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Specific Primer for Detection of Jujube Witches' Broom Phytoplasma Group (16SrV) in Korea

  • Han, Sang-Sub
    • The Plant Pathology Journal
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    • v.21 no.1
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    • pp.55-58
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    • 2005
  • In order to diagnose and differentiate jujube witches' broom (JWB) phytoplasma rapidly, oligonucleotide primer pair, 16Sr(V) F/R, for polymerase chain reactions (PCRs) was designed on the basis of 16S rRNA sequences of JWB phytoplasma. The PCR employing phytoplasma universal primer pair P1/P7 consistently amplified DNA in all tested phytoplasma isolates. But no phytoplasma DNA was detected from healthy jujube seedlings. The nested PCR, the primer pair 16S(V) F/R, about 460 bp fragment, amplified DNA in all tested JWB and related phytoplasmas including ligustrum witches' broom phytoplasma of the 16S rRNA group V, but no DNA amplification was detected from other phytoplasma strains such as groups 16SrI (Aster yellows) and 16SrXII (Stolbur group) in which mulberry dwarf phytoplasma and chrysanthemum witches' broom phytoplasma belong to, respectively. The same results were obtained from both Korean and Chinese isolates of JWB phytoplasma. Nested-PCR using phytoplasma universal primer pair P1/P7 and 16SrV group-specific primer pair 16S(V) F/R could detect group V phytoplasmas rapidly and easily, in particular JWB phytoplasma.

Effect of Ginseng Saponin on Hypothalamus-Pituitary- Adrenal Axis under Stress in Mice

  • Do Hoon Kim;Jun
    • Proceedings of the Ginseng society Conference
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    • 1998.06a
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    • pp.83-89
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    • 1998
  • Ginseng total saponins (GTS) injected intracerebroventricularly (i.c.v.) at doses from 0.1-1 vs inhibited the i.c.v. injection stress-induced plasma corticosterone levels in mice. The inhibitory action of GTS was blocked by co-administered NG-nitro-L-arginine methyl ester (L-NAME; 1.5 us, i.c.v.), an. inhibitor of nitric oxide synthase (NOS). Of the ginsenosides Rbl, Rba, Rc, Rd, Re, Rf, Rgl,20(S)-Rg3, and 20(R)-Rg3 injected i.c.v. at doses from 0.01 to 0.3ug(or 1 uE),20(5)-Rg3 and Rc significantly inhibited the o.c.v. injection stress-induced Plasma corticosterone levels. The inhibitory actions of 20(S)-Rg3 and Rc were blocked by co-administered L-NAME (1.5 n, i.c.v.). These results suggest that G75, 20(S)-Rg3 and Rc may inhibit the i.c.v. injection stress-induced hypothalamo-pituitary-adrenal response by inducing NO production in the brain.

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Application of 50-kV Inverter System for Testing a Characteristics of home-made 80-MW Pulsed Klystron (80 MW급 클라이스트론 성능시험을 위한 50 kV 인버터 시스템의 적용)

  • Jang S. D.;Son Y. G.;Oh J. S.
    • Proceedings of the KIEE Conference
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    • summer
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    • pp.1172-1175
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    • 2004
  • 포항 방사광 가속기의 선형가속기는 2.5GeV 전자빔용 마이크로웨이브의 발생을 위하여 80 MW급 클라이스트론(klystron) 11대와 입사부용 65 MW 급 클라이스트론 1대를 사용한다. 80 MW 급 클라이스트론 부하를 구동하기 위하여 최대 펄스 정격출력 200MW(400kV, 500A, 평탄도 $4.4\;{\mu}s$)인 대출력 펄스 전원공급 장치(modulator)가 요구된다. 모듈레이터 시스템 용 PFN(pulse forming network) 커패시터의 충전용 입력전원으로써 최대 출력전압 50 kV, ${\pm}\;0.5\%$ 이내의 전압제어가 가능한 고전압 인버터 전원장치를 적용하여 PLS 선형가속기용 국산화 개발 클라이스트론 부하의 성능시험을 수행하였다. 국산화 개발된 S-band 펄스 클라이스트론은 고전압 길들이기 과정을 거쳐 최대 정격 출력 빔 전압 400 kV 이상까지 시험 완료하였다. 클라이스트론의 정확한 RF 출력성능의 측정을 위하여 방향성 결합기와 검파기를 설치하여 측정시스템의 성능을 개선하였다. 본 논문에서는 포항 방사광 가속기의 국산화 개발 1호 클라이스트론 부하의 성능시험을 위한 50 kV 급 인버터시스템 적용과정에서 수행하였던 시험장치 개선과 PFN의 충전 특성을 분석하였다. 또한, 클라이스트론의 고전압 및 RF 길들이기 시험 결과에 관하여 고찰하고자 한다.

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Superovulation in Korea Cattle with a Single Subcutaneous Injection of Folltropin-V Dissolved in Polyethyleneglycol (한우에 있어서 PEG에 용해시킨 Folltropin-V의 1회 피하주사에 의한 다배란 유기)

  • 임석기;우제석;전기준;장선식;강수원;윤상기;손동수
    • Journal of Embryo Transfer
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    • v.13 no.3
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    • pp.207-212
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    • 1998
  • This study was conducted to determined if single subcutaneous (s.c.) injection of Folltropin-V dissolved in polyethyleneglycol (PEG) can replace as the standard multiple intramuscular (i.m.) injection. The results suggest that the s.c. treatment produced more corpora lutea, embryos recovered and transferable embryos as compared to the i.m. treatment (p<0.05). This study indicates that a single s.c. injection of Folltropin-V dissolved in PEG was effective for superovulatory response and embryo yield in Korean cattle.

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The Pressure Effect on the Ionic Association of the 3,5,N-trimethyl Pyridinium Iodide in Ethanol-Water Mixture

  • Jong-Gi Jee;Young Hwa Lee;Kyung-Hee Lee;Oh Cheun Kwun
    • Bulletin of the Korean Chemical Society
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    • v.5 no.3
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    • pp.112-117
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    • 1984
  • The association constants (K) of 3,5,N-trimethyl pyridinium iodide in 95 volume percent ethanol-water mixed solvent were determined by a modified UV and conductance method at $25^{\circ},\;30{\circ},\;40{\circ}\;and\;50{\circ}C$ over the pressure range 1 to 2000 bars. The association process is enhanced with increasing pressure and decreasing temperature. From K values, we obtained the total partial molar volume change (${\Delta}V$) and some thermodynamic parameters. The electrostriction volume (${\Delta}V_{el}$) and intrinsic volume (${\Delta}V_{in}$) were also evaluated. The values of ${\Delta}V,\;{\Delta}V_{el},\;{\Delta}V_{in}$ are negative, negative and positive, respectively, and the absolute values of all these three decrease with increasing pressure and temperature. The ion-pair size (a) were varied 3 to 6 ${\AA}$, with pressure and temperature. The solvation number (n) decreased from 2 to 0.5 with increasing temperature.

BVRI Standardization of the CCD Photometric System of Sobaeksan Optical Astronomy Observatory (소백산 천문대 CCD 측광계의 BVRI 표준화)

  • Jeong, Jang-Hae;Kim, Chun-Hwey;Lee, Yong-Sam
    • Journal of Astronomy and Space Sciences
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    • v.25 no.2
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    • pp.87-100
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    • 2008
  • A total of 792 CCD images of V523 Cas were obtained on four nights of Jan. 2003 with the bvri CCD photometric system attached to a 61cm reflector of Sobaeksan Optical Astronomy Observatory (SOAO). The 17 standard stars in the images were used to establish transformation relations between our bvri system and the standard Johnson-Cousins BVRI system. We derived the tentative equations of transformation between two photometric systems as follows; V=v-0.0689(B-V)+0.0063, B-V=1.3197(b-v)-0.1733, V-R=0.9210(v-r)-0.1309, R-I=0.8892(r-i)-0.1055. Using these equations standard V magnitudes and their color indexes (B-V, V-R, R-I) for 57 stars in the field of the image were determined.

Effects of V and C additions on the Thermal Expansion and Tensile Properties of a High Strength Invar Base Alloy (고강도 인바계 합금의 열팽창 및 인장 특성에 미치는 바나듐과 탄소 원소 첨가 영향)

  • Yun, A.C.;Yun, S.C.;Ha, T.K.;Song, J.H.;Lee, K.A.
    • Transactions of Materials Processing
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    • v.24 no.1
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    • pp.44-51
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    • 2015
  • The current study seeks to examine the effects of V and C additions on the mechanical and low thermal expansion properties of a high strength invar base alloy. The base alloy (Fe-36%Ni-0.9%Co-2.75%Mo-0.7Cr-0.23Mn-0.17Si-0.3%C, wt.%) contains $Mo_2C$ carbides, which form as the main precipitate. In contrast, alloys with additions of 0.4%V+0.3%C (alloy A) or 0.4%V+0.45%C (alloy B) contain $Mo_2C$+[V, Mo]C carbides. The average thermal expansion coefficients of these high strength invar based alloys were measured in the range of $5.16{\sim}5.43{\mu}m/m{\cdot}^{\circ}C$ for temperatures of $15{\sim}230^{\circ}C$. Moreover, alloy B showed lower thermal expansion coefficient than the other alloys in this temperature range. For the mechanical properties, the [V, Mo]C improved hardness and strengths(Y.S. and T.S.) of the high strength invar base alloy. T.S.(tensile strength) and Y.S.(yield strength) of hot forged alloy B specimen were measured at 844.6MPa and 518.0MPa, respectively. The tensile fractography of alloy B exhibited a ductile transgranular fracture mode and voids were initiated between the [V, Mo]C particles and the matrix. Superior properties of high strength and low thermal expansion coefficient can be obtained by [V, Mo]C precipitation in alloy B with the addition of 0.4%V and 0.45%C.

Antiviral Treatment Reveals a Cooperative Pathogenicity of Baculovirus and Iflavirus in Spodoptera exigua, a Lepidopteran Insect

  • Roy, Miltan Chandra;Ahmed, Shabbir;Mollah, Md. Mahi Imam;Kim, Yonggyun
    • Journal of Microbiology and Biotechnology
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    • v.31 no.4
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    • pp.529-539
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    • 2021
  • The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various vegetable crops. Two infectious insect viruses, baculovirus and iflavirus, are known to induce epizootics in S. exigua populations. Indeed, some laboratory colonies have appeared to be covertly infected by these viruses. Diagnostic PCR tests detected two different viruses: Spodoptera exigua multiple nucleopolyhedrosis virus (SeMNPV) and iflaviruses (SeIfV1 and SeIfV2). Viral extract from dead larvae of S. exigua could infect Sf9 cells and produce occlusion bodies (OBs). Feeding OBs to asymptomatic larvae of S. exigua caused significant viral disease. Interestingly, both SeIfV1 and SeIfV2 increased their titers at late larval stages. Sterilization of laid eggs with 1% sodium hypochloride significantly reduced SeMNPV titers and increased larval survival rate. Double-stranded RNA (dsRNA) specific to SeIfV1 or SeIfV2 significantly reduced viral titers and increased larval survival rate. To continuously feed dsRNA, a recombinant Escherichia coli HT115 expressing SeIfV1-dsRNA was constructed with an L4440 expression vector. Adding this recombinant E. coli to the artificial diet significantly reduced the SeIfV1 titer and increased larval survival. These results indicate that laboratory colony collapse of S. exigua is induced by multiple viral infections. In addition, either suppression of SeMNPV or SeIfV infection significantly increased larval survival, suggesting a cooperative pathogenicity between baculovirus and iflavirus against S. exigua.

Dust Collection Characteristics of Multi-layer Multi-stage Porous Plate System with Polarization Charge to Impaction Effect (임팩션 효과에 편극전하 방식을 부가한 다층 다단 다공성 플레이트 시스템의 집진특성)

  • Kim, Bo-Bae;Kim, Il-Kyu;Yoa, Seok-Jun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.8
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    • pp.598-605
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    • 2011
  • The main object of this study is to investigate the Dust Collection Characteristics of multi-layer multi-stage porous plate system with polarization charging mechanism, experimentally. The experiment is carried to analyze the characteristics of pressure drop and collection efficiency for the present system with the experimental parameters such as applied voltage, inlet velocity, inlet concentration and stage number, etc. In results, the pressure drop becomes 18 to $134mmH_2O$, with increment of stage number (1 to 5) at inlet velocity $v_{in}$ = 3.11 m/s ($v_t$ = 18 m/s) and inlet concentration 3 g/m3 for inflow current. In case of both applied voltage 0 kV and non-inflow current, the collection efficiency of 5 stage is 92.5% at $v_{in}$ = 2.58 m/s ($v_t$ = 15 m/s), while it is estimated that the collection efficiency becomes about 6% higher than that of 0 kV and non-inflow as 98.5% at $v_{in}$ = 2.58 m/s for both alternating polarization charge (applied voltage 2.5 kV) and inflow current system.