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http://dx.doi.org/10.5423/PPJ.2005.21.1.055

Specific Primer for Detection of Jujube Witches' Broom Phytoplasma Group (16SrV) in Korea  

Han, Sang-Sub (Division of Forest Science, Chonbuk National University)
Publication Information
The Plant Pathology Journal / v.21, no.1, 2005 , pp. 55-58 More about this Journal
Abstract
In order to diagnose and differentiate jujube witches' broom (JWB) phytoplasma rapidly, oligonucleotide primer pair, 16Sr(V) F/R, for polymerase chain reactions (PCRs) was designed on the basis of 16S rRNA sequences of JWB phytoplasma. The PCR employing phytoplasma universal primer pair P1/P7 consistently amplified DNA in all tested phytoplasma isolates. But no phytoplasma DNA was detected from healthy jujube seedlings. The nested PCR, the primer pair 16S(V) F/R, about 460 bp fragment, amplified DNA in all tested JWB and related phytoplasmas including ligustrum witches' broom phytoplasma of the 16S rRNA group V, but no DNA amplification was detected from other phytoplasma strains such as groups 16SrI (Aster yellows) and 16SrXII (Stolbur group) in which mulberry dwarf phytoplasma and chrysanthemum witches' broom phytoplasma belong to, respectively. The same results were obtained from both Korean and Chinese isolates of JWB phytoplasma. Nested-PCR using phytoplasma universal primer pair P1/P7 and 16SrV group-specific primer pair 16S(V) F/R could detect group V phytoplasmas rapidly and easily, in particular JWB phytoplasma.
Keywords
jujube witches' broom; oligonucleotide primer; PCR; phytoplasma;
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1 Ahrens, U. and Seemuller, E. 1992. Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82:828-832   DOI
2 Schneider, B., Seemuller, E., Smart, C. D. and Kirkpatrick, B. C. 1995. Phylogenetic classification of plant pathogenic mycoplasma like organism or phytoplasmas. In: Molecular and Diagnostic procedures in Mycoplasmology, ed. by R. Razin and J. G. Tully, pp. 1:369-380. San Diego, CA: Academic Press
3 Deng, S. J. and Hiruki, C. 1991. Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. J. Microbiol. Methods 14:53-61   DOI   ScienceOn
4 Lee, I.-M. and Davis, R. E. 1988. Detection and investigation of genetic relatedness among aster yellows and other myco-plasma-like organisms using cloned DNA and RNA probes. Mol. Plant-Microbe Interact. 1:303-310   DOI
5 Montano, H. G., Davis, R. E., Dally, E. L., Hogenhout, S., Pimentel, J. P. and Brioso, P. S. T. 2001. 'Candidatus phytoplasma brasiliense' a new phytoplasma taxon associated hibiscus witches' broom disease. Int. J. Syst. Evol. Microbiol. 51:1109- 1118   DOI   ScienceOn
6 Gundersen, D. E., Lee, I. M., Rehner, S. A., Davis, R. E. and Kingsbury, D. T. 1994. Phylogeny of mycoplasma organisms (phytoplasmas): A base for their classification. J. Bacteriol. 176:5244-5254   DOI
7 Daniel, T. W., Linda, L. B., Paul, T. S. and Kerry, B. W. 1998. Phylogenetic position of phytoplasmas associated with dieback, yellow crinkle and mosaic diseases of papaya, and their proposed inclusion in 'Candidatus phytoplasma australiense' and a new taxon, 'phytoplasma australasia'. Int. J. Syst. Evol. Microbiol. 48:941-951   DOI
8 Hong, S. W. and Kim, C. J. 1960. Witches' broom of jujube tree (Zizyphus jujube Mill. var. inermis Rehd.). (I) On symptom and disease name. Kor. J. Botany 3:32-38
9 Davis, R. E. and Lee, I.-M. 1993. Cluster-specific polymerase chain reaction amplification of 16S rDNA sequences for detection and identification of mycoplasmalike organisms. Phytopathology 83:772-776   DOI   ScienceOn
10 Lee, I.-M., Davis, R. E. and Gundersen, D. E. 2000. Phytoplasma: Phytopathogenic mollicutes. Annu. Rev. Microbiol. 54:221- 255   DOI   ScienceOn
11 Han, S. S. and Cha, B. 2002. Genetic similarity between jujube witches' broom and mulberry dwarf phytoplasmas transmitted by Hishimonas sellatus Uhler. Kor. J. Plant Pathol. 18:98-101   DOI   ScienceOn
12 Kollar, A., Seemuller, E., Bonet, F., Saillaird, S. and Bove, J. M. 1990. Isolation of the DNA of various plant pathogenic mycoplasmalike organisms from infected plants. Phytopathology 80:3233-3237
13 Lee, I.-M., Gundersen, D. E., Hannond, R. W. and Davis, R. E. 1994. Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84:559-565   DOI   ScienceOn
14 Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E. and Bartoszyk, I. M. 1998. Revised classification scheme of phytoplasmas based on RFLP analysis of 16S rRNA and ribsomal protein gene sequences. Int. J. Syst. Bacteriol. 48:1153-1169   DOI
15 Deng, S. J. and C. Hiuki. 1990. The use of cloned DNA probes for diagnosis of noncultivable plant mollicutes. Proc. Japan Acad. 66B:58-61
16 Davis, R. E., Dally, E. L., Gundersen, D. E., Lee, I.-M. and Habili, N. 1997. 'Candidatus phytoplasma australiense' a new phytoplasma taxon associated with Australian grapevine yellows. Int. J. Syst. Evol. Microbiol. 47:262-269   DOI
17 Kim, C. J. 1965. Witches' broom of jujube tree (Zizyphus jujube Mill. var. inermis Rehd.). (IV) Transmission by grafting. Kor. J. Microbiol. 3:1-6
18 Kim, C. J. 1968. Witches' broom of jujube tree (Zizyphus jujube Mill. var. inermis Rehd.). Res. Bull. Choonchon Agric. College 2:47-53