SINGLE STEP ADHESIVES (단일 단계 접착제의 변연 미세누출)

  • Cho, Young-Gon;Jeong, Jin-Ho;Ki, Young-Jae;Choi, Hee-Young;Jin, Cheul-Hee;Yoo, Sang-Hoon;Kim, Jong-Uk;Park, Byung-Cheul
    • Restorative Dentistry and Endodontics
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    • v.29 no.2
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    • pp.162-169
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    • 2004
  • This study evaluated the marginal microleakage of five single step adhesives. Class V cavity preparations with occlusal margins in enamel and gingival margins in dentin were prepared on both buccal and lingual surfaces of extracted human molar teeth. Prepared teeth were randomly divided into five groups and restored using one of the single step adhesives and composite resins:Prompt L-Pop/Filtek Z-250 (Group 1), AQ Bond/Metafil CX (Group 2), One-Up Bond F/Palfique Toughwell (Group 3). Futurabond/Admira (Group 4), Xeno III/Spectrum TPH (Group 5). The restored teeth were thermocycled. Microleakage was assessed by dye penetration using 2% methylene blue dye solution. The teeth were bisected buccolingually and evaluated for microleakage under steromicroscope. The data were statistically analysed by Kruskal-Wallis test and Mann-Whitney tests. The results of this study were as follows:1. Microleakage of enamel margins in group 3 was statistically higher than that in groups 1, 2, 4, 5 (p < 0.05). 2. Microleakage of dentin margins in group 1 was statistically higher than that in groups 2, 5, and that in group 3 was statistically higher than that in groups 2, 4, 5 (p < 0.05). 3. Dentin marginal microleakage was higher than enamel marginal microleakage in all experimental groups. In conclusion, Prompt L-Pop showed the least leakage at enamel margin, and AQ Bond showed at dentin margin in this study. Marginal miroleakage in dentin was higher than that in enamel.

Reliability of Stereotactic Coordinates of 1.5-Tesla and 3-Tesla MRI in Radiosurgery and Functional Neurosurgery

  • Kim, Hae Yu;Lee, Sun-Il;Jin, Seong Jin;Jin, Sung-Chul;Kim, Jung Soo;Jeon, Kyoung Dong
    • Journal of Korean Neurosurgical Society
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    • v.55 no.3
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    • pp.136-141
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    • 2014
  • Objective : The aims of this study are to identify interpersonal differences in defining coordinates and to figure out the degree of distortion of the MRI and compare the accuracy between CT, 1.5-tesla (T) and 3.0T MRI. Methods : We compared coordinates in the CT images defined by 2 neurosurgeons. We also calculated the errors of 1.5T MRI and those of 3.0T. We compared the errors of the 1.5T with those of the 3.0T. In addition, we compared the errors in each sequence and in each axis. Results : The mean difference in the CT images between the two neurosurgeons was $0.48{\pm}0.22mm$. The mean errors of the 1.5T were $1.55{\pm}0.48mm$ (T1), $0.75{\pm}0.38$ (T2), and $1.07{\pm}0.57$ (FLAIR) and those of the 3.0T were $2.35{\pm}0.53$ (T1), $2.18{\pm}0.76$ (T2), and $2.16{\pm}0.77$ (FLAIR). The smallest mean errors out of all the axes were in the x axis : 0.28-0.34 (1.5T) and 0.31-0.52 (3.0T). The smallest errors out of all the MRI sequences were in the T2 : 0.29-0.58 (1.5T) and 0.31-1.85 (3.0T). Conclusion : There was no interpersonal difference in running the Gamma $Plan^{(R)}$ to define coordinates. The errors of the 3.0T were greater than those of the 1.5T, and these errors were not of an acceptable level. The x coordinate error was the smallest and the z coordinate error was the greatest regardless of the MRI sequence. The T2 sequence was the most accurate sequence.

Locating QTLs controlling overwintering seedling rate in perennial glutinous rice 89-1 (Oryza sativa L.)

  • Deng, Xiaoshu;Gan, Lu;Liu, Yan;Luo, Ancai;Jin, Liang;Chen, Jiao;Tang, Ruyu;Lei, Lixia;Tang, Jianghong;Zhang, Jiani;Zhao, Zhengwu
    • Genes and Genomics
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    • v.40 no.12
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    • pp.1351-1361
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    • 2018
  • A new cold tolerant germplasm resource named glutinous rice 89-1 (Gr89-1, Oryza sativa L.) can overwinter using axillary buds, with these buds being ratooned the following year. The overwintering seedling rate (OSR) is an important factor for evaluating cold tolerance. Many quantitative trait loci (QTLs) controlling cold tolerance at different growth stages in rice have been identified, with some of these QTLs being successfully cloned. However, no QTLs conferring to the OSR trait have been located in the perennial O. sativa L. To identify QTLs associated with OSR and to evaluate cold tolerance. 286 $F_{12}$ recombinant inbred lines (RILs) derived from a cross between the cold tolerant variety Gr89-1 and cold sensitive variety Shuhui527 (SH527) were used. A total of 198 polymorphic simple sequence repeat (SSR) markers that were distributed uniformly on 12 chromosomes were used to construct the linkage map. The gene ontology (GO) annotation of the major QTL was performed through the rice genome annotation project system. Three main-effect QTLs (qOSR2, qOSR3, and qOSR8) were detected and mapped on chromosomes 2, 3, and 8, respectively. These QTLs were located in the interval of RM14208 (35,160,202 base pairs (bp))-RM208 (35,520,147 bp), RM218 (8,375,236 bp)-RM232 (9,755,778 bp), and RM5891 (24,626,930 bp)-RM23608 (25,355,519 bp), and explained 19.6%, 9.3%, and 11.8% of the phenotypic variations, respectively. The qOSR2 QTL displayed the largest effect, with a logarithm of odds score (LOD) of 5.5. A total of 47 candidate genes on the qOSR2 locus were associated with 219 GO terms. Among these candidate genes, 11 were related to cell membrane, 7 were associated with cold stress, and 3 were involved in response to stress and biotic stimulus. OsPIP1;3 was the only one candidate gene related to stress, biotic stimulus, cold stress, and encoding a cell membrane protein. After QTL mapping, a total of three main-effect QTLs-qOSR2, qOSR3, and qOSR8-were detected on chromosomes 2, 3, and 8, respectively. Among these, qOSR2 explained the highest phenotypic variance. All the QTLs elite traits come from the cold resistance parent Gr89-1. OsPIP1;3 might be a candidate gene of qOSR2.

Varietal Differences in Agronomic Characters under Different Altitudinal Locations with Equal Latitude in Paddy Rice (동일위도상 표고의 차이에 따른 수도품종의 수량형질변이)

  • Park, S.Z.;Lee, E.W.;Lee, B.W.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.164-172
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    • 1983
  • The present study was conducted to investigate the variations in morphological traits, yield and yield-related components of rice cultivars exposed to different weather conditions by growing at the locations with different altitude but with equal latitude. Three Japonica varieties (Daegoldo, Jinheung, Shin #2) and three Tongil type semi-dwarf varieties(Josaengtongil, Suweon 287, wx 817-65-2-3) were seeded at Suweon on April 21 and transplanted at Suweon ($127^{\circ}$ N37 20', altitude 37m) and Jechun ($128.2^{\circ}$, N37 10', altitude 280-300m) on June 1 with the spacing of 30 $\times$ 15 em. The morphological traits, yield and yield-related components were measured. Culm length, paincle length and 1000-grain weight showed a little increase in Japonica varieties in Jechun as compared to those in Suweon and vice versa in semi-dwarf varieties, showing no significant differences. Number of panicles, per hill and number of spikelets per panicle among yield-related components showed remarkable increase in all varieties in Jechun as compared to those in 'Suweon but such inter-locational differences differed with varieties within varietal group and between varietal groups. On the contrary, grain fertility was higher in Suweon than that in Jechun and in Japonica varieties than that in semi-dwarf varieites , showing greater inter-locational difference in semi-dwarf varieties. Among Japonica varieties Jinheung and Shin #2 showed remarkable increase in yield in Jechun as compared to that in Suweon but in semidwarf varieties it was just the opposite. The importance of each of the yield-related component contributing to yield showed similar tendency 'regardless of locations in Japonica varieties. However, it was evidently different between locations in semidwarf varieties, where number of spikelets per panicle showed greatest direct effect on yield in Suweon but in Jechun so did grain fertility followed by the number of spikelets per panicle.

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Physiological Profile of Growing Rats: Effects of Cage Type and Cage Density

  • Yildiz, A.;Hayirli, A.;Okumus, Z.;Kaynar, O.;Kisa, F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.2
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    • pp.263-272
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    • 2007
  • This experiment was conducted to examine the effects of cage type (CT) and cage density (CD) on physiological variables in growing rats. Sprague Dawley rats (n=108) weighing an average of 46 g were housed in metallic cage with woodchip bedding (MCWB), metallic cage with wire mesh (MCWM), and plastic shoebox with woodchip bedding (PCWB) separately by sex at normal ($160-cm^2/rat$, ND) and high ($80-cm^2/rat$, HD) CD from 3 to 10 wks of age. All cages were in dimension of $24{\times}40{\times}20$ cm ($W{\times}D{\times}H$). At the end of the experiment, blood samples were collected and 6 rats from each cage were sacrificed. No death was observed among rats at ND, whereas mortality rate at HD was 22.3% for males and 13.9% for females. Heart weight was affected by CT. Doubling CD caused 23, 11.8, 17.9, 8.6, 6.9, and 16.4% decreases in BW and weights of heart, liver, kidney, testis, and ovary, respectively. Except for adrenal gland, other organs for males were heavier than for females. Liver weight of males and females responded differently to CT and CD. Comparing with females, males had 7.3 and 5.2% heavier and 9.9% lighter liver weights in MCWB, MCWM, and PCWB, respectively. As CD doubled, liver weight for males and females decreased by 22.4 and 13.1%, respectively. Mean adrenal gland weight increased by 8.4% and decreased by 9.7% for males and females, respectively, with doubling CD. CT affected glucose, TG, Ca, and ALP levels. However, CD did not alter blood chemistry. Rats housed in metallic cages had greater neutrophil count and neutrophil:lymphocyte ratio than rats housed in plastic cages. Doubling CD caused a 24.2% increase in lymphocyte count. There were CT by CD, CT by sex, and CD by sex interaction effects on lymphocyte count. Doubling CD caused 0.1% decrease and 49.8 and 26.7% increases in lymphocyte count for rats housed in MCWB, MCWM, and PCWB, respectively. Comparing with females, lymphocyte count for males housed in MCWB, MCWM, and PCWB had 8.9 and 12.9% greater and 30.3% less lymphocyte counts, respectively. Lymphocyte count decreased by 4.12% for males, whereas it increased by 61.0% for females as CD doubled. Doubling CD resulted in 2.5 and 2.3% increases in erythrocyte count and hematocrit value. These data suggest that animals perform better in metallic cages than in plastic cages and that cage density had pronounceable effects on physiological parameters in a cage type and sex dependent-manner.

Flora and Vegetation of Resources Plants in the Mt. Hansuk(Kangwon-do) (한석산의 자원식물상과 식생)

  • 백원기
    • Korean Journal of Plant Resources
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    • v.9 no.2
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    • pp.121-142
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    • 1996
  • This study was to establish the floristic composition and vegetation of resources vascular plants of Mt. Hansuk(1,119.1m; $128^{\circ}15';31'E,\;38^{\circ}02'55'N$). This work was conducted during the period of 1995. Resources vascular plants were composed of 85 families, 229 genera, 370 species, 72 varieties and 10 formae, totaling 452 taxa. Among the investigated resources plants, 15 taxa(2.6%) were Korean endemic species. The forest were comparatively well conserved by Pteridophyta calculation(Pte-Q), 1.34 Useful resources plants were edible source(43.8%), pasture source(38.1%), medicinal source(33.2%), ornamental source(11.9%) and timber source(4.9%) respectively. The forest vegetion was classified into 1 order, 1 alliances and 2 communities; Rhododendro - Quercetalia mongolicae Kim 1990 Lindero - Quercion mongolicae Kim 1990 A. Lespedeza maximowiczii - Quercus mongolica Community B. Carex humilis v. nana - Pinus densiflora Community

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Effect of dietary energy levels and phase feeding by protein levels on growth performance, blood profiles and carcass characteristics in growing-finishing pigs

  • Hong, J.S.;Lee, G.I.;Jin, X.H.;Kim, Y.Y.
    • Journal of Animal Science and Technology
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    • v.58 no.10
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    • pp.37.1-37.10
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    • 2016
  • Background: Providing of insufficient nutrients limits the potential growth of pig, while feeding of excessive nutrients increases the economic loss and causes environment pollution. For these reasons, phase feeding had been introduced in swine farm for improving animal production. This experiment was conducted to evaluate the effects of dietary energy levels and phase feeding by protein levels on growth performance, blood profiles and carcass characteristics in growing-finishing pigs. Methods: A total of 128 growing pigs ([Yorkshire ${\times}$ Landrace] ${\times}$ Duroc), averaging $26.62{\pm}3.07kg$ body weight, were assigned in a $2{\times}4$ factorial arrangement with 4 pigs per pen. The first factor was two dietary energy level (3,265 kcal of ME/kg or 3,365 kcal of ME/kg), and the second factor was four different levels of dietary protein by phase feeding (1growing(G)-2finishing(F) phases, 2G-2F phases, 2G-3F phases and 2G-3F phases with low CP requirement). Results: In feeding trial, there was no significant difference in growth performance. The BUN concentration was decreased as dietary protein level decreased in 6 week and blood creatinine was increased in 13 week when pigs were fed diets with different dietary energy level. The digestibility of crude fat was improved as dietary energy levels increased and excretion of urinary nitrogen was reduced when low protein diet was provided. Chemical compositions of longissimus muscle were not affected by dietary treatments. In backfat thickness ($P_2$) at 13 week, pigs fed high energy diet had thicker backfat thickness (P = 0.06) and pigs fed low protein diet showed the trend of backfat thinness reduction (P = 0.09). In addition, water holding capacity was decreased (P = 0.01) and cooking loss was increased (P = 0.07) as dietary protein level reduced. When pigs were fed high energy diet with low subdivision of phase feeding, days to 120 kg market weight was reached earlier compared to other treatments. Conclusion: Feeding the low energy diet and subdivision of growing-finishing phase by dietary protein levels had no significant effect on growth performance and carcass characteristics. Also, phase feeding with low energy and low protein diet had no negative effects on growth performance, carcass characteristics but economical profits was improved.

Stock Assessment and Management of Turban shell, Turbo (Batillus) cornutus Lightfoot, 1786 in Jeju Coastal waters, Korea (제주도산 소라 Turbo (Batillus) cornutus Lightfoot, 1786의 자원평가 및 관리방안 연구)

  • Kwon, Dae-Hyeon;Chang, Dae-Soo;Lee, Seung-Jong;Koo, Jun-Ho;Kim, Byung-Yeob
    • The Korean Journal of Malacology
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    • v.26 no.4
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    • pp.291-296
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    • 2010
  • Samples of Turbo (Batillus) cornutus Lightfoot, 1786 in Jeju Island were collected from September 2009 to May 2010. Population ecological parameters and stock assessment of the turban shell were determined, based on the length and age composition data from 2000 to 2009 and ecological parameters. Instantaneous coefficient of total mortality (Z) of turban shell was estimated to be 2.2062/year. The estimated instantaneous coefficient of natural mortality (M) was 0.874/year. The age of turban shell at its first capture ($t_c$) was 2.636 year. Yield-per-recruit were estimated under harvest strategies that based on $F_{max}$, $F_{0.1}$, $F_{35%}$, and $F_{40%}$ was 10.44 g, 1.87 g, 6.53 g and 7.46 g.

Analysis of Nutritional Compositions of the 7 kinds of Edible Wild Grasses (7종 식용야생초의 영양성분 분석)

  • Lee, Hei-Jung;Lee, Kyung-Hee;Ku, Sung-Ja
    • Korean journal of food and cookery science
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    • v.10 no.4
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    • pp.363-368
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    • 1994
  • The study was carried out to investigate the proximate composition, amino acids, dietary fiber, ascorbic acid and minerals contents of seven edible wild vegetables; an Evening Primerose($S_1$), a Spiderwort($S_2$), the Flower of a Convolvulus($S_3$), So Ru Jaeng Yi($S_4$), Shoe Bi Rum($S_5$), O Yi Pul($S_6$), Jip Sean Na Mul($S_7$). Results as summarized as follows: 1. The moisture contents of samples were determined as 64.43~88.30% The largest amount of them was contained in a Spiderwort($S_2$) and the smallest was in a Jip Sean Na Mul($S_7$), 2. The crude protein contents of samples were determined as 2.71~8.10%. The largest amount of them was contained in a O Yi Pul($S_8$), the smallest was in the Spiderwort($S_z$). 3. A relatively large amount of total amino acids were determined in samples. The abundant amino acids existed in the samples were Glutamic acid(10%), Lysine(8%), Leucine(8%) and the content of Methionine was very low. The highest level of them was found in the Convolvulus($S_3$), 4,419.6 mg% and the lowest was Shoe Bi Rum($S_5$), 2,393.1 mg% 4. The Ascorbic acid contents of samples was determined as 55.99~31.8O mg. The highest level of them was found in the Jip Sean Na Mul($S_7$) and the smallest was in the Primerose($S_1$) 5. The mineral contents of samples were determined. A relatively large amount of K, Ca and Mg were found in the samples. Especially the level of Iron was higher than that of any other vegetables. 6. The contents of dietary fiber were determined as 31.8~79.9%. The highest level of them was found in the Convolvulus($S_3$) and the lowest was in the Spiderwort($S_2$). The highest level of them was found in the Convolvulus($S_3$) and the lowest was in the Spiderwort($S_2$). From the above mention, we can say that the contents of protein, fat, fiber, total amino acids and iron were abundant in wild grasses than that of any other wild and cultured vegetables. The contents of ascorbic acid, potassium, calcium and magnesium were similar and the moisture is significantly low compared with any other vegetables.

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Nutritional Evaluation of Bamboo Shoot Shell and Its Effect as Supplementary Feed on Performance of Heifers Offered Ammoniated Rice Straw Diets

  • Liu, J.X.;Wang, X.Q.;Shi, Z.Q.;Ye, H.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.10
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    • pp.1388-1393
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    • 2000
  • The present study was conducted to examine the feasibility of utilising bamboo (Bambusa arundinacea) shoot shell (BSS) in ruminants. Chemical composition, rumen degradability and some antinutritional compounds were determined for fresh and boiled BSSs to evaluate its feed value and safety. Thirty-two Holstein heifers were allocated to four groups and used to investigate the response in growth rate to supplementing ammoniated rice straw with fresh shell (phase 1) or silage of boiled BSS (phase 2). All animals were offered ammoniated straw ad libitum with 1kg of cotton seed meal (phase 1) or 0.5 kg of cotton seed meal and 0.5 kg of concentrate mixture (phase 2) per head per day. The BSS was supplemented at levels of 0, 3, 6 or 9 kg/d (phase 1) and 0, 5, 10 or 15 kg/d (phase 2) (as fed basis). The BSS was very high in moisture content, and its contents of crude protein and neutral detergent fiber were 13~16% DM and 65~76% DM, respectively; boiling resulting in higher moisture and protein. No hydrocyanic acid was detected in both BSSs and content of tannins was negligible. Rumen degradability of BSS was reasonably high, and with boiling the rapidly degradable fraction decreased, and potentially degradable component increased. Silage of the boiled BSS was slightly lower in both rapidly and slowly degraded fractions than the fresh BSS. Animals consumed all supplemented BSSs without any adverse health problems. Intake of ammoniated straw decreased with the increasing levels of BSS, but total intake was higher in almost all supplementary groups than in the non-BSS. Heifers had a higher growth rate in phase 1 with fresh BSS than in phase 2 with ensiled shell, and daily weight gains were 622, 629, 744 or 690 g in phase 1, and 578, 575, 677 or 635 g in phase 2 at four BSS levels, respectively. For both phases growth rate was significantly higher for the animals in groups 3 and 4 than those in groups 1 and 2 (p<0.01), with little difference between groups 1 and 2 (p>0.05) but significant difference between groups 3 and 4 (p<0.05). Supplementation with BSS also resulted in an improved feed conversion rate, with the least concentrate consumption in group 3 for both phases. It is concluded that the BSS has a high potential nutritional value as indicated by its medium protein content, reasonably high rumen degradability, and that inclusion of BSS in ammoniated rice straw diet is not only safe to animals, but also may improve growth rate of ruminants and feed conversion rate. It may be disadvantageous to use high amounts of BSS in ammoniated straw diets.